To explore the effect of [-Ca^2＋]i and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus （MCMV） infected mice, newborn Balb/c mice were randomly divided into two groups： a vir...To explore the effect of [-Ca^2＋]i and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus （MCMV） infected mice, newborn Balb/c mice were randomly divided into two groups： a virus inoculated group and a control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [-Ca^2＋]i in hippocampus were measured by means of flow cytometry （FCM）. Compared with the control group, the mitochondria transmembrane potentials was decreased （P〈0. 01 ） and the intracellular free calcium level [-Ca^2＋]i was increased （P〈0. 01） in inoculated group. The dysfunction of [-Ca^2＋]i and mitochondria transmembrane potentials in hippocampus may play an important role in the functional disorders in CMV-infected CNS.展开更多
We previously reported that ONO-AE-248, a selective EP3 receptor agonist, has been shown to cause neutrophil death without the typical features of apoptosis and necrosis. However, the mechanism of the neutrophil death...We previously reported that ONO-AE-248, a selective EP3 receptor agonist, has been shown to cause neutrophil death without the typical features of apoptosis and necrosis. However, the mechanism of the neutrophil death is unclear. By using Western blotting, flow cytometry （FACS） and confocal laser scanning microscopy （CLSM）, we investigated the cellular signal transduction pathways of the neutrophil death. The research results showed that the neutrophil death induced by ONO-AE-248 did not show the morphologic changes of apoptosis and was not associated with the activity of caspase-3, caspase-8, and phosphorylation of p38-MAPK. However, impairment of mitochondria transmembrane potential has been found during the process of cell death. These findings suggested that ONO-AE-248 induced a non-apoptotic programmed cell death of neutrophils through partially mitochondria signaling transduction pathway. Cellular ＆ Molecular Immunology.展开更多
基金This project was supported by a grant from the NationalNatural Sciences Foundation of China (No .30271673)
文摘To explore the effect of [-Ca^2＋]i and neuronal mitochondria transmembrane potentials in hippocampus of murine cytomegalovirus （MCMV） infected mice, newborn Balb/c mice were randomly divided into two groups： a virus inoculated group and a control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [-Ca^2＋]i in hippocampus were measured by means of flow cytometry （FCM）. Compared with the control group, the mitochondria transmembrane potentials was decreased （P〈0. 01 ） and the intracellular free calcium level [-Ca^2＋]i was increased （P〈0. 01） in inoculated group. The dysfunction of [-Ca^2＋]i and mitochondria transmembrane potentials in hippocampus may play an important role in the functional disorders in CMV-infected CNS.
基金supported by grants from the National Natural Science Foundation of China(No.30371305).
文摘We previously reported that ONO-AE-248, a selective EP3 receptor agonist, has been shown to cause neutrophil death without the typical features of apoptosis and necrosis. However, the mechanism of the neutrophil death is unclear. By using Western blotting, flow cytometry （FACS） and confocal laser scanning microscopy （CLSM）, we investigated the cellular signal transduction pathways of the neutrophil death. The research results showed that the neutrophil death induced by ONO-AE-248 did not show the morphologic changes of apoptosis and was not associated with the activity of caspase-3, caspase-8, and phosphorylation of p38-MAPK. However, impairment of mitochondria transmembrane potential has been found during the process of cell death. These findings suggested that ONO-AE-248 induced a non-apoptotic programmed cell death of neutrophils through partially mitochondria signaling transduction pathway. Cellular ＆ Molecular Immunology.
