AIM: To determine whether normal genetically immunocompetent rodent hosts could be manipulated to accept human hepatocyte transplants with long term survival without immunosuppression. METHODS: Tolerance towards human...AIM: To determine whether normal genetically immunocompetent rodent hosts could be manipulated to accept human hepatocyte transplants with long term survival without immunosuppression. METHODS: Tolerance towards human hepatocytes was established by injection of primary human hepatocytes or Huh7 human hepatoma cells into the peritoneal cavities of fetal rats. Corresponding cells were subsequently transplanted into newborn rats via intrasplenic injection within 24h after birth. RESULTS: Mixed lymphocyte assays showed that spleen cells from non-tolerized rats were stimulated to proliferate when exposed to human hepatocytes, while cells from tolerized rats were not. Injections made between 15 d and 17 d of gestation produced optimal tolerization. Transplanted human hepatocytes in rat livers were visualized by immunohistochemical staining of human albumin. By dot blotting of genomic DNA in livers of tolerized rats 16 weeks after hepatocyte transplantation, it was found that approximately 2.5 X 10(5) human hepatocytes survived per rat liver. Human albumin mRNA was detected in rat livers by RT-PCR for 15 wk, and human albumin protein was also detectable in rat serum. CONCLUSION: Tolerization of an immuno-competent rat can permit transplantation, and survival of functional human hepatocytes.展开更多
The model for computing frictional coefficient between two teeth faces at the state of mixed elastohydrodynamic lubrication is established. And then more than 80 sets of numerical calculations and six sets of disc fat...The model for computing frictional coefficient between two teeth faces at the state of mixed elastohydrodynamic lubrication is established. And then more than 80 sets of numerical calculations and six sets of disc fatigue tests are completed. The results show that when the film thickness ratio λ 〈1.6, frictional coefficient μ is drastically decreased as λ. rises; Thereafter it decreases smoothly until λ=4.5. When λ〉4.5, however, it goes up again with λ, which indicates that the excessive film thickness ratio will deteriorate gearing contact fatigue strength. At the end, the formulae for determining the frictional coefficients are formed.展开更多
Rutting or permanent deformation is one of the major distress modes of hot mix asphalt in the field. Triaxial cycle compression testing （TCCT） is a standardized and scientifically accepted test method to address thi...Rutting or permanent deformation is one of the major distress modes of hot mix asphalt in the field. Triaxial cycle compression testing （TCCT） is a standardized and scientifically accepted test method to address this distress mode in the lab and to characterize the resistance to permanent deformation. In most labs and according to EN 12697-25, standard TGCTs are carried out with cyclic axial loading and a constant confining pressure. In road pavements on the other hand, dynamic traffic loading due to passing wheels leads to cyclic confining pressure. In order to bring the TCCT closer to reality, the radial reaction and its phase lag to axial loading in standard TCCTs are analyzed and an enhanced TCCT with cyclic confining pressure is introduced. The cyclic confining pressure takes into account the viscoelastic material response by the radial phase lag to axial phase loading. In a subsequent test program, TCCTs with different confining pressure amplitudes were carried out on two hot mix asphalts. Results from standard and enhanced TCCTs were analyzed, compared and discussed. It is shown that the resistance to permanent deformation in- creases significantly when the viscoelastic material response is taken into account in the TCCT by introducing cyclic confining pressure.展开更多
文摘AIM: To determine whether normal genetically immunocompetent rodent hosts could be manipulated to accept human hepatocyte transplants with long term survival without immunosuppression. METHODS: Tolerance towards human hepatocytes was established by injection of primary human hepatocytes or Huh7 human hepatoma cells into the peritoneal cavities of fetal rats. Corresponding cells were subsequently transplanted into newborn rats via intrasplenic injection within 24h after birth. RESULTS: Mixed lymphocyte assays showed that spleen cells from non-tolerized rats were stimulated to proliferate when exposed to human hepatocytes, while cells from tolerized rats were not. Injections made between 15 d and 17 d of gestation produced optimal tolerization. Transplanted human hepatocytes in rat livers were visualized by immunohistochemical staining of human albumin. By dot blotting of genomic DNA in livers of tolerized rats 16 weeks after hepatocyte transplantation, it was found that approximately 2.5 X 10(5) human hepatocytes survived per rat liver. Human albumin mRNA was detected in rat livers by RT-PCR for 15 wk, and human albumin protein was also detectable in rat serum. CONCLUSION: Tolerization of an immuno-competent rat can permit transplantation, and survival of functional human hepatocytes.
基金This project is supported by Provincial Natural Science Foundation of Shanxi, China (No. 20041057)Scholarship Council of Shanxi, China (No. 2005-22)
文摘The model for computing frictional coefficient between two teeth faces at the state of mixed elastohydrodynamic lubrication is established. And then more than 80 sets of numerical calculations and six sets of disc fatigue tests are completed. The results show that when the film thickness ratio λ 〈1.6, frictional coefficient μ is drastically decreased as λ. rises; Thereafter it decreases smoothly until λ=4.5. When λ〉4.5, however, it goes up again with λ, which indicates that the excessive film thickness ratio will deteriorate gearing contact fatigue strength. At the end, the formulae for determining the frictional coefficients are formed.
文摘Rutting or permanent deformation is one of the major distress modes of hot mix asphalt in the field. Triaxial cycle compression testing （TCCT） is a standardized and scientifically accepted test method to address this distress mode in the lab and to characterize the resistance to permanent deformation. In most labs and according to EN 12697-25, standard TGCTs are carried out with cyclic axial loading and a constant confining pressure. In road pavements on the other hand, dynamic traffic loading due to passing wheels leads to cyclic confining pressure. In order to bring the TCCT closer to reality, the radial reaction and its phase lag to axial loading in standard TCCTs are analyzed and an enhanced TCCT with cyclic confining pressure is introduced. The cyclic confining pressure takes into account the viscoelastic material response by the radial phase lag to axial phase loading. In a subsequent test program, TCCTs with different confining pressure amplitudes were carried out on two hot mix asphalts. Results from standard and enhanced TCCTs were analyzed, compared and discussed. It is shown that the resistance to permanent deformation in- creases significantly when the viscoelastic material response is taken into account in the TCCT by introducing cyclic confining pressure.
