解磷细菌PSB3的筛选及拮抗作用的研究

利用有机磷细菌液体培养基进行生物富集,无机磷细菌固体培养基通过平板稀释法进行分离筛选,建立了土壤解磷细菌的筛选体系。扩增菌株PSB3的16SrDNA序列,序列测定结果显示,该片段长度为1525bp,经Blastn搜索进行序列比对,该细菌为洋葱伯克霍尔德氏菌(Burkholderia cepacia)。对该菌株与供试的12个炭疽菌和镰刀菌菌株进行室内拮抗试验,结果显示,该菌株对Fusarium solani等6个菌株有不同程度的拮抗作用。

水杉赤枯病病原形态及分子鉴定

对上海、南京地区的水杉赤枯病(Metasequoia red blight)的发生情况进行了调查和分析,并采用形态学和分子生物学结合的方法对该病的病原菌进行了鉴定。结果表明:单行种植和单棵零星分布的水杉感病程度相对于已经郁闭成林的水杉要严重得多;用作行道树的水杉抗病能力较弱,发病情况相对于农田中的水杉较为严重;生长势弱的水杉植株容易发生赤枯病,这一现象在行道树中表现尤为明显。通过对水杉赤枯病病原菌分离培养、人工接种试验、分离菌形态学观察及分子生物学分子鉴定,水杉赤枯病的病原菌被鉴定为细极链格孢(Alternaria tenuissima)和异角状拟盘多毛孢(Pestalotiopsis heterocornis)。

小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定

本研究旨在建立小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱的分子生物学鉴定方法,并探讨它们的系统发生关系。在新疆、内蒙古从动物体表采集寄生蜱,形态学鉴定后,PCR扩增测序获得3种璃眼蜱的16SrRNA及线粒体色素氧化酶亚基Ⅰ基因(cytochrome oxidaseⅠ,COⅠ)序列后进行同源性分析。用Mega 5.0和Mrbayes 3.2软件分别构建系统进化树,亚洲璃眼蜱、小亚璃眼蜱样本16SrRNA序列与GenBank中已知相应蜱虫16SrRNA序列聚类,而残缘璃眼蜱COⅠ序列与GenBank中已知残缘璃眼蜱COⅠ序列聚类,与形态学鉴定结果一致。在传统形态学分类的基础上,结合分子生物学鉴定方法能简易、准确鉴定小亚璃眼蜱、亚洲璃眼蜱和残缘璃眼蜱。

Preparation of Composite Microporous Silica Membranes Using TEOS and 1,2-Bis(triethoxysilyl)ethane as Precursors for Gas Separation

This paper reports on a new microporous composite silica membrane prepared via acid-catalyzed polymeric route of sol-gel method with tetraethylorthosilicate(TEOS)and a bridged silsesquioxane[1,2-bis(triethoxysilyl)ethane, BTESE]as precursors.A stable nano-sized composite silica sol with a mean volume size of^5 nm was synthesized. A 150 nm-thick defect-free composite silica membrane was deposited on disk support consisting of macroporous α-Al2O3 and mesoporousγ-Al2O3 intermediate layer by using dip-coating approach,followed by calcination under pure nitrogen atmosphere.The composite silica membranes exhibit molecular sieve properties for small gases like H2,CO2,O2,N2,CH4 and SF6 with hydrogen permeances in the range of(1-4)×10 -7mol·m -2·s -1·Pa -1(measured at 200°C,3.0×105 Pa).With respect to the membrane calcined at 500°C,it is found that the permselectivities of H 2 (0.289 nm)with respect to N2(0.365 nm),CH4(0.384 nm)and SF6(0.55 nm)are 22.9,42 and>1000,respectively, which are all much higher than the corresponding Knudsen values(H2/N2=3.7,H2/CH4=2.8,and H2/SF6=8.5).

Preparation, Characterization, Biological Activity and 3D Molecular Modeling of Mn（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ）, Cu（Ⅱ）, Pd（Ⅱ） and Ru（Ⅲ） Complexes of Some Sulfadrug Schiff Bases

Mn（Ⅱ）, Co（Ⅱ）, Ni（Ⅱ）, Cu（Ⅱ）, Pd（Ⅱ） and Ru（Ⅲ） complexes of Schiff bases derived from the condensation of sulfaguanidine with 2,4-dihydroxy benzaldehyde （HL1）, 2-hydroxy-l-naphthaldehyde （HL2） and salicylaldehyde （HL3） have been synthesized. The structures of the prepared metal complexes were proposed based on elemental analysis, molar conductance, thermal analysis （TGA, DSC and DTG）, magnetic susceptibility measurements and spectroscopic techniques （IR, UV-Vis, and ESR）. In all complexes, the ligand bonds to the metal ion through the azomethine nitrogen and a-hydroxy oxygen atoms. The structures of Pd（Ⅱ） complex 8 and Ru（Ⅲ） complex 9 were found to be polynuclear. Two kinds of stereochemical geometries; distorted tetrahedral and distorted square py- ramidal, have been realized for the Cu（Ⅱ） complexes based on the results of UV-Vis, magnetic susceptibility and ESR spectra whereas octahedral geometry was predicted for Co（Ⅱ）, Mn（Ⅱ） and Ru（Ⅲ） complexes. Ni（Ⅱ） com- plexes were predicted to be square planar and tetrahedral and Pd（Ⅱ） complexes were found to be square planar. The antimicrobial activity of the ligands and their metal complexes was also investigated against the gram-positive bac- teria Staphylococcus aures and Bacillus subtilis and gram-negative bacteria, Escherichia coli and Pesudomonas aeruginosa, by using the agar dilution method. Chloramphenicol was used as standard compound. The obtained data revealed that the metal complexes are more or less, active than the parent ligand and standard. The X-ray crys- tal structure of HL3 has been also reported.

云南省玉米白斑病病原菌鉴定与品种田间抗性评价

为明确近年来云南省严重发生的玉米白斑病病原菌及不同玉米品种对玉米白斑病的抗性差异,2021年于云南省昆明市阿子营乡和曲靖市罗平县九龙镇采集病样,进行病斑组织分离纯化,通过形态学特征和分子生物学方法对病原菌进行鉴定,并在云南省曲靖市罗平县九龙镇采用田间自然发病方法对622份玉米品种的抗性进行鉴定。从2个采样地点的病叶中各分离出5株和9株菌株,经柯赫氏法则验证,确定了菌株WS-1、WS-191为白斑病致病菌;经形态学观察以及基于ITS、rpb2、tub2和LSU基因序列构建的系统发育树分析结果确定其分类学种名为宽颈附球菌Epicoccum latusicollum。田间抗病性试验结果表明,在622份区域试验品种中对白斑病表现高抗、抗病、中抗、感病和高感的品种分别为4、184、216、186和32份,分别占品种总数的0.64%、29.58%、34.73%、29.90%和5.14%。