Bone marrow-derived mesenchymal stem cell-derived exosomeloaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.

3D obstacle detection of indoor mobile robots by floor detection and rejection

Obstacle detection is essential for mobile robots to avoid collision with obstacles.Mobile robots usually operate in indoor environments,where they encounter various kinds of obstacles;however,2D range sensor can sense obstacles only in 2D plane.In contrast,by using 3D range sensor,it is possible to detect ground and aerial obstacles that 2D range sensor cannot sense.In this paper,we present a 3D obstacle detection method that will help overcome the limitations of 2D range sensor with regard to obstacle detection.The indoor environment typically consists of a flat floor.The position of the floor can be determined by estimating the plane using the least squares method.Having determined the position of the floor,the points of obstacles can be known by rejecting the points of the floor.In the experimental section,we show the results of this approach using a Kinect sensor.

MiR-142-3p Regulates ILC1s by Targeting HMGB1 via the NF-κB Pathway in a Mouse Model of Early Pregnancy Loss

Objective Innate lymphoid cells(ILCs)are a class of newly discovered immunocytes.Group 1 ILCs(ILC1s)are identified in the decidua of humans and mice.High mobility group box 1(HMGB1)is predicted to be one of the target genes of miR-142-3p,which is closely related to pregnancy-related diseases.Furthermore,miR-142-3p and HMGB1 are involved in regulating the NF-κB signaling pathway.This study aimed to examine the regulatory effect of miR-142-3p on ILC1s and the underlying mechanism involving HMGB1 and the NF-κB signaling pathway.Methods Mouse models of normal pregnancy and abortion were constructed,and the alterations of ILC1s,miR-142-3p,ILC1 transcription factor(T-bet),and pro-inflammatory cytokines of ILC1s(TNF-α,IFN-γand IL-2)were detected in mice from different groups.The targeting regulation of HMGB1 by miR-142-3p in ILC1s,and the expression of HMGB1 in normal pregnant mice and abortive mice were investigated.In addition,the regulatory effects of miR-142-3p and HMGB1 on ILC1s were detected in vitro by CCK-8,Annexin-V/PI,ELISA,and RT-PCR,respectively.Furthermore,changes of the NF-κB signaling pathway in ILC1s were examined in the different groups.For the in vivo studies,miR-142-3p-Agomir was injected in the uterus of abortive mice to evaluate the abortion rate and alterations of ILC1s at the maternal-fetal interface,and further detect the expression of HMGB1,pro-inflammatory cytokines,and the NF-κB signaling pathway.Results The number of ILC1s was significantly increased,the level of HMGB1 was significantly upregulated,and that of miR-142-3p was considerably downregulated in the abortive mice as compared with the normal pregnant mice(all P<0.05).In addition,miR-142-3p was found to drastically inhibit the activation of the NF-κB signaling pathway(P<0.05).The number of ILC1s and the levels of pro-inflammatory cytokines were significantly downregulated and the activation of the NF-κB signaling pathway was inhibited in the miR-142-3p Agomir group(all P<0.05).Conclusion miR-142-3p can regulate ILC1s by targeting HMGB1 via the NF-κB signaling pathway,and attenuate the inflammation at the maternal-fetal interface in abortive mice.

胃癌组织高迁移率族蛋白A2与基质金属蛋白酶-9表达与肿瘤侵袭转移的关系及预后意义

目的探讨胃癌组织中高迁移率族蛋白A2(HMGA2)及基质金属蛋白酶-9(MMP-9)表达与肿瘤侵袭和转移能力的关系及预后意义。方法采用免疫组化法(SP法)检测93例胃癌组织、30例正常胃黏膜组织中HMGA2、MMP-9的表达;收集患者临床病历资料,并进行随访。结果 HGMA2蛋白在胃癌组织中阳性表达率分别为明显高于正常胃黏膜(P<0.01);MMP-9蛋白在胃癌组织中阳性表达率明显高于正常胃黏膜(P<0.01)。HMGA2与MMP-9在胃癌组织中的蛋白阳性表达均与胃癌组织的肿瘤浸润深度、肿瘤分化程度、淋巴结转移、TNM分期有关(P<0.05);与患者的性别、年龄、肿瘤直径无关(P>0.05)。相关性分析发现,HMGA2与MMP-9在胃癌组织中的表达情况呈正相关(r=0.317,P<0.01)。经Kaplan-Meier生存分析显示,HMGA2、MMP-9蛋白表达阳性患者的生存率均低于表达阴性患者(P<0.01)。结论 HMGA2、MMP-9与胃癌浸润和转移有关,两者表达具有相互协同作用,对胃癌的侵袭和转移起重要的促进作用。HMGA2、MMP-9是影响预后的危险因素,可能成为胃癌治疗的新靶点。

丙泊酚对急性肺损伤大鼠HMGB-1表达的影响

目的探讨丙泊酚的肺保护作用及其可能作用机制。方法采用尾静脉注射内毒素建立急性肺损伤模型,丙泊酚和锌原卟啉同样经过尾静脉给药。将Wistar大鼠按随机数字表法分为实验对照组(C组)、脂多糖组(L组)、脂多糖+丙泊酚组(LP组)。C组尾静脉注射生理盐水;L组尾静脉注射LPS 7.5mg·kg-1;LP组尾静脉注射LPS 7.5mg·kg-1,同时静脉注射丙泊酚30mg·kg-1。给药前及开始后第3、6和12h测定动脉血氧分压(PaO2),实验结束后观察肺湿/干重(W/D)比值、肺组织病理学检查并进行肺损伤轻重程度评分,同时观察各组大鼠肺组织高迁移率族蛋白1(HMGB-1)含量。结果实验前各组PaO2无明显差异,注射LPS后L组PaO2持续下降,和C组相比其PaO2显著降低(P<0.05)。实验结束后L组和C组相比其W/D比值、肺组织病理学检查评分、HO-1及HMGB-1含量显著增加(P<0.05)。而LP组和L组相比其PaO2显著改善,而W/D比值、肺组织病理学检查评分及HMGB-1含量显著降低(P<0.05)。结论丙泊酚具有肺保护作用,且该作用可能与其抑制HMGB-1过度表达作用相关。

基于LERANS模式的护理干预在先天性内眦赘皮患儿矫治中的应用

目的:研究基于聆听-确立-运用-提升-反馈-强化(LERANS)模式的护理干预在先天性内眦赘皮患儿矫正治疗中的应用效果。方法:选择2020年10月-2021年10月笔者科室收治的174例先天性内眦赘皮并行矫正术的患儿,采用随机抽签法分为常规干预组(87例)和反馈强化组(87例)。常规干预组进行常规健康教育干预;反馈强化组在常规干预组的基础上采用基于LERANS模式的护理干预。干预后,比较两组患者眼部疼痛、肿胀程度评分、内眦间距(Innercanthicdiameter,ICD)缩短率、睑裂长度(Lengthofpalpebralfissure,HLFL)/ICD达标率、瘢痕评分、家长对患儿眼部美观的满意度及护理质量评分。结果:术后3 d,反馈强化组疼痛、肿胀程度评分均低于常规干预组(P<0.05),两组内眦间距缩短率及(睑裂长度/内眦间距)达标率比较差异无统计学意义(P>0.05);干预后,反馈强化组VSS评分低于常规干预组(P<0.05),家长对患儿眼部美观的总满意率及护理质量评分高于常规干预组(P<0.05),总并发症发生率低于常规干预组(P<0.05)。结论:采用基于LERANS模式的护理干预可有效缓解先天性内眦赘皮患儿术后疼痛、肿胀程度,减少并发症,减少瘢痕形成,提高患儿家长对患儿眼部美观的满意度,具有较好的干预效果。

Influence of Ⅴ/Ⅲ ratio on the structural and photoluminescence properties of In_(0.52) AlAs/In_(0.53) GaAs metamorphic high electron mobility transistor grown by molecular beam epitaxy

A series of metamorphic high electron mobility transistors （MMHEMTs） with different Ⅴ/Ⅲ flux ratios are grown on CaAs （001） substrates by molecular beam epitaxy （MBE）. The samples are analysed by using atomic force microscopy （AFM）, Hall measurement, and low temperature photoluminescence （PL）. The optimum Ⅴ/Ⅲ ratio in a range from 15 to 60 for the growth of MMHEMTs is found to be around 40. At this ratio, the root mean square （RMS） roughness of the material is only 2.02 nm; a room-temperature mobility and a sheet electron density are obtained to be 10610.0cm^2/（V.s） and 3.26×10^12cm^-2 respectively. These results are equivalent to those obtained for the same structure grown on InP substrate. There are two peaks in the PL spectrum of the structure, corresponding to two sub-energy levels of the In0.53Ga0.47As quantum well. It is found that the photoluminescence intensities of the two peaks vary with the Ⅴ/Ⅲ ratio, for which the reasons are discussed.

Formation permeability evaluation and productivity prediction based on mobility from pressure measurement while drilling

Based on the measurement mechanism of mobility in pressure measurement while drilling, through analyzing a large number of mobility data, it is found that under the condition of water-based mud drilling, the product of mobility from pressure measurement while drilling and the viscosity of mud filtrate is infinitely close to the water phase permeability under the residual oil in relative permeability experiment. Based on this, a method converting the mobility from pressure measurement while drilling to core permeability is proposed, and the permeability based on Timur formula has been established. Application of this method in Penglai 19-9 oilfield of Bohai Sea shows:(1) Compared with the permeability calculated by the model of adjacent oilfields, the permeability calculated by this model is more consistent with the permeability calculated by core analysis.(2) Based on the new model, the correlation between the calculated mobility of well logging and the actual drilling specific productivity index bas been established. Compared with the relationship established by using the permeability model of an adjacent oilfield, the correlation of the new model is better.(3) Productivity of four directional wells was predicted, and the prediction results are in good agreement with the actual production after drilling.

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra- and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.

远程教育成人学习需求与学习支持的新平衡

现代远程教育在经历了一定时期的发展之后,学习者的思想观念由传统教育模式向远程教育模式逐渐转变,随之,学习者学习支持服务的需求日趋多样化、个性化和效率化。而远程教育支持服务体系在新形势下更需做出一些必要的调整,以更好地迎合新的学习需求。

合成气制低碳烯烃的路线评述

一、前言 合成气是可替代石油生产燃料油和化工产品的重要中间原料,可由天然气或煤制取,具有良好的开发与应用前景。自20年代德国首创费-托合成法后,许多国家曾致力研究开发,3、40年代在油源匮缺的欧洲曾兴建多座人造石油工厂。二战后,国际石油生产形势有很大变化与起伏,80年代仍有两套大型合成燃油工业装置在南非建成投产。Mobil公司承建的合成气经甲醇制汽油的工业流程也已在新西兰建成投产。我国近年来由中科院山西煤化所牵头的费-托法合成汽、煤油的中间试验厂亦在顺利运转中。

Developing architecture of a traveler information system for dynamic equilibrium in traffic networks

Having criticized the current architecture of Advanced Traveler Information Systems （ATISs）, this work discusses a new base of requirements to develop a new paradigm for traffic information systems. It mainly integrates three dimensions within a traffic system： drivers＇ pattern of behavior and preferences, urban traffic desires, and capabilities of traffic information service providers. Based on the above, functional segments from several related backgrounds are brought together to structure a new architecture, called Interactive Traveler Information System （ITIS）. The main interactive feature of this new architecture is a two-way communication track between drivers and the traffic information system provider-in fact, a decision on choosing a road at a particular time for an individual will be made based on the utility of both sides. This new configuration consists of driver-side smartphone application, centric traffic prediction, and decision-maker units, which will shape a new approach of decision-making processes. These all work together to satisfy the designated goal of ITIS, which is preserving the Wardrop equilibrium condition in the traffic network level. Finally, we concentrate on a comparison study, which shows a differentiation between performance of the proposed ITIS and the current ATIS model in a real situation. This has been done with simulations of analogical scenarios. The most noticeable advantage of the proposed architecture is not being limited to a saturation limit, and the positive effect of increasing system penetration in the performance of the newly introduced information system. In conclusion, new research subjects are suggested to be carried out.

Obstacle recognition using a haptic device

A haptic device is proposed which gives the user feedback information on their location and orientation of the obstacle through the mobile robot that detects the obstacle in an environment where the user cannot see.Mobile robot recognizes the exact position of the obstacle through configuring the nested ultrasonic sensor and giving feedback information to the haptic device.The haptic device consisting of five vibration motors can realize the haptic through the vibration of user's finger using the position information of the obstacle received feedback.In addition,it has high accuracy to recognize the surrounding environment and realizes the various situations with the fuzzy controller and the nested ultrasonic sensors.

Wireless sensing system for environmental humidity and temperature monitoring

Aiming at the actual demand for monitoring environmental information,a wireless sensing system for temperature and relative humidity(RH)monitoring based on radio frequency(RF)technology and mobile network was designed.This paper introduces the architecture of the system.The system uses AVR micro controller unit(MCU),KYL-1020U RF module and SHT71 to complete real-time temperature and humidity monitoring,and uses SIM900A module to realize remote alarming and monitoring with short message system(SMS)through global system for mobile communication(GSM).Experimental results show that the designed system has good stability of measurement and real-time performance,and it can be used in some small temperature and humidity monitoring occasions.

High electron mobility fluorinated indacenodithiophene small molecule acceptors for organic solar cells

Indacenodithiophene(IDT)derivatives are kinds of the most representative and widely used cores of small molecule acceptors(SMAs)in organic solar cells(OSCs).Here we systematically investigate the influence of end-group fluo rination density and position on the photovoltaic properties of the IDT-based SMAs IDIC-nF(n=0,2,4).The absorption edge of IDIC-nF red-shifts with theπ-πstacking and crystallinity improvement,and their electronic energy levels downshift with increasing n.Due to the advantages of J_(sc)and FF as well as acceptable V_(oc),the difluorinated IDIC-2 F acceptor based OSCs achieve the highest power conversion efficiency(PCE)of 13%,better than the OSC devices based on IDIC and IDIC-4 F as acceptors.And the photovoltaic performance of the PTQ10:IDIC-2 F OSCs is insensitive to the active layer thickness:PCE still keep high values of 12.00%and 11.46%for the devices with active layer thickness of 80 and 354 nm,respectively.This work verifies that fine and delicate modulation of the SMAs molecular structure could optimize photovoltaic performance of the corresponding OSCs.Meanwhile,the thickness-insensitivity property of the OSCs has potential for large-scale and printable fabrication technology.

Investigation on two triphenylene based electron transport materials

Promoting electron mobility is the key to designing high performance electron transport materials(ETMs). Formation of intermolecular interaction can be helpful to enhance their electron mobilities as a result of more ordered molecular stacking.Here, to reveal the inherent influence of intermolecular π-π stacking on the electron mobilities, we designed two ETMs, namely,2,4-diphenyl-6-[3-(2-triphenylenyl)phenyl]-1,3,5-triazine(TPTRZ) and 2,4-diphenyl-6-[4′-(2-triphenylenyl)[1,1′-biphenyl]-3-yl]-1,3,5-triazine(TPPTRZ). Thermal, photophysical and electrochemical measurement results indicate they are good ETM candidates. Additionally, TPTRZ and TPPTRZ exhibit high electron mobilities of 3.60×10^(-5) and 3.58×10^(-5) cm^2V-1 s^(-1), respectively, at an electric field of 7×10~5 V cm^(-1). By taking X-ray single crystal structure, theoretical calculation and time of flight(TOF) results into consideration, it is revealed that strong intermolecular π-π stacking induced by planar triphenylene and triphenyltriazine units renders TPTRZ and TPPTRZ small energetic and positional disorder parameters, and results in their high electron mobilities thereby. By further enhancing intermolecular π-π stacking, ETMs with even higher electron mobilities can thus be anticipated.

专利检索

<正>专利名称:低黏度润滑油基础油的制备工艺专利权人:Luo;Shuji;Patil;Abhimanyu Onkar(Exxon Mobil Research and Engineering Company)专利号:US 8,889,931公开日期:2014年11月18日摘要:本专利提供了C6~C24α-烯烃聚合体的制备工艺,这种α-烯烃聚合体组成中包含了至少50%(物质的量浓度)的三聚体α-烯烃。

专利检索

<正>专利名称:饱和烃的制备工艺及其使用专利权人:Wang,等(Exxon Mobil Research and Engineering Company)专利号:US 8,957,266公开日期:2015年2月17日摘要:本专利提供了制备一种或多种不饱和聚酸的工艺技术,同时该工艺技术也可以用于制备一个或多个饱和烃。在其任何一种方式中,用于获得一个或多个饱和烃的工艺技术包括在分子筛催化剂存在下,对碳原子数为4~38的一个或多个不饱和羧酸齐聚,以形成一个或多个不饱和齐聚酸。