Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between pr...Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)storage.Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed significantly.Total sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation.The Fresh(FS),MAP,and air packaging(AP)groups were set.Total of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS group.The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover.Correlation analysis found that 52 DAPs were correlated with quality traits.Among them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity.These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.展开更多
The effects of different modified atmospheres (air: atm1;100% CO2: atm2;100% N2: atm3;50% CO2/50% N2: atm4) on the textural and sensory characteristics of the ready-to-serve pizza at 7℃ ± 1℃ were investigated. ...The effects of different modified atmospheres (air: atm1;100% CO2: atm2;100% N2: atm3;50% CO2/50% N2: atm4) on the textural and sensory characteristics of the ready-to-serve pizza at 7℃ ± 1℃ were investigated. The values of hardness, gumminess and chewiness were found to be increased during the storage period while cohesiveness and springiness exhibited a decreasing trend. Overall, the 100% CO2 product yielded the best textural and sensorial characteristics followed by 50%CO2/50% N2 and 100% N2 when compared to conventional packed pizza at 7℃ ± 1℃. Carbon dioxide caused microbial inhibition as well. The shelf life of ready-to-serve pizza significantly increased upto 45 days (a 300% increase) for the samples packaged under 100% CO2 (atm2), 50% CO2/50% N2 (atm4) and 100% N2 (atm3), compared to conventional air pack (15 days).展开更多
为进一步分析气调包装结合电子束辐照对冰藏蚌肉品质的影响,该研究设置对照组(CK)、气调包装组(ModifiedAtmosphere Packaging,MAP)、电子束辐照组(ElectronBeamIrradiation,EB)以及气调包装结合电子束辐照组(ModifiedAtmospherePackagi...为进一步分析气调包装结合电子束辐照对冰藏蚌肉品质的影响,该研究设置对照组(CK)、气调包装组(ModifiedAtmosphere Packaging,MAP)、电子束辐照组(ElectronBeamIrradiation,EB)以及气调包装结合电子束辐照组(ModifiedAtmospherePackaging Combined with Electron Beam Irradiation,MAP+EB),通过测定冰藏初始点和货架期终点时蚌肉的质构、色泽、挥发性风味物质和脂肪酸组成指标综合评价蚌肉的品质。结果表明,电子束辐照后蚌肉的硬度、咀嚼性降低,但可延缓微生物繁殖对蚌肉质构的影响。蚌肉色差值在冰藏初始点各组间无显著差异(P>0.05),在货架期终点,受电子束辐照影响,EB组和MAP+EB组的L*值下降,b*值上升。冰藏初始点,CK、MAP、EB和MAP+EB组蚌肉中挥发性风味物质的种类分别为8、17、16和19种,说明气调包装和电子束辐照处理显著提高挥发性风味物质的种类(P<0.05),并且醛类、酮类物质总含量提高,烃类、醇类物质总含量降低,但无显著差异(P>0.05)。各保鲜组蚌肉中脂肪酸相对总含量分别为49.55%、49.64%、49.61%和49.90%,无显著差异(P>0.05)。综上,气调包装结合电子束辐照对冰藏蚌肉的贮藏品质无明显的不良影响,适合于蚌肉的物理保鲜。展开更多
为低成本且高效地保持冷藏华月苹果的品质,分别将成熟的华月苹果采用0.02聚乙烯(PE)、0.03PE、0.04PE、0.06PE、自发气调包装(MAP)+1-甲基环丙烯(1-MCP)缓释剂、0.02PE+1-MCP缓释剂、0.03PE+1-MCP缓释剂、0.04PE+1-MCP缓释剂、0.06PE+1-...为低成本且高效地保持冷藏华月苹果的品质,分别将成熟的华月苹果采用0.02聚乙烯(PE)、0.03PE、0.04PE、0.06PE、自发气调包装(MAP)+1-甲基环丙烯(1-MCP)缓释剂、0.02PE+1-MCP缓释剂、0.03PE+1-MCP缓释剂、0.04PE+1-MCP缓释剂、0.06PE+1-MCP缓释剂扎口处理形成自发气调环境,于温度(0±0.5)℃、相对湿度85%~90%条件下贮藏,并以0.02 mm厚PE保鲜袋冕口为对照(CK)。定期监测保鲜袋内的O_(2)和CO_(2)体积分数,在贮藏45、90、135 d以及货架7 d时测定各处理果实的呼吸强度、乙烯释放速率、内在品质、质地性状等指标,并对果实的内在品质、质地性状进行相关性分析,同时调查果实组织褐变(果皮和果肉)的情况。结果表明,不同厚度保鲜袋内O_(2)和CO_(2)体积分数在第40天时基本达到平衡,不同保鲜袋降低O_(2)浓度,提高CO_(2)浓度水平由高到低依次为0.04PE>0.03PE>0.06PE>0.02PE>0.04PE+1-MCP缓释剂>0.06PE+1-MCP缓释剂>0.03PE+1-MCP缓释剂>0.02PE+1-MCP缓释剂。与其他厚度保鲜袋相比,0.02PE+1-MCP缓释剂和0.03PE+1-MCP缓释剂能够有效抑制果实硬度、可滴定酸含量以及质地性状的下降,降低果皮和果肉褐变发生指数,但对果实维生素C和可溶性固形物含量影响不明显。此外,使用厚度为0.04和0.06 mm的保鲜袋极易使华月苹果发生褐变。综上所述,华月苹果自发气调包装袋厚度不宜超过0.03 mm。既可保持果实内在品质,又可防止果实褐变的最佳组合是0.02 mm PE+1-MCP缓释剂,其次是0.03 mm PE+1-MCP缓释剂。本研究为华月苹果自发气调包装生产、应用和商业冷链提供了理论依据和技术支持。展开更多
基金supported by Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD74,2023TD78)the Earmarked Fund for CARS-47(CARS-47)+2 种基金Guangdong Provincial Science and Technology Plan Project(2023B0202010015)Central Public-Interest Scientific Institution Basal Research Fund,CAFS(Sanya Yazhou Bay Science and Technology City(SKJC-2020-02-013))Special Funds for Promoting Economic Development in Guangdong Province(For Modern Fishery)(YueNong 2019B14).
文摘Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage.Tandem mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)storage.Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed significantly.Total sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation.The Fresh(FS),MAP,and air packaging(AP)groups were set.Total of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS group.The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover.Correlation analysis found that 52 DAPs were correlated with quality traits.Among them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity.These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.
文摘The effects of different modified atmospheres (air: atm1;100% CO2: atm2;100% N2: atm3;50% CO2/50% N2: atm4) on the textural and sensory characteristics of the ready-to-serve pizza at 7℃ ± 1℃ were investigated. The values of hardness, gumminess and chewiness were found to be increased during the storage period while cohesiveness and springiness exhibited a decreasing trend. Overall, the 100% CO2 product yielded the best textural and sensorial characteristics followed by 50%CO2/50% N2 and 100% N2 when compared to conventional packed pizza at 7℃ ± 1℃. Carbon dioxide caused microbial inhibition as well. The shelf life of ready-to-serve pizza significantly increased upto 45 days (a 300% increase) for the samples packaged under 100% CO2 (atm2), 50% CO2/50% N2 (atm4) and 100% N2 (atm3), compared to conventional air pack (15 days).
文摘为进一步分析气调包装结合电子束辐照对冰藏蚌肉品质的影响,该研究设置对照组(CK)、气调包装组(ModifiedAtmosphere Packaging,MAP)、电子束辐照组(ElectronBeamIrradiation,EB)以及气调包装结合电子束辐照组(ModifiedAtmospherePackaging Combined with Electron Beam Irradiation,MAP+EB),通过测定冰藏初始点和货架期终点时蚌肉的质构、色泽、挥发性风味物质和脂肪酸组成指标综合评价蚌肉的品质。结果表明,电子束辐照后蚌肉的硬度、咀嚼性降低,但可延缓微生物繁殖对蚌肉质构的影响。蚌肉色差值在冰藏初始点各组间无显著差异(P>0.05),在货架期终点,受电子束辐照影响,EB组和MAP+EB组的L*值下降,b*值上升。冰藏初始点,CK、MAP、EB和MAP+EB组蚌肉中挥发性风味物质的种类分别为8、17、16和19种,说明气调包装和电子束辐照处理显著提高挥发性风味物质的种类(P<0.05),并且醛类、酮类物质总含量提高,烃类、醇类物质总含量降低,但无显著差异(P>0.05)。各保鲜组蚌肉中脂肪酸相对总含量分别为49.55%、49.64%、49.61%和49.90%,无显著差异(P>0.05)。综上,气调包装结合电子束辐照对冰藏蚌肉的贮藏品质无明显的不良影响,适合于蚌肉的物理保鲜。
文摘为低成本且高效地保持冷藏华月苹果的品质,分别将成熟的华月苹果采用0.02聚乙烯(PE)、0.03PE、0.04PE、0.06PE、自发气调包装(MAP)+1-甲基环丙烯(1-MCP)缓释剂、0.02PE+1-MCP缓释剂、0.03PE+1-MCP缓释剂、0.04PE+1-MCP缓释剂、0.06PE+1-MCP缓释剂扎口处理形成自发气调环境,于温度(0±0.5)℃、相对湿度85%~90%条件下贮藏,并以0.02 mm厚PE保鲜袋冕口为对照(CK)。定期监测保鲜袋内的O_(2)和CO_(2)体积分数,在贮藏45、90、135 d以及货架7 d时测定各处理果实的呼吸强度、乙烯释放速率、内在品质、质地性状等指标,并对果实的内在品质、质地性状进行相关性分析,同时调查果实组织褐变(果皮和果肉)的情况。结果表明,不同厚度保鲜袋内O_(2)和CO_(2)体积分数在第40天时基本达到平衡,不同保鲜袋降低O_(2)浓度,提高CO_(2)浓度水平由高到低依次为0.04PE>0.03PE>0.06PE>0.02PE>0.04PE+1-MCP缓释剂>0.06PE+1-MCP缓释剂>0.03PE+1-MCP缓释剂>0.02PE+1-MCP缓释剂。与其他厚度保鲜袋相比,0.02PE+1-MCP缓释剂和0.03PE+1-MCP缓释剂能够有效抑制果实硬度、可滴定酸含量以及质地性状的下降,降低果皮和果肉褐变发生指数,但对果实维生素C和可溶性固形物含量影响不明显。此外,使用厚度为0.04和0.06 mm的保鲜袋极易使华月苹果发生褐变。综上所述,华月苹果自发气调包装袋厚度不宜超过0.03 mm。既可保持果实内在品质,又可防止果实褐变的最佳组合是0.02 mm PE+1-MCP缓释剂,其次是0.03 mm PE+1-MCP缓释剂。本研究为华月苹果自发气调包装生产、应用和商业冷链提供了理论依据和技术支持。
