Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated a...Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.展开更多
[Objectives]This study was conducted to establish a reliable and unique molecular ID for flue-cured tobacco germplasm resources in Hunan Province,further improving the efficiency of germplasm collection and identifica...[Objectives]This study was conducted to establish a reliable and unique molecular ID for flue-cured tobacco germplasm resources in Hunan Province,further improving the efficiency of germplasm collection and identification,and laying a solid material foundation for flue-cured tobacco breeding.[Methods]Twelve pairs of SSR primers with stable amplification and rich polymorphism were screened out from 816 pairs of SSR primers by a step-by-step screening method.As core primers of the SSR core primer library,the polymorphism of SSR primers was analyzed,the genetic relationship of 162 flue-cured tobacco germplasm resources was identified,and the molecular ID cards were constructed.[Results]The result of SSR primer polymorphism analysis showed that a total of 57 alleles were detected by 12 pairs of SSR primers in 165 tobacco germplasm resources,with an average of 4.75 alleles per pair of primers;the average diversity of SSR primers was 0.649;and the average value of Shannon's index was 1.235.The results of cluster analysis showed that 162 flue-cured tobacco germplasm resources were divided into five groups.The members of each group were divided based on genome information,which had nothing to do with their geographical origin.Meanwhile,12 pairs of SSR primers gave each flue-cured tobacco germplasm resource a unique molecular ID code.[Conclusions]From the above results,we can see that the 12 pairs of SSR primers obtained by screening have stable amplification polymorphism,and can serve as the primers of the core primer library,and can be used to construct the unique molecular ID of flue-cured tobacco germplasm resources.展开更多
The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi ...The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources.展开更多
文摘Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.
基金Supported by Changsha Tobacco Company Project(20-24A01)Hubei Tobacco Company Project(027Y2022-011)。
文摘[Objectives]This study was conducted to establish a reliable and unique molecular ID for flue-cured tobacco germplasm resources in Hunan Province,further improving the efficiency of germplasm collection and identification,and laying a solid material foundation for flue-cured tobacco breeding.[Methods]Twelve pairs of SSR primers with stable amplification and rich polymorphism were screened out from 816 pairs of SSR primers by a step-by-step screening method.As core primers of the SSR core primer library,the polymorphism of SSR primers was analyzed,the genetic relationship of 162 flue-cured tobacco germplasm resources was identified,and the molecular ID cards were constructed.[Results]The result of SSR primer polymorphism analysis showed that a total of 57 alleles were detected by 12 pairs of SSR primers in 165 tobacco germplasm resources,with an average of 4.75 alleles per pair of primers;the average diversity of SSR primers was 0.649;and the average value of Shannon's index was 1.235.The results of cluster analysis showed that 162 flue-cured tobacco germplasm resources were divided into five groups.The members of each group were divided based on genome information,which had nothing to do with their geographical origin.Meanwhile,12 pairs of SSR primers gave each flue-cured tobacco germplasm resource a unique molecular ID code.[Conclusions]From the above results,we can see that the 12 pairs of SSR primers obtained by screening have stable amplification polymorphism,and can serve as the primers of the core primer library,and can be used to construct the unique molecular ID of flue-cured tobacco germplasm resources.
基金supported by the Development of Novel Elite Soybean Cultivars and Lines with High Oil Content (No. Z161100000916005-06)the Crop Germplasm Resources Protection Program (Nos. 2014NWB030, 2015NWB030-05)+2 种基金the Platform of National Crop Germplasm Resources of China (Nos. 2014-004, 2015-004)the National Key Technology R&D Program (No. 2011BAD35B06-2-9)the Agricultural Science and Technology Innovation Program (ASTIP) of CAAS
文摘The development of a core set of SNP molecular markers that could be widely used in soybean genetic research would greatly facilitate research into the genetic diversity of soybean.We conducted an analysis of Tokachi nagaha and 137 of its descendant soybean cultivars using 4044 SNP markers with the goal of determining the appropriate number of single-nucleotide polymorphisms(SNPs)needed to construct unambiguous molecular IDs and characterize genetic diversity based on a genetic distance matrix correlation method.When the number of SNPs was held constant,the number of accession pairs that could be distinguished increased as the polymorphism informative content(PIC)value of the SNPs increased.A core panel of 20 selected SNPs from 11 linkage groups with a mean PIC value of 0.3703 and a range of 0.3640–0.3749 was able to identify almost all of the accession pairs in our study[9445 pairs(99.92%)].The eight accession pairs that could not be identified with this core SNP set all originated from the same province and some of them had the same parental cultivars.The molecular IDs of the 138 accessions were constructed using the core 20 SNPs.It is known that both the number of SNPs and PIC values should be considered when SNPs are selected for use in the analysis of genetic diversity.In this study,when the PIC value was 0.3460,the correlation coefficient between the genetic distance matrices associated with a panel of 200 SNPs and the total population was>0.800,indicating satisfactory correlation.Our high-accuracy,high-resolution core SNP panel for germplasm fingerprinting and our findings about assessing genetic diversity will likely markedly improve the management and utilization efficiency of soybean germplasm resources.
