A new cucurbitane triterpenoid from Momordica charantia

One new cucurbitane-type triterpenoid saponin, 5β,19-epoxycucurbita-6,23-diene-3β,19,25-triol-3-O-β-D-allopyranoside （1）,named momordicoside P was isolated from the fresh fruits ofMomordica charantia. The structure of the saponin was elucidated by spectral methods, including 2D-NMR spectra.

不同产地苦瓜中苦瓜皂苷的闪式提取及HPLC测定

目的采用闪式提取和固相萃取相结合的方法来快速测定不同产地苦瓜中momordicoside L、kuguaglycoside C、goyaglycoside-d的量。方法样品经过闪式提取和固相萃取处理后,采用HPLC法进行测定。色谱条件为Kromasil C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(57∶43),体积流量1.0 mL/min,检测波长203 nm。结果 Momordicoside L线性范围为0.048～0.48μg,加样回收率为96.93%,RSD为1.95%;kuguaglycoside C线性范围为0.024～0.36μg,加样回收率为97.80%,RSD为1.19%;goyaglycoside-d线性范围为0.006～0.192μg,加样回收率为93.62%,RSD为1.28%。广东、广西、福建3个产地3种成分的量差异较大。结论福建产苦瓜中momordicoside L量最高,广东产苦瓜中kuguaglycoside C和goyaglycoside-d的量均较高。此方法可作为苦瓜药材质量评价的依据。