IN VITRO ANTI-HEPATOMA EFFECTS OF MONOCYTES AND KUPFFER CELLS ISOLATED FROM HEPATOMA PATIENTS AFTER TREATMENT WITH BIOLOGICAL IMMUNE STIMULANTS

Monocytes (MC), lymphocytes (LC) and Kupffer cells (KC) were isolated respectively from blood and surgical liver samples of patients suffering from he-patocellular carcinoma (HCC). 13 patients were given BCG, mixed bacterium vaccine (MBV) and human white blood cell interferon (IFN), the other 3 patients were not treated with any biological immune stimulants (BIS) and served as controls. The cytosta-tic and cytotoxic effects of MC and KC on human hepatoma SMMC-7721 (TC) were assayed in vitro and the numbers of T total (Tt), T helper (Th) and T suppressor (Ts) cells were counted using CD monoclonal antibody immunofluorescence. The results were as follows: (1) On the 7th day after the first administration of BIS, the cytostatic and cytotoxic effects of MC on TC showed obvious increase over pre-administration. The activity of BIS was 1 ?5 times as high as that in the controls. (2) After 3 administrations, the cytostatic effect of MC on TC increased to the normal level (84%), while the controls remained as before (45%). (3) On the 7th day after first administration, cytostatic and cytotoxic effects of KC on TC were 0.5 and 1 times higher respectively than those of the controls. (4) The numbers of Tt and Th of patients given BIS increased continuously; on the contrary Ts decreased in number. These results indicate that combined use of BCG, MBV and IFN can actively enhance the immune anti-hepatoma function of patients suffering from HCC.

Expression of ICAT and Wnt signaling-related proteins in the monocytic differentiation of HL-60 cells induced by a new steroidal drug NSC67657

Objective To investigate the expression of mRNA and proteins ofβ-catenin,TCF-4(ICAT)and Wnt signaling pathway-related genes in the monocytic differentiation of acute myeloid leukemia HL-60 cells induced by a new steroidal drug NSC67657.Methods Wright’s staining andα-NBE staining were used to observe the differentiation of HL-60 cells after 5 days of

OxLDL upregulates growth-regulation oncogene α expression in human endothelial cells

Objective To explore the effect of oxLDL on CXC chemokine growth-regulated oncogene α (GROα) expression in human endothelial cells and the possible functional significance of the effect.Methods LDL was isolated by sequential ultracentrifugation and oxidized to oxLDL. Reverse transcription-polymerase chain reaction with GAPDH as internal standard was applied and CXC chemokine GROα mRNA in endothelial ECV304 cells was examined. ELISA was used to determine GROα protein expression on ECV304 cell surface and in the medium. With static cell adhesion assays, the physiological significance of elevated GROα expression was tested. Results OxLDL, not LDL, treatment of ECV304 cells significantly induced the expression of GROα mRNA that was not detectable in untreated cells. Induction of expression was first evident at 1?h, became maximal at 2?h, and was substantially decreased by 4?h. In a concentration- and time-dependent manner, oxLDL, and not LDL, induced a significant upregulation of GROα surface expression in ECV304 cells that was at a barely detectable level in unstimulated ECV304 cells. GROα protein in the medium did not change significantly. Exposure of ECV304 cells to 40?μg protein /ml oxLDL for 24?h resulted in a marked increase in the number of U937 cells bound to ECV304 cells and antibodies to GROα inhibited adhesion.Conclusion OxLDL functionally upregulated GROα expression in endothelial cells.

Human monocyte-derived dendritic cells expressing both chemotactic cytokines IL-8, MCP-1, RANTES and their receptors, and their selective migration to these chemokines

To characterize the mRNA expression of CXC chemokine IL 8, CC chemokine monocyte chemothractant protein 1 (MCP 1) and regulated on activation, normal T cell expressed and secreted (RANTES), and a newly defined DC chemokine DC CK1 as well as the expression of IL 8 receptor, MCP 1 receptor and RANTES receptor in human monocyte derived dendritic cells (MoDCs) The migratory responsiveness of MoDC to IL 8, MCP 1 and RANTES was also studied Methods In vitro generated MoDCs were obtained by differentiating monocytes in the presence of GM CSF and IL 4 for 5 days The time course of RNA expression was analyzed by RT PCR and migratory ability was assessed by a micromultiwell chemotaxis chamber assay Results IL 8, MCP 1, RANTES and their corres^ponding receptors were consistently expressed in MoDCs DC CK 1 expression was detectable after 48 hours of differentiation MoDC selectively migrated in response to MCP 1 and RANTES but not to IL 8 though transcripts of IL 8 receptor were present Conclusion Because the capacity of dendritic cells to initiate immune responses depends on their specialized migratory and tissue homing properties, the expression of chemokines and their receptors along with the migratory responsiveness to chemokines of MoDC in our study suggests a potential role of chemokines in the interaction between dendritic cells and T cells and the induction of immune responses

Effects of Wenxiao Ⅱ Decoction on the expression of MCP-1 and VCAM-1 in atherosclerotic rabbits

OBJECTIVE:To observe the effects of different doses of Wenxiao Ⅱ Decoction on the expression of monocyte chemoattractant protein-1(MCP-1) and vascular cell adhesion molecule-1(VCAM-1) in an experimental model of atherosclerosis in rabbits and to explore the mechanism by which it alleviates atherosclerosis.METHODS:Sixty 3-4 month-old New Zealand rabbits of both sexes were randomly divided into six groups:simvastain;model;blank;and high-dose,mid-dose,and low-dose Wenxiao Ⅱ Decoction groups.Except for those in the blank group,all rabbits were fed a high-cholesterol diet.Carotid atherosclerosis was established by balloon-induced injury to the endothelium of the carotid artery in conjunction with consumption of a high-cholesterol diet.After 8 weeks,all rabbits were killed to evaluate the expression of MCP-1 and VCAM-1 by immunohistochemical staining.RESULTS:Expressions of MCP-1 and VCAM-1 were significantly decreased in all groups except the blank group compared with the model group(P<0.05).When compared with the simvastain group only variation of MCP-1 expression in low-dose group was not appreciable,and the differences were indistinct(P<0.05).When comparing among Wenxiao Ⅱ Decoction groups,MCP-1 expression in the mid-and high-dose groups was significantly lower than that seen in the low-dose group(P<0.01),but there were no differences among three dosage groups with respect to VCAM-1 expression(P>0.05).CONCLUSION:These data suggested that high,mid,and low doses of Wenxiao Ⅱ Decoction can inhibit the expression of MCP-1 and VCAM-1,which may prevent the formation of or stabilize atherosclerotic plaques.There may be a direct relationship between the dosage of Wenxiao Ⅱ Decoction and its therapeutic efficacy.