Extended protective effects of three dimensional cultured human mesenchymal stromal cells in a neuroinflammation model

BACKGROUND Human mesenchymal stromal cells(MSCs)possess regenerative potential due to pluripotency and paracrine functions.However,their stemness and immunomod-ulatory capabilities are sub-optimal in conventional two-dimensional(2D)culture.AIM To enhance the efficiency and therapeutic efficacy of MSCs,an in vivo-like 3D culture condition was applied.METHODS MSCs were cultured on polystyrene(2D)or in a gellan gum-based 3D system.In vitro,prostaglandin-endoperoxide synthase 2,indoleamine-2,3-dioxygenase,heme oxygenase 1,and prostaglandin E synthase gene expression was quantified by quantitative real-time polymerase chain reaction.MSCs were incubated with lipopolysaccharide(LPS)-treated mouse splenocytes,and prostaglandin E2 and tumor necrosis factor-alpha levels were measured by enzyme linked immuno-sorbent assay.In vivo,LPS was injected into the lateral ventricle of mouse brain,and MSCs were administered intravenously the next day.Animals were sacrificed and analyzed on days 2 and 6.RESULTS Gellan gum polymer-based 3D culture significantly increased expression of octamer-binding transcription factor 4 and Nanog homeobox stemness markers in human MSCs compared to 2D culture.This 3D environment also heightened expression of cyclooxygenase-2 and heme-oxygenase 1,enzymes known for immunomodulatory functions,including production of prostaglandins and heme degradation,respectively.MSCs in 3D culture secreted more prostaglandin E2 and effectively suppressed tumor necrosis factor-alpha release from LPS-stimulated splenocytes and surpassed the efficiency of MSCs cultured in 2D.In a murine neuroinflammation model,intravenous injection of 3D-cultured MSCs significantly reduced ionized calcium-binding adaptor molecule 1 and glial fibrillary acidic protein expression,mitigating chronic inflammation more effectively than 2D-cultured MSCs.CONCLUSION The microenvironment established in 3D culture serves as an in vivo mimetic,enhancing the immunomodulatory function of MSCs.This suggests that engineered MSCs hold significant promise a potent tool for cell therapy.

“Culture time”板块教学中跨文化意识培养的策略

在新课程改革的大环境中,文化意识成为英语学科核心素养的重要组成部分。语言是文化的载体,语言与文化是相互依赖、相互影响、相互制约的。学习语言的过程也是学习文化的过程。文章主要结合译林版英语五、六年级教材中的Culture time板块的教学案例,探讨如何在英语课堂教学实践中丰富文化素材、发掘其中的文化元素和领略中西方文化异同,以帮助学生有效学习文化知识,拓宽文化视野,培养文化意识。

Research on the Influence of Network Culture on the Moral Sense of College Students

With the rapid development of information technology,the network,as"the fourth media"that college students contact most,has significant impact on the view of life,values and view of the world of college students. Various functions of the network culture also play an important role in the process of moral socialization of college students and in the change of moral cognition,moral emotion and moral will of college students. Thus,we should take full advantage of the multi-function of the network culture,spreading social mainstream values,holding various network moral education activities so as to improve the level of moral sense of college students. This paper attempts to research the influence of network culture from the perspective of moral mentality,starting from the functions of network culture and analyzing the influence of network upon the moral sense of college students,based on which this paper puts forward new concepts and methods of improving the moral education of college students.

Research on Developing Traditional Chinese Martial Arts Culture and Effectively Penetrating Moral Education

In order to implement the requirement of Minislry of Education, ＂further deepen the reform of moral education courses, and play a role in the classroom main channel＂, with literature analysis, case studies and other methods, we make an objective analysis of the real issues of ＂sports disciplines teaching moral education ＂. The results show that physical teachers should effectively improve the penetration ideology and moral education wisdom and teaching skills; they should grasp the connection between moral and discipline and integrate ＂moral goals＂ organically into a three-dimensional target subjects, and implemented it in accordance with the corresponding dimension specific content; in the implementation process, we should focus on using a variety of learning styles, making clever use of resources and make the best use; we need to persevere, to strengthen the sports discipline of moral norms infiltration operations, and better protect the effective penetration of Physical Education in Moral Education.

Integration of Ethnic Minority Cultural Moral Education in Ethnic Minority Areas-Taking the Mongolian Culture as an Example

The traditional culture of ethnic minorities embodies the spiritual pursuit of unique national value.It can be used as a valuable moral education resource and is of great significance to improve the effectiveness of moral education in colleges and universities in ethnic areas.At present,China has not paid enough attention to the development and utilization of moral education resources of the ethnic minority traditional culture,nor has it established and improved the relevant development and utilization mechanism.Taking the moral education spirit of the traditional Mongolian culture as an example,this paper analyzes its moral education value,points out the existing problems of moral education in colleges and universities in ethnic areas,as well as suggests strategies for the integration of moral education resources of ethnic minority traditional culture with the moral education in colleges and universities in ethnic areas.

Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough

The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.

Mining the Essence of Chinese Traditional Culture; To Strengthen College Students＇ Ideological and Moral Education

This paper discusses how to excavate the essence of Chinese traditional culture, In contemporary college moral education make some discussion from the historical and cultural educational ideas and way of education, For higher education worker puts forward several ideas.

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

Investigation of the Acceptability of Cultured Meat in University Students

Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative.

The Philosophy of He(和):Examined from the Perspectives of Technology and Culture

Pre-modern Chinese crafts,such as iron smelting,cookery,medicine,and the production of vehicles,bows,and arrows indicate the traditional Chinese view of technology as being organic,holistic,and comprehensive.This view of technology is guided by the concept of he(和)and employs the means and methods of he,thus achieving the purport of he.In Chinese,the character he(和)possesses positive connotations.It originated from the meaning of"to season;to add flavoring to"(调和)and that of flavors being"perfectly harmonious"(和美).From this sensory experience,he gradually extended to the abstract levels of materiality,humanity,sociality,"order"(wei位),and "power,situation,force"(shi势).Finally,he was elevated to the supreme level of"qi of great harmony"(taihe zhi qi太和之气),which is comparable to the concept of dao(道).The philosophy of he has exerted wide impact on such areas as technology,art,national character,cultural psychology,and behavior patterns,and has become an integral part of China's inherent culture.The paradoxes and deviations of he hold their own profound philosophical implications that merit further exploration.As humanity confronts significant challenges,such as how we can coexist with others,with technology,and with nature,the ancient Eastern wisdom embodied in he continues to possess practical characteristics and value.

Effects of Different Culture Conditions and Growth States on the Structure and Hemostatic Properties of Coscinodiscus sp.

As a renewable marine inorganic material,Coscinodiscus sp.has significant potential in the field of rapid hemostasis.However,the low yield of Coscinodiscus sp.seriously limits the application.In this study,two new culture modes were adopted to increase the production of Coscinodiscus sp.,the effect of changes in culture conditions and growth status on the hemostatic activity of diatoms was detected.To prevent Coscinodiscus sp.from sinking in culture,the suspension culture mode was realized by adding0.5%agar.The semi-continuous high nutrient concentration culture mode increased the cell density of Coscinodiscus sp.to 11000cells mL^(-1)and shorten the culture cycle to 5 d.In terms of coagulation activity,the addition of frustules reduced the in vitro coagulation time by half and the activation time of coagulation by 70%.The hemolysis rate and cytotoxicity of frustules harvested in the two culture modes did not change significantly.The results showed that suspension culture mode and high nutrient concentration culture mode only changed the growth state of Coscinodiscus sp.,while the hemostatic performance remained stable.

Current status and challenges for cell-cultured milk technology: a systematic review

Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional agriculture.While most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular agriculture.This review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk production.Cellular agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular agriculture.Currently,various companies synthesize milk components through precision fermentation technology.Nevertheless,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation technology.Hence,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.

Exploring the Path of Implementing the Home-School Co-education Model from the Perspective of Moral Education Field

As a social psychological field derived from the concept of physical field,the moral education field plays a very important role in guiding the construction of a home-school co-education model.In this paper,by analyzing the internal power system of the moral education field,with a policy oriented approach and combined with contemporary factors,it aims to empower the traditional home-school co-education model.Only by combining home education and school education,supplemented by social policy guidance,strengthening the construction of the"trinity"community,creating a harmonious and stable ecological interactive moral education field,expanding the path of home-school cooperation,strengthening the boundary of home and school responsibility,and innovating the form of home-school co-education,can it support the bright future of education.

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

On the Dilemma of Contemporary Liberal Theory of Moral Rights for Penalty Justification——Exemplified by the Right to Personal Liberty

Contemporary liberal theory on moral rights argues that moral rights associated with personal liberty constitute a strong constraint on the boundaries of state power.Therefore,the core issue of the penalty justification is not the purpose of the penalty,but the reason for the penalty to refrain from infringing on the moral rights of individuals.In order to justify the penal system,scholars have explored solutions such as limiting the content of rights,waiving rights,and finally rights forfeiture.However,the concept of rights forfeiture cannot be reasonably integrated into the framework of the liberal theory of moral rights.The failure of these attempts stems from the patchwork understanding of rights presupposed by the liberal theory of moral rights.There is another systematic way of understanding rights that offers a better justification.Individual rights are not an independent non-derivative moral justification,and both individual rights and the penal power of the state are only part of a specific(realistic or ideal)system of rules that collectively serve certain values.The real question of penalty justification is not why the punishment does not infringe on the moral rights of individuals,but whether the overall institutional arrangements,including the penal system,are justifiable for all citizens,including the punished.

Comparative Study of the Widal Test against Stool Culture in the Diagnosis of Suspected Cases of Typhoid Fever in Some Low Income Communities in the Adamawa Region of Cameroon

Introduction: Infectious diseases constitute a major concern of public health in developing countries. Facilities and well trained staff have been shown to be one of the major obstacles in the rapid and quality diagnosis of these diseases. As such, we carried out an analysis to compare the Widal test and stool culture to identify febrile patients with Salmonella infection. Method: A cross sectional study was conducted to diagnose salmonella infection with out-patients who demonstrated signs of salmonella infection. Serum was harvested from blood collected from 368 (Vina = 234, Mayo Banyo 65, and Djerem = 69) patients accompanied by stool, Widal test was conducted on the spot and stool was taken to a reference laboratory for culture using standard microbiological methods, sociological set up was calculated in percentages, prevalence was calculated using excel while statistical difference was calculated using SPSS. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated to compare the Widal test against stool culture. Results: A total of 368 (50.8% females and 49.2% males) participants took part in the survey. Salmonella prevalence (66.24%) in stool culture in the Vina division was significantly different (p 0.05). The sensitivity,specificity, PPV, and NPV of slide agglutination test against stool culture varied from different areas (Vina: 51.6%, 73.62%, 79.21% and 43.61%;Mayo Banyo: 60.53%, 77.78%, 79.31% and 58.33%;Djerem: 53.18%, 83.73% 73.91% and 67.39%) respectively. Slide agglutination test has a fair agreement with the stool culture (kappa, Vina = 0.202;Mayo Banyo = 0.37 and Djerem = 0.38). Conclusion: Generally, in the three areas of study, the Widal test had a fair correlation with the stool culture;This means the Widal test should not be used alone but in combination with stool culture in the detection of salmonella infections.

Historiography and Scientific Research of Religious Buildings of the Cucuteni-Trypillian Culture

The purpose of this article is to reveal the historiography and current state of research related to the cult buildings of the Eneolithic period of the Cucuteni-Trypillian cultural community.The article describes the historical path of archaeological discoveries,from the first Trypillian sanctuaries to the discovery of the largest Nebelivka temple complex in Ukraine in 2012.At the same time,the work partially raises the issue of religious beliefs of the ancient farmers of Central Europe in connection with the discovery of their sacred buildings.Since special scientific works have not comprehensively addressed this issue and are still fragmentary in nature,it is important at the present stage of research to make some coverage and systematization of existing materials on this issue.

Tourism Mode of Tea Culture Museum in Guizhou:A Case Study of Meitan Modern Tea Culture Museum Settlement

Since the 14 th Five-Year Plan,tea culture has become very popular.However,due to the lack of brand awareness and overall planning,and the mechanical application of the model outside the province and other tourism products,Guizhou Tea Culture Tourism presents a"strong tea,light culture,weak tourism".In view of this,in order to realize the positioning goal of"building a national demonstration zone for the integration of tea culture and tourism"in the 14 th Five-Year Plan of Guizhou tea industry,and explore the development mode of integration of tea culture and tourism with Guizhou characteristics,this study takes museum tourism as the breakthrough point,Meitan Tea Museum settlement as the research object,and collects Meitan Tea Museum tourism resources.This paper uses SWOT analysis to evaluate the Meitan Tea Culture Museum Tourism model,and provides some recommendations for the development of tea culture museum tourism in Guizhou Province.

AIGC Related Context: A New Communication Culture For Human

The emergence and rapid development of Artificial Intelligence Generated Content(AIGC)technology is revolutionizing human communication and cultural landscapes.This paper explores the impact of AIGC on society,identifying three distinct cultural paradigms:AIGC Blended Culture,AIGC Based Culture,and AIGC Polluted Culture.AIGC Blended Culture represents a synergistic collaboration between AI and human creativity,enhancing content creation efficiency,promoting cross-cultural communication,and enriching human experiences.AIGC Based Culture signifies a shift where AI becomes the core driver of cultural production and dissemination.This leads to innovative cultural industry models,expanded development fields,and improved cultural product quality and diversity.However,AIGC Polluted Culture arises from the misuse of AIGC to produce or disseminate harmful content.The paper proposes countermeasures and recommendations to maximize the benefits of AIGC while mitigating potential risks.This involves fostering collaboration between AI and humans,focusing on talent cultivation,establishing and improving laws and regulations,and developing solutions to detect and mitigate harmful content.Additionally,promoting responsible innovation and ethical AI development is crucial.In conclusion,AIGC represents a transformative force reshaping human communication and culture.While offering immense opportunities for innovation and efficiency,it also presents challenges that require careful consideration and proactive measures to ensure its positive and sustainable impact on society.

Tourism-Oriented Cultural Product Design: A Case Study of Yami Culture in Lanyu

Due to the prevalence of modern commerce and mainstream culture, many indigenous cultures around the world are rapidly diminishing. These cultures, with their unique primitiveness distinct from modern culture, often attract tourists. This study integrates the indigenous Yami culture of Lanyu Island, Taiwan Region, into innovative designs from the perspective of tourists’ understanding of the local culture. In this way, tourists can experience the harmonious coexistence of the Yami tribe and nature, while these culturally rich products foster deeper experiential and emotional resonance. This study employs the Mandala thinking method for concept expansion and the Osborn checklist method for the analysis and transformation of design elements. Subsequently, designers were invited to conduct on-site travel experiences, leading to the creation of a series of Yami cultural products. Through the evaluation and validation of cultural product assessment indicators, functional commercialized products were ultimately produced. This study aims to establish a design process for cultural products through this design case. It seeks to help users recall their Lanyu Island travel experiences and appreciate the often-overlooked cultural beauty, thereby raising awareness of cultural preservation.