α-Fe_2O_3纳米粒子Morin相变的Raman光谱研究

本文首次用Raman光谱法研究了粒子尺寸和Co2 +包附对α-Fe2 O3纳米粒子Morin相变温度TM的影响机制。测量结果显示纳米粒子α -Fe2 O3的振动模相对大块样品发生了红移和宽化 ,粒子愈小红移和宽化愈显著。表明表面Fe3+离子与配位氧离子的键距增大 ,键长呈从体相Fe-O键长过渡到表面Fe -O键长的某种分布。这种小尺寸引起的键长改变导致α -Fe2 O3粒子的单离子各向异性能减小 ,因此降低了样品的TM。分析显示Co2 +包附导致α -Fe2 O3粒子TM 的大幅下降可能是由单离子各向异性的减小和Co2

超声改良Morin法在婴儿发育性髋关节发育不良筛查中的应用效果研究

目的探讨分析超声改良Morin法在婴儿发育性髋关节发育不良(DDH)筛查中的应用效果。方法选取2016年1月至2019年1月在某院进行婴儿DDH超声筛查的6月龄以内的婴儿1596例(3192侧髋)作为本次研究对象,所有婴儿均采用彩色多普勒超声诊断仪检查,并分别运用Graf超声测量法和改良Morin超声测量法进行诊断筛查。比较两种超声测量法诊断DDH的检出情况,并进行筛查结果的一致性检验。结果Graf超声测量法和改良Morin超声测量法分别检出15例、13例DDH,检出率分别为0.47%、0.41%。Graf超声测量法筛选出Ⅱb、Ⅱc、D、Ⅲ、Ⅳ型分别4侧、4侧、3侧、2侧、2侧。改良Morin超声测量法筛选出髋关节松弛、半脱位、脱位分别为6侧、4侧、3侧。两种超声测量法筛查DDH的Kappa值为0.791,两者的一致性良好。结论在婴儿DDH的临床筛查中,采用改良Morin超声测量法进行诊断,分型标准简单易行,且筛查结果与传统Graf法相当,可作为临床首选的DDH筛查的简便手段。

油酸包裹对不同尺寸纳米α-Fe_2O_3晶体Morin相变影响的Mssbauer研究

用 Mossbauer方法研究了冻油酸中的 35nm与 6 0 nm均匀α- Fe2 O3 微晶的 Morin相变温度 ,发现它比未包裹样品分别高了 50 K和 2 4

Sc(Ⅲ)-Morin-SDS体系荧光性质的研究和应用

十二烷基硫酸钠(SDS)能强烈增敏Sc(Ⅲ)-Morin络合物的荧光,据此提出了一个高灵敏度测定钪的荧光分析法:线性范围为1.4ng/mL～0.2μg/mL,检测下限为1.4ng/mL。用于岩矿中痕量钪的测定,结果良好。SDS同时对该体系的光度性质有增敏作用。无论增敏光度还是增敏荧光,均不改变络合物的组成比,增敏作用主要是由于胶束特殊环境而引起的。

Morin enhances hepatic Nrf2 expression in a liver fibrosis rat model

AIM To investigate whether morin can reduce hepatic fibrosis by activating the NF-E2-related factor 2(Nrf2) signaling pathway.METHODS Twenty male Sprague-Dawley rats were randomly divided into four groups: control group, morin group, carbon tetrachloride(CCl4) group, and morin + CCl4 group. Rats in both the CCl4 and morin + CCl4 groups were injected intraperitoneally with CCl4 at a dose of 2 mL/kg twice a week. Rats in both the morin and morin + CCl4 groups were treated orally with morin at a dose of 50 mg/kg twice a week. Control rats were treated with vehicle only twice a week. At the end-point of the 8 wk of the experimental period, serum AST, ALT, and ALP were measured, and the liver specimenswere obtained for pathological assessment. Real-time PCR and Western blot methods were used to analyze the expression of α-smooth muscle actin(α-SMA), collagen Ⅰ, collagen Ⅲ, Nrf2, heme oxygenase(HO-1), and quinone oxidoreductase 1(NQO1) using frozen liver specimens.RESULTS Morin-treated rats in the morin + CCl4 group had less hyperplasia of fiber tissue, minimal inflammatory cells, and less body weight loss with favorable liver enzyme measurements compared to rats treated with CCl4 only. Additionally, morin-treated rats had significantly lower m RNA and protein expression of α-SMA, collagen Ⅰ, and collagen Ⅲ, but significantly higher m RNA and protein expression of Nrf2, HO-1, and NQO1 compared to rats treated with CCl4 only(P < 0.05).CONCLUSION Morin could play a protective role by inducing the expression of Nrf2 and its downstream antioxidant factors(HO-1 and NQO1) and reducing the expression of α-SMA, collagen Ⅰ, and collagen Ⅲ in CCl4-induced liver fibrosis rats.

Effect of morin, a flavonoid against DOCA-salt hypertensive rats:a dose dependent study

Objective:To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate(DOCA)-salt induced hypertension in male Wistar rats.Methods:Hypertension was induced in uninephrectomized rats by weekly twice subcutaneous injection of DOCA(25 mg/kg bw) and 1% NaCl in the drinking water for six consecutive weeks. Effect of morin against DOCA-salt induced hypertension was evaluated by measuring blood pressure and performing biochemical estimations and histopathological examination of renal tissues.Results:DOCA-salt hypertensive rats showed considerably increased systolic and diastolic blood pressure,serum hepatic marker enzyme activities such as aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP) and gamma-glutamyl transpeptidase(GGT)and renal function markers(urea, uric acid and creatinine) in plasma. Oral administration of morin(25, 50 and 75 mg/kg bw) brought back all the above parameters to near normal level.Histopathology of kidney also confirmed the biochemical findings of this study. The effect at a dose of 50 mg/kg bw of morin was more pronounced than that of the other two doses(25 and 75 mg/kg bw).Conclusions:These findings indicate that morin exhibits strong antihypertensive effect against DOCA-salt induced hypertension.

Anti-epileptic effect of morin against experimental pentylenetetrazol-induced seizures via modulating brain monoamines and oxidative stress

Objective: To evaluate the protective effect of morin against pentylenetetrazol(PTZ)-induced tonic-clonic convulsions in mice. Methods: Swiss albino mice(18-22 g) was used to induce convulsions by intraperitoneal(i.p.) administration of PTZ(90 mg/kg). Mice were either pretreated with morin(10, 20 and 40 mg/kg) or vehicle(distilled water, 10 mg/kg) 45 min before PTZ administration. Various behavioral and biochemical parameters were assessed. Results: PTZ administration resulted in significant production(P<0.001) of tonic-clonic conclusion and mortality in mice. PTZ-induced increase in the duration of convulsion, onset of convulsion and mortality was inhibited significantly by morin(20 and 40 mg/kg) administration. The PTZinduced decrease in brain GABA, dopamine and Na+K+ATPase levels and increase in xanthine oxidase activity were inhibited significantly by morin(20 and 40 mg/kg) treatment. The increased levels of malondialdehyde and nitric oxide level were significantly decreased by morin(20 and 40 mg/kg) treatment. Also, reduced levels of superoxide dismutase and glutathione were increased significantly by morin treatment. Conclusions: Results of the present study indicate that morin showed its anti-convulsant effect via modulating the levels of brain GABA, Na^+K^+ATPase, and oxido-nitrosative stress. Thus, morin can be a potential candidate for further clinical evaluations as an anti-epileptic agent.

Standard Molar Enthalpy of Formation of Morin Studied by Rotating-Bomb Combustion Calorimetry

Flavonols are plant nature. Morin and other related pigments that are ubiquitous in plant flavonols have come into recent prominence because of their usefulness as anticancer, antitumor, anti-AIDS, and other important therapeutic activities of significant potency and low systemic toxicity. The heat of combustion of morin （molecular formula, C15H10O7·H2O） in oxygen was measured by a rotating-bomb type combustion calorimeter, the standard molar enthalpy of combustion of morin at T = 298.15 K was determined to be △cH^ m （C15H10O7·H2O, s） = - （5 937.99±2.99） kJ·mol^-1. The derived standard molar enthalpy of the formation of morin in solid powder state at T = 298.15 K, △fH^ m（C15H10O7·H2O, s）, was -（1 682.12 ± 3.58） kJ·mol^1, which provide an accurate data of the stability of morin to the pharmacy and pharmacology.

Morin attenuates L-arginine induced acute pancreatitis in rats by downregulating myeloperoxidase and lipid peroxidation

Objective:To explore the therapeutic role of morin against L-arginine-induced acute pancreatitis in rats.Methods:The group 1 received two intraperitoneal injections of normal saline,and groups 2-4 were given two intraperitoneal injections of L-arginine(250 mg/100 g body weight)at 1 h interval to induce acute pancreatitis.Subsequently,group 2 received no further treatment while groups 3 and 4 were treated with morin(30 mg/kg)and diclofenac sodium(30 mg/kg),respectively.Blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol were measured.Moreover,histopathological study was carried out to investigate the effect of morin treatment on physiology of the pancreas.Results:L-arginine significantly altered the level of blood glucose and serum levels of insulin,α-amylase,malondialdehyde,myeloperoxidase,alanine aminotransferase,aspartate aminotransferase and cholesterol.Treatment with morin or diclofenac sodium significantly improved the levels of these biomarkers.Furthermore,morin showed more significant effect than diclofenac sodium.Histopathological analysis verified that morin protected the pancreas from deleterious effects of L-arginine.Conclusions:Morin plays a protective role against L-arginineinduced acute pancreatitis via reducing lipid peroxidation and tissue inflammation,and attenuating acute pancreatitis-associated alteration in insulin secretion and glucose metabolism.

Synthesis and Characterization of Fe₃O₄Coated on APTES as Carriers for Morin-Anticancer Drug

Morin (MR) is an anticancer drug present in fruits and Chinese herbs. Fe3O4 magnetic nanoparticles (MNPs) coated on 3-aminopropyl triethoxysilane (APTES) were synthesized (MNPs-APTES) as carriers for MR. The characterization of drug delivery system was confirmed by Fourier Transform Infrared (FTIR), Transmission Electron Microscope (TEM), X-Ray Diffraction (XRD), dynamic light scattering (DLS), and vibrating sample magnetometer (VSM). The adsorbed APTES on the magnetite surface (MNPs-APTES) was examined by FTIR. The TEM image showed that the average particle size is obtained to be about 26.7 nm for MNPs-APTES. The MR loading and release behavior of MNPs-APTES were studied and the results showed that up to 60% of the adsorbed drug was released within 4 h. In summary, the MNPs-APTES nanocarriers are based on the results, promising for targeted morin drug delivery.

SPECTROFLUORIMETRIC DETERMINATION OF GERMANIUM WITH MORIN IN WATER AND HEALTH DRINK

A highly sensitive and fairly selective spectrofluorimetric method has been developed for the determination of germanium with morin in tap water and health drink. The fluorescent reaction and optimal conditions of germanium with morin in phosphoric acid medium were studied. The detection limits of germanium in tap water and health drink were found to be 0.2 and 0.7μd/L respectively.

Zn(II)-Morin配位吸附波的研究及应用

以桑色素（Morin）为配位体，研究了Zn（Ⅱ）与其形成的配合物在汞电极上的极谱行为和极谱波的性质，确定了Zn（Ⅱ）-Morin配合物的组成。在pH=4．7的0．1mol·L^-1 HAc—NaAc底液中，Zn（Ⅱ）-Morin配合物在2．0次微分极谱图上产生良好的吸附还原波。其峰电位为-1．14V，峰电流与Zn^2＋在7．5×10^-8 ～4．3×10^-6mol·L^-1的浓度范围内呈线性关系，最低检出限为7．5×10^-8mol·L^-1。用于实际样品测定，结果令人满意。

油酸及分子筛对α-Fe_2O_3微晶Morin相变影响的Mssbauer研究

用Mossbauer方法研究了冻油酸中的均匀α-Fe_2O_3微晶的Morin相变和吸附在ZSM-5分子筛上的α-Fe_2O_3微晶小粒子的Morin相变,发现冻油酸中的均匀α-Fe_2O_3微晶的Morin相变温度比在空气中的高50K;吸附在ZSM-5分子筛上的α-Fe_2O_3微晶小粒子的Morin相变温度比在空气中的高了70多K。

桑黄素通过抑制ERK1/2-p38信号通路介导对老年大鼠骨骼保护作用

【目的】探讨桑黄素(SSS)治疗对老年大鼠骨代谢及骨量影响,并阐明其可能的作用机制。【方法】10只3月龄年轻雌性Sprague-Dawley(SD)大鼠和20只24月龄老年雌性SD大鼠随机分为3组,对照组(CON,10只年轻大鼠)、模型组(MOD,10只老年大鼠)和桑黄素组(SSS,10只老年大鼠)。在实验过程中,SSS组每日接受腹腔注射桑黄素(10 mg/kg)治疗。治疗为期12周,待治疗结束后使用Micro-CT、HE染色切片、血清学检测以及蛋白质印迹观察治疗效果以及可能的机制。【结果】治疗12周后,与MOD组相比,SSS组的大鼠骨小梁数量和密度得到明显的改善。SSS组左侧股骨BMD、Conn.D、Tb.N、Tb.Th和Tb.Sp较MOD组明显改善(P<0.05)。治疗12周时,SSS组CTX-1、骨钙素、TRACP-5b和PINP水平较MOD显著降低(P<0.05)。和MOD组比较,SSS组的大鼠ERK1/2-p38信号通路显著抑制,ERK1/2和p38水平显著降低,比较有统计学差异(P<0.05)。【结论】桑黄素通过抑制ERK1/2-p38信号通路和降低骨转换来介导对老年大鼠骨骼保护作用。

α-Fe_2O_3超微粒Morin转变温度的变化

超微粒的磁性明显不同于大晶粒材料,其独特的性质和在众多领域中的应用受到人们的普遍重视。由于尺寸效应,磁有序材料的超微粒不仅具有超顺磁性和集体磁激发现象,其磁转变温度也不同于相同材料的大块样品而受颗粒尺寸的影响。

基于可拓语义与文化基因融合的蒙古族拉弦乐器设计转译研究

目的将文化基因与可拓语义概念相结合,探究蒙古族拉弦乐器的物质存在与隐含内核在现代社会语境中的应用形式和设计方法,弘扬优秀民族文化。方法分析蒙古族拉弦乐器文化基因,得出以造型、纹样、制作技艺为主的显性主导基因,以及其在发展过程融入的环境、工艺内涵等隐性嵌合基因,为可拓基元集组建提供基础;引入可拓学的基元建模方法,建立基于文化基因的基元模型,通过演绎、重构、分解、变异建立显性基因的可拓外观设计元素集,利用图解语义分析,构建隐性基因的可拓风格意象设计元素集,继而进行文化基因的表达与设计创新。结果以马头琴为例,运用可拓语义方法,对显性、隐性文化分别进行重构、图解,并设计应用的设计流程,以验证研究方法的可行性和合理性。结论将蒙古族拉弦乐器具象外观与价值内涵进行可拓语义设计转译,是对蒙古族传统文化数字化保护的探索,也为其他蒙古族乐器文化遗产的特征提取和创新应用提供了参考。

钌表面吸附对α-Fe_2O_3 Morin转变的影响研究

在正常条件下,大块晶态α-Fe_2O_3在温度260K发生反铁磁-弱铁磁转变,自旋方向由晶轴[111]转向(111)平面,即Morin转变.高温(T>260K)下,因反向自旋间存在一夹角而呈弱铁磁性.许多实验研究证明:Mo(?)in转变温度是一结构灵敏量。

现代科技的伦理盲症及其价值规制

现代科技活动奉行自然主义价值观,遵循“价值中立”的客观普遍性原则,致使科学与伦理根源上割裂,表现为科学活动中真理与价值分离、技术应用中人本价值缺失以及科技工作者社会责任意识淡漠,再加之科技工作者团队的封闭性,导致现代科技出现了伦理盲症。理性审视“为知识而知识”的纯粹科技价值观,重塑科学家的社会责任意识,突破传统科技伦理价值限阈,以复杂性整体思维完善科学与伦理良性对话机制,将科学求真与伦理向善联结起来,真正实现伦理价值融入科技决策全过程。

基于mTOR/STAT3信号轴探讨桑色素诱导非小细胞肺癌A549细胞自噬的机制

目的基于mTOR/STAT3信号轴探讨桑色素诱导非小细胞肺癌A549细胞自噬的机制。方法将A549细胞分为空白组及30、60、90、120、150μg·m L^(-1)桑色素组,培养24、48、72 h后采用CCK-8法检测细胞增殖活性,计算细胞抑制率。将A549细胞分为空白组及30、90、150μg·m L^(-1)桑色素组,培养细胞14 d后通过克隆形成实验检测细胞增殖情况;培养细胞24 h后,采用Beyo ClickTMEd U-488检测细胞增殖能力;流式细胞术检测细胞凋亡情况;吖啶橙染色检测细胞自噬情况;透射电镜观察细胞自噬体形成情况;Western Blot法检测细胞中凋亡、自噬及m TOR/STAT3信号轴相关蛋白的表达水平。将A549细胞分为空白组、空白+氯喹(10μg·m L^(-1))组、桑色素(30、150μg·m L^(-1))组、桑色素(30、150μg·m L^(-1))+氯喹(10μg·m L^(-1))组,干预48 h后采用CCK-8法检测细胞活性,计算细胞存活率。结果与空白组比较,干预24 h后60、90、120、150μg·m L^(-1)桑色素组的A549细胞抑制率显著升高(P<0.05,P<0.001);干预48、72 h后桑色素30、60、90、120、150μg·m L^(-1)组的A549细胞抑制率显著升高(P<0.001);30、90、150μg·m L^(-1)桑色素组的A549细胞集落数及绿色荧光的增殖阳性细胞数均显著减少(P<0.01,P<0.001),细胞凋亡率均显著升高(P<0.01,P<0.001),cleaved-PARP蛋白表达水平显著升高(P<0.001);90、150μg·m L^(-1)桑色素组A549细胞的p-P38/P38MAPK蛋白表达水平显著升高(P<0.01,P<0.001);30、90、150μg·m L^(-1)桑色素组A549细胞中出现不同程度的橙黄色荧光,以90、150μg·m L^(-1)桑色素组的橙黄色荧光显著;150μg·m L^(-1)桑色素组A549细胞胞浆中分别出现了自噬体和自噬溶酶体;150μg·m L^(-1)桑色素组A549细胞的LC3-Ⅱ蛋白表达明显上调(P<0.05),90、150μg·m L^(-1)桑色素组A549细胞的Atg16L1-Ⅱ蛋白表达显著上调(P<0.001),p-m TOR/m TOR及p-STAT3/STAT3蛋白表达显著下调(P<0.001)。与桑色素(150μg·m L^(-1))组比较,桑色素(150μg·m L^(-1))+氯喹(10μg·m L^(-1))组的A549细胞存活率明显升高(P<0.05)。结论桑色素能够抑制肺癌A549细胞的增殖,促进其凋亡,并诱导自噬,其作用机制可能与m TOR/STAT3信号轴有关。

The change of Morin transition temperature in nanocrystalline α-Fe_2O_3

THE magnetic property of the ultrafine particles differs from that ot the bulk materials and has a lot of applications. Because of the size effect, magnetically ordered materials exhibit both superparamagnetism and collective magnetic excitation, and the magnetic transition temperature changes in the ultrafine particles with respect to the bulk materials as well. Bulk α-Fe2O3 is weakly ferromagnetic above the Morin transition temperature TM