A morphologically structured model is proposed to describe the batch fermentation of lovastatin according to the growth kinetics of filamentous microorganisms. Three kinds of hyphae are considered in the model: active...A morphologically structured model is proposed to describe the batch fermentation of lovastatin according to the growth kinetics of filamentous microorganisms. Three kinds of hyphae are considered in the model: actively growing hyphae, non-growing hyphae and deactivated hyphae. Furthermore, actively growing hyphae consist of three morphological compartments: apical compartment which gives rise to hyphal tip extension; subapical compartment which is related to hyphal branching; and hyphal compartment which is only responsible for secondary metabolite formation. The kinetics of mycelial growth mechanism is summarized and applied in modeling lovastatin fermentation. A Michaelis-Menten kinetic model with substrate inhibition is proposed for product formation. As expected, the model simulations fit well with experimental data obtained either from a laboratory scale 10L fer-menter or from a pilot-plant scale fermenter.展开更多
The growth of filamentous microorganism is contributed by tip extension and branching. The microscopic growth of filamentous microorganism means the growth process from one or a few spores. In order to describe the mi...The growth of filamentous microorganism is contributed by tip extension and branching. The microscopic growth of filamentous microorganism means the growth process from one or a few spores. In order to describe the microscopic process, a population morphologically structured model is proposed, in which three morphological compartment and their interactions were considered, and the heterogeneity of hyphal growth was included. The model was applied to describe the microscopic growth of Streptomyces tendae and Geotrichum candidum with good agreement. From model prediction, it is concluded that if the number of hyphae is large enough (macroscopic growth), the specific growth rate of filamentous microorganism and the ratio of morphological forms in hyphae will become constant.展开更多
A cellular automata model to simulate penicillin fed-batch fermentation process(CAPFM)was established in this study,based on a morphologically structured dynamic penicillin production model,that is in turn based on th...A cellular automata model to simulate penicillin fed-batch fermentation process(CAPFM)was established in this study,based on a morphologically structured dynamic penicillin production model,that is in turn based on the growth mechanism of penicillin producing microorganisms and the characteristics of penicillin fed-batch fermentation.CAPFM uses the three-dimensional cellular automata as a growth space,and a Moore-type neighborhood as the cellular neighborhood.The transition rules of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes.Every cell of CAPFM represents a single or specific number of penicillin producing microorganisms,and has various state.The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly.展开更多
文摘A morphologically structured model is proposed to describe the batch fermentation of lovastatin according to the growth kinetics of filamentous microorganisms. Three kinds of hyphae are considered in the model: actively growing hyphae, non-growing hyphae and deactivated hyphae. Furthermore, actively growing hyphae consist of three morphological compartments: apical compartment which gives rise to hyphal tip extension; subapical compartment which is related to hyphal branching; and hyphal compartment which is only responsible for secondary metabolite formation. The kinetics of mycelial growth mechanism is summarized and applied in modeling lovastatin fermentation. A Michaelis-Menten kinetic model with substrate inhibition is proposed for product formation. As expected, the model simulations fit well with experimental data obtained either from a laboratory scale 10L fer-menter or from a pilot-plant scale fermenter.
文摘The growth of filamentous microorganism is contributed by tip extension and branching. The microscopic growth of filamentous microorganism means the growth process from one or a few spores. In order to describe the microscopic process, a population morphologically structured model is proposed, in which three morphological compartment and their interactions were considered, and the heterogeneity of hyphal growth was included. The model was applied to describe the microscopic growth of Streptomyces tendae and Geotrichum candidum with good agreement. From model prediction, it is concluded that if the number of hyphae is large enough (macroscopic growth), the specific growth rate of filamentous microorganism and the ratio of morphological forms in hyphae will become constant.
基金supported by the National Natural Science Foundation of China (No.60274060,60375017)the Key Project of Chinese Ministry of Education (No.203002)Scientific Research Common Program of Beijing Municipal Commission of Education (NO.KM200510005026).
文摘A cellular automata model to simulate penicillin fed-batch fermentation process(CAPFM)was established in this study,based on a morphologically structured dynamic penicillin production model,that is in turn based on the growth mechanism of penicillin producing microorganisms and the characteristics of penicillin fed-batch fermentation.CAPFM uses the three-dimensional cellular automata as a growth space,and a Moore-type neighborhood as the cellular neighborhood.The transition rules of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes.Every cell of CAPFM represents a single or specific number of penicillin producing microorganisms,and has various state.The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly.
