Watershed prioritization for conservation planning using RUSLE and morphometric methods,Northwestern Ethiopia

Soil erosion and the subsequent sedimentation are serious environmental problems.Improper land management coupled with intense rainfall has complicated the problem in the Ethiopian highlands.Consequently,soil loss costs a profound amount of the national GDP and deters land productivity.Quantifying soil loss and prioritizing areas for conservation is imperative for proper planning and resource management.Therefore,this study aimed at estimating the mean annual soil loss rate of Gilgel Abay watershed which has critical implications on Lake Tana and Abbay River(Upper Blue Nile River),using RUSLE and morphometric analysis methods.Datasets including rainfall,soil,Digital Elevation Model and land use/land cover were used to generate important parameters required for the soil loss estimation.Data collected through field observation,consultation with experts and document analysis were used for validation purposes.The results of the study reveal that the watershed experienced an average soil loss rate of 39.8 t ha^(-1)yr^(-1).Subsequently,nearly 25%(37,038 ha)of the watershed is threatened by moderate to very severe soil loss.Among the four sub watersheds,sub watershed 3(S3)and sub watershed 4(S4),which consist of about 65%(105,000 ha)of the total area of the study watershed,experienced an annual soil loss rate of over 30 t ha^(-1)yr^(-1).The amount of soil loss that the study watershed has experienced is above the soil loss tolerance level of Ethiopia(2-18 t ha^(-1)yr^(-1))and the tropical region(10 t ha^(-1)yr^(-1)).Similarly,the results of morphometric analysis showed that the studied watershed has S-shaped hypsometric curve,an indication for maturity stage of landscape development.The studied watershed is also characterized by a non-circular shaped drainage with rugged and dissected topography that contributes to high flash flood,peak discharge and incidence of soil loss.The result of both models signified that S3 and S4 are highly susceptible to soil erosion.Therefore,these two sub watersheds need priority for soil and water conservation(SWC)measures.Furthermore,the study demonstrated the importance of the integration of different models in the identification of soil erosion prone areas and prioritization for the proper implementation of SWC measures.

Analysis of Morphometric Parameters and Radioactive Characteristics Using Remote Sensing Data and GIS Techniques in the Wadi Wizr Basin Area, Central Eastern Desert, Egypt

The purpose of this study is to demonstrate how modern technologies such as geographic information systems (GIS) and digital elevation models can help in the creation of a geographic database for the Wadi Wizr basin in Egypt’s Central Eastern Desert, in addition to examining and analysing the radioactive properties of various rocks. This was accomplished with the help of a digital elevation model (DEM) with a 30 metre accuracy and GIS software in 10.8 Arc Map. The RS-230 was also used to measure uranium and thorium concentrations. GIS softwares and digital elevation models have been shown to be more effective than the traditional method. This was demonstrated by the flexible and quick working method, the accuracy of the parameters used, and the results of the morphometric analysis of the basin river network. In addition to, the main drainage pattern from subtype to tree type, where the branching ratio was (1.59). This basin could also cause flooding. Similar studies, according to the results of this study, should make greater use of geographic information system technology and modern data sources. Wadi Wizr also has a radioactive anomaly, with uranium equivalent concentrations reaching 70 ppm in some fault parts.

Evaluation of Morphometric Parameters Studies in Middle-West Part of Kushtia District, Bangladesh, Using Remote Sensing and GIS Techniques

This study finds 1D, 2D and 3D morphometric parameters of the middle-west part of Kushtia district, Bangladesh to implement groundwater potential. For this purpose, the SRTM (DEM) data were used to compute different parameters in GIS environment. The values of 1D parameters like stream order, stream length and mean bifurcation ratio was calculated as 6, 1774.72 km and 2.08 respectively. The computed values of drainage density (0.01 - 23.06 km/km2), stream frequency (1 - 5.64 per km2), drainage texture (0.25 - 0.70 km/km4), length of overland flow (0.02 - 45.05 km2/km), constant of channel maintenance (0.04 - 90.04 km2/km), circularity ratio (0.39 - 0.84), form factor (0.35), elongation ratio (0.62), relief ratio (0.00 - 1.81) and ruggedness number (0.04 - 214.72) disclose the morphometrical conditions of the study area. The results revealed from this study on drainage morphometry can be a great assistance for understanding the hydro-geomorphological character of the area.

Intraspecific leaf morphological variation in Quercus dentata Thunb.:a comparison of traditional and geometric morphometric methods,a pilot study

To compare the application of traditional morphometric methods(TMMs)and geometric morphometric methods(GMMs)in the study of intraspecific leaf morphological characters of Quercus dentata,fifteen linear measurement indices and thirteen landmarks of leaves were used to study leaf morphology of three provenances(H1,H2,and H3).In TMMs,principal component analysis(PCA)showed that leaf size–related indices played an important role in population classification.Partial least square(PLS)analysis showed that the main morphological characters affecting leaf size were the average depth of the lobes and the length–width ratios.However,the important indices to distinguish the provenances were circularity,leaf width,and length–width ratio.The results of discriminant analysis(DA)showed that 74.0%of H1,68.0%of H2,and 74.0%of H3 were correctly classified.Cluster analysis showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 4.3761,11.4629,and 10.2067,respectively.In GMMs,PCA based on symmetrical components showed that the difference in leaf morphology was mainly due to the changing trend of the leaf apex and base,petiole length,and degree of leaf cracking.PLS analysis showed that there was a significant covariation between the leaf symmetrical components and size:as the leaf enlarged,the widest part gradually moved up,and the shape changed from nearly oval to lanceolate.DA results showed that 86.0%and 78.0%of H1 and H2,70.0%and 80.0%of H1 and H3,and 82.0%and 76.0%of H2 and H3 were correctly classified.Canonical variate analysis results showed that the Mahalanobis distances between H1 and H2,H1 and H3,and H2 and H3 were 1.7238,1.5380,and 1.6329,respectively.Both GMMs and TMMs showed significant differences in morphology among the three Q.dentata provenances,but GMMs had higher classification accuracy and could provide more information about leaf shape,whereas TMMs could provide more information about leaf size.Based on our results,GMMs are promising in the study of leaf morphological variation within Q.dentata provenances.

The levels of serum fibrosis marks and morphometric quantitative measurement of hepatic fibrosis

Objective: To study the relationship between the ser- um levels of hyaluronic acid (HA), procollagen type Ⅲ (PCⅢ), collagen type Ⅳ (CIV) and the histologi- cal degree of hepatic fibrosis evaluated by image analysis, and the clinical significance of serum HA, PC Ⅲ, C Ⅳ in the diagnosis of hepatic fibrosis in pa- tients with chronic viral hepatitis. Methods: The concentrations of serum HA, PC Ⅲ, C Ⅳ in 151 patients with chronic viral hepatitis were measured by radioimmunoassay. Liver biopsies were performed in all the patients. Histological sections of 4 μm thickness were stained with Masson's trichrome for fibrosis assessment. Morphometric quantitative measurements for hepatic fibrosis assessment in the 4 μm sections were performed using a fully automated image analysis system. Serum levels of HA, PC Ⅲ, and C Ⅳ were analyzed at different stages of liver pa- thology and compared with the morphometric quanti- tative measurements of hepatic fibrosis. Results: The serum levels of HA, PC Ⅲ, C Ⅳ all ele- vated gradually with the progression of the disease, and all reached the highest in patients with liver cir- rhosis. There was a significant difference in the levels of these 3 components between liver cirrhosis group and the other groups (P<0.05). They all increased steadily with the histological stages of hepatic fibrosis, and reached the highest levels in stage Ⅳ. The serum levels of HA, PC Ⅲ, C Ⅳ were all positive- ly correlated with the histological stages of liver sec- tions and the morphometric measurement (P< 0.001). The coefficients with stages were 0.694, 0.493, 0.552 (P<0.001), respectively and with sur- face density of total collagen on liver biopsy sections by image analysis were 0. 715, 0. 595, 0. 573 (P< 0.001), respectively. Conclusion: The serum levels of HA, PC Ⅲ, C Ⅳ were in consistent with the degree of hepatic fibrosis, and the determination of these marks is valuable for detecting hepatic fibrosis.

Prioritization of Semi-Arid Agricultural Watershed Using Morphometric and Principal Component Analysis, Remote Sensing, and GIS Techniques, the Zerqa River Watershed, Northern Jordan

Remote sensing and GIS techniques were employed for prioritization of the Zerqa River watershed. Forty-three 4th order sub-watersheds were prioritized based on morphometric and Principal Component Analysis (PCA), in order to examine the effectiveness of morphometric parameters in watershed prioritization. A comparison has been carried out between the results achieved through applying the two methods of analysis (morphometric and PCA). Afterwards, suitable measures are proposed for soil and water conservation. Topo sheets and ASTER DEM have been employed to demarcate the 43 sub-watersheds, to extract the drainage networks, and to compile the required thematic maps such as slope categories and elevation. LANDSAT 8 image (April-2015) is employed to generate land use/cover maps using ENVI (v 5.1) software. The soil map of the watershed has been digitized using Arc GIS software. Prioritization of the 43 sub-watersheds was performed using ten linear and shape parameters, and three parameters which are highly correlated with components 1 and 2. Subsequently, different sub-watersheds were prioritized by ascribing ranks based on the calculated compound parameters (Cp) using the two approaches. Comparison of the results revealed that prioritization of watersheds based on morphometric analysis is more consistent and serves for better decision making in conservation planning as compared with the PCA approach. The recommended soil conservation measures are prescribed in accordance with the specified priority, in order to avoid undesirable effects on land and environment. Sub-watersheds classified under high priority class are subjected to high erosion risk, thus, creating an urgent need for applying soil and water conservation measures. It is expected that decision makers will pay sufficient attention to the present results/information, activate programs encouraging soil conservation, integrated watershed management, and will continue working on the afforestation of the government-owned sloping lands. Such a viable approach can be applied at different parts of the rainfed highland areas to minimize soil erosion loss, and to increase infiltration and soil moisture in the soil profile, thus, reducing the impact of recurrent droughts and the possibility of flooding hazards.

Morphometric discrimination of wild from farmed Dybowski’s frog (Rana dybowskii) based on hindlimb length

Commercial farming of anuran species that are declining in the wild raises a need to discriminate wild from farmed frogs.We hypothesized wild frogs might have extended hindlimbs due to greater frequency or intensity of jumping relative to farmed frogs,highlighting a morphometric approach to discrimination of wild from farmed frogs using hindlimb length.In the present study,Dybowski＇s frog（Rana dybowskii） was used to test this hypothesis.We measured body mass（Mb） and hindlimb length（Lh） of 2-year old farmed frogs and wild frogs aged 2 to 5 years.Dybowski＇s frog demonstrated significant dimorphism in Mb and Lh.Mb was significantly greater among farmed 2-year old frogs in both sexes（p=0.000）,while only among females was Lh significantly greater for wild frogs（p=0.000）.Lh/Mb was used as an index for origin discrimination to eliminate the influence of Mb due to variation of husbandry conditions among farms.Mean Lh/Mb for farmed frogs was significantly lower than for wild frogs（p=0.000） in the 2-year old age class.Discrimination correctly classified 84.4% of farmed and 96.3% of wild male frogs.Among females,92.9% of farmed frogs and 90.1% wild frogs were correctly classified.The overall correctness of classification was 92.1% and 90.8% for males and females,respectively.However,Lh/Mb revealed variation with age,resulting in reduced discriminative power for frogs ≥3 years old.We introduced a coefficient Ce to adjust the Lh/Mb of frogs ≥3 years to the level equivalent to 2-year frogs.The adjustment achieved 89.5% for overall correctness of origin for wild males and 92.4% for wild females ≥3 years old.These results show that Lh/Mb is an effective index to discriminate wild from farmed Dybowski＇s frog.Since the physical demands of jumping are common among anurans,this index is also potentially applicable to other anuran species.

Cranial variation in allactagine jerboas(Allactaginae,Dipodidae, Rodentia): a geometric morphometric study

Allactaginae is a subfamily of dipodids consisting of four-and five-toed jerboas(Allactaga, Allactodipus,Orientallactaga, Pygeretmus, Scarturus) found in open habitats of Asia and North Africa. Recent molecular phylogenies have upended our understanding of this group's systematics across taxonomic scales. Here, I used cranial geometric morphometrics to examine variation across 219 specimens of 14 allactagine species(Allactaga major, A. severtzovi, Orientallactaga balikunica, O.bullata, O. sibirica, Pygeretmus platyurus, P. pumilio,P. shitkovi, Scarturus aralychensis, S. euphraticus,S. hotsoni, S. indicus, S. tetradactylus, S. williamsi)in light of their revised taxonomy. Results showed no significant sexual size or shape dimorphism. Species significantly differed in cranial size and shape both overall and as species pairs. Species identity had a strong effect on both cranial size and shape. Only a small part of cranial shape variation was allometric,with no evidence of unique species allometries, and most specimens fit closely to the common allometric regression vector. Allactaga was the largest,followed by Orientallactaga, Scarturus, and finally Pygeretmus. Principal component 1(PC1) separated O. bullata+O. balikunica+S. hotsoni(with inflated bullae along with reduced zygomatic arches and rostra) from A. major+A. severtzovi+O. sibirica(with converse patterns), while PC2 differentiated Orientallactaga(with enlarged cranial bases and rostra along with reduced zygomatic arches and foramina magna) from Scarturus+Pygeretmus(with the opposite patterns). Clustering based on the unweighted pair group method with arithmetic mean(UPGMA) contained the four genera, but S. hotsoni clustered with O. bullata+O. balikunica and O.sibirica clustered with A. major+A. severtzovi, likely due to convergence and allometry, respectively.

Three-dimensional Morphometric Analyses of Hominoid Lower Molars from Yuanmou in Yunnan Province,China

Analysis of 3D measurements of 13 landmarks on 72 Yuanmou Hominoid lower molars and some comparative samples, using Euclidean Distance Matrix Analysis (EDMA), show that the 3D morphometric features of Yuanmou Homi noid lower molars largely resemble those of Lufengpithecus. Compared with extant great apes and humans, both Yuanmou Hominoid and Lufengpithecus differ markedly from humans, whereas they show much resemblance to apes. The two Chinese hominoids and extant great apes are different from humans in many of the same ways. Moreover, 3D morphometric features of Yuanmou Hominoid lower molars differ from those of each of the three extant great apes in similar ways. Of the three, the orangutan is the closest match to Yuanmou Hominoid in tooth morphology. However, the exact implications of these similarities and differences remain to be determined.

Morphometric, Physicochemical, Thermal, and Rheological Properties of Rice (<i>Oryza sativa</i>L.) Cultivars <i>Indica ×Japonica</i>

The anther culture technical was applied to produce haploid lines of rice (Oryza sativa L.). The hybrids (K/ A92VM061, K/A92VM067, K/A92VM0611, K/A92VM719, K/A92VM720 and K/A92VM721) were obtained in order to generate new varieties from Indica and Japonica cultivars. Morphometric parameters of the grains were evaluated by image analysis. Flours were prepared from the whole rice grains and physicochemical, thermal and rheological properties, X-ray diffraction pattern and evaluation of color using the CIELAB system were assessed. The hybrids lines showed long (061, 611, 721), medium (719 and 720) and short (067) grains. The rice samples presented lipids (2.6% - 3.2%), protein (11% - 15%), total dietary fiber (8.4% - 10.2%), total starch (65% - 74%) and apparent amylose (5% - 32%) contents. Gelatinization temperature (Tp) was found in the range of 66.1℃ - 79.4℃ with enthalpy (ΔH) value between 3.4 - 8.1 J/g. The retrogradation parameters (temperature and ΔH) were lower than those for gelatinization in all samples. The rice samples presented A-type X-ray diffraction pattern. Rice pastes showed a non-Newtonian behavior and the brightness (L*) characterize the color of the samples. Hybrid rice grains presented morphometric properties more similar to Japonica than Indica variety. Rice hybrid had higher protein content than Indica variety. Apparent amylose, viscosity and gelatinization temperature varied significantly among hybrids and varieties.

Lithostructural controls in Douala-Buea Region landscape (SW Cameroon margin):Insights from morphometric analysis

The Douala-Buea Region(DBR;4°-5°N,9°-10°30’E),a portion of the southern Cameroon passive margin,developed on the Proterozoic granito-gneissic basement and the Cretaceous to Recent volcanosedimentary cover,was explored in order to evaluate the lithology and tectonics controls on its landscape usingthequalitativeandquantitative geomorphological analyses based on 30 m resolution Digital Elevation Models(DEM)and field evidences.This area displays an uneven and complex landscape differentiated into four morphological units:Douala,Nkondjock,Kumba and Buea Units.The Douala Unit corresponds to the Coastal Plain and is extended on the Douala-Kribi/Camposedimentarybasin.The Nkondjock Unit represents a middle plateau developed on a Proterozoic granito-gneissic basement.The Kumba Unit matches with the southernmost volcanic Western Cameroon High Plateau.The Buea Unit corresponds to the Mount Cameroon stratovolcano.The relief analysis through mountain-front sinuosity(Smf,1.19–1.43)and relative relief ratio(Rr,0.10–2.31)vary from one unit to another.The drainage network analysis including drainage patterns,stepped longitudinalriverprofiles,theassociated morphometric indexes,notably the concavity index(IC,0.56–0.88),deviation(D,267–2912 m),basin asymmetry factor(A_(F),6.37–42.12),hypsometric integral(HI,0.10–0.29),valley floor width to valley height ratio(V_(f),0.36–2.75),elongation ratio(R_(e),0.30–0.50),watershed slope(S,6.88–88.88)and stream length gradient index(SL,0–3332)vary from one basin to another too.These results also show the heterogeneous and uneven DBR landscape controlled by its lithology and active tectonics.The drainage system reveals concave to concavo-convex longitudinal river profile shapes with knick points,asymmetric basins,and fault reactivations,and highlights the tectonics activity that used to occur through the time and their control on the morphology.Together,they document the lithostructural controls on the DBR landscape evolution,extendable in the whole SW Cameroon margin.

Quantitative Analysis of Flabellophyton from the Ediacaran Lantian Biota, South China: Application of Geometric Morphometrics in Precambrian Fossil Research

As a more efficient quantitative method for morphological analysis, geometric morphometrics is applied to the flabellate fossils Flabellophyton including Flabellophyton lantianensis and Flabellophyton strigata from Neoproterozoic Ediacaran Lantian biota, South China. Both traditional morphometric analysis （including diverging angle, length, and width distribution） and geometric morphometric analysis [including superposition of normalized area analysis, non-metric multidimensional scaling （n-MDS）, Bray-Curtis similarity cluster analysis （CLUSTER） and analysis of similarities （ANOSIM）[ were performed. The results indicate there are great interspecific morphological differences between F. lantianensis and F. strigata, in addition to some intraspecific variations within each species. Here we preliminarily suggest that the previously defined Flabellophyton should be subdivided into 2 genera at least： one marked by the dark transverse striated structure, and the other without. This recharacterization and reclassification work still needs further careful observation and comprehensive analysis with large number of flabeilate fossils combining morphology, structure, population, and community-ecology study.

Species limits in the Elegant Pitta (Pitta elegans) complex from Wallacea based on bioacoustic and morphometric analysis

Background:The Elegant Pitta(Pitta elegans)complex displays a remarkable diversity of morphological and bioacoustic traits across five taxa currently recognized as subspecies.They differ in plumage characteristics(such as red versus black belly patches;supercilium color and extent;and white versus black throats),in lifestyle(resident versus migratory)and in vocalizations.We investigated the morphological,bioacoustic and ecological differences across all taxa after recent studies demostrated the importance of these traits in recognizing biological species limits across pittas.Methods:Morphometric analysis was carried out by measuring tarsus,wing,tail and bill lengths of 15 specimens at the Natural History Museum,UK,and plumages were inspected across 106 unique individuals from four different repositories.Bioacoustic analysis was based on 134 range-wide sound recordings.Two types of calls,territorial calls and alarm calls,were analyzed using different sets of parameters.Principal component analysis and the Isler Criterion were applied to the measurements.Playback trials were conducted to explore the levels of response of each taxon to the call types of the other taxa.Results:The territorial call of concinna exhibits a distinct two-element motif,while elegans,maria and virginalis utter a three-element motif in which the first two elements are given in quick succession.On the other hand,vigorsii,produces both two-element and three-element motifs with longer breaks in between elements.As further corroborated by the playback trials,the three taxa elegans,virginalis and maria form a tight vocal cluster,whereas each concinna and vigorsii are distinct.The alarm call turned out to be less diagnostic even though most taxa did roughly separate into different vocal clusters.Morphometric analysis failed to produce strong differences,but plumage distinctions among multiple taxa are pronounced.Conclusions:We suggest splitting the Elegant Pitta into three biological species based on bioacoustic and—less so—plumage evidence:(1)Temminck’s Elegant Pitta P.elegans(including subspecies elegans,virginalis and maria),(2)Wallace’s Elegant Pitta P.concinna(monotypic),and(3)Banda Elegant Pitta P.vigorsii(monotypic).

Prioritization of W. Mujib Catchment (South Jordan) through Morphometric and Discriminant Analysis, GIS, and RS Techniques

GIS and remote sensing were utilized for prioritizing the W. Mujib catchment. Fifty three fourth-order sub-watersheds were prioritized based on morphometric analysis of linear and shape parameters. ASTER DEM (v.2), topographical maps, and Arc GIS (10.1) software, have been employed to delineate the 53 sub-basins, to extract the drainage networks, and to compute the required basic, linear, and shape parameters, and to compile the necessary thematic maps such as elevation and slope categories. The land use/land cover map was generated using ERDAS Imagine (2015), LANDSAT 8 image, and supervised classification (Maximum Likelihood Method). Soil map was digitized using the Arc GIS tool. Each sub-basin is prioritized by assigning ranks based on the calculated compound parameter (Cp). The final score for each sub-basin is ascribed as per erosion threat. The 53 sub-watersheds were grouped into four categories of priority: very high (15 sub-basins, 28.3% of the total), high (17 sub-basins, 32% of the total), moderate (16 sub-basins, 30.2% of the total), and low (5 sub-basins, 9.5% of the total). Sub-basins categorized as very high and high priority (60.3% of the total) are subjected to high erosion risk, thus, creating an urgent need for applying soil and water conservation measures. The validity of the prioritized four groups was tested statistically by means of Discriminant Analysis (DA), and a significant difference was found between the four priority classes. A relatively complete separation exists between the recognized priority classes;thus, they are statistically valid, distinct, and different from each other. The present results intend to help decision makers pay sufficient attention to soil and water conservation programs, and to encourage tree plantation over the government-owned sloping land. Such procedures are essential in order to minimize soil erosion loss, and to increase soil moisture on farms, thus, reducing the impact of recurrent droughts and the possibility of flooding downstream.

Prioritization of Sub-Watersheds in a Large Semi-Arid Drainage Basin (Southern Jordan) Using Morphometric Analysis, GIS, and Multivariate Statistics

GIS-based morphometric analysis was employed to prioritize the W. Mujib-Wala watershed southern Jordan. Seventy six fourth-order sub-watersheds were prioritized using morphometric analysis of ten linear and shape parameters. Each sub-watershed is prioritized by designated ranks based on the calculated compound parameter (Cp). The total score for each sub-basin is assigned as per erosion threat. The 76 sub-basins were grouped into four categories of priority: very high (12 sub-basins, 15.8% of the total), high (32 sub-watersheds, 42.1% of the total), moderate (25 sub-watersheds, 32.9% of the total), and low (7 sub-watersheds, 9.2% of the total). Sub-watersheds categorized as very high and high are subjected to high erosion risk, thus creating an urgent need for applying soil and water conservation measures. The relative diversity in land use practices and land cover, including variation in slope and soil types, are considered in proposing suitable conservation structures for sub-watersheds connected to each priority class. The adaptation of soil conservation measures priority-wise will reduce the erosivity effect on soil loss;while increasing infiltration rates;and water availability in soil profile. Principal component analysis (PCA) reduces the basic parameters and erosion risk parameters to three components, explaining 88% of the variance. The relationships of these components to the basic and erosion risk parameters were evaluated, and then the degree of inter-correlation among the morphometric parameters was explored. The verification of priority classes obtained through morphometric analysis was tested using Discriminant Analysis (DA). The results show a complete separation existing between the identified priority classes. Thus, soil erosion risk and geomorphic conditions are found entirely different from one class to another. The present results are intended to help decision makers to plan for efficient soil and water conservation measures to achieve future agricultural sustainability in the rainfed highlands of Jordan.

The Biodiversity of Shrimp Genus Artemia from Russian Lakes:Morphometric,Cytogenetics and DNA-analysis

Shrimps of genus Artemia are the inhabitants of continental and marine waters with salinity of 70 to 350 g/l and above.Artemia is able to survive in the conditions in which other animals cannot exist.This is due to adaptations:effective osmoregulation system,the ability to synthesize of respiratory pigment(hemoglobin)and diapauses cysts(Litvinenko at.al.,2009).Cysts of this

Morphometric Studies of Human Coronary Artery Trees in Healthy and Disease

Objective According to the report from American Heart Association(AHA),cardiovascular diseases(CVDs)are the leading causes of death globally,and coronary artery disease(CAD),known as coronary atherosclerotic plaques,accounts for over 30%of cardiovascular diseases.Therefore,it is of great clinical significance to study the relationship between coronary bifurcations morphometrical feature change and coronary artery disease.Although coronary atherosclerosis has been extensively investigated,there is a lack of in-deep study on the differences in morphometric features between optimal and realistic geometry of coronary arterial trees.The purpose of the present paper is to determine the morphological changes in patients with CAD lesion compared with non-coronary artery disease(non-CAD)subjects.Methods Due to the difficulty of studying the coronary bifurcations in vivo,image-based in vitro anatomical 3D models have been widely used as a noninvasive method for morphometric measurement and clinical diagnosis of the coronary bifurcations.With the development of coronary computed tomography angiography(CTA)hardware and software technologies,the CTA imaging technique has been shown a promising application in the characterization,visualization,and identification of coronary artery disease in recent decades.The CTA images used to reconstruct three-dimensional(3D)coronary arterial trees are from Asia populations(Southern Chinese populations),including five cadavers without CAD lesion and 102 patients with CAD lesion.The best fit artery diameter was calculated as twice the average radius between the points in the centerlines and the points on the coronary arterial inner wall.The bifurcation angles between larger daughter artery and smaller daughter artery were determined by the intersection angle of their centerlines.Murray’s law was introduced to assess the deviation of the realistic vascular networks from its optimal state.Results Based on the morphometric analysis of coronary artery bifurcations in non-CAD subjects and patients with CAD lesion subjects,the most important finding is that morphological feature parameters of non-CAD subjects are closer to the optimal values than those of patients with CAD lesion.Moreover,by comparing the morphometric data between the left and right coronary arteries,the right coronary artery exhibits a structure closer to the optimal one in morphological feature than the left coronary artery.In addition,coronary arterial trees with CAD lesion have higher asymmetry and larger area expansion ratio(AER)than those of the coronary arterial trees without CAD lesion.Conclusions We morphologically found that the coronary arterial trees with CAD lesion and left are more likely to deviate from the optimal structure predicted by Murray’s law than those without CAD lesion and right.The degree to which coronary arterial system deviating from their optimal state may directly affect the incidence of coronary artery disease.This computer morpho-logical analysis strategy is illustrated to be effective in the distinguishing of the geometric differences between the healthy and diseased coronary arteries,and the analysis method may have a large potential in cardiovascular disease earlier diagnosis.

Intraspecific morphometric diversity of barramundi (Lates calcariferBloch, 1790) in the waters of southern Bangladesh

A total of 160 barramundi's (Lates calcarifer Bloch, 1790) sampled from four rivers (Tentulia, Balaswar, Bakkhali, and Andarmanik) along the southern coastal region of Bangladesh were investigated in terms of morphometric characters to reveal the intraspecific variation. Twenty-five morphometric measurements were extracted using the conventional method and subjected to multivariate analyses (i.e., principal component analysis (PCA), discriminate function analysis (DFA), cluster analysis (CA)) to distinguish individuals from different rivers. The result demonstrated that twenty-two out of 25 measurements was statistically significant (Univariate ANOVA) among all four populations. PCA analysis of morphometric characters resulted in two principal components, PC I and PCⅡ which accounted for 79.25% and 4.28% of the total data variance. PC I-PC Ⅱ plot explained 83.53% of total variance differentiated the population of L. calcarifer into two groups. Discriminate analysis correctly classified about 88.1% of the examined fish into the four areas. The UPGMA dendrogram showed that Bakkhali populations were the most morphologically different populations in comparison to other populations, while Andarmanik and Balaswar populations were very close to each other. The strong morphometric variation between Bakkhali and Tentulia, Andarmanik and Balaswar was observed in the present study, suggested the evidence of the separate stock population of barramundi in these locations, which might require distinct stock management strategies for resource sustainability in the waters of southern Bangladesh. However, if these findings are supported by further molecular markers and geometric morphometry, this would be a strong indication of different stocks of this population in the four rivers of southern Bangladesh.

Morphometric relationships and reproductive maturation of the mudskipper, Periophthalmus barbarus from subsistence catches in the mangrove swamps of IMO estuary, Nigeria

From April 1992 and March 1993, morphometric characteristics and reproductive maturation were studied in Periophthalmus barbarus from subsistence catches in the mangrove swamps of Imo estuary. Morphometric equations for males and females at different stages of ovarian maturation are presented. Generally males were significantly heavier in weight than females, but the latter were heavier than the former of the same body length. Females with developing and mature ovaries were generally heavier than immature females of the same length. Analyses of the gonadosomatic index and percentage of mature males and females indicate all year\|round breeding such that: February\|May was spawning, June\|October postspawning and November to January recovery period. Fecundity varied between 900 and 23933 eggs per spawn and increased with fish size.

Variation in seed morphometric characters, oil content and azadirachtin content of seeds, in vitro shoot cultures and callus cultures among different populations of Azadirachta indica

We studied seed morphometric characters, oil content and azadirachtin content in a medicinally important and insect repellent tree species Azadirachta indica A.Juss. Ten populations were selected from two central Indian states of Madhya Pradesh and Chhattisgarh and twenty candidate plus trees were selected from each population based on tree attributes. Azadirachtin content was estimated in seeds, in vitro shoots and callus cultures through HPLC. Significant variation（p ／ 0.05） was observed in seed morphometric characters, oil content and azadirachtin content among the ten populations. Maximum value for 100 seed weight was obtained in seeds of Raigarh（30.51 g） which was on par with seeds of Bargi（28.37 g）and Chhatarpur（26.36 g）. The ratio of highest kernel to seed was also obtained in seeds of Raigarh（60.11%）.Kernels from Chhatarpur population recorded the highest oil content（24.20%）, which was equal to that in Sihore（23.75%） and Katni（21.75%）. We recorded highly significant variation in azadirachtin content in seed samples,in vitro shoot samples and callus cultures. Maximum azadirachtin was present in the seeds of Gwalior（0.882%）which was on par with azadirachtin content in seeds of Chhatarpur, Katni, Sihore, Shahdol and Chhindwara.Maximum azadirachtin in the in vitro shoots was present in the cultures of Gwalior（0.218%） which was statistically on par with Sihore（0.176%）. Similarly, in callus cultures of nodal segments maximum azadirachtin was recorded in the Gwalior population（0.033%） which was statistically on par with Sihore（0.028%）. The trees of Gwalior and Sihore populations can be produced in large numbers through vegetative propagation to obtain seeds with higher azadirachtin content.