Aim: To assess the protective efficacy of vitamin E to counteract the reactive oxygen species (ROS) mediated damage onsperm motility, viability and lipid peroxidation. Methods: Human semen samples were obtained from t...Aim: To assess the protective efficacy of vitamin E to counteract the reactive oxygen species (ROS) mediated damage onsperm motility, viability and lipid peroxidation. Methods: Human semen samples were obtained from the local hospi-tal. The split seminal fractions freed of seminal plasma were reconstituted in Ringer-Tyrode and subjected to varied vita-min E concentrations (0.1 - 2 mmol/L). Results: Dose-dependent improvement in both motility and viability accom-panied by concomitant decrease in malondialdehyde (MDA--an end product of lipid peroxidation) following vitamin Esupplementation was noticed. Conclusion: Vitamin E protects against the ROS mediated damage on spermatozoa.Vitamin E supplementation could be of clinical importance for prolonged spermatozoal storage whenever needed. (AsianJ Androl 1999 Sep; 1: 151 - 154 )展开更多
This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoosp...This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient＇s first consultation, semen parameters were measured using SMASTM or CellSoft 3000TM, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups： reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases （33.3%）. Sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.01; P 〈 0.05）, and progressive motility （P 〈 0.01; P 〈 0.01） were markedly lower in the reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.05; P 〈 0.01）, mALH （P 〈 0.05; P 〈 0.01）, and progressive motility （P 〈 0.05; P 〈 0.01） also showed inverse correlations with the logarithmic transformed reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.展开更多
Polychlorinated biphenyls （PCBs） are common environmental contaminants that represent a considerable risk to reproductive toxicity in exposed human populations. Although some experimental studies have suggested an a...Polychlorinated biphenyls （PCBs） are common environmental contaminants that represent a considerable risk to reproductive toxicity in exposed human populations. Although some experimental studies have suggested an association between the levels of PCBs and semen quality, the direct effects of PCBs on human sperm parameters remain largely unexplored. To this aim, a short-term in vitro incubation experiment that better imitated the putative exposure of sperm to Aroclor 1254 （a commercial PCB mixture） in male reproduction tissue was conducted. Human sperm were incubated with various concentrations （0, 1, 5, or 25 mg ｜^-1） of Aroclor 1254 for different amounts of time （3 and 6 h） in vitro. Sperm motility parameters were analyzed with computer-assisted sperm analysis （CASA）. The proportion of sperm with high mitochondrial membrane potential （ΔΨm） and the levels of intracellular reactive oxygen species （ROS） were detected to explore the probable cause of sperm impairment. Human sperm exposed to continuous Aroclor 1254 exhibited： （i） reduced sperm motility and kinematic parameters, （ii） a proportion of sperm with high ΔΨm that decreased in a dose-dependent manner （P 〈 0.05）, and （iii） increased levels of ROS compared with controls （P 〈 0.05）. In conclusion, Aroclor 1254 can decrease sperm motility, which may culminate in increased ROS and general mitochondrial dysfunction, thus affecting the fertilization potential of sperm. Our findings suggest a broader understanding of the effect of Aroclor 1254 on human soerm.展开更多
文摘Aim: To assess the protective efficacy of vitamin E to counteract the reactive oxygen species (ROS) mediated damage onsperm motility, viability and lipid peroxidation. Methods: Human semen samples were obtained from the local hospi-tal. The split seminal fractions freed of seminal plasma were reconstituted in Ringer-Tyrode and subjected to varied vita-min E concentrations (0.1 - 2 mmol/L). Results: Dose-dependent improvement in both motility and viability accom-panied by concomitant decrease in malondialdehyde (MDA--an end product of lipid peroxidation) following vitamin Esupplementation was noticed. Conclusion: Vitamin E protects against the ROS mediated damage on spermatozoa.Vitamin E supplementation could be of clinical importance for prolonged spermatozoal storage whenever needed. (AsianJ Androl 1999 Sep; 1: 151 - 154 )
文摘This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient＇s first consultation, semen parameters were measured using SMASTM or CellSoft 3000TM, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups： reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases （33.3%）. Sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.01; P 〈 0.05）, and progressive motility （P 〈 0.01; P 〈 0.01） were markedly lower in the reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.05; P 〈 0.01）, mALH （P 〈 0.05; P 〈 0.01）, and progressive motility （P 〈 0.05; P 〈 0.01） also showed inverse correlations with the logarithmic transformed reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.
基金This work was supported by the National Natural Science Foundation of China （Grant Numbers 81571511, 81370711, and 30901603）, the Science and Technology Foundation of Shandong （Grant Number 2010GSF 10814）, the Shandong Provincial Natural Science Foundation （Grant Number ZR2013HM090）, and the Science Foundation of Qilu Hospital of Shandong University, Fundamental Research Funds of Shandong University （Grant Numbers 2015QLMS24, 2016QLQN24 and 2015QLQN50）.
文摘Polychlorinated biphenyls （PCBs） are common environmental contaminants that represent a considerable risk to reproductive toxicity in exposed human populations. Although some experimental studies have suggested an association between the levels of PCBs and semen quality, the direct effects of PCBs on human sperm parameters remain largely unexplored. To this aim, a short-term in vitro incubation experiment that better imitated the putative exposure of sperm to Aroclor 1254 （a commercial PCB mixture） in male reproduction tissue was conducted. Human sperm were incubated with various concentrations （0, 1, 5, or 25 mg ｜^-1） of Aroclor 1254 for different amounts of time （3 and 6 h） in vitro. Sperm motility parameters were analyzed with computer-assisted sperm analysis （CASA）. The proportion of sperm with high mitochondrial membrane potential （ΔΨm） and the levels of intracellular reactive oxygen species （ROS） were detected to explore the probable cause of sperm impairment. Human sperm exposed to continuous Aroclor 1254 exhibited： （i） reduced sperm motility and kinematic parameters, （ii） a proportion of sperm with high ΔΨm that decreased in a dose-dependent manner （P 〈 0.05）, and （iii） increased levels of ROS compared with controls （P 〈 0.05）. In conclusion, Aroclor 1254 can decrease sperm motility, which may culminate in increased ROS and general mitochondrial dysfunction, thus affecting the fertilization potential of sperm. Our findings suggest a broader understanding of the effect of Aroclor 1254 on human soerm.
