COMPARATIVE STUDY OF PHOSPHOTYROSYL PROTEIN PHOSPHATASE OF MICE NORMAL LIVER, REGENERATING LIVER, AND MICE H22A HEPATOMA ASCITES CELL

In regenerating liver of mice, marked increase of the activity of phosphotyrosyl protein phosphatase (PTPP) in cytosol was observed. The PTPP activity varied with time and reached the highest level between 24 to 48 hours after partial hepatectomy. In H22a cells the PTPP activity found in every subcellular fraction was lower than that of the normal liver. The PTPP activity was mostly concentrated in lysosomes of normal liver, but mainly distributed in nucleus, cytosol and microsome of regenerating liver. In H22a cells PTPP activity seemed distribute evenly. Five similar major PTPP peaks (I-V) were obtained on DEAE cellulose chromatography of cytosols from all three of liver cells studied. However, two additional PTPP peaks, a and b, were also obtained from cytosol of liver.

甲胎蛋白对肝癌细胞生长的影响 Ⅰ.人AFP对小鼠H_(22)腹水型肝癌细胞RNA合成的促进作用

用亲和层析法从3-5月龄引产胎儿肌肉组织中提纯AFP。体外用~3H-UR参入的方法,从转录水平观察AFP对小鼠H_(22)腹水型肝癌细胞生长的影响。结果表明,人AFP能显著地直接促进该肿瘤细胞RNA的合成;人血白蛋白和去除AFP后胎儿肌肉匀浆液等对照均无显著作用;AFP抗血清能显著地清除AFP的这种促进作用。用小鼠S_(180)乳腺肉瘤细胞作为对照表明,AFP促肿瘤生长无严格的组织特异性。

地塞米松对小鼠H_(22)肿瘤生长及血管内皮生长因子表达的影响

目的 探讨地塞米松对小鼠H2 2 肿瘤生长及血管内皮生长因子 (VEGF)表达的影响。方法 BALB c小鼠皮下接种H2 2 肿瘤细胞 ,腹腔注射地塞米松 ,每天测肿瘤直径。用抗CD31单抗对肿瘤组织做免疫组化微血管计数。噻唑蓝法检测地塞米松对体外培养的H2 2 细胞和人脐静脉内皮细胞ECV 30 4增殖的影响。RT PCR法测定肿瘤组织和体外培养细胞的VEGFmRNA表达。结果 地塞米松可以显著抑制体内H2 2 肿瘤的生长 ,给药组肿瘤体积与对照组比较P <0 .0 5。给药组微血管计数和VEGFmRNA表达比对照组显著减少。体外实验中 ,随着地塞米松浓度的增加 ,ECV 30 4细胞的增殖率降低 ;H2 2 细胞中VEGFmR NA表达下降。结论 地塞米松可以显著抑制小鼠H2 2 肿瘤的生长。地塞米松对体内H2 2 肿瘤组织和体外H2 2 细胞的VEGFmRNA表达均有显著的抑制作用。地塞米松对肿瘤细胞VEGF表达的抑制作用可能是其抗H2 2 肿瘤及抗血管生成的一个重要原因。

EFFECTS OF ACUMOXI ON IL2-IFN-NKC IMMUNOREGULATORY NETWORK AND ITS RELATED MACROPHAGE-IL1-Th NETWORK AND ON TUMOR——Observation on the Effect of Acumoxi on the Macrophage-IL1-Th Network and on the Metastasis of Hepatoma

Objective: To observe the effect of acumoxi (acupuncture and moxibustion) on macrophage (Mφ)-lL1-Th net-work and hydroperitoneum hepatoma (H 22) metastasis in mice. Methods: A total of 36 BALB/ c male mice bearing H 22 are randomly divided into control, acupuncture and acumoxi groups with 12 cases in each group. In the later 2 groups, Dazhui (GV 14) and Guanyuan (CV 4) are punctured once daily, continuously for 18 days, and in acumoxi group, the two acupoints were also moxibustioned alternatively with moxa stick once every day. After killing the mice, the tissue samples of the 3 groups are treated routinely step by step and analyzed by means of colorimetric analysis for determining the phagocytic function of the macrophages; and the content of IL1 of the Mφ supernatant is assayed with serum plate agglutination (SPA)-Ig floral hoop method of T helper cell (Th) monoclonal antibody; the weight of the reniportal lymph node, the kidney and the lung, and the number of the cancerous nodes on the pulmonary surface are calculated. Results: After acupuncture and moxibustion treatment, the immunoregulatory network indices of acumoxi group increase obviously compared with those of control group(P<0.01), showing an anti-metastasis effect of acumoxi on H 22. Conclusion: Results of the present study and those of our former research prove that acupuncture and moxibustion can suppress the tumor growth and H 22 metastasis by the enhancement of the immunoregulatory network.

榄香烯复合瘤苗主动免疫抗瘤效应增强途径的比较研究

将ＢＡＬＢ／ｃ 小鼠的Ｈ２２ 腹水型肝癌和６１５ 系小鼠的Ｌ６１５ 白血病细胞经榄香烯或／和热休克等不同处理制成瘤苗， 进行体内主动免疫实验和致敏脾细胞的体外细胞毒活性实验。结果表明： 热休克和榄香烯复合瘤苗比单一因素处理的瘤苗免疫效果好， 经Ｈ２２和Ｌ６１５ 复合瘤苗免疫、攻击后两个月动物存活率分别达６０－０％ 、８５－０％ （ Ｐ＜０－０１）， 并且能够明显提高死亡小鼠的平均存活天数。瘤苗免疫动物脾细胞的细胞毒活性分别达５８－８％ 、６９－７％ （ Ｐ ＜ ０－０１） 。在制备复合瘤苗时先加入榄香烯后再热休克的免疫效果较好， 这在Ｌ６１５ 瘤苗更为明显， 从而为今后瘤苗免疫在临床上的应用提供了进一步的实验依据。

叶下珠复方Ⅱ号对小鼠H22肝癌移植瘤生长的抑制作用

目的观察叶下珠复方Ⅱ号对小鼠H22肝癌移植瘤生长的抑制作用,并探讨其可能的作用机制。方法取昆明小鼠40只,建立小鼠H22肝癌移植瘤模型,分为模型组、叶下珠复方Ⅱ号高剂量组(37.5g/kg)、低剂量组(18.75g/kg)和环磷酰胺(20mg/kg)治疗组。叶下珠复方Ⅱ号高、低剂量组均灌胃给药,环磷酰胺组采用腹腔注射,每日1次,连续8d。末次给药24h后,测定瘤体质量,计算抑瘤率;检测血清IL-2和TNF-а含量。结果叶下珠复方Ⅱ号高、低剂量组瘤重明显低于模型组,差异均有显著性意义(P<0.01),但与环磷酰胺组比较,差异均无显著性意义(P>0.05)。叶下珠复方Ⅱ号高、低剂量组的抑瘤率分别为67%和58%,与环磷酰胺组接近。叶下珠复方Ⅱ号高剂量组血清IL-2水平明显高于模型组(P<0.05)。叶下珠复方Ⅱ号高、低剂量组血清TNF-а水平明显低于模型组,差异有显著性意义(P<0.05)。结论叶下珠复方Ⅱ号有较好的抑制小鼠H22肝癌移植瘤生长作用,作用机制与调节免疫,升高IL-2水平和抑制TNF-а水平有关。

凯息平口服液对H_(22)荷瘤小鼠的抑瘤生长作用及抑瘤机理探讨

凯息平口服液对小鼠移植性H2 2 实体瘤有明显的抑瘤生长作用 ,2 7.0、18.0、9.0g/kg剂量的抑瘤率分别 4 7.6 %、4 7.8%和 33.8% ;对H2 2 腹水癌小鼠有一定的延长生存期的作用 ,强于阳性对照药复方天仙胶囊 ,且没有氟尿嘧啶 (5 FU)所表现的毒副作用。对体外培养的H2 2 细胞有一定程度的抑制作用 ,最低有效药物浓度可达 2 5mg/L ,抑制作用可维持 4 8h以上。但对培养的脾T细胞 ,其剂量达 80 0mg/L时才表现出抑制增殖的作用。表明凯息平口服液对肿瘤细胞有独特的抑制作用 ,绝非细胞毒所致。实验还表明 :凯息平口服液能增强荷瘤鼠的T、B淋巴细胞增殖能力 ;对自然杀伤细胞 (NK)的活性也有明显的促进作用 ,同时明显增强腹腔巨噬细胞 (MΦ)的吞噬活性 ,显示了其在抗肿瘤免疫中具有重要的作用。

甲胎蛋自对H－22腹水肝癌小鼠脾淋巴细胞增殖的影响

用抗甲胎蛋白（ａｎｔｉ－ＡＦＰ）的单克隆抗体亲和层析柱从脐带血中提纯ＡＦＰ。通过ＭＴＴ法体外观察人ＡＦＰ对Ｈ－２２腹水肝癌小鼠脾淋巴细胞增殖的影响。结果表明，Ｈ－２２小鼠脾淋巴细胞经不同浓度ＡＦＰ（１２．５－１００μｇ／ｍｌ）处理后，对刀豆素Ａ（ＣｏｎＡ）诱导的增殖反应明显减弱，抑制率达３９．８％－６６％，并有一定的浓度依赖关系；而用不同浓度的人血清白蛋白（ＨＳＡ，３．１～１００μｇ／ｍｌ）体外处理，则无明显抑制作用。另外，我们用反证法证实，ＡＦＰ抗体（５５、１１０μｇ／ｍｌ）能明显阻断人ＡＦＰ（５０、１００μｇ／ｍｌ）对Ｈ－２２小鼠脾淋巴细胞增殖的抑制作用。