期刊文献+
共找到14,723篇文章
< 1 2 250 >
每页显示 20 50 100
Discovery and inspiration of large-and medium-sized glutenite-rich oil and gas fields in the eastern South China Sea:An example from Paleogene Enping Formation in Huizhou 26 subsag,Pearl River Mouth Basin 被引量:1
1
作者 XU Changgui GAO Yangdong +4 位作者 LIU Jun PENG Guangrong LIU Pei XIONG Wanlin SONG Penglin 《Petroleum Exploration and Development》 SCIE 2024年第1期15-30,共16页
Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and ... Based on the practice of oil and gas exploration in the Huizhou Sag of the Pearl River Mouth Basin,the geochemical indexes of source rocks were measured,the reservoir development morphology was restored,the rocks and minerals were characterized microscopically,the measured trap sealing indexes were compared,the biomarker compounds of crude oil were extracted,the genesis of condensate gas was identified,and the reservoir-forming conditions were examined.On this basis,the Paleogene Enping Formation in the Huizhou 26 subsag was systematically analyzed for the potential of oil and gas resources,the development characteristics of large-scale high-quality conglomerate reservoirs,the trapping effectiveness of faults,the hydrocarbon migration and accumulation model,and the formation conditions and exploration targets of large-and medium-sized glutenite-rich oil and gas fields.The research results were obtained in four aspects.First,the Paleogene Wenchang Formation in the Huizhou 26 subsag develops extensive and thick high-quality source rocks of semi-deep to deep lacustrine subfacies,which have typical hydrocarbon expulsion characteristics of"great oil generation in the early stage and huge gas expulsion in the late stage",providing a sufficient material basis for hydrocarbon accumulation in the Enping Formation.Second,under the joint control of the steep slope zone and transition zone of the fault within the sag,the large-scale near-source glutenite reservoirs are highly heterogeneous,with the development scale dominated hierarchically by three factors(favorable facies zone,particle component,and microfracture).The(subaqueous)distributary channels near the fault system,with equal grains,a low mud content(<5%),and a high content of feldspar composition,are conducive to the development of sweet spot reservoirs.Third,the strike-slip pressurization trap covered by stable lake flooding mudstone is a necessary condition for oil and gas preservation,and the NE and nearly EW faults obliquely to the principal stress have the best control on traps.Fourth,the spatiotemporal configuration of high-quality source rocks,fault transport/sealing,and glutenite reservoirs controls the degree of hydrocarbon enrichment.From top to bottom,three hydrocarbon accumulation units,i.e.low-fill zone,transition zone,and high-fill zone,are recognized.The main area of the channel in the nearly pressurized source-connecting fault zone is favorable for large-scale hydrocarbon enrichment.The research results suggest a new direction for the exploration of large-scale glutenite-rich reservoirs in the Enping Formation of the Pearl River Mouth Basin,and present a major breakthrough in oil and gas exploration. 展开更多
关键词 Pearl River mouth Basin Huizhou Sag Huizhou 26 subsag PALEOGENE Enping Formation GLUTENITE large-and medium-sized oil and gas field
下载PDF
Effect of Mouth Opening Training Stick Combined with Oral Massage on Mouth Opening Difficulty in Head and Neck Cancer Patients after Treatment
2
作者 Jinhua Liu Qiong Wen 《Journal of Biosciences and Medicines》 2024年第6期43-51,共9页
Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient samp... Objective: To study the application effect of mouth-opening training sticks combined with oral massage on patients with mouth-opening difficulty after treatment for head and neck cancer. Methods: Using convenient sampling, 60 patients with mouth-opening difficulty after treatment for head and neck cancer admitted to the Oncology Department from February 2022 to October 2023 were selected for a 2-week exercise and nursing program. The patients were divided into a control group (February 1, 2022 to November 30, 2022) and an observation group (December 1, 2022 to October 31, 2023), with 30 patients in each group. The control group underwent routine mouth-opening functional exercises combined with cork for oral support training, while the observation group underwent routine mouth-opening functional exercises combined with oral massage and mouth-opening training sticks for oral support training. The mouth-opening degree, mouth-opening difficulty level, comfort level, compliance, and quality of life were observed in both groups. Results: Before the intervention, there were no statistically significant differences in mouth-opening degree and mouth-opening difficulty level between the two groups (P > 0.05). After the intervention, the mouth-opening degree, mouth-opening difficulty level, oral comfort level, compliance, and QLICP-HN scores in the observation group were all better than those in the control group, with statistically significant differences (P < 0.05). Conclusion: The combination of mouth-opening training sticks and oral massage with mouth-opening functional exercises can effectively improve the degree of mouth-opening difficulty, enhance oral comfort, increase compliance with mouth-opening exercises, and improve quality of life. 展开更多
关键词 Head and Neck Cancer Difficulty in Opening the mouth Open mouth Training Stick Oral Massage Rehabilitation Effect
下载PDF
Coupling relationship and genetic mechanisms of shelf-edge delta and deep-water fan source-to-sink:A case study in Paleogene Zhuhai Formation in south subsag of Baiyun Sag,Pearl River Mouth Basin,China
3
作者 TANG Wu XIE Xiaojun +5 位作者 XIONG Lianqiao GUO Shuai XU Min XU Enze BAI Haiqiang LIU Ziyu 《Petroleum Exploration and Development》 SCIE 2024年第3期589-603,共15页
The coupling relationship between shelf-edge deltas and deep-water fan sand bodies is a hot and cutting-edge field of international sedimentology and deep-water oil and gas exploration.Based on the newly acquired high... The coupling relationship between shelf-edge deltas and deep-water fan sand bodies is a hot and cutting-edge field of international sedimentology and deep-water oil and gas exploration.Based on the newly acquired high-resolution 3D seismic,logging and core data of Pearl River Mouth Basin(PRMB),this paper dissected the shelf-edge delta to deep-water fan(SEDDF)depositional system in the Oligocene Zhuhai Formation of Paleogene in south subsag of Baiyun Sag,and revealed the complex coupling relationship from the continental shelf edge to deep-water fan sedimentation and its genetic mechanisms.The results show that during the deposition of the fourth to first members of the Zhuhai Formation,the scale of the SEDDF depositional system in the study area showed a pattern of first increasing and then decreasing,with deep-water fan developed in the third to first members and the largest plane distribution scale developed in the late stage of the second member.Based on the development of SEDDF depositional system along the source direction,three types of coupling relationships are divided,namely,deltas that are linked downdip to fans,deltas that lack downdip fans and fans that lack updip coeval deltas,with different depositional characteristics and genetic mechanisms.(1)Deltas that are linked downdip to fans:with the development of shelf-edge deltas in the shelf area and deep-water fans in the downdip slope area,and the strong source supply and relative sea level decline are the two key factors which control the development of this type of source-to-sink(S2S).The development of channels on the continental shelf edge is conducive to the formation of this type of S2S system even with weak source supply and high sea level.(2)Deltas that lack downdip fans:with the development of shelf edge deltas in shelf area,while deep water fans are not developed in the downdip slope area.The lack of“sources”and“channels”,and fluid transformation are the three main reasons for the formation of this type of S2S system.(3)Fans that lack updip coeval deltas:with the development of deep-water fans in continental slope area and the absence of updip coeval shelf edge deltas,which is jointly controlled by the coupling of fluid transformation at the shelf edge and the“channels”in the continental slope area. 展开更多
关键词 shelf-edge delta deep-water fan source-to-sink system Paleogene Zhuhai Formation Baiyun Sag Pearl River mouth Basin
下载PDF
Geometry and 3D seismic characterisation of post-rift normal faults in the Pearl River Mouth Basin,northern South China Sea
4
作者 Yuanhang Liu Jinwei Gao +2 位作者 Wanli Chen Jiliang Wang Umair Khan 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2024年第4期25-39,共15页
Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift ... Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift normal fault through detailed seismic interpretation and fault modeling.A total of 289 post-rift normal faults were identified in the study area and can be classified into four types:(1)isolated normal faults above the carbonate platform;(2)isolated normal faults cutting through the carbonate platform;(3)conjugate normal faults,and(4)connecting normal faults.Throw distribution analysis on the fault planes show that the vertical throw profiles of most normal fault exhibit flat-topped profiles.Isolated normal faults above the carbonate platform exhibit roughly concentric ellipses with maximum throw zones in the central section whereas the normal faults cutting through the carbonate platform miss the lowermost section due to the chaotic seismic reflections in the interior of the carbonate platform.The vertical throws of conjugate normal faults anomalously decrease toward their intersection region on the fault plane whereas the connecting normal faults present two maximum throw zones in the central section of the fault plane.According to the symmetric elliptical distribution model of fault throw,an estimation was made indicating that normal faults cutting through the carbonate platform extended downward between-1308 s and-1780 s(two-way travel time)in depth and may not penetrate the entire Liuhua carbonate platform.Moreover,it is observed that the distribution of karst caves on the top of the carbonate platform disaccord with those of hydrocarbon reservoirs and the post-rift normal faults cutting through the carbonate platform in the study area.We propose that these karst caves formed most probably by corrosive fluids derived from magmatic activities during the Dongsha event,rather than pore waters or hydrocarbons. 展开更多
关键词 Post-rift normal faults fault throw Karst caves Corrosive fluids Pearl River mouth Basin South China Sea
下载PDF
Characteristics of transfer zones under the influence of preexisting faults and regional stress transformation:Wenchang A subsag,Zhujiang River Mouth Basin,northern South China Sea
5
作者 Wei LI Mingyue CAO +7 位作者 Meifang MENG Caiwei FAN Hui LI Jia LI Wanqiu WU Ruiqing QIN Xingan LI Yiming LIU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第3期744-759,共16页
A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon... A transfer zone in rift basins preserves important information on regional tectonic evolution and plays significant roles in hydrocarbon accumulation.Based on the systematic analysis of 3D seismic data and hydrocarbon accumulation conditions,the geometry,kinematics,and reservoir control of a large synthetic overlapping transfer zone in the south of the Wenchang A subsag in the Zhujiang(Pearl)River Mouth basin were investigated.Results indicate that the development and evolution of the transfer zone was controlled by the interaction between pre-existing faults and regional stress transformation.The intense rifting of the main faults of the transfer zone controlled the development of source rocks and faultcontrolled slope break paleogeomorphology.The strike-slip overprint since the Oligocene is conducive to the formation of a large-scale fault-anticline trap,and the secondary faults in the transfer zone contribute to the hydrocarbon transportation.The conjugate intersection area of the NE-and NW-trending faults offers more opportunity for hydrocarbon migration and accumulation. 展开更多
关键词 transfer zone pre-existing fault regional stress transformation CENOZOIC Wenchang A Subsag Zhujiang(Pearl)River mouth Basin
下载PDF
Expression and clinical significance of pattern recognition receptor-associated genes in hand, foot and mouth disease
6
作者 Muqi Wang Huiling Deng +7 位作者 Yuan Chen Yikai Wang Yufeng Zhang Chenrui Liu Meng Zhang Ting Li Shuangsuo Dang Yaping Li 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2024年第4期173-183,I0001-I0003,共14页
Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR... Objective:To explore which pattern recognition receptors(PRRs)play a key role in the development of hand,foot,and mouth disease(HFMD)by analyzing PRR-associated genes.Methods:We conducted a comparative analysis of PRR-associated gene expression in human peripheral blood mononuclear cells(PBMCs)infected with enterovirus 71(EV-A71)which were derived from patients with HFMD of different severities and at different stages.A total of 30 PRR-associated genes were identified as significantly upregulated both over time and across different EV-A71 isolates.Subsequently,ELISA was employed to quantify the expression of the six most prominent genes among these 30 identified genes,specifically,BST2,IRF7,IFI16,TRIM21,MX1,and DDX58.Results:Compared with those at the recovery stage,the expression levels of BST2(P=0.027),IFI16(P=0.016),MX1(P=0.046)and DDX58(P=0.008)in the acute stage of infection were significantly upregulated,while no significant difference in the expression levels of IRF7(P=0.495)and TRIM21(P=0.071)was found between different stages of the disease.The expression levels of BST2,IRF7,IFI16 and MX1 were significantly higher in children infected with single pathogen than those infected with mixed pathogens,and BST2,IRF7,IFI16 and MX1 expression levels were significantly lower in coxsackie B virus(COXB)positive patients than the negative patients.Expression levels of one or more of BST2,IRF7,IFI16,TRIM21,MX1 and DDX58 genes were correlated with PCT levels,various white blood cell counts,and serum antibody levels that reflect disease course of HFMD.Aspartate aminotransferase was correlated with BST2,MX1 and DDX58 expression levels.Conclusions:PRR-associated genes likely initiate the immune response in patients at the acute stage of HFMD. 展开更多
关键词 Pattern recognition receptors(PRRs) Hand foot and mouth disease(HFMD) IMMUNE Enterovirus 71(EV-A71)
下载PDF
Transmission Dynamics and Optimal Control Strategies of a Hand-Foot-Mouth Disease Model with Treatment and Vaccination Interventions
7
作者 Jianping Wang Shenghua Zou Zhicai Guo 《Journal of Applied Mathematics and Physics》 2024年第6期2007-2019,共13页
In this article, the transmission dynamics of a Hand-Foot-Mouth disease model with treatment and vaccination interventions are studied. We calculated the basic reproduction number and proved the global stability of di... In this article, the transmission dynamics of a Hand-Foot-Mouth disease model with treatment and vaccination interventions are studied. We calculated the basic reproduction number and proved the global stability of disease-free equilibrium when R0 R0 > 1. Meanwhile, we obtained the optimal control strategies minimizing the cost of intervention and minimizing the infected person. We also give some numerical simulations to verify our theoretical results. 展开更多
关键词 Hand-Foot-mouth Disease Optimal Control Transmission Dynamic Vaccination Interventions
下载PDF
Tectonic-thermal history and hydrocarbon potential of the Pearl River Mouth Basin,northern South China Sea:Insights from borehole apatite fission-track thermochronology 被引量:1
8
作者 Xiao-yin Tang Shu-chun Yang Sheng-biao Hu 《China Geology》 CAS CSCD 2023年第3期429-442,共14页
The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evoluti... The Pearl River Mouth Basin(PRMB)is one of the most petroliferous basins on the northern margin of the South China Sea.Knowledge of the thermal history of the PRMB is significant for understanding its tectonic evolution and for unraveling its poorly studied source-rock maturation history.Our investigations in this study are based on apatite fission-track(AFT)thermochronology analysis of 12 cutting samples from 4 boreholes.Both AFT ages and length data suggested that the PRMB has experienced quite complicated thermal evolution.Thermal history modeling results unraveled four successive events of heating separated by three stages of cooling since the early Middle Eocene.The cooling events occurred approximately in the Late Eocene,early Oligocene,and the Late Miocene,possibly attributed to the Zhuqiong II Event,Nanhai Event,and Dongsha Event,respectively.The erosion amount during the first cooling stage is roughly estimated to be about 455-712 m,with an erosion rate of 0.08-0.12 mm/a.The second erosion-driven cooling is stronger than the first one,with an erosion amount of about 747-814 m and an erosion rate between about 0.13-0.21 mm/a.The erosion amount calculated related to the third cooling event varies from 800 m to 3419 m,which is speculative due to the possible influence of the magmatic activity. 展开更多
关键词 Oil and gas Hydrocarbon potential Apatite fission-track Tectonic-thermal evolution Thermal history modeling Cooling event Heating event Marine geological survey engineering Erosion amount and rate Oil-gas exploration engineering Pearl River mouth Basin The South China Sea
下载PDF
Computational Investigation of Hand Foot Mouth Disease Dynamics with Fuzziness
9
作者 Dumitru Baleanu Fazal Dayan +3 位作者 Nauman Ahmed Muhammad Rafiq Ali Raza Muhammad Ozair Ahmad 《Computers, Materials & Continua》 SCIE EI 2023年第5期4175-4189,共15页
The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The par... The first major outbreak of the severely complicated hand,foot and mouth disease(HFMD),primarily caused by enterovirus 71,was reported in Taiwan in 1998.HFMD surveillance is needed to assess the spread of HFMD.The parameters we use in mathematical models are usually classical mathematical parameters,called crisp parameters,which are taken for granted.But any biological or physical phenomenon is best explained by uncertainty.To represent a realistic situation in any mathematical model,fuzzy parameters can be very useful.Many articles have been published on how to control and prevent HFMD from the perspective of public health and statistical modeling.However,few works use fuzzy theory in building models to simulateHFMDdynamics.In this context,we examined anHFMD model with fuzzy parameters.A Non Standard Finite Difference(NSFD)scheme is developed to solve the model.The developed technique retains essential properties such as positivity and dynamic consistency.Numerical simulations are presented to support the analytical results.The convergence and consistency of the proposed method are also discussed.The proposed method converges unconditionally while the many classical methods in the literature do not possess this property.In this regard,our proposed method can be considered as a reliable tool for studying the dynamics of HFMD. 展开更多
关键词 Hand foot mouth disease fuzzy parameters NSFD scheme CONVERGENCE CONSISTENCY
下载PDF
Construction of Plant Expression Vector for Hand,Foot and Mouth Virus EV71-VP1 Gene and Its Expression in Tomato
10
作者 Wang Mei-liang Zhao Yue +5 位作者 Wang Yu-dan Li Xin-zhi Zhang Yao Chen Xiu-ling Qiu You-wen Wang Ao-xue 《Journal of Northeast Agricultural University(English Edition)》 2023年第4期53-62,共10页
EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was... EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been isolated.The tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid protein.Using molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were obtained.Five transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA level.The experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future. 展开更多
关键词 HAND foot and mouth disease(HFMD) EV71-VP1 TOMATO plant transgenic vaccine vector construction
下载PDF
Analysis of the Effect of Mindfulness Meditation on Patients with Burning Mouth Syndrome
11
作者 Yanqing Feng Mengjun Huang Fengqiao Lin 《Journal of Clinical and Nursing Research》 2023年第6期36-40,共5页
Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 wer... Objective:To explore the effect of mindfulness meditation on patients with burning mouth syndrome.Methods:60 patients with burning mouth syndrome in our hospital who were treated from January 2021 to December 2022 were selected for this study.The patients were divided into two groups of thirty cases each using the randomized numerical table method.The observation underwent psychological intervention and mindfulness meditation training,while the control group only received symptomatic care.The condition of the patients of both groups was observed and compared.Results:Upon receiving treatment,the patients in the observation group had lower Hamilton Anxiety(HAM-A)scores,and Hamilton Depression(HAMD)scores compared to the control group(P<0.05).The visual analog scale(VAS)scores of the observation group were also lower than those of the control group(P<0.05).Moreover,the efficacy of the nursing intervention in the observation group was higher than that of the control group(P<0.05).Conclusion:Psychological intervention and mindfulness meditation training can effectively improve the clinical symptoms of patients with burning mouth syndrome.Therefore,this treatment method should be popularized. 展开更多
关键词 Burning mouth syndrome Psychological intervention Mindfulness meditation training
下载PDF
珠江口盆地白云凹陷东部古近系文昌组沉积演化特征 被引量:1
12
作者 刘太勋 孙丰春 +4 位作者 彭光荣 汪旭东 孙辉 解斌 苏兆佳 《中国石油大学学报(自然科学版)》 EI CAS CSCD 北大核心 2024年第1期77-90,共14页
充分利用新三维地震资料,结合测井以及录井资料,对珠江口盆地白云凹陷古近系文昌组层序地层、沉积特征及沉积演化进行研究,重新建立文昌组三级层序格架内的沉积体系演化过程。结果表明:白云凹陷东部文昌组自下而上共划分为5套三级层序,... 充分利用新三维地震资料,结合测井以及录井资料,对珠江口盆地白云凹陷古近系文昌组层序地层、沉积特征及沉积演化进行研究,重新建立文昌组三级层序格架内的沉积体系演化过程。结果表明:白云凹陷东部文昌组自下而上共划分为5套三级层序,分别对应于初始裂陷期(WCSQ1)、拆离裂陷早期(WCSQ2,WCSQ3)、拆离裂陷晚期(WCSQ4,WCSQ5)3个构造演化阶段;初始裂陷期为多个孤立的箕状半地堑,以发育缓坡轴向物源辫状河三角洲、陡坡近源扇三角洲和滨浅湖沉积为特征;拆离裂陷早期断裂活动强烈,湖盆迅速扩张,半深湖—深湖局限发育,沉积中心北移,北部番禺低隆起发育大型辫状河三角洲沉积;拆离裂陷晚期断裂活动减弱,湖盆逐渐萎缩,沉积中心稳定在白云凹陷中部,主要发育小型辫状河三角洲沉积,滨浅湖发育;大型辫状河三角洲及扇三角洲砂体是白云凹陷东部的有利储集体。 展开更多
关键词 沉积相 沉积模式 文昌组 白云凹陷 珠江口盆地
下载PDF
基于双特异纳米抗体FMDV血凝检测方法的建立
13
作者 杨利 张浩明 +3 位作者 乔绪稳 陈瑾 郑其升 程海卫 《江苏农业学报》 CSCD 北大核心 2024年第3期514-521,共8页
本研究利用基因工程方法将抗口蹄疫病毒(FMDV)纳米抗体Nb205和抗鸡醛化红细胞(cRBC)纳米抗体NbRBC48的基因片段连接,构建双特异纳米抗体Nb205-48,其相对分子质量为3.5×10^(4),能同时与FMDV和cRBC反应。利用Nb205-48成功建立了可用... 本研究利用基因工程方法将抗口蹄疫病毒(FMDV)纳米抗体Nb205和抗鸡醛化红细胞(cRBC)纳米抗体NbRBC48的基因片段连接,构建双特异纳米抗体Nb205-48,其相对分子质量为3.5×10^(4),能同时与FMDV和cRBC反应。利用Nb205-48成功建立了可用于FMDV抗原检测的血凝方法,该方法检测灵敏度为1μg/ml,与其他病毒无交叉反应,且批内和批间重复性好。分别采用血凝法和夹心ELISA方法对3批次FMDV抗原进行了检测,二者检测结果基本吻合。本研究建立了基于双特异纳米抗体Nb205-48的血凝检测方法,该方法具有较好的灵敏度、特异性和重复性,且简便、快捷、成本低,其检测结果与传统的FMDV定量检测方法的检测结果相关性好,为检测口蹄疫疫苗生产过程中FMDV抗原的含量提供了新方法。 展开更多
关键词 口蹄疫病毒 双特异性纳米抗体 血凝检测法
下载PDF
嵌合流行毒株G-H环基因重组口蹄疫病毒的拯救及其免疫原性分析
14
作者 李平花 黄书伦 +11 位作者 张克强 刘锋 孙普 李冬 包慧芳 曹轶梅 白兴文 马雪青 李坤 袁红 刘在新 卢曾军 《畜牧兽医学报》 CAS CSCD 北大核心 2024年第11期5222-5229,共8页
为了研究能有效免疫防控当前流行的O型3个拓扑型口蹄疫病毒(foot-and-mouth disease virus,FMDV)的疫苗候选株,本研究利用FMDV反向遗传操作技术,通过基因替换,构建同时含当前流行的O型三个拓扑型病毒株结构蛋白基因的重组全长克隆,转染... 为了研究能有效免疫防控当前流行的O型3个拓扑型口蹄疫病毒(foot-and-mouth disease virus,FMDV)的疫苗候选株,本研究利用FMDV反向遗传操作技术,通过基因替换,构建同时含当前流行的O型三个拓扑型病毒株结构蛋白基因的重组全长克隆,转染表达T7RNA聚合酶的细胞后拯救重组FMDV,分析结构蛋白基因的重构对病毒生物学特性的影响;拯救病毒制备灭活疫苗,免疫猪和牛,用体外中和试验初步研究其作为O型FMD疫苗候选株的潜力。结果表明FMD流行毒株结构蛋白VP1 G-H环基因的替换没有明显影响重组病毒的噬斑表型和复制能力,但不同FMDV G-H环抗原表位对猪诱导机体产生交叉中和抗体水平影响较大,对牛诱导机体产生交叉中和抗体的影响较小,表明FMDV G-H抗原表位是猪免疫优势的表位。本研究为未来FMDV疫苗的设计提供了重要参考。 展开更多
关键词 G-H环 重组口蹄疫病毒 拯救 免疫原性分析
下载PDF
珠江口盆地潜山储层地质力学及压裂参数优化研究 被引量:1
15
作者 肖阳 马中慧 +3 位作者 刘书云 韩晨辉 夏瀚涛 张进源 《科学技术与工程》 北大核心 2024年第4期1392-1401,共10页
珠江口盆地珠一坳陷惠州27-1井区作为潜山油气储层,压裂层段厚度大、层数多、非均质性强、压裂改造难度大,对于人工裂缝的在潜山储层中的拓展延伸规律认识困难。针对此问题,首先建立动态参数测井解释模型,并与静态岩石力学数据协同校正... 珠江口盆地珠一坳陷惠州27-1井区作为潜山油气储层,压裂层段厚度大、层数多、非均质性强、压裂改造难度大,对于人工裂缝的在潜山储层中的拓展延伸规律认识困难。针对此问题,首先建立动态参数测井解释模型,并与静态岩石力学数据协同校正,建立一维岩石力学剖面;结合深部成岩理论,建立三维地质力学模型,真实模拟研究区块成岩环境;最后基于三维地质力学模型,研究排量、液量、砂比、前置液占比、射孔层位厚度等参数对裂缝纵向和平面延伸规律的影响,优化压裂设计。结果表明:惠州27-1区块,杨氏模量平均值为45.47 GPa,最大水平主应力平均值为100.91 MPa,最小水平主应力平均值为80.98 MPa,泊松比平均值为0.30。排量6~7 m^(3)/min、液量600~700 m^(3)、砂比10%~15%、前置液比例50%~60%、射孔厚度8~11 m为最优施工参数,为现场施工提供指导意见。 展开更多
关键词 珠江口盆地 潜山储层 地质力学 压裂参数优化 数值模拟
下载PDF
珠江口盆地开平南大型深水深层油田发现与认识创新 被引量:5
16
作者 徐长贵 高阳东 +4 位作者 刘军 彭光荣 陈兆明 姜大朋 蔡俊杰 《中国海上油气》 CAS CSCD 北大核心 2024年第1期1-13,共13页
开平凹陷是珠江口盆地地质条件最复杂的凹陷之一,也是重要的深水油气勘探区,由于洼陷结构、烃源分布及成藏规律认识不清,长期制约勘探进程。基于三维地震资料精细解释,开展成盆机制、烃源发育、源汇体系及成藏主控因素等研究,近期在开... 开平凹陷是珠江口盆地地质条件最复杂的凹陷之一,也是重要的深水油气勘探区,由于洼陷结构、烃源分布及成藏规律认识不清,长期制约勘探进程。基于三维地震资料精细解释,开展成盆机制、烃源发育、源汇体系及成藏主控因素等研究,近期在开平南获得了大型轻质油田勘探发现。为进一步指导勘探,分析了开平凹陷油气成藏条件,总结了油气成藏模式。结果表明:(1)开平凹陷是南海北部强伸展陆缘背景下发育的“拆离-核杂岩”型凹陷,裂陷期经历“断陷—拆离—断拗”3幕演化过程;(2)开平凹陷发育文昌组优质湖相烃源岩,分布范围广、生烃潜力大,总体处于成熟至高成熟阶段;(3)开平凹陷油气运移受“源-断-脊”耦合控制,开平凹陷南部3大鼻状构造带是油气有利汇聚区,总体具有“近源强势充注-断脊差异运聚-晚期快速成藏”的模式。开平南古近系大型轻质油田的发现实现了开平凹陷勘探重大突破,开辟了珠江口盆地深水区油气勘探的新区、新领域,同时为“拆离-核杂岩”型含油气盆地的勘探提供了借鉴。 展开更多
关键词 珠江口盆地 开平凹陷 深水 深层 古近系 开平南 开平11-4 开平18-1
下载PDF
2020-2022年丽水市儿童手足口病流行特征及病原谱分析
17
作者 陈秀英 叶碧峰 +2 位作者 王晓光 卢愉中 叶夏良 《中国当代医药》 CAS 2024年第6期120-123,共4页
目的分析2020—2022年丽水市儿童手足口病(HFMD)流行病学特征和病原学监测结果,为HFMD防控提供科学依据。方法通过传染病报告信息管理系统收集丽水市HFMD发病资料,对流行特征进行分析,同时采用荧光定量PCR技术对收集的1558例标本开展病... 目的分析2020—2022年丽水市儿童手足口病(HFMD)流行病学特征和病原学监测结果,为HFMD防控提供科学依据。方法通过传染病报告信息管理系统收集丽水市HFMD发病资料,对流行特征进行分析,同时采用荧光定量PCR技术对收集的1558例标本开展病原学检测,探究2020—2022年HFMD肠道病毒病原谱的动态变化。结果丽水市儿童HFMD发病年龄主要集中在0~5岁,2020年发病主要集中在9月至12月,2021—2022年主要集中在4月至7月份。2020—2022年间丽水市累计报告HFMD病例为13611例,在1558例病原学标本中检出肠道病毒核酸阳性1032份,总阳性率为66.24%。其中肠道病毒A组71型(EV71)5份,总阳性率为0.32%;柯萨奇病毒A组16型(CoxA16)322份,总阳性率为20.67%;柯萨奇病毒A组6型(CoxA6)型494份,总阳性率为31.71%;柯萨奇病毒A组10型(CoxA10型)63份,总阳性率为4.04%;其他型别148份,总阳性率9.5%。2020—2022年间,EV71仅在2021年检出5例;CoxA16型阳性率分别为2.44%、21.54%和36.42%,2022年1月至8月和11月至12月为检出高峰;CoxA6型阳性率分别为49.78%、33.44%和12.76%,2020年8月至12月为检出高峰;CoxA10型阳性率只在2020年和2021年有检出,分别为4.00%、7.23%。结论除2020年HFMD流行月份整体延后外,其余年份流行特征类似于往年。2020—2022年丽水市HFMD的主要肠道病毒型别为CoxA16和CoxA6型,期间CoxA6型的优势已逐步被CoxA16取代。CoxA16为下一步防控工作的重点。 展开更多
关键词 手足口病 流行特征 病原谱分析
下载PDF
口腔黏膜下纤维性变的治疗研究进展
18
作者 陈灵 杜永秀 徐普 《河北医药》 CAS 2024年第17期2675-2679,2685,共6页
口腔黏膜下纤维性变(OSF)是一种慢性、潜伏性疾病,被归类为口腔潜在恶性疾病,是一个全球性和区域性的公共卫生问题。它被认为是一种多因素疾病,如咀嚼槟榔、微量元素紊乱和遗传易感性等。本文回顾总结近年来国内外治疗OSF的各种方法,包... 口腔黏膜下纤维性变(OSF)是一种慢性、潜伏性疾病,被归类为口腔潜在恶性疾病,是一个全球性和区域性的公共卫生问题。它被认为是一种多因素疾病,如咀嚼槟榔、微量元素紊乱和遗传易感性等。本文回顾总结近年来国内外治疗OSF的各种方法,包括药物治疗、手术治疗、物理治疗等,以上治疗方法主要是减轻疾病的体征和症状,尚缺乏单一有效的治疗方法。此外,有部分研究者提出新的治疗方法-干细胞治疗,但是观察病例量较少,疗效和安全性有待进一步研究。 展开更多
关键词 口腔黏膜下纤维性变 治疗 张口受限 干细胞治疗
下载PDF
天津市手足口病防控知识科普宣讲活动及效果评价 被引量:1
19
作者 高璐 程思 +2 位作者 杨杰 卢婷婷 李琳 《天津科技》 2024年第S01期75-78,共4页
2023年6—10月,对天津市16个区进行分层抽样,抽取4个宣传地区及其24所托幼机构,对849位幼儿家长、705名校(园)医、129名社区医生开展手足口病防控知识科普宣讲活动,宣传形式包括家长课堂宣讲,校医、社区医生现场活动和答疑解惑,以科普... 2023年6—10月,对天津市16个区进行分层抽样,抽取4个宣传地区及其24所托幼机构,对849位幼儿家长、705名校(园)医、129名社区医生开展手足口病防控知识科普宣讲活动,宣传形式包括家长课堂宣讲,校医、社区医生现场活动和答疑解惑,以科普文章制作转发、视频播放等形式传播,并针对现场宣讲活动人群开展问卷调查。科普宣讲活动后,手足口病日常预防知识知晓率及防控行为率明显升高。提出对于需要多次强化或不易记忆的日常防护知识,可采用科普宣讲和短视频循环播放相结合的方式。 展开更多
关键词 手足口病防控 宣讲活动 评价
下载PDF
地形地貌变化对调水调沙期间黄河口沉积格局的影响
20
作者 毕乃双 时义睿 +3 位作者 葛春海 巴旗 吴晓 王厚杰 《海洋地质与第四纪地质》 CAS CSCD 北大核心 2024年第5期1-14,共14页
自2002年实施调水调沙以来,由于入海径流、沉积物的通量和组成发生变化,黄河现行河口三角洲叶瓣不断向海淤积造陆,水下三角洲坡度变陡。地形地貌变化对调水调沙期间入海泥沙沉积格局的影响成为黄河口研究的重要科学问题。本文基于Delft3... 自2002年实施调水调沙以来,由于入海径流、沉积物的通量和组成发生变化,黄河现行河口三角洲叶瓣不断向海淤积造陆,水下三角洲坡度变陡。地形地貌变化对调水调沙期间入海泥沙沉积格局的影响成为黄河口研究的重要科学问题。本文基于Delft3D模型系统,利用岸线、水深和河流水沙数据构建三维水沙数值模型,对2002年、2008年、2014年和2019年调水调沙期间现行河口近岸海域泥沙的输运和沉积过程进行模拟。结果表明,随着水深、岸线的变化,黄河口近岸海域动力环境增强,泥沙的横向输运增强,纵向输运相应减弱;进而导致黄河入海泥沙堆积体的横向长度增加约30%,纵向长度减小约27%,厚度、形态也相应变化。本研究揭示了地形地貌变化条件下,黄河调水调沙期间入海泥沙在河口的沉积格局及动力机制,对深入理解黄河口近岸海域水动力-地貌耦合系统有重要参考价值。 展开更多
关键词 地形地貌 沉积格局 调水调沙 Delft3D模型 黄河口
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部