目的评估MALDI-TOF MS鉴定酵母菌的能力。方法分别用VITEK MS和API ID 32C系统同时鉴定387株临床分离酵母菌。以ITS测序结果作为比较标准。比较MALDI-TOF MS、API ID 32C结果准确率。结果 VITEK MS鉴定酵母菌准确率为97.7%,API ID 32C...目的评估MALDI-TOF MS鉴定酵母菌的能力。方法分别用VITEK MS和API ID 32C系统同时鉴定387株临床分离酵母菌。以ITS测序结果作为比较标准。比较MALDI-TOF MS、API ID 32C结果准确率。结果 VITEK MS鉴定酵母菌准确率为97.7%,API ID 32C鉴定准确率为93.0%,差异有统计学意义(χ~2=9.439,P=0.002);MALDITOF与ITS测序相比,差异无统计学意义。结论 MALDITOF可对临床常见酵母菌快速、准确鉴定到种。展开更多
To solve the problem that soybean has narrow genetic base, we constructed a series of male sterile recurrent selection populations, and studied the effects of quality improvement and practical value. An LD-base popula...To solve the problem that soybean has narrow genetic base, we constructed a series of male sterile recurrent selection populations, and studied the effects of quality improvement and practical value. An LD-base population, which fits to our ecology type was constructed by 6 years＇ gene enrichment through the introduction of new genes from 23 local varieties and recurrent selection. The LD-base populations were then improved by making crosses with high protein and high oil genotypes. As a result we obtained a high protein sub-population （db） and a high oil sub-population （gy）, For the db sub-population, the protein content is 1.18% higher than the base population, 22.38% of the individuals contain 45% or more of protein, which is 10.99% higher than the base population. For the gy sub-population, oil content is 0.24% higher than the base population. Individuals with oil content of 20% or more are 11.05% higher than the base population. The quantitative characters such as flowering date, mature date, pod habit, and hilum color, etc., all showed wide range of separation, and the segregation ratio approached balance. The c.v. of branch number of ms1 recurrent population （72.8%） is higher than general cross-population （57.3%）, and the c.v. of 100 seed weight of ms1 （18.1%） is higher than general cross population （16.5%）, the coefficient of variation of plant height, pods per plant, and seeds per pod were not significantly different. It was demonstrated in this paper that the quality character of ms 1 male sterile recurrent selection population was improved by adding new genes. And the segregation of other characters widened, making the populations suitable for the objective of soybean breeding. In this paper, we also discussed the breeding method, key technology, and selection effect of soybean ms 1 population.展开更多
文摘目的评估MALDI-TOF MS鉴定酵母菌的能力。方法分别用VITEK MS和API ID 32C系统同时鉴定387株临床分离酵母菌。以ITS测序结果作为比较标准。比较MALDI-TOF MS、API ID 32C结果准确率。结果 VITEK MS鉴定酵母菌准确率为97.7%,API ID 32C鉴定准确率为93.0%,差异有统计学意义(χ~2=9.439,P=0.002);MALDITOF与ITS测序相比,差异无统计学意义。结论 MALDITOF可对临床常见酵母菌快速、准确鉴定到种。
文摘To solve the problem that soybean has narrow genetic base, we constructed a series of male sterile recurrent selection populations, and studied the effects of quality improvement and practical value. An LD-base population, which fits to our ecology type was constructed by 6 years＇ gene enrichment through the introduction of new genes from 23 local varieties and recurrent selection. The LD-base populations were then improved by making crosses with high protein and high oil genotypes. As a result we obtained a high protein sub-population （db） and a high oil sub-population （gy）, For the db sub-population, the protein content is 1.18% higher than the base population, 22.38% of the individuals contain 45% or more of protein, which is 10.99% higher than the base population. For the gy sub-population, oil content is 0.24% higher than the base population. Individuals with oil content of 20% or more are 11.05% higher than the base population. The quantitative characters such as flowering date, mature date, pod habit, and hilum color, etc., all showed wide range of separation, and the segregation ratio approached balance. The c.v. of branch number of ms1 recurrent population （72.8%） is higher than general cross-population （57.3%）, and the c.v. of 100 seed weight of ms1 （18.1%） is higher than general cross population （16.5%）, the coefficient of variation of plant height, pods per plant, and seeds per pod were not significantly different. It was demonstrated in this paper that the quality character of ms 1 male sterile recurrent selection population was improved by adding new genes. And the segregation of other characters widened, making the populations suitable for the objective of soybean breeding. In this paper, we also discussed the breeding method, key technology, and selection effect of soybean ms 1 population.
