To investigate the genetic variation and population structure of Pacific herring in the Yellow Sea and the genetic differentiation between the Yellow Sea and the Sea of Japan, fragments of 479-bp mitochondrial DNA con...To investigate the genetic variation and population structure of Pacific herring in the Yellow Sea and the genetic differentiation between the Yellow Sea and the Sea of Japan, fragments of 479-bp mitochondrial DNA control region were sequenced for 110 individuals collected from three different periods in the Yellow Sea and one locality in the Sea of Japan. High haplotype diversity and moderate nucleotide diversity were observed in Pacific herring. AMOVA and exact test of population differentiation showed no significant genetic differentiations among the three populations of the Yellow Sea and suggested the populations can be treated as a single panmictic stock in the Yellow Sea. However, a large and significant genetic differentiation （ФST=0.11; P=0.00） was detected between the populations in the Yellow Sea and the Sea of Japan. The high sea water temperature in the Tsushima Strait was thought a barrier to block the gene exchange between populations of the two sea areas. The neutrality tests and mismatch distribution indicated recent population expansion in Pacific herring.展开更多
Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood o...Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identifi ed. The genetic diversity was calculated to be 0.9691, and the genetic identity was calculated to be 0.0406. Conclusion There are some particul ar polymorphism sites in Chinese YUGU ethnic group. The results suggest that seq uence polymorphism from np16091-16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.展开更多
Mitochondrial DNA sequences of the entire control region and three coding regions were analyzed in 108 unrelated individuals from three regions of Bangladesh. Sequence evaluation was performed with validated primers a...Mitochondrial DNA sequences of the entire control region and three coding regions were analyzed in 108 unrelated individuals from three regions of Bangladesh. Sequence evaluation was performed with validated primers and combined sequence comparison led to the identification of 14 different haplotypes characterized by 37 variable polymorphic sites. The Bangladeshi sequences exhibited high variations and low random match probability, indicating for forensic application. The mean pairwise difference between individual was 9.698 ± 1.8658 nucleotides (95% CI 9.67 - 9.69), compared to a mean pairwise difference of 9.890 ± 4.189 nucleotides reported from Northeast Asia and suggested significant differences in the mtDNA composition of the various populations. The sequence diversity of 108 Bangladeshi Bengali samples (n = 216 chromosomes) was estimated to be 0.8475 ± 0.13406. This study first time reports that the comparison of closely related mtDNA sequences can be very useful for improving mtDNA database quality, as well as provide haplotype information for forensic study in mainstream population of Bangladesh.展开更多
基金Supported by the National Natural Science Foundation of China(No. 31061160187)Special Fund for Agro-scientific Research in the Public Interest (No. 200903005)Ocean University of China Students Innovation Trainning Program
文摘To investigate the genetic variation and population structure of Pacific herring in the Yellow Sea and the genetic differentiation between the Yellow Sea and the Sea of Japan, fragments of 479-bp mitochondrial DNA control region were sequenced for 110 individuals collected from three different periods in the Yellow Sea and one locality in the Sea of Japan. High haplotype diversity and moderate nucleotide diversity were observed in Pacific herring. AMOVA and exact test of population differentiation showed no significant genetic differentiations among the three populations of the Yellow Sea and suggested the populations can be treated as a single panmictic stock in the Yellow Sea. However, a large and significant genetic differentiation （ФST=0.11; P=0.00） was detected between the populations in the Yellow Sea and the Sea of Japan. The high sea water temperature in the Tsushima Strait was thought a barrier to block the gene exchange between populations of the two sea areas. The neutrality tests and mismatch distribution indicated recent population expansion in Pacific herring.
文摘Objective To investigate the mitochondrial DN A sequence polymorphism sites in Chinese YUGU ethnic group and to provide basic da ta used in forensic purpose. Methods Genomic DNA was extracted from the hole blood of 100 unrelated individuals of Chinese YUGU ethnic group by standard chelex-100 method. The sequence polymorphism sites was determined by PCR amplification and direct sequencing. Results 54 polymorphic sites were noted in mtDNA np16091-16418 region, and 46 haplotypes were identifi ed. The genetic diversity was calculated to be 0.9691, and the genetic identity was calculated to be 0.0406. Conclusion There are some particul ar polymorphism sites in Chinese YUGU ethnic group. The results suggest that seq uence polymorphism from np16091-16418 in human mitochondrial DNA can be used as a biological marker for forensic identity.
文摘Mitochondrial DNA sequences of the entire control region and three coding regions were analyzed in 108 unrelated individuals from three regions of Bangladesh. Sequence evaluation was performed with validated primers and combined sequence comparison led to the identification of 14 different haplotypes characterized by 37 variable polymorphic sites. The Bangladeshi sequences exhibited high variations and low random match probability, indicating for forensic application. The mean pairwise difference between individual was 9.698 ± 1.8658 nucleotides (95% CI 9.67 - 9.69), compared to a mean pairwise difference of 9.890 ± 4.189 nucleotides reported from Northeast Asia and suggested significant differences in the mtDNA composition of the various populations. The sequence diversity of 108 Bangladeshi Bengali samples (n = 216 chromosomes) was estimated to be 0.8475 ± 0.13406. This study first time reports that the comparison of closely related mtDNA sequences can be very useful for improving mtDNA database quality, as well as provide haplotype information for forensic study in mainstream population of Bangladesh.
