This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11...This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.展开更多
文摘[目的]研究庆阳驴养殖群体的遗传多样性与母系起源,了解其遗传信息,为保护庆阳驴种质资源、选育和遗传改良工作提供理论依据。[方法]随机选取133头庆阳驴,对其线粒体DNA(mitochondrial DNA,mtDNA)D-loop区序列进行PCR扩增、测序及比对,并探讨庆阳驴的遗传多样性与母系起源。[结果]在获得的520 bp D-loop碱基序列中,AT含量(57.3%)高于GC含量(42.8%),表现出碱基的偏倚性;检测到38个变异位点,包含8个碱基对的转换;其核苷酸多样性(Pi)、单倍型多样性(Hd)、平均核苷酸差异(K)分别为0.01591、0.895和8.274,与欧洲家驴和中国家驴研究的平均值相比较低,说明该驴品种核苷酸变异较为贫乏。庆阳驴mtDNA D-loop区存在35个单倍型,单倍型之间的遗传距离为0.002~0.042。系统进化结果显示,庆阳驴存在2个线粒体支系,表明其具有2个母系起源,且遗传距离表明,庆阳驴与克罗地亚家驴之间的遗传距离较近。[结论]本研究从分子水平初步揭示庆阳驴核苷酸变异比较贫乏,杂交程度高,mtDNA遗传多态性正逐步丧失,应加强庆阳驴品种的遗传资源保护工作。
文摘This study analyzed the mitochondrial DNA D-loop hypervariable region 601 bp sequence in 12 Eutamias sibiricus from Heilongjiang area. The result showed that the average contents of A, T, G and C were 33.2%, 30.5%, 11.8% and 24.5% respectively, the A＋T content （63.7%） was obviously higher than the G＋C content （36.3%）. Thirty-six, mutation （approximately 6.0%） sites were found and 9 haplotypes were defined. The mutations types, including transition, transversion and deletion were all found in the detected mtDNA D-loop regions, most of which was transition. The average nucleotide mutational ratio was 1.22%. The nucleotide mutation sites affected the restriction site appearance or disappearance of the restriction site. The research on mtDNA D-loop is focused on the domestic animals and there is no report on Eutamias sibiricus, This study analyzed the mitochondrial DNA D-loop hypervariable in Eutamias sibiricus so as to provide some useful informations for related research in the future.
文摘为更好地对长顺绿壳蛋鸡进行品种保护和利用,并从母系遗传角度深入阐明长顺绿壳蛋鸡的群体遗传背景,利用PCR直接测序法,测定了114只长顺绿壳蛋鸡的线粒体DNA(mt DNA)D-loop第1高变区568 bp片段序列。结果表明:在所分析的长顺绿壳蛋鸡mt DNA D-loop区序列中,A、C、G、T平均比例为27.1%、29.5%、13.4%、30.0%;共检测到核苷酸变异位点37个,核苷酸多样度为0.0111,24种单倍型,单倍型多样度为0.845,表明长顺绿壳蛋鸡遗传多样性较丰富,具有较好的选育潜力。聚类分析结果表明,长顺绿壳蛋鸡与红原鸡亲缘关系较近。
