Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of e...Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of expression of TSLP in nasal epithelial cells （NECs） of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR （n=22） and nonallergic controls （n=11） during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale （VAS）. In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization （ISH） was performed to test the messenger RNA （mRNA） of TSLP in the NECs. Furthermore, immunohistochemical staining （IHC） was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group （P 〈0.05）. In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score （r=0.598; P 〈0.05） and nasal eosinophils count （r=0.702; P 〈0.05）, but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local atopical responses of AR. In the future, the TSLP has the potential to be one of the most important molecular markers for AR diagnoses and assessment.展开更多
目的 Pru p 3是桃主要致敏蛋白,目前尚未见桃Pru p 3 T细胞表位在我国桃过敏反应中的研究,本研究探讨桃主要致敏蛋白Pru p 3 T细胞表位。方法纳入蒿花粉相关桃过敏患者20例和健康对照者10人,采集受检者的外周血单个核细胞(PBMC),将PBMC...目的 Pru p 3是桃主要致敏蛋白,目前尚未见桃Pru p 3 T细胞表位在我国桃过敏反应中的研究,本研究探讨桃主要致敏蛋白Pru p 3 T细胞表位。方法纳入蒿花粉相关桃过敏患者20例和健康对照者10人,采集受检者的外周血单个核细胞(PBMC),将PBMC分别与8条Pru p 3来源的肽段共同孵育,比较桃过敏患者和健康对照者PBMC受各肽段刺激后的增生水平,分析不同肽段的变应原性。结果肽段Pru p 367-82对桃过敏患者PBMC的活化程度最高,20例桃过敏患者中对该肽段刺激指数(SI)阳性者14例,占70%; Pru p 323-38对桃过敏患者PBMC的活化率为55%(11/20)。Pru p 367-82与Art v 390-105氨基酸序列的一致率为81%。结论 Pru p 323-38和Pru p 367-82是引起我国桃过敏反应的主要T细胞表位,Pru p 367-82可能是参与蒿花粉-桃交叉反应的T细胞表位。展开更多
文摘Background Excessive expression of thymic stromal lymphopoietin （TSLP） has been demonstrated in asthmatic airway epithelia and in nasal epithelia from animal models of allergic rhinitis （AR）, but the evidence of expression of TSLP in nasal epithelial cells （NECs） of patients with AR is lacking. We aimed to investigate the expression of TSLP in NECs of patients with mugwort sensitive-seasonal AR and determine whether it is associated with severity of symptoms and the number of infiltrated eosinophils in nasal mucosa. Methods NECs specimens were obtained by scraping with plastic curettes from the nasal inferior turbinates of patients with mugwort pollen sensitive-seasonal AR （n=22） and nonallergic controls （n=11） during last peak mugwort pollen season. The severity of nasal symptom was assessed using a Visual Analog Scale （VAS）. In addition, serum mugwort pollen IgE levels were tested from each patient. In situ hybridization （ISH） was performed to test the messenger RNA （mRNA） of TSLP in the NECs. Furthermore, immunohistochemical staining （IHC） was scored to evaluate the expression of TSLP and eosinophil cell count was made by May-Grunwald/Giemsa staining. The correlation between expression of TSLP and all other parameters was analyzed in this study. Results The mRNA level of TSLP was significantly increased in NECs of patients with AR compared with the nonallergic control group （P 〈0.05）. In addition, IHC results showed that expression of TSLP in NECs from patients with AR was up-regulated which was correlated with VAS score （r=0.598; P 〈0.05） and nasal eosinophils count （r=0.702; P 〈0.05）, but it was unrelated with mugwort pollen specific IgE level. Conclusions These preliminary findings indicate a potential relationship between TSLP expression, severity of symptoms and nasal eosinophils count in pathogenesis of AR, but TSLP expression did not correlate with mugwort pollen specific IgE level. The elevated expression of TSLP might play a critical role in local atopical responses of AR. In the future, the TSLP has the potential to be one of the most important molecular markers for AR diagnoses and assessment.
文摘目的 Pru p 3是桃主要致敏蛋白,目前尚未见桃Pru p 3 T细胞表位在我国桃过敏反应中的研究,本研究探讨桃主要致敏蛋白Pru p 3 T细胞表位。方法纳入蒿花粉相关桃过敏患者20例和健康对照者10人,采集受检者的外周血单个核细胞(PBMC),将PBMC分别与8条Pru p 3来源的肽段共同孵育,比较桃过敏患者和健康对照者PBMC受各肽段刺激后的增生水平,分析不同肽段的变应原性。结果肽段Pru p 367-82对桃过敏患者PBMC的活化程度最高,20例桃过敏患者中对该肽段刺激指数(SI)阳性者14例,占70%; Pru p 323-38对桃过敏患者PBMC的活化率为55%(11/20)。Pru p 367-82与Art v 390-105氨基酸序列的一致率为81%。结论 Pru p 323-38和Pru p 367-82是引起我国桃过敏反应的主要T细胞表位,Pru p 367-82可能是参与蒿花粉-桃交叉反应的T细胞表位。
