野蔷薇种子快速催芽技术研究

[目的]为了解决野蔷薇种子休眠期长、发芽不整齐、育苗困难等技术难题。[方法]以野蔷薇为试验材料,研究冷藏时间、激素处理、基质选择等因素对野蔷薇种子发芽的影响。[结果]结果表明,影响野蔷薇种子萌发的主要因素是冷藏时间;0.10和0.15 mg/L赤霉素(GA3)处理10 d,种子发芽率均达到70%以上;选用干净河沙作基质催芽效果优于其它材料。

Screening of Species-specific DNA Probes for Identification of Fallopia multiflora

To screen species-specific DNA probes for identification of Fallopia muhiflora, the genomic DNA （gDNA） suppression subtraction hybridization （SSH） between F. muhiflora and F. muhiflora var. ciliinervis was firstly performed. The obtained differential gDNA fragments by SSH were then hybridized with gDNA ar- rays consisting of multiple whole genomes of several species （adulterants and/or closely related species of F. muhiflora） and four differential fragments were screened uniquely representing F. muhiflora, which could be used as F. muhiflora species-specific probes. The screened DNA probes were tested by reverse dot blot hybridization and the results demonstrated that these probes could be used reliably to identify F, muhiflora. The species-specific DNA probes obtained in this study exhibited broad application prospects in the preparation of gene chips for identifying Chinese traditional medicines and the authentication of germplasm re- sources and crude drugs of F. muhiflora.

高效液相色谱法测定野蔷薇果中V_C含量的研究

建立一种高效液相色谱法测定野蔷薇中VC含量的新方法。采用Diamonsil5μ C18反相柱(200mm×4.6mm×5μm)分离;流动相为甲醇-0.01%磷酸水溶液(3:7,体积分数);柱温25℃;流速1.0mL/min;二级管陈列检测器,检测波长254nm。VC在浓度为20mg/L-1500mg/L范围内呈良好的线性关系,线性回归方程为y=17.136x+120.8,相关系数为0.9992,方法回收率为97%-101%相对标准偏差小于3%。该方法简便、快速、准确、适合于测定其它水果和蔬菜中VC的含量。

HPLC法测定甜地丁中芹菜素的含量

目的:建立甜地丁中芹菜素含量测定的方法。方法:色谱柱:ODS C_(18)(250 mm×4.6mm,5μm),流动相:甲醇-0.3%磷酸溶液(60:40),流速:1.0 ml·min^(-1),检测波长:339nm。结果:芹菜素在14.36～143.60 ng范围内呈良好的线性关系,r=0.999 8;平均回收率为98.7%,RSD=2.0%(n=6)。结论:本方法简便、结果准确、专属性强。