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NF-κB“decoy”寡核苷酸对LPS诱导巨噬细胞IL-10表达的影响 被引量:3
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作者 王建 邓小明 +1 位作者 李金宝 马胜忠 《中国病理生理杂志》 CAS CSCD 北大核心 2005年第3期565-568,共4页
目的 :探讨NF -κB“decoy”寡核苷酸 (ODNs)对脂多糖 (LPS)诱导小鼠巨噬细胞株J774 .1表达IL - 10的影响。方法 :通过体外细胞培养技术 ,观察转染NF -κB“decoy”ODNs对LPS刺激巨噬细胞产生IL - 10及其表达的影响。结果 :在LPS刺激的... 目的 :探讨NF -κB“decoy”寡核苷酸 (ODNs)对脂多糖 (LPS)诱导小鼠巨噬细胞株J774 .1表达IL - 10的影响。方法 :通过体外细胞培养技术 ,观察转染NF -κB“decoy”ODNs对LPS刺激巨噬细胞产生IL - 10及其表达的影响。结果 :在LPS刺激的巨噬细胞中转染NF -κB“decoy”ODNs对抗炎介质IL - 10的合成表达无影响。结论 :NF-κB“decoy”ODNs不抑制抗炎介质IL - 10的表达 ,可能由于NF -κB在IL - 10转录机制中不占主导地位。 展开更多
关键词 巨噬细胞 核因子NF-κB“decoy 寡核苷酸 白细胞介素-10
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Multi-features Based Approach for Moving Shadow Detection 被引量:4
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作者 周宁 周曼丽 +1 位作者 许毅平 方宝红 《Journal of Donghua University(English Edition)》 EI CAS 2004年第6期76-80,共5页
In the video-based surveillance application, moving shadows can affect the correct localization and detection of moving objects. This paper aims to present a method for shadow detection and suppression used for moving... In the video-based surveillance application, moving shadows can affect the correct localization and detection of moving objects. This paper aims to present a method for shadow detection and suppression used for moving visual object detection. The major novelty of the shadow suppression is the integration of several features including photometric invariant color feature, motion edge feature, and spatial feature etc. By modifying process for false shadow detected, the averaging detection rate of moving object reaches above 90% in the test of Hall-Monitor sequence. 展开更多
关键词 MOVING SHADOW detection multi - features MOVING OBJECT DETECTION
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The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) 被引量:40
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作者 Xiang-Qun Cui Yong-Heng Zhao +52 位作者 Yao-Quan Chu Guo-Ping Li Qi Li Li-Ping Zhang Hong-Jun Su Zheng-Qiu Yao Ya-Nan Wang Xiao-Zheng Xing Xin-Nan Li Yong-Tian Zhu Gang Wang Bo-Zhong Gu A-Li Luo Xin-Qi Xu Zhen-Chao Zhang Gen-Rong Liu Hao-Tong Zhang De-Hua Yang Shu-Yun Cao Hai-Yuan Chen Jian-Jun Chen Kun-Xin Chen Ying Chen Jia-Ru Chu Lei Feng Xue-Fei Gong Yong-Hui Hou Hong-Zhuan Hu Ning-Sheng Hu Zhong-Wen Hu Lei Jia Fang-Hua Jiang Xiang Jiang Zi-Bo Jiang Ge Jin Ai-Hua Li Yan Li Ye-Ping Li Guan-QunLiu Zhi-Gang Liu Huo-Ming Shi Zheng-Hong Tang Qing-Sheng Tao Xiang-Yan Yuan Chao Zhai Jing Zhang Yan-Xia Zhang Yong Zhang Ming Zhao Fang Zhou Guo-Hua Zhou Jie Zhu Si-Cheng Zou 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2012年第9期1197-1242,共46页
The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST, also called the Guo Shou Jing Telescope) is a special reflecting Schmidt telescope. LAMOST’s special design allows both a large aperture (effecti... The Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST, also called the Guo Shou Jing Telescope) is a special reflecting Schmidt telescope. LAMOST’s special design allows both a large aperture (effective aperture of 3.6 m–4.9 m) and a wide field of view (FOV) (5°). It has an innovative active reflecting Schmidt configuration which continuously changes the mirror’s surface that adjusts during the observation process and combines thin deformable mirror active optics with segmented active optics. Its primary mirror (6.67m×6.05 m) and active Schmidt mirror (5.74m×4.40 m) are both segmented, and composed of 37 and 24 hexagonal sub-mirrors respectively. By using a parallel controllable fiber positioning technique, the focal surface of 1.75 m in diameter can accommodate 4000 optical fibers. Also, LAMOST has 16 spectrographs with 32 CCD cameras. LAMOST will be the telescope with the highest rate of spectral acquisition. As a national large scientific project, the LAMOST project was formally proposed in 1996, and approved by the Chinese government in 1997. The construction started in 2001, was completed in 2008 and passed the official acceptance in June 2009. The LAMOST pilot survey was started in October 2011 and the spectroscopic survey will launch in September 2012. Up to now, LAMOST has released more than 480 000 spectra of objects. LAMOST will make an important contribution to the study of the large-scale structure of the Universe, structure and evolution of the Galaxy, and cross-identification of multiwaveband properties in celestial objects. 展开更多
关键词 techniques: wide field telescope - active optics - multi fiber - spectroscopy survey - data reduction
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A Comparative Study of Acute Hemodynamic Effects of Multi -site Cardiac Pacing in patients with cardiac function NYHA class ⅠtoⅡ without bundle branch block 被引量:2
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作者 朱参战 崔长琮 +5 位作者 张全发 薛小临 刘维维 刘引会 《South China Journal of Cardiology》 CAS 2001年第2期101-105,共5页
Objective To compare the acute hemodynamic effects of five different pacing modes in patients with cardiac function NYHA class Ⅰ to Ⅱ without bundle branch block (BBB). Methods This study included 12 patients (SSS ... Objective To compare the acute hemodynamic effects of five different pacing modes in patients with cardiac function NYHA class Ⅰ to Ⅱ without bundle branch block (BBB). Methods This study included 12 patients (SSS 7, Ⅲ°AVB 5) undergoing pacemaker implantation. Right ventricular apex (RVA), right ventricular outflow tract (RVOT), right ventricular bifocal (RV-Bi), left ventricular base (LVB) and bi -ventricular (Bi-Ⅴ) pacing at 60 -80 ppm were done in VVI mode prior to implantation of DDD pacemaker. The cardiac index (CI), mean pulmonary artery pressure (mPAP) and pulmonary capillary wedge pressure (PCWP) were measured with Swan - Ganz thermodilution catheter after 5 minutes of each pacing mode. Results (1) Comparing to pacing at RVA (CI: 2. 41± 0. 38 L/min per m2, PCWP: 16. 7 ±3.3 mmHg), the CI increased and the PCWP decreased significantly in pacing at RVOT(CI: 2. 63 ± 0.46, PCWP: 13. 8±2. 3), LVB(CI: 2. 78±0.52, PCWP: 14. 4±3.1), RV-Bi(CI: 2. 83±0.57, PCWP: 12. 8± 2. 5) and Bi -Ⅴ pacing (CI: 2. 94± 0.60, PCWP: 12. 7±2. 5), P < 0. 01, respectively. (2) The CI of RV-Bi and Bi-Ⅴ pacing was higher than that of RVOT and LVB pacing, the PCWP was lower, P < 0. 05, respectively. (3) There was no significant difference between RV - Bi pacing and Bi-Ⅴ pacing in CI and PCWP. Conclusion There is no significant difference between RV - Bi pacing and Bi -V pacing in the acute hemodynamic effects; however,dual - site pacing is much better than single site pacing in that aspect for patients with cardiac function NYHA class Ⅰ to Ⅱ without BBB. Among single site pacing, the RVOT and LVB pacing is better than RVA pacing in cardiac function. 展开更多
关键词 multi - site cardiac pacingHemodynamics
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Research on A Master - slave Multi - microcomputers Control System for Hollow Spindle Fancy Yarn Spinning Machine
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作者 李志蜂 陈子展 阵瑞琪 《Journal of China Textile University(English Edition)》 EI CAS 1999年第1期49-52,共4页
In this paper, a successfully studied and developed master - slave muld - microcomputers control system based on PC - BUS for hollow spindle fancy yarn spinning machine, mainly Its overall scheme, software and hardwar... In this paper, a successfully studied and developed master - slave muld - microcomputers control system based on PC - BUS for hollow spindle fancy yarn spinning machine, mainly Its overall scheme, software and hardware construction, is introduced. Spinning experiments show that the system achieves satisfactory result. This system can solve the diftkultles of mechatronical fusion between domestic hollow splndk fancy yarn spuming muchine and its microcomputer control technology. 展开更多
关键词 hollow SPINDLE FANCY YAM spinning machine mechatrvnical fusion MASTER - SLAVE multi - microcomputers control system PC - BUS.
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On the Representation of Multi-layer Woven Structure
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作者 易洪雷 丁辛 《Journal of China Textile University(English Edition)》 EI CAS 1999年第3期14-16,共3页
A quantitative method is developed to identify the structures of multi-layer woven fabric in this study. Six structural parameters are selected to represent the fabric structure. With some design criteria for the rein... A quantitative method is developed to identify the structures of multi-layer woven fabric in this study. Six structural parameters are selected to represent the fabric structure. With some design criteria for the reinforcement of textile composites, the relationships among the structural parameters are derived for different binding patterns of the fabric structure. The conditions, which meet the requirements of structural cell stability,are also discussed. 展开更多
关键词 multi - layer woven FABRIC structural parame-ter BINDING PATTERN TEXTILE composite .
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Computer-aided Pattern Design of Multi-bar Warp Knitted Fabrics
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作者 李炜 《Journal of China Textile University(English Edition)》 EI CAS 2000年第1期85-87,共3页
To meet the development trend of multi-bar warp knit-ting machine towards high-speed,advanced technologyand computer control and the requirements of variousproducts with small quantity,there are many researcheson the ... To meet the development trend of multi-bar warp knit-ting machine towards high-speed,advanced technologyand computer control and the requirements of variousproducts with small quantity,there are many researcheson the computer-aided pattern design of multi-barwarp knitted fabrics.In terms of the special propertiesof the computer-aided pattern design of multi-barwarp knitted fabrics,the Object Oriented Program(OOP)programming-Object Windows class Library(OWL)programming is selected.According to thecharacters of the OWL programming,various functionsare defined.Pattern design and technical parameters canbe output,which offers a great convenience for the fac-tory. 展开更多
关键词 multi - bar WARP KNITTED fabric computer -aided PATTERN design PATTERN Object Windows class Library (OWL) programming.
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MODS: A Novel Metaheuristic of Deterministic Swapping for the Multi-Objective Optimization of Combinatorials Problems
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作者 Elias David Nifio Ruiz Carlos Julio Ardila Hemandez +2 位作者 Daladier Jabba Molinares Agustin Barrios Sarmiento Yezid Donoso Meisel 《Computer Technology and Application》 2011年第4期280-292,共13页
This paper states a new metaheuristic based on Deterministic Finite Automata (DFA) for the multi - objective optimization of combinatorial problems. First, a new DFA named Multi - Objective Deterministic Finite Auto... This paper states a new metaheuristic based on Deterministic Finite Automata (DFA) for the multi - objective optimization of combinatorial problems. First, a new DFA named Multi - Objective Deterministic Finite Automata (MDFA) is defined. MDFA allows the representation of the feasible solutions space of combinatorial problems. Second, it is defined and implemented a metaheuritic based on MDFA theory. It is named Metaheuristic of Deterministic Swapping (MODS). MODS is a local search strategy that works using a MDFA. Due to this, MODS never take into account unfeasible solutions. Hence, it is not necessary to verify the problem constraints for a new solution found. Lastly, MODS is tested using well know instances of the Bi-Objective Traveling Salesman Problem (TSP) from TSPLIB. Its results were compared with eight Ant Colony inspired algorithms and two Genetic algorithms taken from the specialized literature. The comparison was made using metrics such as Spacing, Generational Distance, Inverse Generational Distance and No-Dominated Generation Vectors. In every case, the MODS results on the metrics were always better and in some of those cases, the superiority was 100%. 展开更多
关键词 METAHEURISTIC deterministic finite automata combinatorial problem multi - objective optimization metrics.
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NF-κB双链寡脱氧核苷酸圈套对严重胸外伤挫伤肺超微结构的影响 被引量:3
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作者 方丹青 于涛 万丽 《广东医学》 CAS CSCD 北大核心 2010年第13期1647-1649,共3页
目的研究NF-κB双链寡脱氧核苷酸圈套(NF-κB decoy ODN)对严重胸外伤肺挫伤血清NF-κB及肺组织超微结构的影响。方法 40只新西兰白兔随机分为:(1)A组,严重胸外伤肺挫伤(n=12);(2)B组,严重胸外伤肺挫伤NF-κB杂链decoy ODN治疗(n=12);(... 目的研究NF-κB双链寡脱氧核苷酸圈套(NF-κB decoy ODN)对严重胸外伤肺挫伤血清NF-κB及肺组织超微结构的影响。方法 40只新西兰白兔随机分为:(1)A组,严重胸外伤肺挫伤(n=12);(2)B组,严重胸外伤肺挫伤NF-κB杂链decoy ODN治疗(n=12);(3)C组,严重胸外伤肺挫伤NF-κB正链decoy ODN治疗(n=12);(4)D组,正常无损伤对照(n=4)。建立严重胸外伤肺损伤模型,按实验分组分别将合成的正链、杂链NF-κB decoy ODN经颈内静脉注入,每只实验兔分别注射20μg。于挫伤后1、2、3、4 h检测血清NF-κB及肺组织超微结构病理的改变。结果肺挫伤后血清中NF-κB含量1h升高,4 h达高峰。经NF-κB正链decoyODN治疗后2 h,升高的NF-κB开始下降,3 h达最低值。超微结构病理结果显示,D组肺组织肺泡结构正常,Ⅰ、Ⅱ型细胞清晰,或毛细血管轻微充血;A组挫伤后1 h时肺泡及毛细血管充血,2 h时可见肺泡腔内白细胞,毛细血管充血严重,有轻微出血;3 h后肺泡腔破损中等出血,毛细血管充血严重;而B组于挫伤后1 h肺泡腔少量出血,肺泡壁毛细血管充血,2 h时肺泡腔毛细血管出血,有红、白细胞释放至肺泡腔,3 h后肺泡腔出血严重,可见板层小体空化,染色质凝集;C组在肺挫伤后1 h肺泡腔无出血,肺泡壁毛细血管仅少量充血,2、3 h时毛细血管充血,肺泡腔可见出血,4 h后可见充血明显的肺泡壁毛细血管,肺泡腔内仅见少量红细胞。各组组间比较,肺挫伤后均有不同程度充血出血,但经正链decoy ODN治疗后,肺泡腔出血明显减少,说明正链NF-κB decoy ODN可使急性肺挫伤组织病变减轻。结论对严重胸外伤肺挫伤早期给予NF-κB正链decoy ODN治疗,可使血清NF-κB表达减少,并减轻挫伤肺组织超微病变,对急性肺挫伤发展具有一定阻断作用。 展开更多
关键词 核因子-κB decoy ODN 肺挫伤
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NF-κB靶向性哑铃形“圈套”ODNs抑制大脑皮质神经元氧糖剥夺/复氧后TNF-α的表达 被引量:3
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作者 何兰英 罗勇 《第三军医大学学报》 CAS CSCD 北大核心 2009年第4期318-321,共4页
目的设计合成靶向哑铃形寡核苷酸(oligodeoxynucleotides,ODNs)圈套,检测其对大鼠大脑皮质神经元氧糖剥夺/复氧(OGD/R)后TNF-α表达的抑制效应。方法原代大脑皮质神经元体外培养,建立氧糖剥夺/复氧模型,以正常组、OGD4h/R6h组、NF-κB d... 目的设计合成靶向哑铃形寡核苷酸(oligodeoxynucleotides,ODNs)圈套,检测其对大鼠大脑皮质神经元氧糖剥夺/复氧(OGD/R)后TNF-α表达的抑制效应。方法原代大脑皮质神经元体外培养,建立氧糖剥夺/复氧模型,以正常组、OGD4h/R6h组、NF-κB decoy ODNs组、无关decoy ODNs组、脂质体组为实验组,采用Western blot检测NF-κB p65蛋白表达,分别采用免疫细胞化学和逆转录-聚合酶链反应法(RT-PCR)检测TNF-α蛋白和mRNA表达。结果Westernblot检测显示NF-κB decoy ODNs组NF-κB P65蛋白表达明显低于OGD4h/R6h组、脂质体组、无关decoy ODNs组(P<0.05),与OGD4h/R6h组、脂质体组、无关decoy组相比NF-κB decoy ODNs组TNF-α蛋白和mRNA表达明显降低(P<0.05);而脂质体组、无关decoy ODNs组对NF-κB P65蛋白、TNF-α蛋白和mRNA表达未见抑制(P>0.05)。结论靶向性NF-κB的哑铃形圈套ODNs可有效抑制大脑皮质神经元OGD/R后炎症因子TNF-αmRNA及蛋白的表达。 展开更多
关键词 NF-ΚB decoy ODNs 大脑皮质神经元 氧糖剥夺/复氧 肿瘤坏死因子-Α
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Pressure Gradient Force,Saffman Lift,and Magnus Lift on the Fiber-like Particle in Fluid 被引量:5
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作者 朱泽飞 林建忠 《Journal of China Textile University(English Edition)》 EI CAS 2000年第2期23-27,共5页
Flher-like particle suspensions are common in both na-ture and industry, but there is little work reported on it.The forces acting on the fiber - like particle in fluid arestudied in this paper, and the Magnus lift, S... Flher-like particle suspensions are common in both na-ture and industry, but there is little work reported on it.The forces acting on the fiber - like particle in fluid arestudied in this paper, and the Magnus lift, Saffman lift,pressure gradient force, and then the dynamics modelhave been received. The numerical study of the simpleshear flow past the cylinders shows that the particles ’motion is controlled by the vortex. 展开更多
关键词 multi - phase FLUID FIBER - LIKE PARTICLE FORCE analysis
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总氮含量分析方法比较及黑龙江典型淡水养殖池塘和自然水域总氮含量的变化 被引量:4
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作者 黄丽 覃东立 +4 位作者 吴松 陈中祥 高磊 黄晓丽 王鹏 《水产学杂志》 CAS 北大核心 2018年第4期37-42,共6页
应用Multi N/C2100S分析仪与紫外-可见分光光度计对1个有证标准样品和6个实际水样总氮含量进行对比实验,予以验证。结果表明:两种仪器对已知浓度的有证标准样品测得的平均值接近真值,RSD<1%、相对误差<5%,结果准确度和精密度无显... 应用Multi N/C2100S分析仪与紫外-可见分光光度计对1个有证标准样品和6个实际水样总氮含量进行对比实验,予以验证。结果表明:两种仪器对已知浓度的有证标准样品测得的平均值接近真值,RSD<1%、相对误差<5%,结果准确度和精密度无显著差异。Multi N/C2100S分析仪可以高效、快速批量测定。5个月中4次监测6个实际水样总氮含量的结果为:白鱼泡水体中TN含量无显著变化;宾县1~#和3~#池塘水体含量逐月升高;5月稻田TN含量明显偏高,可能是水稻生长施肥所致;7月松花江水样TN含量高于其他月份,可能受养殖密度、模式、天气、投饵量、施肥量及周边农作物施药量的影响。 展开更多
关键词 总氮(TN) 紫外-可见分光光度计 multi N/C2100S分析仪 淡水养殖池塘 自然水域
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CRE-decoy ODN对慢性吗啡作用的SK-N-SH细胞中相关信号分子的影响
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作者 段立华 张瑾 《中国新药杂志》 CAS CSCD 北大核心 2009年第11期1032-1037,共6页
目的:以环磷酸腺苷(cAMP)反应元件结合蛋白(Cyclic-AMP response-element-binding protein,CREB)为靶点,体外合成CRE转录因子诱骗寡核苷酸(CRE-transcription factor decoy oligodeoxynucleotide,CRE-decoy ODN),观察其对慢性吗啡作用... 目的:以环磷酸腺苷(cAMP)反应元件结合蛋白(Cyclic-AMP response-element-binding protein,CREB)为靶点,体外合成CRE转录因子诱骗寡核苷酸(CRE-transcription factor decoy oligodeoxynucleotide,CRE-decoy ODN),观察其对慢性吗啡作用和纳络酮催促戒断的人神经母细胞瘤细胞株(SK-N-SH)细胞cAMP含量、磷酸化CREB-1及CREB-1表达的影响,为在分子水平上寻找阿片类依赖的干预靶点提供实验依据。方法:体外合成CRE-decoy ODN,以DOTAP(1,2-dioleoyl-3-trimethylammonium propane)为转移介质,将CRE-decoy ODN与慢性吗啡作用和纳络酮催促戒断的SK-N-SH细胞共同孵育,电泳迁移率改变分析(EMSA)检测CRE-decoy ODN与转录因子CREB结合的序列特异性;采用非变性聚丙烯酰胺凝胶电泳及放射自显影检测CRE-decoy ODN细胞摄取;放射免疫分析法(RIA)检测细胞cAMP含量;Western blot检测CREB-1及磷酸化CREB-1表达。结果:单纯吗啡组较生理氯化钠溶液对照组细胞cAMP水平显著升高(P<0.05),单纯CRE-decoy ODN组与生理氯化钠溶液对照组无明显差异(P>0.05),但能明显抑制吗啡及纳络酮组的cAMP水平(P<0.05);单纯吗啡组与生理氯化钠溶液对照组比较,磷酸化CREB-1表达明显增高(P<0.01),吗啡+纳络酮组较单纯吗啡组轻度降低,与对照组比较仍有显著差异(P<0.01);CRE-decoyODN可抑制单纯吗啡组及吗啡+纳络酮组磷酸化CREB-1表达明显增高(P均<0.05)。结论:CRE-decoyODN可抑制慢性吗啡作用及纳络酮催促戒断引起SK-N-SH细胞cAMP和磷酸化CREB表达的升高。 展开更多
关键词 吗啡 SK—N—SH细胞株 CRE转录因子诱骗寡核苷酸(CRE—decoy ODN)CRE—decoy ODN 磷酸腺苷(cAMP) cAMP反应元件结合蛋白-1(CREB-1) 磷酸化CREB-1
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Some Practical Issues Regarding Java Programming
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作者 Ming-fang Wang Department of Computer Science, University of Central ArkansasConway, Arkansas 72035 wangm @ panda.cs.uca.eduWu JieShanghai Institute of Computing Tech. Shanghai, China 200040 Telephone: 62520070 《计算机工程》 CAS CSCD 北大核心 1996年第S1期106-121,共16页
Java programming language is a major . new development which involves object-oriented programming and computer networking. Here, several examples of Java programs are presented. These examples show some major features... Java programming language is a major . new development which involves object-oriented programming and computer networking. Here, several examples of Java programs are presented. These examples show some major features that Java language provides, such as multi-thread, exception handling, network application writing, and the usage of Java applets. This article also shows some ways of setting up a Java language programming environment. All these examples are correct and ready to run. 展开更多
关键词 Java programming/object- ORIENTED programming/multi - THREAD
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ANALYSIS OF PSYCHOLOGIC HEALTH STATE AND INFLUENCING FACTORS IN COLLEGE AND SECONDARY SCHOOL STUDENTS IN SHAANXI PROVINCE
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作者 高成阁 王友平 +1 位作者 谭新 亢万虎 《Academic Journal of Xi'an Jiaotong University》 2000年第2期168-171,共5页
Objective The psychologic health level of college and secondaryschool students and the relevant factors were investigated to scientific basis and guidance for school mental health work.Methods Standard 1251 cases were... Objective The psychologic health level of college and secondaryschool students and the relevant factors were investigated to scientific basis and guidance for school mental health work.Methods Standard 1251 cases were drawn from 1‰ of students in colleges and middle schools of Shaanxi province.Taking 14 psychic health level indexes in SCL 90 as dependent variable and 109 indexes of psychic health back ground as in dependent variable, multi factor analyses have been made.Results 22.6% of students had relatively serious psychological problems.The score of SCL 90 in females was a little bit higher than that in males.The scores of students at both universities and senior middle schools were higher than that in junior middle schools students.The score of SCL 90 of students who came from the countryside was higher than that of city students.The score of the whole students was higher than that of the normal.The students with psychic problems showed obsession,interpersonal sensitivity,depression,anxiety,paranoia and hostility.Factor analysis showed that influencing factors included history of positive individual risking behavior,physical conditions,grade,address,family influences,menses and sexual prombles,bad relation with others,poor self assessment.Conclusion The psychologic health level of the students investigated is lower than that of the whole society.The factors,which hamper psychic health of students, are biological,psychological and social in nature. 展开更多
关键词 school psychologic health SCL -90 multi factor analysis influencing factors
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核因子κB诱捕抑制白细胞介素-1β诱导的软骨细胞生成一氧化氮的实验研究 被引量:3
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作者 刘建湘 杜靖远 +3 位作者 杨述华 刘日光 易诚青 李新春 《中华风湿病学杂志》 CAS CSCD 2005年第6期346-348,i003,共4页
目的探讨用核因子诱捕(decoy)方法抑制软骨细胞生成一氧化氮(NO)的可行性。方法培养大鼠软骨细胞,人工合成大鼠诱生型一氧化氮合酶(iNOS)基因上能够与核因子(NF)-κB序列识别和结合并带有荧光标记的特异性双链寡脱氧核苷酸(ODN)序列NF-... 目的探讨用核因子诱捕(decoy)方法抑制软骨细胞生成一氧化氮(NO)的可行性。方法培养大鼠软骨细胞,人工合成大鼠诱生型一氧化氮合酶(iNOS)基因上能够与核因子(NF)-κB序列识别和结合并带有荧光标记的特异性双链寡脱氧核苷酸(ODN)序列NF-κBdecoyODN,将不同浓度的NF-κBdecoyODN转染入培养的大鼠软骨细胞,通过荧光显微镜观察其转染效率及降解情况,检测其对白细胞介素(IL)-1β诱导的软骨细胞NO生成及iNOSmRNA转录的影响。结果NF-κBdecoyODN能转染入软骨细胞胞核并能维持一定时间,其中4μmol/L以上浓度的NF-κBdecoyODN能够有效抑制IL-1β诱导的软骨细胞iNOSmRNA的转录及NO生成。结论NF-κBdecoyODN能够转染入软骨细胞并抑制IL-1β诱导的软骨细胞iNOSmRNA的转录及NO生成,为抑制NO的生成提供了新的思路和方法。 展开更多
关键词 白细胞介素- 细胞生成 核因子ΚB 实验研究 诱捕 诱生型一氧化氮合酶 decoy 大鼠软骨细胞 寡脱氧核苷酸 iNOS NO生成 mRNA转录 ODN mol/L 荧光标记 人工合成 不同浓度 转染效率 微镜观察 抑制NO NF 特异性 培养 序列
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