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茉莉花PAL基因家族的多基因组鉴定与表达分析
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作者 张宇航 谷梦雅 +3 位作者 洪雅萍 林宏政 金珊 叶乃兴 《江苏农业学报》 CSCD 北大核心 2024年第3期403-414,共12页
为深入探究茉莉花PAL基因家族的功能及在香气成分形成中的作用,本研究以福州单瓣茉莉花、双瓣茉莉花和重瓣茉莉花基因组数据为基础,利用生物信息学方法及实时荧光定量PCR技术,对JsPAL基因家族进行多基因组分析。结果表明,在单瓣茉莉花... 为深入探究茉莉花PAL基因家族的功能及在香气成分形成中的作用,本研究以福州单瓣茉莉花、双瓣茉莉花和重瓣茉莉花基因组数据为基础,利用生物信息学方法及实时荧光定量PCR技术,对JsPAL基因家族进行多基因组分析。结果表明,在单瓣茉莉花、双瓣茉莉花和重瓣茉莉花全基因组中分别鉴定出4个、1个和4个PAL家族基因,JsPAL家族成员均具有丙氨酸-丝氨酸-甘氨酸(Ala-Ser-Gly)组成的三肽活性中心。共线性分析发现单瓣茉莉花、重瓣茉莉花和拟南芥的PAL基因间的共线性关系与双瓣茉莉花相比更强;系统发育树分析结果显示,JsPAL基因家族分布在2个亚家族中。JsPAL基因启动子区域存在大量与胁迫响应、激素响应、光响应和植物生长相关的顺式调控元件。荧光定量分析结果表明,JsPAL在茉莉花组织中存在特异性表达,多数JsPAL在花中的相对表达量高于其他组织;大多数JsPAL基因在福州单瓣茉莉花中的相对表达量高于双瓣茉莉花;福州单瓣茉莉花中SP_JsPAL2、SP_JsPAL4、MP_JsPAL1和MP_JsPAL3在其花朵预开放期(F3)的相对表达量显著高于其他时期,双瓣茉莉花中DP_JsPAL1在F3阶段的相对表达量也较高;SP_JsPAL1和SP_JsPAL2与2-苯乙醇和苯乙醛含量呈显著正相关,其中SP_JsPAL1还与苯甲醛含量呈显著正相关。说明,JsPAL可能在茉莉花的发育中起重要作用,SP_JsPAL2、SP_JsPAL4、MP_JsPAL1、MP_JsPAL3和DP_JsPAL1可能对福州单瓣茉莉花、双瓣茉莉花的香气成分形成具有重要的调控作用,SP_JsPAL1可能对福州单瓣茉莉鲜灵香气的形成具有重要作用。 展开更多
关键词 茉莉花 苯丙氨酸解氨酶(PAL) 表达分析 多基因组
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Energy-based numerical models for assessment of soil liquefaction 被引量:7
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作者 Amir Hossein Alavi Amir Hossein Gandomi 《Geoscience Frontiers》 SCIE CAS 2012年第4期541-555,共15页
This study presents promising variants of genetic programming (GP), namely linear genetic programming (LGP) and multi expression programming (MEP) to evaluate the liquefaction resistance of san- dy soils. Genera... This study presents promising variants of genetic programming (GP), namely linear genetic programming (LGP) and multi expression programming (MEP) to evaluate the liquefaction resistance of san- dy soils. Generalized LGP and MEP-based relationships were developed between the strain energy density required to trigger liquefaction (capacity energy) and the factors affecting the liquefaction characteristics of sands. The correlations were established based on well established and widely dispersed experimental results obtained from the literature. To verify the applicability of the derived models, they were employed to estimate the capacity energy values of parts of the test results that were not included in the analysis. The external validation of the models was verified using statistical criteria recommended by researchers. Sensitivity and parametric analyses were performed for further verification of the correlations. The results indicate that the proposed correlations are effectively capable of capturing the liquefaction resistance of a number of sandy soils. The developed correlations provide a significantly better prediction performance than the models found in the literature. Furthermore, the best LGP and MEP models perform superior than the optimal traditional GP model. The verification phases confirm the efficiency of the derived correlations for their general application to the assessment of the strain energy at the onset of liquefaction. 展开更多
关键词 Soil liquefaction Capacity energy Linear genetic programming multi expression programming SAND FORMULATION
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