In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the majo...In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source,to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds.Screening using this medium identified potent denitrifying Bacillus isolates,among which Bacillus subtilis M7-1 was considered best.M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen.Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH(5.0–9.0),salinity(0–30)and temperature(25–35℃).The denitrifying enzyme gene was amplified,sequenced and further identified as nirS type.In biosecurity assessments,M7-1 had no negative effects on shrimps at a dose of 106 cfu mL−1.M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration,and to promote the development of sustainable and healthy culture systems.展开更多
This study was conducted to evaluate the alleviation of Bacillus subtilis ANSB01G culture as zearalenone(ZEA)biodegradation agent on oxidative stress,cell apoptosis and fecal ZEA residue in the first parity gestation ...This study was conducted to evaluate the alleviation of Bacillus subtilis ANSB01G culture as zearalenone(ZEA)biodegradation agent on oxidative stress,cell apoptosis and fecal ZEA residue in the first parity gestation sows during the gestation.A total of 80 first-parity gilts(Yorkshire×Landrace)were randomly allocated to 4 dietary treatments with 20 replications per treatment and one gilt per replicate.The dietary treatments were as follows:CO(positive control);MO(negative control,ZEA level at 246μg/kg diet);COA(CO+B.subtilis ANSB01G culture with 2×10^9 CFU/kg diet);MOA(MO+ZEA level at 260μg/kg diet+B.subtilis ANSB01G culture with 2×10^9 CFU/kg diet).The experiment lasted for the whole gestation period of sows.Results showed that feeding the diet naturally contaminated with low-dose ZEA caused an increase of cell apoptosis in organ and the residual ZEA in feces as well as a decrease of antioxidant function in serum.The addition of B.subtilis ANSB01G culture in the diets can effectively alleviate the status of oxidative stress and cell apoptosis induced by ZEA in diets of gestation sows,as well as decrease the content of residual ZEA in feces.展开更多
基金supported by the Special Fund for Qingdao Marine Biomedical science and Technology Innovation Center, China (No. 2017-CXZX01-3-13)
文摘In this study,a novel culture medium that simulates shrimp pond conditions was established to screen nitrite-degrading isolates.The medium was supplemented with nitrite as a nitrogen source and shrimp feed as the major carbon source,to achieve the high nitrogen and low carbon nutritional status found in shrimp farming ponds.Screening using this medium identified potent denitrifying Bacillus isolates,among which Bacillus subtilis M7-1 was considered best.M7-1 was able to completely degrade nitrite-N in 24 h without much consumption of dissolved oxygen.Efficient denitrification activity took place in liquid cultures within a set of non-stringent ranges of pH(5.0–9.0),salinity(0–30)and temperature(25–35℃).The denitrifying enzyme gene was amplified,sequenced and further identified as nirS type.In biosecurity assessments,M7-1 had no negative effects on shrimps at a dose of 106 cfu mL−1.M7-1 could therefore be used in aquaculture to reduce and control the nitrogen concentration,and to promote the development of sustainable and healthy culture systems.
基金supported by National Natural Science Foundation of China(Grant No.31572447)Beijing Municipal Natural Science Foundation(Grant No.6172017)a Special Fund for Agroscientific Research in the Public Interest(201403047)
文摘This study was conducted to evaluate the alleviation of Bacillus subtilis ANSB01G culture as zearalenone(ZEA)biodegradation agent on oxidative stress,cell apoptosis and fecal ZEA residue in the first parity gestation sows during the gestation.A total of 80 first-parity gilts(Yorkshire×Landrace)were randomly allocated to 4 dietary treatments with 20 replications per treatment and one gilt per replicate.The dietary treatments were as follows:CO(positive control);MO(negative control,ZEA level at 246μg/kg diet);COA(CO+B.subtilis ANSB01G culture with 2×10^9 CFU/kg diet);MOA(MO+ZEA level at 260μg/kg diet+B.subtilis ANSB01G culture with 2×10^9 CFU/kg diet).The experiment lasted for the whole gestation period of sows.Results showed that feeding the diet naturally contaminated with low-dose ZEA caused an increase of cell apoptosis in organ and the residual ZEA in feces as well as a decrease of antioxidant function in serum.The addition of B.subtilis ANSB01G culture in the diets can effectively alleviate the status of oxidative stress and cell apoptosis induced by ZEA in diets of gestation sows,as well as decrease the content of residual ZEA in feces.
