We investigate the propagation of polarized light in fibrous tissues such as muscle and skin.The myofibrils and collagen fibers are approximated as long cylinders and the tissue phantom is composed of spherical and cy...We investigate the propagation of polarized light in fibrous tissues such as muscle and skin.The myofibrils and collagen fibers are approximated as long cylinders and the tissue phantom is composed of spherical and cylindrical structures.We apply Monte Carlo method based on this phantom to simulate and analyze polarization imaging process of muscle.The good agreement between the simulation results and the experimental results validate the assumption of the phantom composition.This paper also presents how to describe the fiber orientation distribution and tissue anisotropy according to three parameters derived from the polarization imaging.展开更多
The performance of nanoparticles is often affected by particle size and morphology.Currently,electron microscopy or atomic force microscopy is typically utilized to determine the size and morphology of nanoparticles.H...The performance of nanoparticles is often affected by particle size and morphology.Currently,electron microscopy or atomic force microscopy is typically utilized to determine the size and morphology of nanoparticles.However,there are issues such as difficult sample preparation,long processing times,and challenges in quantitative characterization.Therefore,it is of great significance to develop a fast,accu-rate,and statistical method to measure the size and morphology of nanoparticles.In this study,a new method,called polarized imaging dynamic light scattering(PIDLS),is proposed.The nanoparticles are irradiated with a vertical linearly polarized laser beam,and a polarization camera collected the dynamic light scattering images of particles at four different polarization directions(0°,45°,90°,and 135°)at a scattering angle of 90°.The average particle size and distribution are obtained using the imaging dy-namic light scattering method at 0°polarization direction,and the morphology of the particles is ob-tained based on the depolarization patterns of the scattered light.The optical sphericityΦis defined based on the degree of linear polarization(DoLP).It is also implemented for the quantitative evaluation of the sphericity of the nanoparticles,including spherical,octahedral,nanoplate,nanorod,and linear ones.Together with the Poincarésphere parameterψ,the morphology of the nanoparticles can be roughly identified.In addition,PIDLS enables the measurement of particle size and morphology distributions simultaneously for evaluating the uniformity of particles.The effectiveness of PIDLS is verified by the measurement of five kinds of industrial titanium dioxide as well.展开更多
A homemade Static Light scattering studies has been used to determine angle resolved scattered intensity for different polarization states of the incident laser light. Classical light scattering set ups are being used...A homemade Static Light scattering studies has been used to determine angle resolved scattered intensity for different polarization states of the incident laser light. Classical light scattering set ups are being used to study morphological aspects of scatterers using simple set ups using low power lasers. Red blood cells form rather interesting as well as a challenging system for scattering experiments. The scattering spectrometer consists of a scattering arm, a scattering turn table and collimating arm. Along with polarizers integrated in the collimating arm as well as scattering arms ensures collection of scattered flux with the required polarization state. This technique is being developed for its in vitro studies using fresh red blood cells. A brief review of the theoretical models used for scattering from Red Blood Cells (RBC) has been discussed in the paper. Scattering pattern (scattering plots) as well as polar plots of scattered flux have been determined for different polarization state of the incident light. Insight into the orientation of major axis of particles can be inferred from the polar plots.展开更多
The quantitative optical measurement of deep sub-wavelength features with sub-nanometer sensitivity addresses the measurement challenge in the semiconductor fabrication process.Optical scatterings from the sidewalls o...The quantitative optical measurement of deep sub-wavelength features with sub-nanometer sensitivity addresses the measurement challenge in the semiconductor fabrication process.Optical scatterings from the sidewalls of patterned devices reveal abundant structural and material information.We demonstrated a parametric indirect microscopic imaging(PIMI)technique that enables recovery of the profile of wavelength-scale objects with deep sub-wavelength resolution,based on measuring and filtering the variations of far-field scattering intensities when the illumination was modulated.The finite-difference time-domain(FDTD)numerical simulation was performed,and the experimental results were compared with atomic force microscopic(AFM)images to verify the resolution improvement achieved with PIMI.This work may provide a new approach to exploring the detailed structure and material properties of sidewalls and edges in semiconductor-patterned devices with enhanced contrast and resolution,compared with using the conventional optical microscopy,while retaining its advantage of a wide field of view and relatively low cost.展开更多
In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment ...In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobulin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were con- sistent with the data measured by dynamic light scattering (DLS) technique.展开更多
The optical wave scattering from the slightly rough surface of three-layer medium is studied. The formulaes of the scattering coefficients for different polarizations are derived using the small perturbation method. A...The optical wave scattering from the slightly rough surface of three-layer medium is studied. The formulaes of the scattering coefficients for different polarizations are derived using the small perturbation method. A Gaussian rough surface is presented for describing rough surface of layered medium, the influence of the permittivity of layered medium, the mean layer thickness of intermediate medium, the surface roughness parameters and the incident wavelength on the bistatic scattering coefficient of HH polarization are obtained and discussed by numerical implementation.展开更多
本文叙述了新建立的磁场效应(Magnetic Field Effects,简称MFE_s)-偏振-共振同步荧光法。利用偏振技术抑制掉溶剂散射光的99％,利用MFE_s抑制溶剂98％的散射光波动。将偏振、MFE_s技术与共振同步荧光法结合为一体时,则大大改善Δλ=0的...本文叙述了新建立的磁场效应(Magnetic Field Effects,简称MFE_s)-偏振-共振同步荧光法。利用偏振技术抑制掉溶剂散射光的99％,利用MFE_s抑制溶剂98％的散射光波动。将偏振、MFE_s技术与共振同步荧光法结合为一体时,则大大改善Δλ=0的共振同步荧光法的检测灵敏度。如蒽、苝舵同时测定时,其检测限分别为0.068ng/ml、0.015ng/ml。优于一般的Δλ值较小(0～5nm)的同步荧光法和偏振-同步荧光法。展开更多
基金National Natural Science Foundation of China(Grant 60578003)Ministry of Science and Technology(Grant 2006CB70570),China.
文摘We investigate the propagation of polarized light in fibrous tissues such as muscle and skin.The myofibrils and collagen fibers are approximated as long cylinders and the tissue phantom is composed of spherical and cylindrical structures.We apply Monte Carlo method based on this phantom to simulate and analyze polarization imaging process of muscle.The good agreement between the simulation results and the experimental results validate the assumption of the phantom composition.This paper also presents how to describe the fiber orientation distribution and tissue anisotropy according to three parameters derived from the polarization imaging.
基金supported by Shanghai Sailing Program(grant No.22YF1429600).
文摘The performance of nanoparticles is often affected by particle size and morphology.Currently,electron microscopy or atomic force microscopy is typically utilized to determine the size and morphology of nanoparticles.However,there are issues such as difficult sample preparation,long processing times,and challenges in quantitative characterization.Therefore,it is of great significance to develop a fast,accu-rate,and statistical method to measure the size and morphology of nanoparticles.In this study,a new method,called polarized imaging dynamic light scattering(PIDLS),is proposed.The nanoparticles are irradiated with a vertical linearly polarized laser beam,and a polarization camera collected the dynamic light scattering images of particles at four different polarization directions(0°,45°,90°,and 135°)at a scattering angle of 90°.The average particle size and distribution are obtained using the imaging dy-namic light scattering method at 0°polarization direction,and the morphology of the particles is ob-tained based on the depolarization patterns of the scattered light.The optical sphericityΦis defined based on the degree of linear polarization(DoLP).It is also implemented for the quantitative evaluation of the sphericity of the nanoparticles,including spherical,octahedral,nanoplate,nanorod,and linear ones.Together with the Poincarésphere parameterψ,the morphology of the nanoparticles can be roughly identified.In addition,PIDLS enables the measurement of particle size and morphology distributions simultaneously for evaluating the uniformity of particles.The effectiveness of PIDLS is verified by the measurement of five kinds of industrial titanium dioxide as well.
文摘A homemade Static Light scattering studies has been used to determine angle resolved scattered intensity for different polarization states of the incident laser light. Classical light scattering set ups are being used to study morphological aspects of scatterers using simple set ups using low power lasers. Red blood cells form rather interesting as well as a challenging system for scattering experiments. The scattering spectrometer consists of a scattering arm, a scattering turn table and collimating arm. Along with polarizers integrated in the collimating arm as well as scattering arms ensures collection of scattered flux with the required polarization state. This technique is being developed for its in vitro studies using fresh red blood cells. A brief review of the theoretical models used for scattering from Red Blood Cells (RBC) has been discussed in the paper. Scattering pattern (scattering plots) as well as polar plots of scattered flux have been determined for different polarization state of the incident light. Insight into the orientation of major axis of particles can be inferred from the polar plots.
基金the National Major Scientific Instruments and Equipment Development Project under Grant No.61827814the National Natural Science Foundation of China(NSFC)under Grant No.61501239+5 种基金the NSFC International Young Scientist Research Fund under Grant No.61750110520the Hubei Polytechnic University Laboratory Fund under Grant No.19XJK24Rthe Jiangsu Postdoc Research Fund under Grant No.1601001Bthe Beijing Natural Science Foundation under Grant No.Z190018the Fundamental Research Funds for the Central Universities under Grant No.30920010011the UK Engineering and Physical Sciences Research Council under Grant No.EP/R042578/1。
文摘The quantitative optical measurement of deep sub-wavelength features with sub-nanometer sensitivity addresses the measurement challenge in the semiconductor fabrication process.Optical scatterings from the sidewalls of patterned devices reveal abundant structural and material information.We demonstrated a parametric indirect microscopic imaging(PIMI)technique that enables recovery of the profile of wavelength-scale objects with deep sub-wavelength resolution,based on measuring and filtering the variations of far-field scattering intensities when the illumination was modulated.The finite-difference time-domain(FDTD)numerical simulation was performed,and the experimental results were compared with atomic force microscopic(AFM)images to verify the resolution improvement achieved with PIMI.This work may provide a new approach to exploring the detailed structure and material properties of sidewalls and edges in semiconductor-patterned devices with enhanced contrast and resolution,compared with using the conventional optical microscopy,while retaining its advantage of a wide field of view and relatively low cost.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 30570465 and 20425517)
文摘In acid buffer solution, proteins with positive charge can react with anion surfactant and result in a great enhancement of synchronous light scattering (SLS) signals. In this contribution, the correlative experiment was made to compare the interaction of human serum albumin (HSA) and immunoglobulin G (IgG) with sodium dodecyl sulfonate (SDS). Based on the measurements of the polarization light scattering signals, a new method of scattering polarization was constituted to distinguish these two interaction systems with molecular weight difference (HSA 66 kDa; IgG 150 kDa). The results were con- sistent with the data measured by dynamic light scattering (DLS) technique.
基金This work was supported by the National Natural Science Foundation of China (No.60571058) the National Defense Foundation of China.
文摘The optical wave scattering from the slightly rough surface of three-layer medium is studied. The formulaes of the scattering coefficients for different polarizations are derived using the small perturbation method. A Gaussian rough surface is presented for describing rough surface of layered medium, the influence of the permittivity of layered medium, the mean layer thickness of intermediate medium, the surface roughness parameters and the incident wavelength on the bistatic scattering coefficient of HH polarization are obtained and discussed by numerical implementation.
文摘本文叙述了新建立的磁场效应(Magnetic Field Effects,简称MFE_s)-偏振-共振同步荧光法。利用偏振技术抑制掉溶剂散射光的99％,利用MFE_s抑制溶剂98％的散射光波动。将偏振、MFE_s技术与共振同步荧光法结合为一体时,则大大改善Δλ=0的共振同步荧光法的检测灵敏度。如蒽、苝舵同时测定时,其检测限分别为0.068ng/ml、0.015ng/ml。优于一般的Δλ值较小(0～5nm)的同步荧光法和偏振-同步荧光法。
