In order to avoid the influence of noise variance on the filtering performances, a modified adaptive weighted averaging (MAWA) filtering algorithm is proposed for noisy image sequences. Based upon adaptive weighted av...In order to avoid the influence of noise variance on the filtering performances, a modified adaptive weighted averaging (MAWA) filtering algorithm is proposed for noisy image sequences. Based upon adaptive weighted averaging pixel values in consecutive frames, this algorithm achieves the filtering goal by assigning smaller weights to the pixels with inappropriate estimated motion trajectory for noise. It only utilizes the intensity of pixels to suppress noise and accordingly is independent of noise variance. To evaluate the performance of the proposed filtering algorithm, its mean square error and percentage of preserved edge points were compared with those of traditional adaptive weighted averaging and non-adaptive mean filtering algorithms under different noise variances. Relevant results show that the MAWA filtering algorithm can preserve image structures and edges under motion after attenuating noise, and thus may be used in image sequence filtering.展开更多
Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful ...Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful compared to the original input images,image fusion merges two or more initial images of the same item.Image fusion aims to produce,enhance,and transform significant elements of the source images into combined images for the sake of human visual perception.Image fusion is commonly employed for feature extraction in smart robots,clinical imaging,audiovisual camera integration,manufacturing process monitoring,electronic circuit design,advanced device diagnostics,and intelligent assembly line robots,with image quality varying depending on application.The research paper presents various methods for merging images in spatial and frequency domains,including a blend of stable and curvelet transformations,everageMax-Min,weighted principal component analysis(PCA),HIS(Hue,Intensity,Saturation),wavelet transform,discrete cosine transform(DCT),dual-tree Complex Wavelet Transform(CWT),and multiple wavelet transform.Image fusion methods integrate data from several source images of an identical target,thereby enhancing information in an extremely efficient manner.More precisely,in imaging techniques,the depth of field constraint precludes images from focusing on every object,leading to the exclusion of certain characteristics.To tackle thess challanges,a very efficient multi-focus wavelet decomposition and recompositionmethod is proposed.The use of these wavelet decomposition and recomposition techniques enables this method to make use of existing optimized wavelet code and filter choice.The simulated outcomes provide evidence that the suggested approach initially extracts particular characteristics from images in order to accurately reflect the level of clarity portrayed in the original images.This study enhances the performance of the eXtreme Gradient Boosting(XGBoost)algorithm in detecting brain malignancies with greater precision through the integration of computational image analysis and feature selection.The performance of images is improved by segmenting them employing the K-Means algorithm.The segmentation method aids in identifying specific regions of interest,using Particle Swarm Optimization(PCA)for trait selection and XGBoost for data classification.Extensive trials confirm the model’s exceptional visual performance,achieving an accuracy of up to 97.067%and providing good objective indicators.展开更多
There is a transition region between objects and background in any gray image. Many valuable applications of image segmentation and edge detection based on transition region determination have been developed in recent...There is a transition region between objects and background in any gray image. Many valuable applications of image segmentation and edge detection based on transition region determination have been developed in recent years. But, the complexity of calculation for determining transition region is too high. It results in the very limitation of applications based on transition region. A new novel fast method for transition region determination is presented in this paper, which will reduce the complexity of calculation dramatically. Many experiments have showed that this algorithm is effective and correct and will lay a good foundation for applications based on transition region. Key words image segmentation - transition region - maximum point - efficient average gradient (EAG) CLC number TP 391.4 Biography: Zhang Ai-hua (1965-), male, Ph. D candidate, research direction: image processing.展开更多
In this paper, a test system was developed in which a CCD camera was used as a sensor together with an IBM-AT-compatible computer with an Intel 80486 processor to measure the impurities and neps on a piece of cotton g...In this paper, a test system was developed in which a CCD camera was used as a sensor together with an IBM-AT-compatible computer with an Intel 80486 processor to measure the impurities and neps on a piece of cotton gray goods, and a method was proposed by which the differences of degree of gray between samples and interference caused by surface unevenness, creasing and the like within a test sample can be removed effectively. The whole test system is reliable, accurate and causing fewer subjective errors.展开更多
As one of the important sea state parameters for navigation safety and coastal resource management, the ocean wave direction represents the propagation direction of the wave. A novel algorithm based on an optical flow...As one of the important sea state parameters for navigation safety and coastal resource management, the ocean wave direction represents the propagation direction of the wave. A novel algorithm based on an optical flow method is developed for the ocean wave direction inversion of the ocean wave fields imaged by the X-band radar continuously. The proposed algorithm utilizes the echo images received by the X-band wave monitoring radar to estimate the optical flow motion, and then the actual wave propagation direction can be obtained by taking a weighted average of the motion vector for each pixel. Compared with the traditional ocean wave direction inversion method based on frequency-domain, the novel algorithm is fully using a time-domain signal processing method without determination of a current velocity and a modulation transfer function(MTF). In the meantime,the novel algorithm is simple, efficient and there is no need to do something more complicated here. Compared with traditional ocean wave direction inversion method, the ocean wave direction of derived by using this proposed method matches well with that measured by an in situ buoy nearby and the simulation data. These promising results demonstrate the efficiency and accuracy of the algorithm proposed in the paper.展开更多
In the multistage imaging processing for SAR digital imaging and applications ofSAR imagery,extraction of luminance edge for the SAR imageis often required.It is well studiedto extract the luminance edge for ordinary ...In the multistage imaging processing for SAR digital imaging and applications ofSAR imagery,extraction of luminance edge for the SAR imageis often required.It is well studiedto extract the luminance edge for ordinary images,The methods using gradient are effective andcommonly used.Because of the serious noise of coherent speckle exists in SAR images,somepeople believe that edge extraction by using gradient for SAR imagery gives poor results.Inthis paper,we have derived a rather ideal method for the extraction of luminance edge for SARimagery with the consideration of the characteristics of SAR imagery.This method uses therelative average gradient and combines detection with tracking.展开更多
This paper investigated approaches to supporting effective and efficient retrieval of image based on principle component analysis. First, it extracted the image content, texture and color. Gabor wavelet transforms wer...This paper investigated approaches to supporting effective and efficient retrieval of image based on principle component analysis. First, it extracted the image content, texture and color. Gabor wavelet transforms were used to extract texture feature of the image and the average color was used to extract the color features. The principle component of the feature vector of image can be constructed. Content based image retrieval was performed by comparing the feature vector of the query image with the projection feature vector of the image database on the principle component space of the query image. By this technique, it can reduce the dimensionality of feature vector, which in turn reduce the searching time.展开更多
Electrical Source Imaging (ESI) is a non-invasive technique of reconstructing brain activities using EEG data. This technique has been applied to evaluate epilepsy patients being evaluated for epilepsy surgery, showin...Electrical Source Imaging (ESI) is a non-invasive technique of reconstructing brain activities using EEG data. This technique has been applied to evaluate epilepsy patients being evaluated for epilepsy surgery, showing encouraging results for mapping interictal epileptiform discharges (IED). However, ESI is underused in planning epilepsy surgery. This is basically due to the wide availability of methods for solving the electromagnetism inverse problem (e-IP) associated to few studies using EEG setups similar to those most commonly used in clinical setting. In this study, we applied six different methods of solving the e-IP based on IEDs of 20 focal epilepsy patients that presented abnormalities in their MRI. We compared the ESI maps obtained by each method with the location of the abnormality, calculating the Euclidian distances from the center of the lesion to the closest border of the method solution (CL-BM) and also to the solution’s maxima (CL-MM). We also applied a score system in order to allow us to evaluate the sensitivity of each method for temporal and extra temporal patients. In our patients, the Bayesian Model Averaging method had a sensitivity of 86% and the shortest CL-MM. This method also had more restricted solutions that were more representative of epileptogenic activities than those obtained by the other methods.展开更多
This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the sha...This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the shapes are segregated into various regions according to its nature;otherwise, it is treated as textured image and considered the entire image as it is for the experiment. The aforesaid tests are applied regions-wise. First, the F-ratio test is applied, if the images pass the test, then it is proceeded to test the spectrum of energy, i.e. means of the two images. If the images pass both tests, then it is concluded that the two images are the same or similar. Otherwise, they differ. Since the proposed system is distribution-based, it is invariant for rotation and scaling. Also, the system facilitates the user to fix the number of images to be retrieved, because the user can fix the level of significance according to their requirements. These are the main advantages of the proposed system.展开更多
In this work, we propose an approach for the separation of coumarins from thin-layer morphological segmentation based on the acquisition of multicomponent images integrating different types of coumarins. The first ste...In this work, we propose an approach for the separation of coumarins from thin-layer morphological segmentation based on the acquisition of multicomponent images integrating different types of coumarins. The first step is to make a segmentation by region, by thresholding, by contour, etc. of each component of the digital image. Then, we proceeded to the calculations of parameters of the regions such as the color standard deviation, the color entropy, the average color of the pixels, the eccentricity from an algorithm on the matlab software. The mean color values at<sub>R</sub> = 91.20 in red, at<sub>B</sub> = 213.21 in blue showed the presence of samidin in the extract. The color entropy values H<sub>G</sub> = 5.25 in green and H<sub>B</sub> = 4.04 in blue also show the presence of visnadine in the leaves of Desmodium adscendens. These values are used to consolidate the database of separation and discrimination of the types of coumarins. The relevance of our coumarin separation or coumarin recognition method has been highlighted compared to other methods, such as the one based on the calculation of frontal ratios which cannot discriminate between two coumarins having the same frontal ratio. The robustness of our method is proven with respect to the separation and identification of some coumarins, in particular samidin and anglicine.展开更多
Diffusion tensor imaging is a sensitive way to reflect axonal necrosis and degeneration, glial cell regeneration and demyelination following spinal cord injury, and to display microstructure changes in the spinal cord...Diffusion tensor imaging is a sensitive way to reflect axonal necrosis and degeneration, glial cell regeneration and demyelination following spinal cord injury, and to display microstructure changes in the spinal cord in vivo. Diffusion tensor imaging technology is a sensitive method to diagnose spinal cord injury; fiber tractography visualizes the white matter fibers, and directly displays the structural integrity and resultant damage of the fiber bundle. At present, diffusion tensor imaging is restricted to brain examinations, and is rarely applied in the evaluation of spinal cord injury. This study aimed to explore the fractional anisotropy and apparent diffusion coefficient of diffusion tensor magnetic resonance imaging and the feasibility of diffusion tensor tractography in the evaluation of complete spinal cord injury in rats. The results showed that the average combined scores were obviously decreased after spinal cord transection in rats, and then began to increase over time. The fractional anisotropy scores after spinal cord transection in rats were significantly lower than those in normal rats (P 〈 0.05); the apparent diffusion coefficient was significantly increased compared with the normal group (P 〈 0.05). Following spinal cord transection, fractional anisotropy scores were negatively correlated with apparent diffusion coefficient values (r = -0.856, P 〈 0.01), and positively correlated with the average combined scores (r = 0.943, P 〈 0.01), while apparent diffusion coefficient values had a negative correlation with the average combined scores (r = -0.949, P 〈 0.01). Experimental findings suggest that, as a non-invasive examination, diffusion tensor magnetic resonance imaging can provide qualita- tive and quantitative information about spinal cord injury. The fractional anisotropy score and apparent diffusion coefficient have a good correlation with the average combined scores, which reflect functional recovery after spinal cord injury.展开更多
Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision lev...Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision level.Image processing techniques have dem-onstrated the remarkable capability to improve the SNR and the resolution level by breaking through some inherent limitations unresolved by instrument hardware optimization.In this paper, we focus on single-biomolecule imaging using atomic force mi-croscopy (AFM), a unique method in separated single-biomolecule imaging, and there was few suitable image processing tech-niques reported to increase the SNR of a single molecule’s AFM image.With the typical samples of separately dispersed DNA molecules, we replaced the classified averaging method, which is usually used when the molecules’ structure can be easily and repeatedly prepared, with the time averaging method to improve the SNR in a single molecule’s AFM imaging.Combining the time averaging technique with the image alignment method for the series of time-lapse AFM images of a single DNA molecule, we were able to improve the image’s SNR and recover some buried signals from the background noises.Furthermore, the fine structures of single molecules can potentially be further resolved if other image processing techniques are applied collaboratively with some newly developed imaging methods like Vibrating Mode Scanning Polarization Force Microscopy (VSPFM), and such combination will also be advantageous for the manipulation of single-biomolecules by AFM.In addition, the proposed algorithms for the estimations of drift, distortion and SNR are applicable for quantitative status characterization of AFM imaging.展开更多
In order to further improve the effectiveness of image processing,it is necessary that an efficient invariant representation is stable to deformation applied to images.This motivates the study of image representations...In order to further improve the effectiveness of image processing,it is necessary that an efficient invariant representation is stable to deformation applied to images.This motivates the study of image representations defining an Euclidean metric stable to these deformation.This paper mainly focuses on two aspects.On the one hand,in this paper,two properties of expected scattering and averaged scattering,i.e.,Lipschitz continuity and translation invariance,are proved in detail.These properties support that excepted scattering and averaged scattering are invariant,stable and informative representations.On the other hand,the issue of texture classification based on expected scattering and averaged scattering has been analyzed respectively in this study.Energy features,which are based on expected scattering and averaged scattering,are calculated and used for classification.Experimental results show that starting with the seventh feature,the two approaches can achieve good performance in texture image classification.展开更多
基金Supported by National Natural Science Foundation of China (No.30500129)
文摘In order to avoid the influence of noise variance on the filtering performances, a modified adaptive weighted averaging (MAWA) filtering algorithm is proposed for noisy image sequences. Based upon adaptive weighted averaging pixel values in consecutive frames, this algorithm achieves the filtering goal by assigning smaller weights to the pixels with inappropriate estimated motion trajectory for noise. It only utilizes the intensity of pixels to suppress noise and accordingly is independent of noise variance. To evaluate the performance of the proposed filtering algorithm, its mean square error and percentage of preserved edge points were compared with those of traditional adaptive weighted averaging and non-adaptive mean filtering algorithms under different noise variances. Relevant results show that the MAWA filtering algorithm can preserve image structures and edges under motion after attenuating noise, and thus may be used in image sequence filtering.
基金Princess Nourah bint Abdulrahman University and Researchers Supporting Project Number(PNURSP2024R346)Princess Nourah bint Abdulrahman University,Riyadh,Saudi Arabia.
文摘Recently,there have been several uses for digital image processing.Image fusion has become a prominent application in the domain of imaging processing.To create one final image that provesmore informative and helpful compared to the original input images,image fusion merges two or more initial images of the same item.Image fusion aims to produce,enhance,and transform significant elements of the source images into combined images for the sake of human visual perception.Image fusion is commonly employed for feature extraction in smart robots,clinical imaging,audiovisual camera integration,manufacturing process monitoring,electronic circuit design,advanced device diagnostics,and intelligent assembly line robots,with image quality varying depending on application.The research paper presents various methods for merging images in spatial and frequency domains,including a blend of stable and curvelet transformations,everageMax-Min,weighted principal component analysis(PCA),HIS(Hue,Intensity,Saturation),wavelet transform,discrete cosine transform(DCT),dual-tree Complex Wavelet Transform(CWT),and multiple wavelet transform.Image fusion methods integrate data from several source images of an identical target,thereby enhancing information in an extremely efficient manner.More precisely,in imaging techniques,the depth of field constraint precludes images from focusing on every object,leading to the exclusion of certain characteristics.To tackle thess challanges,a very efficient multi-focus wavelet decomposition and recompositionmethod is proposed.The use of these wavelet decomposition and recomposition techniques enables this method to make use of existing optimized wavelet code and filter choice.The simulated outcomes provide evidence that the suggested approach initially extracts particular characteristics from images in order to accurately reflect the level of clarity portrayed in the original images.This study enhances the performance of the eXtreme Gradient Boosting(XGBoost)algorithm in detecting brain malignancies with greater precision through the integration of computational image analysis and feature selection.The performance of images is improved by segmenting them employing the K-Means algorithm.The segmentation method aids in identifying specific regions of interest,using Particle Swarm Optimization(PCA)for trait selection and XGBoost for data classification.Extensive trials confirm the model’s exceptional visual performance,achieving an accuracy of up to 97.067%and providing good objective indicators.
文摘There is a transition region between objects and background in any gray image. Many valuable applications of image segmentation and edge detection based on transition region determination have been developed in recent years. But, the complexity of calculation for determining transition region is too high. It results in the very limitation of applications based on transition region. A new novel fast method for transition region determination is presented in this paper, which will reduce the complexity of calculation dramatically. Many experiments have showed that this algorithm is effective and correct and will lay a good foundation for applications based on transition region. Key words image segmentation - transition region - maximum point - efficient average gradient (EAG) CLC number TP 391.4 Biography: Zhang Ai-hua (1965-), male, Ph. D candidate, research direction: image processing.
文摘In this paper, a test system was developed in which a CCD camera was used as a sensor together with an IBM-AT-compatible computer with an Intel 80486 processor to measure the impurities and neps on a piece of cotton gray goods, and a method was proposed by which the differences of degree of gray between samples and interference caused by surface unevenness, creasing and the like within a test sample can be removed effectively. The whole test system is reliable, accurate and causing fewer subjective errors.
基金The National Key Research and Development Program of China under contract No.2016YFC0800405the Shanghai Municipal Science and Technology Project of China under contract No.15DZ0500600the Specialized Research Fund for the Doctoral Program of Higher Education of China under contract No.2014212020203
文摘As one of the important sea state parameters for navigation safety and coastal resource management, the ocean wave direction represents the propagation direction of the wave. A novel algorithm based on an optical flow method is developed for the ocean wave direction inversion of the ocean wave fields imaged by the X-band radar continuously. The proposed algorithm utilizes the echo images received by the X-band wave monitoring radar to estimate the optical flow motion, and then the actual wave propagation direction can be obtained by taking a weighted average of the motion vector for each pixel. Compared with the traditional ocean wave direction inversion method based on frequency-domain, the novel algorithm is fully using a time-domain signal processing method without determination of a current velocity and a modulation transfer function(MTF). In the meantime,the novel algorithm is simple, efficient and there is no need to do something more complicated here. Compared with traditional ocean wave direction inversion method, the ocean wave direction of derived by using this proposed method matches well with that measured by an in situ buoy nearby and the simulation data. These promising results demonstrate the efficiency and accuracy of the algorithm proposed in the paper.
文摘In the multistage imaging processing for SAR digital imaging and applications ofSAR imagery,extraction of luminance edge for the SAR imageis often required.It is well studiedto extract the luminance edge for ordinary images,The methods using gradient are effective andcommonly used.Because of the serious noise of coherent speckle exists in SAR images,somepeople believe that edge extraction by using gradient for SAR imagery gives poor results.Inthis paper,we have derived a rather ideal method for the extraction of luminance edge for SARimagery with the consideration of the characteristics of SAR imagery.This method uses therelative average gradient and combines detection with tracking.
文摘This paper investigated approaches to supporting effective and efficient retrieval of image based on principle component analysis. First, it extracted the image content, texture and color. Gabor wavelet transforms were used to extract texture feature of the image and the average color was used to extract the color features. The principle component of the feature vector of image can be constructed. Content based image retrieval was performed by comparing the feature vector of the query image with the projection feature vector of the image database on the principle component space of the query image. By this technique, it can reduce the dimensionality of feature vector, which in turn reduce the searching time.
文摘Electrical Source Imaging (ESI) is a non-invasive technique of reconstructing brain activities using EEG data. This technique has been applied to evaluate epilepsy patients being evaluated for epilepsy surgery, showing encouraging results for mapping interictal epileptiform discharges (IED). However, ESI is underused in planning epilepsy surgery. This is basically due to the wide availability of methods for solving the electromagnetism inverse problem (e-IP) associated to few studies using EEG setups similar to those most commonly used in clinical setting. In this study, we applied six different methods of solving the e-IP based on IEDs of 20 focal epilepsy patients that presented abnormalities in their MRI. We compared the ESI maps obtained by each method with the location of the abnormality, calculating the Euclidian distances from the center of the lesion to the closest border of the method solution (CL-BM) and also to the solution’s maxima (CL-MM). We also applied a score system in order to allow us to evaluate the sensitivity of each method for temporal and extra temporal patients. In our patients, the Bayesian Model Averaging method had a sensitivity of 86% and the shortest CL-MM. This method also had more restricted solutions that were more representative of epileptogenic activities than those obtained by the other methods.
文摘This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the shapes are segregated into various regions according to its nature;otherwise, it is treated as textured image and considered the entire image as it is for the experiment. The aforesaid tests are applied regions-wise. First, the F-ratio test is applied, if the images pass the test, then it is proceeded to test the spectrum of energy, i.e. means of the two images. If the images pass both tests, then it is concluded that the two images are the same or similar. Otherwise, they differ. Since the proposed system is distribution-based, it is invariant for rotation and scaling. Also, the system facilitates the user to fix the number of images to be retrieved, because the user can fix the level of significance according to their requirements. These are the main advantages of the proposed system.
文摘In this work, we propose an approach for the separation of coumarins from thin-layer morphological segmentation based on the acquisition of multicomponent images integrating different types of coumarins. The first step is to make a segmentation by region, by thresholding, by contour, etc. of each component of the digital image. Then, we proceeded to the calculations of parameters of the regions such as the color standard deviation, the color entropy, the average color of the pixels, the eccentricity from an algorithm on the matlab software. The mean color values at<sub>R</sub> = 91.20 in red, at<sub>B</sub> = 213.21 in blue showed the presence of samidin in the extract. The color entropy values H<sub>G</sub> = 5.25 in green and H<sub>B</sub> = 4.04 in blue also show the presence of visnadine in the leaves of Desmodium adscendens. These values are used to consolidate the database of separation and discrimination of the types of coumarins. The relevance of our coumarin separation or coumarin recognition method has been highlighted compared to other methods, such as the one based on the calculation of frontal ratios which cannot discriminate between two coumarins having the same frontal ratio. The robustness of our method is proven with respect to the separation and identification of some coumarins, in particular samidin and anglicine.
基金financially supported by a grant from the Shaanxi Provincial Science and Technology Research and Development Project,No.2013K12-20-08
文摘Diffusion tensor imaging is a sensitive way to reflect axonal necrosis and degeneration, glial cell regeneration and demyelination following spinal cord injury, and to display microstructure changes in the spinal cord in vivo. Diffusion tensor imaging technology is a sensitive method to diagnose spinal cord injury; fiber tractography visualizes the white matter fibers, and directly displays the structural integrity and resultant damage of the fiber bundle. At present, diffusion tensor imaging is restricted to brain examinations, and is rarely applied in the evaluation of spinal cord injury. This study aimed to explore the fractional anisotropy and apparent diffusion coefficient of diffusion tensor magnetic resonance imaging and the feasibility of diffusion tensor tractography in the evaluation of complete spinal cord injury in rats. The results showed that the average combined scores were obviously decreased after spinal cord transection in rats, and then began to increase over time. The fractional anisotropy scores after spinal cord transection in rats were significantly lower than those in normal rats (P 〈 0.05); the apparent diffusion coefficient was significantly increased compared with the normal group (P 〈 0.05). Following spinal cord transection, fractional anisotropy scores were negatively correlated with apparent diffusion coefficient values (r = -0.856, P 〈 0.01), and positively correlated with the average combined scores (r = 0.943, P 〈 0.01), while apparent diffusion coefficient values had a negative correlation with the average combined scores (r = -0.949, P 〈 0.01). Experimental findings suggest that, as a non-invasive examination, diffusion tensor magnetic resonance imaging can provide qualita- tive and quantitative information about spinal cord injury. The fractional anisotropy score and apparent diffusion coefficient have a good correlation with the average combined scores, which reflect functional recovery after spinal cord injury.
基金supported by the National Basic Research Program of China (2006CB932505 and 2007CB936004)
文摘Enhancement of the SNR (signal to noise ratio) in single-molecule imaging is significantly important for improving image resolu-tion and distinguishing the fine structures of single molecules at a higher precision level.Image processing techniques have dem-onstrated the remarkable capability to improve the SNR and the resolution level by breaking through some inherent limitations unresolved by instrument hardware optimization.In this paper, we focus on single-biomolecule imaging using atomic force mi-croscopy (AFM), a unique method in separated single-biomolecule imaging, and there was few suitable image processing tech-niques reported to increase the SNR of a single molecule’s AFM image.With the typical samples of separately dispersed DNA molecules, we replaced the classified averaging method, which is usually used when the molecules’ structure can be easily and repeatedly prepared, with the time averaging method to improve the SNR in a single molecule’s AFM imaging.Combining the time averaging technique with the image alignment method for the series of time-lapse AFM images of a single DNA molecule, we were able to improve the image’s SNR and recover some buried signals from the background noises.Furthermore, the fine structures of single molecules can potentially be further resolved if other image processing techniques are applied collaboratively with some newly developed imaging methods like Vibrating Mode Scanning Polarization Force Microscopy (VSPFM), and such combination will also be advantageous for the manipulation of single-biomolecules by AFM.In addition, the proposed algorithms for the estimations of drift, distortion and SNR are applicable for quantitative status characterization of AFM imaging.
基金Supported by the Natural Science Foundation of China(11626239)the Foundation of Education Department of Henan Province(18A110037)
文摘In order to further improve the effectiveness of image processing,it is necessary that an efficient invariant representation is stable to deformation applied to images.This motivates the study of image representations defining an Euclidean metric stable to these deformation.This paper mainly focuses on two aspects.On the one hand,in this paper,two properties of expected scattering and averaged scattering,i.e.,Lipschitz continuity and translation invariance,are proved in detail.These properties support that excepted scattering and averaged scattering are invariant,stable and informative representations.On the other hand,the issue of texture classification based on expected scattering and averaged scattering has been analyzed respectively in this study.Energy features,which are based on expected scattering and averaged scattering,are calculated and used for classification.Experimental results show that starting with the seventh feature,the two approaches can achieve good performance in texture image classification.