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Comparison of evolution laws of stress and strain fields in hot rolling of titanium alloy large rings with different sizes 被引量:3
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作者 王敏 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2011年第7期1611-1619,共9页
For hot rolling of titanium alloy large rings,evolution laws of stress and strain fields in rings with various sizes were explored and compared based on a reliable coupled thermo-mechanical three-dimensional (3D) fi... For hot rolling of titanium alloy large rings,evolution laws of stress and strain fields in rings with various sizes were explored and compared based on a reliable coupled thermo-mechanical three-dimensional (3D) finite element (FE) model.The results show that for forming processes of different rings,as γ^-(the equivalent distribution ratio of feed amount per revolution of a process) decreases,the final peak Mises stress may transfer from the biting point at the driver roll side to that at the idle roll side,and the final peak equivalent plastic strain may transfer from the outside surface to the inside surface;as L^- (the equivalent deformation zone length of a process) increases,the final peak Mises stress may appear in the middle layer.The final positions of peak Mises stress and equivalent plastic strain are the combined effects of the above two aspects.In the deformation zone of a deformed ring,the surface layers are in the 3D compressive stress state,while the middle layer is in the 1D compressive and 2D tensile stress state or 2D compressive and 1D tensile stress state;the whole ring is in the 1D compressive and 2D tensile strain state. 展开更多
关键词 titanium alloy hot rolling of large rings stress and strain modeling and simulation finite element
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Measurement of residual stress in a multi-layer semiconductor heterostructure by micro-Raman spectroscopy 被引量:15
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作者 Wei Qiu Cui-Li Cheng +7 位作者 Ren-Rong Liang Chun-Wang Zhao Zhen-Kun Lei Yu-Cheng Zhao Lu-Lu Ma Jun Xu Hua-Jun Fang Yi-Lan Kang 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2016年第5期805-812,共8页
Si-based multilayer structures are widely used in current microelectronics. During their preparation, some inhomogeneous residual stress is induced, resulting in competition between interface mismatching and surface e... Si-based multilayer structures are widely used in current microelectronics. During their preparation, some inhomogeneous residual stress is induced, resulting in competition between interface mismatching and surface energy and even leading to structure failure. This work presents a methodological study on the measurement of residual stress in a multi-layer semiconductor heterostructure. Scanning electron microscopy(SEM), micro-Raman spectroscopy(MRS), and transmission electron microscopy(TEM) were applied to measure the geometric parameters of the multilayer structure. The relationship between the Raman spectrum and the stress/strain on the [100] and [110] crystal orientations was determined to enable surface and crosssection residual stress analyses, respectively. Based on the Raman mapping results, the distribution of residual stress along the depth of the multi-layer heterostructure was successfully obtained. 展开更多
关键词 Residual stress multi-layer semiconductor heterostructure Micro-Raman spectroscopy(MRS) Strained silicon Germanium silicon
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Analysis on diffusion-induced stress for multi-layer spherical core-shell electrodes in Li-ion batteries
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作者 Siyuan Yang Chuanwei Li +4 位作者 Zhifeng Qi Lipan Xin Linan Li Shibin Wang Zhiyong Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第9期587-593,共7页
Silicon-based carbon composites are believed as promising anodes in the near future due to their outstanding specific capacity and relatively lower volume effect compared to pure silicon anodes.Herein,a multilayer sph... Silicon-based carbon composites are believed as promising anodes in the near future due to their outstanding specific capacity and relatively lower volume effect compared to pure silicon anodes.Herein,a multilayer spherical core-shell(M-SCS)electrode with a graphite framework prepared with Si@O-MCMB/C nanoparticles is developed,which aims to realize chemically/mechanically stability during the lithiation/delithiation process with high specific capacity.An electrochemical-/mechanical-coupling model for the M-SCS structure is established with various chemical/mechanical boundary conditions.The simulation of finite difference method(FDM)has been conducted based on the proposed coupling model,by which the diffusion-induced stress along both the radial and the circumferential directions is determined.Moreover,factors that influence the diffusion-induced stress of the M-SCS structure have been discussed and analyzed in detail. 展开更多
关键词 multi-layer spherical core-shell electrode diffusion-induced stress
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STRESS ANALYSIS OF ROLL RING FOR HIGH SPEED WIRE ROLLING MILLS
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作者 王邦文 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 1995年第1期30+24-30,共8页
This paper introduces a new method for fixing type of the tungsten carbide roll ring for high speed wire rolling mills, and analyses the stresses of the roll ring. The lifetime of roll pass is twice longer that of the... This paper introduces a new method for fixing type of the tungsten carbide roll ring for high speed wire rolling mills, and analyses the stresses of the roll ring. The lifetime of roll pass is twice longer that of the old one. 展开更多
关键词 wire rolling mills tungsten carbide lifetimes/roll ring thermal stress
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Finite element analysis of stress distribution and burst failure of SiC_f/Ti-6Al-4V composite ring 被引量:2
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作者 张红园 杨延清 罗贤 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第1期261-270,共10页
A three-dimensional cyclic symmetry finite element model of titanium-matrix composites(TMCs) ring was developed to investigate the stress distribution and burst failure. The effects of fiber volume fractions, reinfo... A three-dimensional cyclic symmetry finite element model of titanium-matrix composites(TMCs) ring was developed to investigate the stress distribution and burst failure. The effects of fiber volume fractions, reinforced areas, thermal residual stresses and two different temperatures on stress distribution were studied. The burst speed was obtained through analyzing the hoop tensile stresses under a series of rotating speeds. The results indicate that at the two different temperatures, the influences of fiber volume fractions and reinforced areas on stress level and distribution are different. Some proposals are provided for the structure design of the TMCs ring. With regard to thermal residual stresses, a larger reinforced area is an advisable choice for design of the ring at higher temperature. 展开更多
关键词 titanium-matrix composites ring stress distribution burst failure finite element analysis thermal residual stresses
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Optimum Design for Shrink-fit Multi-layer Vessels under Ultrahigh Pressure Using Different Materials 被引量:6
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作者 YUAN Gexia LIU Hongzhao WANG Zhongrnin 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2010年第5期582-589,共8页
Multi-layer pressure vessels are widely used in every field of high pressure technology.For the purpose of enhancing a vessels' load bearing capacity,a beneficial process like shrink-fit is usually employed.However,f... Multi-layer pressure vessels are widely used in every field of high pressure technology.For the purpose of enhancing a vessels' load bearing capacity,a beneficial process like shrink-fit is usually employed.However,few documents on optimum design for multi-layer shrink-fit vessels made of different strength materials can be found,available data are mainly on two-layer vessels.In this paper,an optimum design approach is developed for shrink-fit multi-layer vessels under ultrahigh pressure by using different materials.Maximum shear stress theory is applied as design criteria.The inner and outer radii of a multi-layer vessel,as well as the material of each layer,are assumed to be known.The optimization mathematical model is,thereby,built.Lagrange multipliers method is required to obtain the optimal design formula of wall ratio(ratio of outer to inner radii) of each layer,from which the optimum formulas of shrinkage pressure and radial interference are derived with the superposition principle employed.These formulas are applicable for the optimization design of all multi-layer vessels made of different materials,or same materials.The formulas of the limit working pressure and the contact pressure show that the optimum wall ratio of each layer and limit working pressure are only related to all selected material strength and unrelated to the position of the layer placement in the vessel.However,shrinkage pressure is related to the position of the layer placement in the vessel.Optimization design of an open ended shrink-fit three-layer vessel using different materials and comparisons proved that the optimized multi-layer vessels have outstanding characteristics of small radial interference and are easier for assembly.When the stress of each layer is distributed more evenly and appropriately,the load bearing capability and safety of vessels are enhanced.Therefore,this design is material-saving and cost-effective,and has prospect of engineering application. 展开更多
关键词 multi-layer vessels Lagrange multiplier shrink-fit optimum interference maximum shear stress theory
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Energy Absorption Diagrams of Multi-layer Corrugated Boards 被引量:5
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作者 王冬梅 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2010年第1期58-61,共4页
Based on the static compression experiments, the compressive stress-strain curve of multi-layer corrugated boards is simplified into three sections of linear elasticity, sub-buckling going with local collapse and dens... Based on the static compression experiments, the compressive stress-strain curve of multi-layer corrugated boards is simplified into three sections of linear elasticity, sub-buckling going with local collapse and densification. By considering the structure factors of multi-layer corrugated boards, the energy absorption model is obtained and characterized by the structure factors of corrugated cell-wall. The model is standardized by the solid modulus and it is universal for corrugated structures of different basis material. In the liner-elastic section, with the increase of the load, the energy absorption per unit volume of multi-layer corrugated boards gradually increases; in the sub-buckling section going with local collapse, the compression resistance of multi-layer corrugated boards goes on under a nearly constant load, but the energy absorption per unit volume rapidly increases with the increase of the compression strain. It is shown as an ascending curve in the energy absorption diagram. In the densification section, the corrugated sandwich core has no energy absorption capability. A good consistency is achieved between theoretical and experimental energy absorption curves. In designing the cushioning package, the cushioning properties can be evaluated by the theoretical model without more experiments. The suggested method to develop the energy absorption diagram for corrugated boards can be used to characterize the cushioning properties and optimize the structures of corrugated sandwich structures. 展开更多
关键词 multi-layer corrugated boards stress strain curve energy absorption diagram MODELLING
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Effects of cartilaginous rings on airflow and particle transport through simplified and realistic models of human upper respiratory tracts 被引量:1
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作者 Vivek Kumar Srivastav Akshoy Ranjan Paul Anuj Jain 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2013年第6期883-892,共10页
In the present study, computational fluid dynamics (CFD) is used to investigate inspiratory and expiratory airflow characteristics in the human upper respiratory tract for the purpose of identifying the probable loc... In the present study, computational fluid dynamics (CFD) is used to investigate inspiratory and expiratory airflow characteristics in the human upper respiratory tract for the purpose of identifying the probable locations of particle deposition and the wall injury. Computed tomography (CT) scan data was used to reconstruct a three dimensional respiratory tract from trachea to first generation bronchi. To compare, a simplified model of respiratory tract based on Weibel was also used in the study. The steady state results are obtained for an airflow rate of 45 L/min, corresponding to the heavy breathing condition. The velocity distribution, wall shear stress, static pressure and particle deposition are compared for inspiratory flows in simplified and realistic models and expiratory flows in realistic model only. The results show that the location of cartilaginous rings is susceptible to wall injury and local particle deposition. 展开更多
关键词 Upper respiratory tract Cartilaginous rings Computational fluid dynamics (CFD) Computed tomogra- phy (CT) Wall shear stress Particle deposition.
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A Reverse Numerical Approach to Determine Elastic-plastic Properties of Multi-layer Material Systems with Flat Cylindrical Indenters 被引量:1
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作者 Baoxing XU Zhufeng YUE 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2007年第5期707-712,共6页
In the present study, the indentation testing with a flat cylindrical indenter on typical multi-layer material systems was simulated successfully by finite element method. The emphasis was put on the methods of extrac... In the present study, the indentation testing with a flat cylindrical indenter on typical multi-layer material systems was simulated successfully by finite element method. The emphasis was put on the methods of extracting the yield stresses and strain-hardening modulus of upper and middle-layers of three-layer material systems from the indentation testing. The slope of the indentation depth to the applied indentation stress curve was found to have a turning point, which can be used to determine the yield stress of the upper-layer. Then, a different method was also presented to determine the yield stress of the middle-layer. This method was based on a set of assumed applied indentation stresses which were to be intersected by the experimental results in order to meet the requirement of having the experimental indentation depth. At last, a reverse numerical algorithm was explored to determine the yield stresses of upper and middle-layers simultaneously by using the indentation testing with two different size indenters. This method assumed two ranges of yield stresses to simulate the indentation behavior. The experimental depth behavior was used to intersect the simulated indentation behavior. And the intersection corresponded to the values of yield stresses of upper and middle-layers. This method was also used further to determine the strain-hardening modulus of upper and middle-layers simultaneously. 展开更多
关键词 multi-layer material systems Indentation testing Finite element method (FEM) Yield stress Strain-hardening modulus
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Using Neural Network to Predict the Interfacial Friction and Flow Stress of Materials via Ring Compression
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作者 Xu Weiliang (Department of Mechanical Engineering, Southeast University, Nanjing 210018) Kamineni P. Rao(City University of Hong Kong, Hong Kong) 《Journal of Southeast University(English Edition)》 EI CAS 1996年第1期53-60,共8页
In this study a neural network approach is proposed to realize an automatic numerical prediction of the interfacial friction factor and the flow stress of materials. Decrease in the inner diameter and reduction in the... In this study a neural network approach is proposed to realize an automatic numerical prediction of the interfacial friction factor and the flow stress of materials. Decrease in the inner diameter and reduction in the height of the ring are taken as input 展开更多
关键词 neural networks metal FORMING FRICTION PREDICTION flow stress PREDICTION ring compression test
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A spatiotemporal signal processing technique for wafer-scale IC thermomechanical stress monitoring by an infrared camera
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作者 Michel Saydé Ahmed Lakhssassi +1 位作者 Emmanuel Kengne Roman Palenichka 《Journal of Biosciences and Medicines》 2013年第2期1-5,共5页
In this paper, we describe a new silicon-die thermal monitoring approach using spatiotemporal signal processing technique for Wafer-Scale IC thermome- chanical stress monitoring. It is proposed in the context of a waf... In this paper, we describe a new silicon-die thermal monitoring approach using spatiotemporal signal processing technique for Wafer-Scale IC thermome- chanical stress monitoring. It is proposed in the context of a wafer-scale-based (WaferICTM) rapid prototyping platform for electronic systems. This technique will be embedded into the structure of the WaferIC, and will be used as a preventive measure to protect the wafer from possible damages that can be caused by excessive thermomechanical stress. The paper also presents spatial and spatiotemporal algorithms and the experimental results from an IR images collection campaign conducted using an IR camera. 展开更多
关键词 THERMAL Monitoring ring Oscillator (RO) Spatial SPATIOTEMPORAL THERMO-MECHANICAL stress Temperature Sensor THERMAL Analysis WaferIC Wafer-Scale System
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Dynamics of the Moving Ring Load Acting in the System“Hollow Cylinder+Surrounding Medium”with Inhomogeneous Initial Stresses
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作者 Surkay DAkbarov Emin TBagirov Muslum Ozisik 《Computers, Materials & Continua》 SCIE EI 2019年第8期503-534,共32页
This paper studies the influence of the inhomogeneous initial stress state in the system consisting of a hollow cylinder and surrounding elastic medium on the dynamics of the moving ring load acting in the interior of... This paper studies the influence of the inhomogeneous initial stress state in the system consisting of a hollow cylinder and surrounding elastic medium on the dynamics of the moving ring load acting in the interior of the cylinder.It is assumed that in the initial state the system is compressed by uniformly distributed normal forces acting at infinity in the radial inward direction and as a result of this compression the inhomogeneous initial stresses appear in the system.After appearance of the initial stresses,the interior of the hollow cylinder is loaded by the moving ring load and so it is required to study the influence of the indicated inhomogeneous initial stresses on the dynamics of this moving load.This influence is studied with utilizing the so-called threedimensional linearized theory of elastic waves in elastic bodies with initial stresses.For solution of the corresponding mathematical problems,the discrete-analytical solution method is employed and the approximate analytical solution of these equations is achieved.Numerical results obtained within this method and related to the influence of the inhomogeneous initial stresses on the critical velocity of the moving load and on the response of the interface stresses to this load are presented and discussed.In particular,it is established that the initial inhomogeneous initial stresses appearing as a result of the action of the aforementioned compressional forces cause to increase the values of the critical velocity of the moving load. 展开更多
关键词 Inhomogeneous initial stresses dynamics of a moving load critical velocity hollow cylinder elastic medium ring load
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Characterization and expression analysis of a novel RING-HC gene,ZmRHCP1,involved in brace root development and abiotic stress responses in maize 被引量:1
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作者 LI Wen-lan SUN Qi +3 位作者 LI Wen-cai YU Yan-li ZHAO Meng MENG Zhao-dong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第9期1892-1899,共8页
RING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the Zm RHCP1 gene encoding a putative R... RING is a really interesting new gene which plays important regulatory roles in many developmental processes as well as in plant-environment interactions. In the present report, the Zm RHCP1 gene encoding a putative RING-HC protein was isolated from maize and characterized. The Zm RHCP1 protein contained 310 amino acid residues with a conserved RINGHC zinc-finger motif and two transmembrane(TM) domains. Zm RHCP1 was expressed ubiquitously in various organs(root, stem, leaf, seedling, immature ear, and tassel), but its transcript levels were higher in vegetative organs than in reproductive organs. Moreover, the expression pattern of Zm RHCP1 in brace roots indicated that Zm RHCP1 functions in brace root initiation. In addition, Zm RHCP1 expression was regulated by abiotic stresses. The expression results suggested that Zm RHCP1 plays important roles in brace root development and abiotic stress responses. The findings of the present study provide important information to help us understand the function of Zm RHCP1 in maize. 展开更多
关键词 ring-HC zinc-finger brace root expression abiotic stresses maize
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Residual stress in the cylindrical drawing cup of SUS304 stainless steel evaluated by split-ring test
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作者 Liang-Hong Xiao Dao-He Yuan +2 位作者 Jun-Zhong Xiang Jin-Gang Liu Yi-Chun Zhou 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2016年第1期125-134,共10页
The residual stresses in the wall of a SUS304 stainless steel cylindrical drawing cup were evaluated by split-ring tests, and the influences of stamping die parame- ters on the residual stress were investigated. A new... The residual stresses in the wall of a SUS304 stainless steel cylindrical drawing cup were evaluated by split-ring tests, and the influences of stamping die parame- ters on the residual stress were investigated. A new theoretical model of a split-ring test was developed to evaluate the resid- ual stress in a ring, which was verified to be reasonable and reliable by numerical simulations with ABAQUS code and by nanoindentation tests. Seven groups of split-ring tests were completed, and the residual stresses were calculated according to the theoretical model. The split-ring test results showed that the circumferential residual stress in the wall of the SUS304 stainless steel cylindrical drawing cup was very large and did not change with the different die comer radius. The circumferential residual stress first increased with the increase of drawing punch-die clearance, then was almost unchanged when the clearance increased greater than blank thickness 1 mm. Thus, a smaller clearance was suggested to be chosen to reduce the residual stress in the wall of the SUS304 stainless steel drawing cup. 展开更多
关键词 Residual stress Split-ring test FEM numericalsimulation SUS304 stainless steel NANOINDENTATION
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Dynamics of perinuclear actin ring regulating nuclear morphology
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作者 Haoxiang YANG Houbo SUN +2 位作者 Jinghao SHEN Hao WU Hongyuan JIANG 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2024年第8期1415-1428,共14页
Cells are capable of sensing and responding to the extracellular mechanical microenvironment via the actin skeleton.In vivo,tissues are frequently subject to mechanical forces,such as the rapid and significant shear f... Cells are capable of sensing and responding to the extracellular mechanical microenvironment via the actin skeleton.In vivo,tissues are frequently subject to mechanical forces,such as the rapid and significant shear flow encountered by vascular endothelial cells.However,the investigations about the transient response of intracellular actin networks under these intense external mechanical forces,their intrinsic mechanisms,and potential implications are very limited.Here,we observe that when cells are subject to the shear flow,an actin ring structure could be rapidly assembled at the periphery of the nucleus.To gain insights into the mechanism underlying this perinuclear actin ring assembly,we develop a computational model of actin dynamics.We demonstrate that this perinuclear actin ring assembly is triggered by the depolymerization of cortical actin,Arp2/3-dependent actin filament polymerization,and myosin-mediated actin network contraction.Furthermore,we discover that the compressive stress generated by the perinuclear actin ring could lead to a reduction in the nuclear spreading area,an increase in the nuclear height,and a decrease in the nuclear volume.The present model thus explains the mechanism of the perinuclear actin ring assembly under external mechanical forces and suggests that the spontaneous contraction of this actin structure can significantly impact nuclear morphology. 展开更多
关键词 mechanical force actin dynamics perinuclear actin ring compressive stress NUCLEUS
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铁观音茶树RING型E3泛素连接酶基因克隆及其干旱胁迫下的表达分析 被引量:1
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作者 常笑君 郭玉琼 +4 位作者 王仲 赵姗姗 朱晨 林玉玲 赖钟雄 《福建农业学报》 CAS 北大核心 2016年第8期826-832,共7页
以铁观音茶树为试材,对其中E3泛素连接酶基因进行克隆和生物信息学分析,并采用qPCR进行不同干旱条件下的定量表达分析。研究结果表明,该序列全长为1 138bp,开放阅读框(ORF)为810bp,编码269个氨基酸(GenBank登录号KR819177)。生物信息学... 以铁观音茶树为试材,对其中E3泛素连接酶基因进行克隆和生物信息学分析,并采用qPCR进行不同干旱条件下的定量表达分析。研究结果表明,该序列全长为1 138bp,开放阅读框(ORF)为810bp,编码269个氨基酸(GenBank登录号KR819177)。生物信息学分析发现该铁观音茶树E3泛素连接酶基因不含跨膜结构以及信号肽,具有多个磷酸化位点,亚细胞定位于叶绿体中。经BLAST比对,该基因编码的氨基酸序列与烟草、亚麻荠、葡萄、醉蝶花、芜菁中的E3泛素连接酶基因编码的氨基酸序列分别有51%、50%、50%、50%和49%的同源性,且相关保守功能结构域翻译的蛋白质序列具有RING-finger结构,初步确定该基因为铁观音茶树的E3泛素连接酶基因。qPCR分析结果显示在不同干旱胁迫处理下铁观音茶树的E3泛素连接酶基因的表达量,与对照组相比显著增加。本研究认为铁观音茶树RING型E3泛素连接酶基因参与茶树抗旱响应机制。 展开更多
关键词 铁观音 ring型E3泛素连接酶 基因克隆 干旱胁迫 qPCR
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橡胶O形密封圈的加速老化法寿命预估
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作者 刘学通 杨敬亭 +4 位作者 王美芳 宫志欣 胡洁 刘国庆 赵鑫 《橡胶工业》 CAS 2025年第1期66-69,共4页
以丁腈橡胶O形橡胶密封圈(简称橡胶O形圈)为例,提出加速老化法预估橡胶O形圈的寿命的一种新方法。以压缩应力松弛性能保持率为预估指标,分别在80,100,110和120℃下对橡胶O形圈进行压缩应力松弛试验,先推算出橡胶O形圈贮存3,5,10,15和20... 以丁腈橡胶O形橡胶密封圈(简称橡胶O形圈)为例,提出加速老化法预估橡胶O形圈的寿命的一种新方法。以压缩应力松弛性能保持率为预估指标,分别在80,100,110和120℃下对橡胶O形圈进行压缩应力松弛试验,先推算出橡胶O形圈贮存3,5,10,15和20年的压缩应力松弛性能保持率,再推算出在80和100℃下橡胶O形圈贮存3,5,10,15和20年所需的加速老化时间。 展开更多
关键词 丁腈橡胶 O形密封圈 压缩应力松弛性能保持率 加速老化法 寿命
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小麦RING型E3泛素连接酶基因TaSDIR1-D克隆与功能分析 被引量:6
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作者 王瑞同 王景一 +3 位作者 毛新国 昌小平 刘惠民 景蕊莲 《植物遗传资源学报》 CAS CSCD 北大核心 2018年第5期951-958,共8页
具有RING结构域的E3泛素连接酶SDIR1(Salt-and drought induced really interesting new gene finger1)在植物信号调节通路中发挥着重要的作用。本研究克隆得到小麦TaSDIR1-D基因,基因组序列全长4070 bp,其中编码区上游为1443 bp,编码区... 具有RING结构域的E3泛素连接酶SDIR1(Salt-and drought induced really interesting new gene finger1)在植物信号调节通路中发挥着重要的作用。本研究克隆得到小麦TaSDIR1-D基因,基因组序列全长4070 bp,其中编码区上游为1443 bp,编码区为2352 bp,3'端非编码区(UTR)为275 bp;该基因编码区cDNA序列长度为849 bp,预测其编码282个氨基酸,包括2个跨膜结构域和1个保守的RING型finger结构域。以野生近缘种的二倍体、四倍体和六倍体普通小麦及一套由中国春为背景的缺-四体为材料,将基因定位在染色体4D上;小麦抽穗期TaSDIR1-D在旗叶中的表达量最高;在NaCl、ABA、PEG及4℃非生物胁迫诱导下,小麦TaSDIR1-D均上调表达,可能参与植物抗逆调节通路;通过检测32份多态性较高的小麦材料TaSDIR1-D基因序列多态性,共检测到2个SNP,基因的编码区和上游序列中各存在1个核苷酸变异,其中编码区的核苷酸变异位点(G/A)位于第4外显子上,为非同义突变,使精氨酸(Agr)变为组氨酸(His)。2个SNP组成两种单倍型,根据-583 bp处的SNP(T/C)设计分子标记SNP-583,扫描自然群体262份材料的基因型,将其分为2种单倍型,分别与千粒重、倒二节长和穗长显著相关或极显著相关,单倍型Hap-4D-2为提高千粒重的优异单倍型。研究结果为小麦分子育种提供了理论依据和基因资源。 展开更多
关键词 TaSDIR1-D ring型E3泛素连接酶 抗逆性 分子标记 关联分析
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植物非生物逆境胁迫相关RING finger蛋白 被引量:2
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作者 刘丽娟 刘爱玲 陈信波 《生物技术通报》 CAS CSCD 北大核心 2014年第7期1-7,共7页
RING finger蛋白是锌指蛋白类中一个庞大的蛋白家族,已成为拟南芥第三大蛋白家族,在水稻中也发现了488个。最典型的结构特点是序列内包含环指结构域(RING finger domain)。RING finger蛋白主要通过泛素化途径参与到植物细胞的生理生化... RING finger蛋白是锌指蛋白类中一个庞大的蛋白家族,已成为拟南芥第三大蛋白家族,在水稻中也发现了488个。最典型的结构特点是序列内包含环指结构域(RING finger domain)。RING finger蛋白主要通过泛素化途径参与到植物细胞的生理生化过程。介绍了植物RING finger蛋白的结构特点和分类,综述它们的亚细胞定位,重点阐述它们在响应干旱胁迫、温度胁迫和盐胁迫等非生物逆境胁迫的调控作用。 展开更多
关键词 ring finger蛋白 非生物逆境胁迫 泛素连接酶E3
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大豆RING/U-box蛋白Glyma.13G115900的克隆及其对非生物胁迫的应答 被引量:2
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作者 张沿政 陈龙 +1 位作者 李永光 李文滨 《大豆科学》 CAS CSCD 北大核心 2017年第6期851-856,共6页
课题组前期对干旱胁迫下大豆转录组的数据分析发现大豆Glyma.13G115900基因编码一个RING/U-box蛋白,其表达水平受干旱胁迫影响显著。本研究以垦丰16大豆为试验材料,克隆Glyma.13G115900基因。氨基酸多重序列比对表明其编码的蛋白与其它... 课题组前期对干旱胁迫下大豆转录组的数据分析发现大豆Glyma.13G115900基因编码一个RING/U-box蛋白,其表达水平受干旱胁迫影响显著。本研究以垦丰16大豆为试验材料,克隆Glyma.13G115900基因。氨基酸多重序列比对表明其编码的蛋白与其它物种都具有高度保守的RING/U-box结构域。构建原核表达载体pET-29b-Glyma.13G115900转化到大肠杆菌中,Glyma.13G115900蛋白在大肠杆菌中能够表达。荧光定量PCR分析表明Glyma.13G115900基因的表达量受PEG、NaCl和ABA的影响显著,但基本不受冷胁迫的诱导。经PEG和NaCl处理后,该基因表达量与CK相比呈现出显著下降的趋势,PEG处理的表达量变化比NaCl下调的更明显;在ABA诱导下与CK相比该基因的mRNA丰度呈现出先上升后下降的趋势,在4 h表达量出现峰值,推测该基因可能通过依赖于ABA途径参与非生物胁迫应答。以上结果为进一步深入研究该基因的调控途径奠定基础。 展开更多
关键词 大豆 ring/U-box蛋白 Glyma.13G115900 基因克隆 胁迫响应
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