Deep neural network based on multi-level wavelet and attention for structured illumination microscopy

Structured illumination microscopy(SIM)is a popular and powerful super-resolution(SR)technique in biomedical research.However,the conventional reconstruction algorithm for SIM heavily relies on the accurate prior knowledge of illumination patterns and signal-to-noise ratio(SNR)of raw images.To obtain high-quality SR images,several raw images need to be captured under high fluorescence level,which further restricts SIM’s temporal resolution and its applications.Deep learning(DL)is a data-driven technology that has been used to expand the limits of optical microscopy.In this study,we propose a deep neural network based on multi-level wavelet and attention mechanism(MWAM)for SIM.Our results show that the MWAM network can extract high-frequency information contained in SIM raw images and accurately integrate it into the output image,resulting in superior SR images compared to those generated using wide-field images as input data.We also demonstrate that the number of SIM raw images can be reduced to three,with one image in each illumination orientation,to achieve the optimal tradeoff between temporal and spatial resolution.Furthermore,our MWAM network exhibits superior reconstruction ability on low-SNR images compared to conventional SIM algorithms.We have also analyzed the adaptability of this network on other biological samples and successfully applied the pretrained model to other SIM systems.

Selected abstracts of the symposium onenvironmental protection and ecologyin developing countries

Nov. 1—4, 1989, Beijing, China River water chemistry in India-An overview V. Subramamian School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Based on extensive analyses of a very large number of samples, the average river water in India is more alkaline than the world average river water. The dominance of Na and Cl in Indian river shows their monsoon control. There are spatial and seasonal variations. The northern river are less saline than the southern rivers. The sediments covered by the Ganges-

Study on the instantaneous protection reliability of low voltage circuit breakers

This paper outlines the significance of enhancing the instantaneous protection reliability of low voltage circuit breakers and describes their main failure modes. The instantaneous failure mechanism of low voltage circuit breakers was analyzed so that measures to improve instantaneous protection reliability can be determined. Furthermore, the theory of the instantaneous characteristics calibration device for low voltage circuit breakers and the method of eliminating the non-periodic component of test current are given in detail. Finally, the test results are presented.

Rural Migrant Workers' Welfare and Labor Protection in China under the Labor Contract Law

This paper employs difference-in-differences(DID) approach to evaluate the effects of China's Labor Contract Law's implementation on rural migrant workers' welfare.Our findings suggest that the Labor Contract Law has reduced rural migrant workers' working hours by 23%, and increased their social insurance coverage by 10% to 26%. This conclusion holds true after removal of sample selection bias and policy expectation factor.Further analysis reveals that Labor Contract Law's welfare improvement effect was more significant for migrant workers in regions where workers had poor bargaining power. Other economic factors during the same period of time did not affect the law's labor protection effect. Our findings give a clear answer to controversies over whether the Labor Contract Law can improve labor rights for underprivileged groups, and are of reference value for developing labor protection systems.

Innovative Design and Additive Manufacturing of Regenerative Cooling Thermal Protection System Based on the Triply Periodic Minimal Surface Porous Structure

The new regenerative cooling thermal protection system exhibits the multifunctional characteristics of load-carrying and heat exchange cooling,which are fundamental for the lightweight design and thermal protection of hypersonic vehicles.Triply periodic minimal surface(TPMS)is especially suitable for the structural design of the internal cavity of regenerative cooling structures owing to its excellent structural characteristics.In this study,test pieces were manufactured using Ti6Al4V lightweight material.We designed three types of porous test pieces,and the interior was filled with a TPMS lattice(Gyroid,Primitive,I-WP)with a porosity of 30%.All porous test pieces were manufactured via selective laser melting technology.A combination of experiments and finite element simulations were performed to study the selection of the internal cavity structure of the regenerative cooling thermal protection system.Hence,the relationship between the geometry and mechanical properties of a unit cell is established,and the deformation mechanism of the porous unit cell is clarified.Among the three types of porous test pieces,the weight of the test piece filled with the Gyroid unit cell was reduced by 8.21%,the average tensile strength was reduced by 17.7%compared to the solid test piece,while the average tensile strength of the Primitive and I-WP porous test pieces were decreased by 30.5%and 33.3%,respectively.Compared with the other two types of unit cells,Gyroid exhibited better mechanical conductivity characteristics.Its deformation process was characterised by stretching,shearing,and twisting,while the Primitive and I-WP unit cells underwent tensile deformation and tensile and shear deformation,respectively.The finite element predictions in the study agree well with the experimental results.The results can provide a basis for the design of regenerative cooling thermal protection system.

Protection of Distributed Generation: Challenges and Solutions

Distributed generation (DG) is the future of energy. This technology allows the bidirectional flow of power within an electrical network. Researchers are faced with many challenges to the accurate implementation of protection schemes for DG-connected distribution network. The schemes designed must satisfy the performance requirements of selectivity, reliability, and sensitivity. Most researchers opine that conventional protection schemes based on over current detection are insufficient to completely and accurately protect a DG-connected distributed power system. There are many challenges?that?need to be tackled before embarking upon the journey to successfully implement these schemes. This paper summarizes the major challenges which one can encounter while designing protection schemes for DG-connected distribution networks. Some possible solutions from the literature are also mentioned. Moreover, a suggested solution for protecting future active distribution networks is provided. It is expected that this paper will act as a benchmark for future researchers in this field to tackle the challenges related to the protection of active distribution networks.

Excitatory amino acid changes in the brains of rhesus monkeys following selective cerebral deep hypothermia and blood flow occlusion

Selective cerebral deep hypothermia and blood flow occlusion can enhance brain tolerance to ischemia and hypoxia and reduce cardiopulmonary complications in monkeys. Excitotoxicity induced by the release of a large amount of excitatory amino acids after cerebral ischemia is the major mechanism underlying ischemic brain injury and nerve cell death. In the present study, we used selective cerebral deep hypothermia and blood flow occlusion to block the bilateral common carotid arteries and/or bilateral vertebral arteries in rhesus monkey, followed by reperfusion using Ringer＇s solution at 4~C. Microdialysis and transmission electron microscope results showed that selective cerebral deep hypothermia and blood flow occlusion inhibited the release of glutamic acid into the extracellular fluid in the brain frontal lobe and relieved pathological injury in terms of the ultrastructure of brain tissues after severe cerebral ischemia. These findings indicate that cerebral deep hypothermia and blood flow occlusion can inhibit cytotoxic effects and attenuate ischemic/ hypoxic brain injury through decreasing the release of excitatory amino acids, such as glutamic acid.

Highly Selective Preparation of 3-and 6-Modified Mannose/Altrose Derivatives

A highly efficient method to get 3-and 6-modified mannose/ahrose derivatives for oligosaccharide synthesis is described. All the compounds were obtained exclusively with the combinations of 3-and/or 6-hydroxyl protection and/or activation（ including the configuration conversion of 3-carbon atom）.

An embedded vertical‐federated feature selection algorithm based on particle swarm optimisation

In real life,a large amount of data describing the same learning task may be stored in different institutions(called participants),and these data cannot be shared among par-ticipants due to privacy protection.The case that different attributes/features of the same instance are stored in different institutions is called vertically distributed data.The pur-pose of vertical‐federated feature selection(FS)is to reduce the feature dimension of vertical distributed data jointly without sharing local original data so that the feature subset obtained has the same or better performance as the original feature set.To solve this problem,in the paper,an embedded vertical‐federated FS algorithm based on particle swarm optimisation(PSO‐EVFFS)is proposed by incorporating evolutionary FS into the SecureBoost framework for the first time.By optimising both hyper‐parameters of the XGBoost model and feature subsets,PSO‐EVFFS can obtain a feature subset,which makes the XGBoost model more accurate.At the same time,since different participants only share insensitive parameters such as model loss function,PSO‐EVFFS can effec-tively ensure the privacy of participants'data.Moreover,an ensemble ranking strategy of feature importance based on the XGBoost tree model is developed to effectively remove irrelevant features on each participant.Finally,the proposed algorithm is applied to 10 test datasets and compared with three typical vertical‐federated learning frameworks and two variants of the proposed algorithm with different initialisation strategies.Experi-mental results show that the proposed algorithm can significantly improve the classifi-cation performance of selected feature subsets while fully protecting the data privacy of all participants.

The geological heritages in Xinjiang, China： Its features and protection

The geological heritage protection and its development at home and abroad reflect the progress in the aspect from the single protection to the coordination between protection and exploitation. The geopark established by UNESCO has closely combined the protection of geological heritages with the promotion of sustainable development of local economy, which has become the best way to protect geological heritages. The geological heritages in Xinjiang, China, are characterized by their large quantity, rich variety and high grade. The complicated geologic-geomorphic environment in Xinjiang contributes to the creation of various geological heritage types and their spatial distribution, and at the same time makes them under control. The main types of the geological heritages in Xinjiang are the geologic-geomorphic landscapes and the water landscapes. The spatial distribution can be divided into five geological heritage districts： Altay, Junggar, Tianshan, Tarim and Kunlun-Altun, among which Tianshan and Kunlun-Altun are most important. Based on the first systematic investigation of the geological heritages in Xinjiang, it is confirmed that the insufficient coordination between protection and exploitation is the primary cause for the backward situation in the geological heritage protection. To solve the problem, this paper proposes 6 major protection steps--from determining the protection types, the protection forms, the protection modes, the protection grades, the protection sequences to determining the＇ protection zones, brings forward the idea of optimal-selection which integrates geoparks with geological heritage protection areas and other protection areas as protection and exploitation reserve list for 209 well as 5 corresponding modes, and makes a important geological heritages in Xinjiang.

Corrosion Protection with Ion Barrier Coatings(I) Barrier to Anion Coating

A cation exchange membrane is permeable to cation and impermeable to anions. When the membrane is directly coated on a metal surface, the coating will not provide corrosion protection to the substrate, and it delaminated in salt fog testing. When the barrier to anion membrane coated on the surface of a primer, it inhibits anions to diffuse from the environment through the coating.

Fault Current Limiters and Impacts on the System Protection Behavior

A deregulated power market is making short-circuit currents likely to exceed the thermal or mechanical permissible limits of switchgear. Consequently fault current limiters （FCL） become more necessary in power systems. The use of FCLs has an impact on the protection schemes and functions in power systems. Thus, before FCLs can be applied in the network, the impacts on existing protection system must be understood. Depending on the current limiting technique used, today＇s protection concepts may have to be adapted or revised to ensure proper network protection selectivity. A relationship between fault current limiters and protection schemes should be established by taking into account both protection and network specific issues, such as the impact of different FCL technologies, existing and new protection concepts, selectivity and innovative network. This paper is presenting a frame work for accomplishing this task.

Special Problems in Pilot Protections of Transmission Lines Connecting Wind Farms

As the penetration of wind power into power grids increases,higher demands are made on the stability and protection of power systems.Unlike traditional synchronous generators,wind turbines are different on the short-circuit and impedance characteristics,thus some problems exist in pilot protections of transmission lines connecting wind farms.When a single-phase fault occurs on a transmission line,the phase selector based on current-difference sudden-change may mistake the single-phase fault for a phase-to-phase fault.This paper studies the impedance characteristics of an asynchronous wind generator,and analyzes the behavior of a fault phase selector under different conditions.Then,the paper explains that inequality between positive and negative branch coefficients is the cause for the mal-operation of the fault phase selector.Finally,some simulations are done in MATLAB to validate analysis results.

纵向监管、地方选择性供地与产业结构绿色转型

地方政府的有效执行是中国式分权下提升中央发展战略实施绩效的关键,而中央政府对地方政府治理行为的有效监督则是其中至关重要的环节。本文基于2007—2019年中国微观工业用地供应以及中央环保督察的通报结果数据,采用广义双重差分法实证考察中央环保督察对地方政府选择性供地策略的影响及其机制。研究表明,中央环保督察促使地方政府降低污染密集型工业用地供应,增加清洁型工业用地供应,从而推动地区产业结构的绿色转型。机制分析发现,中央环保督察通过地方领导人职业发展激励机制以及产业转型能力机制对不同特征的城市工业土地供应结构策略产生异质性影响。本文结论对高质量发展背景下的央地互动关系与地方利用土地偏向性供给推动产业结构绿色转型具有政策启示。

黄河流域特色保护类村庄种群分布动态与生境选择

基于种群生态学理论,将黄河流域971个国家级特色保护类村庄视作一个地理种群。综合GIS空间分析和数理统计方法,明晰这一种群的分布动态和生境选择特征,进而应用地理探测器对比揭示影响该种群分布的生态因子。结果表明:(1)黄河流域特色保护类村庄的分布密度随种群增长而大幅提高,分布模式随尺度变大由显著聚集转为不显著离散,分布格局随时间推移由多中心向单中心演化,但集聚中心一直位于河谷盆地;(2)黄河流域特色保护类村庄具有“聚中低山区、向阳坡斜坡、喜温暖湿润、近黄河干流”的偏好,趋向中等人口密度、经济欠发达地区分布,主要位于国道沿线30 km、省道沿线10 km、中心城市周边20~80 km、历史城镇周边60 km、旅游景区和文保单位周边20 km范围内;(3)影响特色保护类村庄种群分布的生态因子具有综合性、非等价性和限定性,在黄河流域以文保单位和海拔为主导。

基于LabVIEW的继电保护测试仪自动检测系统设计

针对现有的继电保护测试仪检测程序繁琐,自动化程度低,点对点调试效率低下等问题,提出基于LabVIEW的继电保护测试仪自动检测系统;系统硬件以STM32F407ZGT6为主控芯片研制了继电保护测试仪输出转换装置,自动控制测试仪与标准器连成试验回路,代替人工拆接线行为;系统软件以LabVIEW为开发平台,设计了仪器通信、自动检测、生成报告、强制选择测点等模块,实现了继电保护测试仪的全自动检测;通过对某型继电保护测试仪进行测试,结果表明该系统工作状态稳定,能够实现一键式全自动检测,用户界面友好,可以替代繁琐的人工检测方法,具有一定的工程应用前景。

基于动作时限曲线拟合思想的含DG型配电网后备保护新方法

分布式电源(distributed generation,DG)的接入可能会改变电力系统的短路电流特性,从而影响到反时限过流保护的选择性和配合关系。为此,提出一种基于动作时限曲线拟合思想的含DG型配电网后备保护新方法。从配电网主保护和后备保护的动作时间差入手,建立曲线拟合模型,并运用梯度下降法对反时限过流保护的动作时限曲线进行优化。通过在优化整定计算中考虑保护的选择性以及DG接入所带来的影响,使优化参数适用于配电网中各分段位置。然后,在配电网中设置两相、三相短路故障,并根据故障位置、类型进行整定参数再调整,最终得到参数的全局最优解。基于PSCAD/EMTDC仿真软件进行仿真分析,验证了所提方法的有效性和实用性。

民事在线诉讼当事人权益保障的检视与路径探索

当事人权益保障体现了以当事人为中心的诉讼理念,确保了实现诉讼效益与程序公正的统一。在线诉讼中单一的程序选择权类型难以满足双方当事人需求、证人远程作证提高了质证难度、异步审理稀释了直接言词原则、个人信息保护与庭审公开之间存在张力。应当根据被选择事项性质合理确定选择权类型、加强证人远程作证技术保障、肯定异步审理的价值并明确边界、具体衡量审判公开与个人信息保护价值利益。

能量选择电磁防护方法理论与应用

随着信息技术的发展,电磁空间逐渐成为国家重大公共基础设施及信息网络的基石。近年来,强电磁源生成技术加速发展,对电磁空间安全形成严重威胁,开展强电磁防护技术研究具有迫切的现实需求。能量选择防护方法是一种新型自适应强电磁防护方法,突破了电子设备前门防护“能防”和“兼容”的难题,为强电磁防护提供了新的技术思路。本文对能量选择防护方法进行了系统综述,首先详细介绍了能量选择防护的内涵和相关概念;其次以能量选择表面为例阐述了其工作原理与实现方式,在此基础上对能量选择防护方法的研究现状和最新成果进行介绍,梳理了能量选择防护方法的发展脉络;最后,对能量选择防护方法的未来研究方向进行了展望。

武汉协和医院质子医学中心结构设计及关键问题

武汉协和医院质子医学中心的建设对结构专业设计提出了特殊要求。详细介绍了项目概况、设计条件,阐述了基础与地下室以及上部结构的设计要点,包括桩基选型、结构分缝、结构方案、大跨等特殊部位处理方法等。针对质子区结构设计,重点论述了沉降控制、振动控制、辐射防护、吊装孔设计等关键问题。设计时通过合理设置结构缝、优化桩基础设计、采用大厚度墙板和重密度混凝土、设计特殊预制梁等措施,较好地解决了上述问题;并运用环境调研、数值模拟、现场实测等方法,取得了良好的效果;结构设计能够满足质子医院的建筑功能需求和特殊工艺要求。