Decelerating open-channel flow is a type of flow that gradually moves forward with decreasing velocity and increasing water depth.Although all flow parameters change along the streamwise direction,previous studies hav...Decelerating open-channel flow is a type of flow that gradually moves forward with decreasing velocity and increasing water depth.Although all flow parameters change along the streamwise direction,previous studies have revealed that these parameters’vertical distributions at different sections can be universally described with a single profile when being nondimensionalised by appropriate scales.This study focuses on the population trends of spanwise rotational motions at various sections along the main flow direction by particle imaging velocimetry(PIV)measurement.The wall-normal population distributions of density,radius,swirling strength,and convection velocity of the prograde and retrograde motions show similar trends in uniform open-channel flows.The dimensionless representation is invariant along the main flow direction.This study’s results indicate the self-similar characteristic of population trends of spanwise rotational motions prevails in decelerating open-channel flow.展开更多
Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous R...Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.展开更多
We used sequences of mitochondrial control region (807bp) in 75 samples from three breeding colonies and one wintering population to investigate the genetic diversity and population structure of Marsh Grassbird (Lo...We used sequences of mitochondrial control region (807bp) in 75 samples from three breeding colonies and one wintering population to investigate the genetic diversity and population structure of Marsh Grassbird (Locustella pryeri sinensis) in different regions of China. Marsh Grassbird retained a moderate amount of haplotype (0.759 ± 0.056) and nucleotide diversity (0.002). The results of FST among 3 phy-logeographic units and ФST between breeding and wintering sites revealed little evidence of genetic distinction between different colonies. Neither UPGMA tree structure analysis nor Network picture analysis showed obvious divergence between populations at different locations. Analysis of molecular variance also showed a lack of regional subdivision within Locustella pryeri sinesis, 98.5% of source of variation within populations and only 1.5% among populations. The neutrality test showed negative Fu’s FS value, which, in combination with detection of the mismatch distribution, suggested that population expansion occurred in the evolu-tionary history of this species. This hypothesis was further supported by Tajima’s D test and Fu’s test (D = -1.80, p = 0.02; Fs = -22.11, p = 0.001), this expansion was estimated to occur about 28,700 years ago.展开更多
The dispersed phase in multiphase flows can be modeled by the population balance model(PBM). A typical population balance equation(PBE) contains terms for spatial transport, loss/growth and breakage/coalescence source...The dispersed phase in multiphase flows can be modeled by the population balance model(PBM). A typical population balance equation(PBE) contains terms for spatial transport, loss/growth and breakage/coalescence source terms. The equation is therefore quite complex and difficult to solve analytically or numerically. The quadrature-based moment methods(QBMMs) are a class of methods that solve the PBE by converting the transport equation of the number density function(NDF) into moment transport equations. The unknown source terms are closed by numerical quadrature. Over the years, many QBMMs have been developed for different problems, such as the quadrature method of moments(QMOM), direct quadrature method of moments(DQMOM),extended quadrature method of moments(EQMOM), conditional quadrature method of moments(CQMOM),extended conditional quadrature method of moments(ECQMOM) and hyperbolic quadrature method of moments(Hy QMOM). In this paper, we present a comprehensive algorithm review of these QBMMs. The mathematical equations for spatially homogeneous systems with first-order point processes and second-order point processes are derived in detail. The algorithms are further extended to the inhomogeneous system for multiphase flows, in which the computational fluid dynamics(CFD) can be coupled with the PBE. The physical limitations and the challenging numerical problems of these QBMMs are discussed. Possible solutions are also summarized.展开更多
The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinatio...The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinations generated 193 fragments.The He values varied from 0.322 to 0.463 and the percentage of polymorphic loci ranged from 74.1% to 98.4%,which indicates a high level of genetic diversity.Cluster analysis by Nei's pairwise distance grouped all specimens by geographical origins.AMOVA consistently showed that genetic variation among populations was 8.71%,and most of the variation came from the genetic variation within populations(91.29%).Genetic differentiation among the six populations was moderate;pairwise FST ranged from 0.0282 to 0.1480,which indicated that S.constricta populations along the coast of China are genetically connected.Among all the six populations,the Beihai population is the mostly differentiated from the others,suggesting that Hainan Island and Leizhou Peninsula act as barriers to gene flow.All populations abide isolation by distance model as indicated by Mantel test,except for ZS(Zhoushan) and YQ(Yueqing) populations.Information obtained in this study will provide guidelines for conservation and fishery management of this species in the future.展开更多
Aphis glycines (Hemiptera: Aphididae) is considered as a cosmopolitan pest of cultivated soybean, major difficulties in its control measures may be due to its higher genetic diversity; however, the knowledge about ...Aphis glycines (Hemiptera: Aphididae) is considered as a cosmopolitan pest of cultivated soybean, major difficulties in its control measures may be due to its higher genetic diversity; however, the knowledge about population genetic diversity of this species is limited. This study aimed to represent the genetic differentiation among different geographic populations of soybean aphid in Northeast China. In order to investigate and assess the genetic diversity, genetic differentiation, molecular variance, population structure, ecological importance and evolutionary history of A. glycines, we sequenced a fragment of one protein-coding gene, the cytochrome c oxidase I/of mitochondrial DNA gene. The results showed that four haplotypes were defined among CO 11 gene of 180 sequences of soybean aphid in Northeast China including H1 shared by all the populations. Lower haplotype diversity (Hd=0.3590± 0.0420) and nucleotide diversity (Pi=0.0012±0.0002) were observed and high gene flow was detected in every two populations, while most of the variation (80.81%) arose from variability within A. glycines from individuals. Low genetic differentiation and high gene flow (Nm=2.106) indicated a high migration rate between the populations, which might reveal that gene flow in different geographic populations did not affect by geographical distance. The phylogenetic tree and the haplotype network ofA. glycines were obtained based on sequences of CO Ⅱ gene, there were no significant genealogical branches or clusters recognized in NJ tree, and no clear distribution, delineation of haplotypes were demonstrated in the haplotype network according to geographical location. This study rejected the vicariance hypothesis: geographic isolation could be a barrier and it restricted A. glycines gene flow among 10 populations.展开更多
The population genetic structure of the blacklegged tick Ixodes scapularis was analyzed using 853 individual ticks collected from 12 sites along the eastern seaboard of the United States. PCR-based DNA single strand ...The population genetic structure of the blacklegged tick Ixodes scapularis was analyzed using 853 individual ticks collected from 12 sites along the eastern seaboard of the United States. PCR-based DNA single strand conformation polymorphism(SSCP) was used for analysis of one haploid locus, the mitochondrial cytochrome b(Cyt b), and one diploid locus, the internal transcribed spacer 1(ITS1) of nuclear ribosomal RNA. Seven haplotypes in the Cyt b locus and 13 genotypes in the ITS1 locus were identified. Population differentiation and isolation by distance were found. Distribution of haplotype and genotype frequencies across geographic regions suggests that two distinct populations exist along the Eastern Coast of the US. However, genetic variation among individuals within regions was large. This degree of variation suggests frequent gene flow between regions. Moreover, overall genetic diversity among individuals was much higher in ticks from the southern population.展开更多
The Atlantic sand fiddler crab Uca pugilator is an extremely abundant crab found along the eastern coast of the United States. Fiddler crabs have a life cycle with an obligatory planktonic larval phase of 30-90 days,w...The Atlantic sand fiddler crab Uca pugilator is an extremely abundant crab found along the eastern coast of the United States. Fiddler crabs have a life cycle with an obligatory planktonic larval phase of 30-90 days,which might be expected to lead to widespread larval dispersal and consequent genetic homogeneity over considerable distances. However,a large amount of morphological and behavioral variation is found between northern and southern populations along the eastern coast. This study was undertaken to determine the population genetic structure of U.pugilator and to determine whether these differences may have a genetic basis. The population structure of the fiddler crab was analyzed using 472 individuals collected from 12 sites along the eastern coast. PCR-based single stand conformation polymorphism (SSCP) was used to investigate between-site variation in the mitochondrial 16S rRNA gene of these individuals. Analysis of genetic variation indicated frequent gene flow between nearby localities,but much reduced levels between populations separated by larger geographic distances. Thus,despite the potential for high dispersal by planktonic larvae,population differentiation and isolation by distance is evident between northern and southern populations of U.pugilator. A high amount of genetic differentiation (F ST =0.3468) was found between northern and southern regions suggesting that the morphological and behavioral differences between these two regions have a genetic basis and may represent subspecies.展开更多
Data from the seventh national census show that the mobility of rural population in Liaoning is relatively active.Rural revitalization,as a major deployment for development in the new era,will undoubtedly face a varie...Data from the seventh national census show that the mobility of rural population in Liaoning is relatively active.Rural revitalization,as a major deployment for development in the new era,will undoubtedly face a variety of problems in the implementation process.Through the study of the relationship between rural revitalization and population,it is found that the loss of rural population is one of the important reasons that hinder rural economic development and lead to the imbalance of urban and rural economic development.According to the "push and pull theory" by the demographer Bagne,the paper analyzes the causes of rural population loss in Liaoning Province.The rural population backflow and two-way flow of urban and rural population in Liaoning Province is achieved through industrial interaction between urban and rural areas,innovative development of agriculture,steady follow-up of service industry and other measures,thus revitalizing the rural economy.展开更多
The ice exceeding one million years old has significant meaning for verifying and interpreting the middle Pleistocene transition (MPT) and the relationship between greenhouse gas and climate change. The region near ...The ice exceeding one million years old has significant meaning for verifying and interpreting the middle Pleistocene transition (MPT) and the relationship between greenhouse gas and climate change. The region near Dome A in Antarctica satisfies the conditions for obtaining million-years-old ice since it has low temperatures and low accumulation rates. We analyze the corresponding relation between radar wave features and the crystal orientation fabric (COF) types based on the results of multi-polarization plane radio echo sounding (RES). The results show that, even in the summit of the ice sheet, the COF type is not perfect, but is an elongated single-pole COF. Principal-axis-orientation differences of the COF among the different periods exist and reveal that the ice flow orientations are not constant but deviate clockwise with the increasing depth. This may be related to the adjacent basal valley or both height and position changes of the summit during the glacial-interglacial periods.展开更多
基金the National Natural Science Foundation of China(Grant No.51679020)the Science and Technology Research Program of Chongqing Municipal Education Commission(Grant No.KJQN202100731).
文摘Decelerating open-channel flow is a type of flow that gradually moves forward with decreasing velocity and increasing water depth.Although all flow parameters change along the streamwise direction,previous studies have revealed that these parameters’vertical distributions at different sections can be universally described with a single profile when being nondimensionalised by appropriate scales.This study focuses on the population trends of spanwise rotational motions at various sections along the main flow direction by particle imaging velocimetry(PIV)measurement.The wall-normal population distributions of density,radius,swirling strength,and convection velocity of the prograde and retrograde motions show similar trends in uniform open-channel flows.The dimensionless representation is invariant along the main flow direction.This study’s results indicate the self-similar characteristic of population trends of spanwise rotational motions prevails in decelerating open-channel flow.
基金This work was supported by the National Natural Science Foundation (No. 30471196)the Special Program for Doctorial Site of Universities (No. 200404344011).
文摘Population-wide genetic structure was studied using fluorescent-AFLP markers on 85 apricot (Prunus armeniaca L.) cultivars collected from Kuche, Kashi, Hetian in the Tarim Basin, southern Xinjiang Uygur Autonomous Region of China. The purpose of this study was to determine the genetic structure and genotypic diversity among the different eco-geographical populations. Based on the results from this study, 8 pairs of fluorescent-AFLP primers showed clear electrophoregram and high polymorphism amongst the 64 pairs of EcoR Ⅰ/Mse Ⅰ (Mse Ⅰ - a FAM fluorescent marked primer) primers screened. There was a significant polymorphic difference for the same primer pair in different populations and for the same population with different primer pairs. The percentage of polymorphic loci (P) at species level was higher than Kuche, Hetian, Kashi population levels, respectively. The Nei's gene diversity index (H) and Shannon's information index (I) at species level were higher than those of Kuche, Hetian, and Kashi at population level, respectively. H and I of Kuche population were the highest amongst the three populations. Apricot population genetic diversity was found mainly within the population, Genetic differentiation coefficient between populations (GST) was 0.0882. Gene flow Nm between the populations was 5.1689. Population genetic identity was between 0.9772-0.9811 and genetic distance was between 0.0191-0.0232. These results further indicated that the similarity between populations was higher and the genetic distance between populations was smaller. The UPGMA cluster analysis indicates that the geographical populations at Kuche, Kashi, Hetian were relatively independent Mendelian populations. Concurrently, there was also partial gene exchange between the populations. All the evidences indicated that the genetic diversity in Kuche population was the highest, suggesting that it could be a transition population from wild apricot to cultivated apricot. There were abundant genetic diversities in apricot cultivar populations in southern Xinjiang, China, which provide promising germplasm for further breeding and theoretical basis for biodiversity conservation and utilization for apricot population in this area.
基金supported by National Natural Science Foundation of China (No. 130370221 and No. 30770309)
文摘We used sequences of mitochondrial control region (807bp) in 75 samples from three breeding colonies and one wintering population to investigate the genetic diversity and population structure of Marsh Grassbird (Locustella pryeri sinensis) in different regions of China. Marsh Grassbird retained a moderate amount of haplotype (0.759 ± 0.056) and nucleotide diversity (0.002). The results of FST among 3 phy-logeographic units and ФST between breeding and wintering sites revealed little evidence of genetic distinction between different colonies. Neither UPGMA tree structure analysis nor Network picture analysis showed obvious divergence between populations at different locations. Analysis of molecular variance also showed a lack of regional subdivision within Locustella pryeri sinesis, 98.5% of source of variation within populations and only 1.5% among populations. The neutrality test showed negative Fu’s FS value, which, in combination with detection of the mismatch distribution, suggested that population expansion occurred in the evolu-tionary history of this species. This hypothesis was further supported by Tajima’s D test and Fu’s test (D = -1.80, p = 0.02; Fs = -22.11, p = 0.001), this expansion was estimated to occur about 28,700 years ago.
文摘The dispersed phase in multiphase flows can be modeled by the population balance model(PBM). A typical population balance equation(PBE) contains terms for spatial transport, loss/growth and breakage/coalescence source terms. The equation is therefore quite complex and difficult to solve analytically or numerically. The quadrature-based moment methods(QBMMs) are a class of methods that solve the PBE by converting the transport equation of the number density function(NDF) into moment transport equations. The unknown source terms are closed by numerical quadrature. Over the years, many QBMMs have been developed for different problems, such as the quadrature method of moments(QMOM), direct quadrature method of moments(DQMOM),extended quadrature method of moments(EQMOM), conditional quadrature method of moments(CQMOM),extended conditional quadrature method of moments(ECQMOM) and hyperbolic quadrature method of moments(Hy QMOM). In this paper, we present a comprehensive algorithm review of these QBMMs. The mathematical equations for spatially homogeneous systems with first-order point processes and second-order point processes are derived in detail. The algorithms are further extended to the inhomogeneous system for multiphase flows, in which the computational fluid dynamics(CFD) can be coupled with the PBE. The physical limitations and the challenging numerical problems of these QBMMs are discussed. Possible solutions are also summarized.
基金supported by the grants from National High Technology Research and Development Program (2007AA09Z433)the Major State Basic Research Development Program of China (973 Program, 2010CB-126406)Chinese Ministry of Education (707041)
文摘The genetic variation and differentiation of the razor clam Sinonovacula constricta distributed along the coast of China were studied through amplified fragment length polymorphism(AFLP) analysis.Six primer combinations generated 193 fragments.The He values varied from 0.322 to 0.463 and the percentage of polymorphic loci ranged from 74.1% to 98.4%,which indicates a high level of genetic diversity.Cluster analysis by Nei's pairwise distance grouped all specimens by geographical origins.AMOVA consistently showed that genetic variation among populations was 8.71%,and most of the variation came from the genetic variation within populations(91.29%).Genetic differentiation among the six populations was moderate;pairwise FST ranged from 0.0282 to 0.1480,which indicated that S.constricta populations along the coast of China are genetically connected.Among all the six populations,the Beihai population is the mostly differentiated from the others,suggesting that Hainan Island and Leizhou Peninsula act as barriers to gene flow.All populations abide isolation by distance model as indicated by Mantel test,except for ZS(Zhoushan) and YQ(Yueqing) populations.Information obtained in this study will provide guidelines for conservation and fishery management of this species in the future.
基金Supported by the Special funds for Construction of Modern Agricultural Technology System(CARS-04)Public Welfare Industry(Agriculture)Special Fund(201103002)
文摘Aphis glycines (Hemiptera: Aphididae) is considered as a cosmopolitan pest of cultivated soybean, major difficulties in its control measures may be due to its higher genetic diversity; however, the knowledge about population genetic diversity of this species is limited. This study aimed to represent the genetic differentiation among different geographic populations of soybean aphid in Northeast China. In order to investigate and assess the genetic diversity, genetic differentiation, molecular variance, population structure, ecological importance and evolutionary history of A. glycines, we sequenced a fragment of one protein-coding gene, the cytochrome c oxidase I/of mitochondrial DNA gene. The results showed that four haplotypes were defined among CO 11 gene of 180 sequences of soybean aphid in Northeast China including H1 shared by all the populations. Lower haplotype diversity (Hd=0.3590± 0.0420) and nucleotide diversity (Pi=0.0012±0.0002) were observed and high gene flow was detected in every two populations, while most of the variation (80.81%) arose from variability within A. glycines from individuals. Low genetic differentiation and high gene flow (Nm=2.106) indicated a high migration rate between the populations, which might reveal that gene flow in different geographic populations did not affect by geographical distance. The phylogenetic tree and the haplotype network ofA. glycines were obtained based on sequences of CO Ⅱ gene, there were no significant genealogical branches or clusters recognized in NJ tree, and no clear distribution, delineation of haplotypes were demonstrated in the haplotype network according to geographical location. This study rejected the vicariance hypothesis: geographic isolation could be a barrier and it restricted A. glycines gene flow among 10 populations.
文摘The population genetic structure of the blacklegged tick Ixodes scapularis was analyzed using 853 individual ticks collected from 12 sites along the eastern seaboard of the United States. PCR-based DNA single strand conformation polymorphism(SSCP) was used for analysis of one haploid locus, the mitochondrial cytochrome b(Cyt b), and one diploid locus, the internal transcribed spacer 1(ITS1) of nuclear ribosomal RNA. Seven haplotypes in the Cyt b locus and 13 genotypes in the ITS1 locus were identified. Population differentiation and isolation by distance were found. Distribution of haplotype and genotype frequencies across geographic regions suggests that two distinct populations exist along the Eastern Coast of the US. However, genetic variation among individuals within regions was large. This degree of variation suggests frequent gene flow between regions. Moreover, overall genetic diversity among individuals was much higher in ticks from the southern population.
基金supported by grants from Academic Excellence, Allen Paulson College of Science and Technology and Graduate Funds from Jack N. Averitt College of Graduate Studies, Georgia Southern University
文摘The Atlantic sand fiddler crab Uca pugilator is an extremely abundant crab found along the eastern coast of the United States. Fiddler crabs have a life cycle with an obligatory planktonic larval phase of 30-90 days,which might be expected to lead to widespread larval dispersal and consequent genetic homogeneity over considerable distances. However,a large amount of morphological and behavioral variation is found between northern and southern populations along the eastern coast. This study was undertaken to determine the population genetic structure of U.pugilator and to determine whether these differences may have a genetic basis. The population structure of the fiddler crab was analyzed using 472 individuals collected from 12 sites along the eastern coast. PCR-based single stand conformation polymorphism (SSCP) was used to investigate between-site variation in the mitochondrial 16S rRNA gene of these individuals. Analysis of genetic variation indicated frequent gene flow between nearby localities,but much reduced levels between populations separated by larger geographic distances. Thus,despite the potential for high dispersal by planktonic larvae,population differentiation and isolation by distance is evident between northern and southern populations of U.pugilator. A high amount of genetic differentiation (F ST =0.3468) was found between northern and southern regions suggesting that the morphological and behavioral differences between these two regions have a genetic basis and may represent subspecies.
基金Sponsored by Key Project of Liaoning Social Science Planning Fund in 2019(L19AJY007)。
文摘Data from the seventh national census show that the mobility of rural population in Liaoning is relatively active.Rural revitalization,as a major deployment for development in the new era,will undoubtedly face a variety of problems in the implementation process.Through the study of the relationship between rural revitalization and population,it is found that the loss of rural population is one of the important reasons that hinder rural economic development and lead to the imbalance of urban and rural economic development.According to the "push and pull theory" by the demographer Bagne,the paper analyzes the causes of rural population loss in Liaoning Province.The rural population backflow and two-way flow of urban and rural population in Liaoning Province is achieved through industrial interaction between urban and rural areas,innovative development of agriculture,steady follow-up of service industry and other measures,thus revitalizing the rural economy.
基金the National Natural Science Foundation of China(Grant No.40874060)the 863 projection(Grant No.2006AA09Z152)
文摘The ice exceeding one million years old has significant meaning for verifying and interpreting the middle Pleistocene transition (MPT) and the relationship between greenhouse gas and climate change. The region near Dome A in Antarctica satisfies the conditions for obtaining million-years-old ice since it has low temperatures and low accumulation rates. We analyze the corresponding relation between radar wave features and the crystal orientation fabric (COF) types based on the results of multi-polarization plane radio echo sounding (RES). The results show that, even in the summit of the ice sheet, the COF type is not perfect, but is an elongated single-pole COF. Principal-axis-orientation differences of the COF among the different periods exist and reveal that the ice flow orientations are not constant but deviate clockwise with the increasing depth. This may be related to the adjacent basal valley or both height and position changes of the summit during the glacial-interglacial periods.