Phosphodiesterase 9 localization in cytoplasm and nucleus:the gateway to selective targeting in neuroprotection?

The umbrella term"neurodege ne rative disorders"(NDDs) refers to several conditions characterized by a progressive loss of structure and function of cells belonging to the nervous system.Such diseases affect more than 50million people worldwide.Neurodegenerative disorders are characterized by sundry factors and pathophysiological mechanisms that a re challenging to be fully profiled.Many of these rely on cell signaling pathways to preserve homeostasis,involving second messengers such as cyclic adenosine monophosphate (cAMP)and cyclic guanosine 3',5'-monophosphate(cGMP).Their ability to control the duration and amplitude of the signaling cascade is given by the presence of several common and uncommon effectors.Protein kinases A and G (PKA and PKG),phosphodiesterases (PDEs),and scaffold proteins are among them.

How Multi-services Gateways Enable loT for Utilities

Modem intemet of things technologies bear the promise of cheap, ubiquitous and standard solutions for the smart grid. The end-to-end architecture relies upon transverse service enablement and device connectivity platforms, LTE communications solutions and communication gateways bridge the gap between 3GPP standards and legacy communications solutions. This paper will have a specific focus on the multi-services, multi-protocols gateways that allow connection to legacy assets, and how they are a critical component for the digitalization of power grids and cities. We will illustrate how this gateway--called the Smart Grid Node--has enabled Duke Energy （USA） to initiate its grid modernization project in Cincinnati, Ohio. We will also show that the smart grid node is much more than a communication gateway as it can host very diverse decentralized applications and therefore be a key component to enable multi-services smart city nodes in the near future.

DS-CDMA system outer loop power control and improvement for multi-service

When a new user accesses the CDMA system, the load will change drastically, and therefore, the advanced outer loop power control （OLPC） technology has to be adopted to enrich the target signal interference ratio （Silt） and improve the system performance. The existing problems about DS-CDMA outer loop power control for multi-service are introduced and the power control theoretical model is analyzed. System simulation is adopted on how to obtain the theoretical performance and parameter optimization of the power control algorithm. The OLPC algorithm is improved and the performance comparisons between the old algorithm and the improved algorithm are given. The results show good performance of the improved OLPC algorithm and prove the validity of the improved method for multi-service.

SERVICE-AWARE BASED FUZZY ADMISSION CONTROL SCHEME IN MULTI-SERVICE NETWORKS

Multi-service aggregated transmission is the direction of IP network. Providing different Quality of Service (QoS) assurance for different services has become a crucial problem in future network. Admission control is a vital function for multi-service IP network. This paper proposes a novel fuzzy admission control scheme based on coarse granularity service-aware technique. Different service has discriminative sensitivity to the same QoS characteristic parameter in general. The traffic class can be perceived by the service request parameter and the proposed QoS function. And requirements of dif- ferent applications can be met by maintaining the life parameter. From simulation results, the proposed scheme shows a better QoS provisioning than those traditional fuzzy logic based methods under the same admission probability.

Study on Task Assignment of Two-stage Multi-service Capability Port Logistics Service Providers

This paper mainly studies the problem of multi-task assignment of providers in port logistics service supply chain.As a core enterprise,port plays the role of logistics service integrator.With the continuous development of industrial integration,logistics service providers not only provide one kind of logistics service,but also develop into composite suppliers who capable of providing a variety of logistics services.This paper studies the task assignment problem of multi-service capability providers in the port logistics service supply chain.The two-stage logistics service provider task assignment model was built,which is based on the mixed evaluation method(including MOORA and FMEA)and the multi-objective planning method.Eventually,the effectiveness of the model method was verified by combining with an example.

COMBINED ADMISSION CONTROL AND SCHEDULING IN MULTI-SERVICE NETWORKS

Providing the required metrics for different service respectively is a basic characteristic in multi-service networks. The different service can be accessed and forwarded differently to provide the different transmission performance. The state information between admission control and scheduling can be exchanged each other by the defined correlation coefficient to adjust the flow distribution in progress. The priority queue length measured by scheduler implicitly can describe the priority flows load. And the fair rate can describe the non-priority flows load. Different admission decision will be made according to the state of scheduler to assure the time-delay upper threshold for the priority flows under heavy load and the fairness for elastic flows in light load, respectively. The stability condition was conduced and proved. Simulation results show the policy can ensure both the delay for the priority flows and the minimal throughput for non-priority flows.

THE STUDY OF SCP OVERLOAD CONTROL IN MULTI-SERVICE ENVIRONMENT

This paper first gives a SCP abstract model, then SCP’s overload detection and maximum processing capability are discussed quantitatively. Based upon dynamic adjustment, a new two-level SCP overload control algorithm is proposed. Theoretical analysis and simulation prove the algorithm’s effectiveness and fairness.

DESIGN OF MULTI-SERVICE SDH NETWORKS: FORMULATION AND HEURISTICS

Multi-service SDH networks support both packet- and circuit-switched traffic. Optimal design of such a network means to guarantee the circuit connections and configure a logical packet-switched topology with lowest congestion. This letter first formulates the problem as a mixed integer linear programming, which achieves optimal solution but has high computation. Then a heuristic algorithm is proposed to yield near-optimal solution efficiently. Performance of the algorithm is verified by an example.

STUDY ON THE ACCOUNT-SHARING STRATEGY FOR MULTI-SERVICES IN IMS ONLINE CHARGING MECHANISM

A prepaid subscriber is allowed to simultaneously implement multiple services in online charging mechanism of IP Multimedia Subsystem (IMS). It is a noteworthy discussion to effectively distribute the limited account resources among concurrent services. An account-sharing algorithm is proposed for multi-services,which introduces resource reclamation and redistribution processes based on the resource reservation of standard specifications and dynamically adjusts them according to the changes of Quality of Service (QoS). Three performance indexes are investigated in the simulation experiments, which are average number of accommodated sessions, average number of completed ses- sions, and average number of iterations per accommodated session. The results show that in the normal QoS level, the three indexes of the proposed algorithm averagely increase by 18.7%, 5.4%, and 3.1% compared with the Prepaid Credit Distribution (PCD) algorithm, and by 2.1%, 1.0%, and 1.8% compared with the Prepaid Credit Reclaim (PCR) algorithm. In the poor QoS level, the performance advantages are greater, which averagely increase by 29.1%, 7.1%, and 2.8% compared with PCD, and by 9.4%, 4.1%, and 3.6% compared with PCR.

Echo-state-network classification based multi-services awareness in high-speed optical passive networks

With the challenge of great growing of services diversity,service-oriented supporting ability is required by current high-speed passive optical network( PON). Aimed at enhancing the quality of service( Qo S) brought by diversified-services,this study proposes an echo state network( ESN)based multi-service awareness mechanism in 10-Gigabite ethernet passive optical network( 10GEPON). In the proposed approach,distributed architecture is adopted to realize this ESN based multi-service awareness. According to the network architecture of 10G-EPON,where a main ESN is running in OLT and a number of ESN agents works in ONUs. The main-ESN plays the main function of service-awareness from the total view of various kinds of services in 10G-EPON system,by full ESN training. Then,the reservoir information of well-trained ESN in OLT will be broadcasted to all ONUs and those ESN agents working in ONUs are allowed to conduct independent service-awareness function. Thus,resources allocation and transport policy are both determined only in ONUs. Simulation results show that the proposed mechanism is able to better support the ability of multiple services.

Ancient Zhenyuan, Gateway to East Guizhou

This ancient city has a history going back over 2,000 years.It has witnessed the fusion of multiple ethnic cultures including the Miao,Dong,and Han,and hosts a number of cultural heritage sites.THE old town of Zhenyuan is located in Zhenyuan County,Qiandongnan Miao and Dong Autonomous Prefecture in southwest China’s Guizhou Province.Covering an area of just around three square kilometers,it sits on the banks of the Wuyang River and is surrounded by mountains.Zhenyuan has been a county for more than 2,281 years and was the prefectural capital during the Yuan(1271-1368)and Qing(1644-1911)dynasties.It is a multicultural region,with Miao,Dong,and other ethnic minorities making up nearly half of the population.

The Design of Multi-Connector IoT Central Gateway Based on Raspberry Pi and Docker

With the vigorous development of Internet of Things(IoT)technology,the demand for communication and data exchange between different types of IoT devices is increasing day by day.To solve the problems of diversity and complexity of communication protocols between devices,this paper proposes a design scheme of a multi-connector IoT central gateway based on Raspberry Pi and Docker.Through the research and application of related technologies,by integrating multiple communication interfaces and utilizing containerization technology,an efficient,flexible,and scalable IoT central gateway has been realized,which can support the connection and data interaction of multiple communication protocols and provide strong support for the stable operation and development of the IoT system.

Gateway技术构建酵母双杂分析用辣椒cDNA文库

构建cDNA文库是利用酵母双杂交技术开展特定cDNA分离的重要基础工作。利用Gateway技术,在成功提取辣椒总RNA并分离mRNA的基础上,合成了3种读码框的双链cDNA,通过BP反应构建其相应的入门文库,再通过LR反应将cDNA迅速且定向地重组到酵母双杂分析用目的载体pDESTTM22上,构建成高质量目的文库。经检测入门文库和目的文库滴度均达到(4.0～5.0)×106cfu/mL,文库总容量为(2.4～3.0)×107cfu,平均插入片段大小为1250bp,其质量完全满足于进一步利用酵母双杂体系分析目的基因互作蛋白cDNA。

利用Gateway克隆技术大规模克隆拟南芥转录因子

随着越来越多基因组全序列的测定完成,基因组的研究进入了功能研究阶段。功能基因组的研究需要把大量基因连入不同载体,传统的酶切连接构建载体的方法不能满足这种大规模克隆的需要。Gateway技术是一种高效的大规模克隆系统,而且对载体和宿主没有依赖性。本文利用Gateway大规模克隆技术将16个拟南芥转录因子的ORF克隆入植物表达载pPTV和酵母表达载体pYTV中,酵母融合表达实验和West-ern-blot检测证明了该克隆途径的可行性,并且得到的His-Tag融合蛋白,为拟南芥转录因子的大规模蛋白质组学研究奠定了良好的基础,同时基因序列的聚类分析为将来进一步的功能研究提供了有利信息。

基于Gateway技术的低成本植物双分子荧光互补分析系统

创制基于Gateway重组技术的双分子荧光互补(bimolecular fluorescence complementation,BiFC)载体,同时创制低成本低克隆背景的Gateway入门载体。利用入门载体,采用Gateway技术快速将目标基因重组到BiFC目标载体,并采用农杆菌注射侵染烟草的方法观察荧光互作结果。构建的Gateway入门载体,由于带有ccdB基因,非线性化的载体无法在ccdB敏感菌株中生长,因此消除了载体本身的背景。同时由XcmⅠ酶切后产生的T突出,可以很好地与PCR产物结合,连接效率和普通商业化T载体一致。此外,由于载体可由实验室自行制备且采用了TA克隆,创制入门克隆的成本大大降低。借助该研究改造的BiFC目标载体,目的基因能快速地重组到目的载体用于基因互作鉴定。利用本研究构建的Gateway系统,能够以较低成本快速的进行基因克隆和互作研究。

使用与Gateway技术兼容的T载体获得入门克隆

与Gateway技术兼容的农杆菌双元载体系统已开始应用于植物功能基因组的研究 ,但应用这些载体系统的一个瓶颈问题 ,是如何简单、经济和高效地将PCR产物或其他来源的目的DNA片段构建到入门载体上获得入门克隆 .为此 ,将传统的TA克隆技术与Gateway重组克隆技术进行整合 ,构建了与Gateway技术兼容的两种TA克隆载体 ,用于在克隆PCR产物或其他来源的目的DNA片段的同时获得入门克隆 .利用兼容Gateway技术的TA克隆载体有效地解决了上述瓶颈问题 .

Gateway技术构建结缕草低温和干旱诱导cDNA文库及其质量鉴定

为研究结缕草(Zoysia japonica)胁迫响应信号转导途径中的关键基因,揭示结缕草抗逆分子机制,建立结缕草功能基因组学研究基础平台,以经低温、干旱处理的结缕草为材料,采用Gateway技术构建了首个结缕草低温和干旱诱导的标准cDNA文库。文库质量分析表明,未扩增的原始文库滴度1.76×106 pfu.mL-1,库容7.04×106 pfu,插入片段平均大小大于1kb,重组率90%。文库质量优良,可能包含大量新基因,不仅能为结缕草功能基因组分析提供必要资源,也可为后期进行高通量EST测序、发掘新抗逆相关基因、制作基因芯片等研究奠定基础。

单子叶植物RNA干扰和过表达Gateway载体的构建

基因枪和农杆菌介导的遗传转化是目前常用的两种单子叶植物遗传转化方法。载体的发展和改良是提高植物遗传转化效率的重要基础,RNA干扰载体和过表达载体是目前通过遗传转化研究植物基因功能的主要工具。Gateway克隆技术是一种基于lambda噬菌体特异位点重组特性的通用克隆技术,该技术可以将大批目的基因方便、快捷地连接到受体载体上。本文利用Gateway技术结合传统酶切、连接方法,构建了适用于单子叶植物基因枪和农杆菌转化的RNA干扰Gateway载体pAHC-PSK-RNAi、pClean-G185-RNAi和过表达Gateway载体pAHC-PSK-OE和pClean-G185-OE,为利用基因枪和农杆菌介导的遗传转化,在小麦和水稻等单子叶植物中进行规模化基因功能研究奠定了基础。

Gateway~技术构建交链孢菌JH505 cDNA文库

Gateway○R技术构建cDNA文库,利用λ噬菌体的位点特异性重组,避免使用限制性内酶切割cDNA,能够解决常规方法构建cDNA文库的技术缺陷。首次应用Gateway○R技术构建交链孢菌cDNA文库,经检测cDNA入门文库的滴度达到1×107cfumL,文库总容量为9×107cfu,平均插入片段为1510bp。通过LR重组把入门文库转换为表达文库,表达文库的滴度为1.58×106cfu/mL,文库总容量为6.32×106cfu,平均插入片段大小为1680bp。表达文库的构建为进一步克隆植物激活蛋白基因打下了基础。

应用Gateway技术构建水稻OsDAD1基因的RNA干涉载体

近年来,RNA干涉广泛应用于植物基因功能研究。Gateway技术是通过采用多重载体高效构建目标基因及任何DNA片段克隆和表达载体的研究平台,该技术以λ噬菌体的位点特异性重组体系为基础,两端具有重组位点的DNA片段或目标基因可以非常容易地被重组克隆到含同源重组位点的载体上。这种以重组为基础的克隆体系目前已被广泛应用于分子生物学中来分析基因的功能。本文应用Gateway技术体系替代传统载体构建方法,构建了水稻基因OsDAD1的RNA干涉载体,从而证明了Gateway技术的可行性。