Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also obs...Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also observed during differentiation. Methodology DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR. Results The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation. Conclusion As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo.展开更多
Adipose tissue(AT)is recognized as a complex organ involved in major homeostatic body functions,such as food intake,energy balance,immunomodulation,development and growth,and functioning of the reproductive organs.The...Adipose tissue(AT)is recognized as a complex organ involved in major homeostatic body functions,such as food intake,energy balance,immunomodulation,development and growth,and functioning of the reproductive organs.The role of AT in tissue and organ homeostasis,repair and regeneration is increasingly recognized.Different AT compartments(white AT,brown AT and bone marrow AT)and their interrelation with bone metabolism will be presented.AT-derived stem cell populations-adipose-derived mesenchymal stem cells and pluripotentlike stem cells.Multilineage differentiating stress-enduring and dedifferentiated fat cells can be obtained in relatively high quantities compared to other sources.Their role in different strategies of bone and fracture healing tissue engineering and cell therapy will be described.The current use of AT-or AT-derived stem cell populations for fracture healing and bone regenerative strategies will be presented,as well as major challenges in furthering bone regenerative strategies to clinical settings.展开更多
Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be u...Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be used as an alternative source remains largely elusive.Here we develop a three-dimensional culture system(3DCS)including arginine,glycine,aspartate,and a series of factors.Fourteen-day culture of peripheral blood mononuclear cells(PBMNCs)in 3DCS led to 125-and 70-fold increase of the frequency and number of CD34+cells.Further,3DCS-expanded cHSPCs exhibited the similar reconstitution rate com-pared to CD34+HSPCs in bone marrow.Mechanistically,3DCS fabricated an immunomodulatory niche,secreting cytokines as TNF to support cHSPC survival and proliferation.Finally,3DCS could also promote the expansion of cHSPCs in patients who failed in HSPC mobilization.Our 3DCS successfully expands rare cHSPCs,providing an alternative source for the HSPC therapy,particularly for the patients/donors who have failed in HSPC mobilization.展开更多
基金funded by The Peabody Foundation Inc.,the Anthony and Constance Franchi Fund for Pediatric Orthopaedics at the Mass General Hospital for Children, and the National Natural Science Foundation of China (30801304)Foundation for the Author of National Excellent Doctoral Dissertation of PR China (FANEDD 200977)
文摘Aim The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The expression of GFP in DPSCs was also observed during differentiation. Methodology DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic, adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by RT-PCR. Results The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there was no significant decline of GFP expression during DPSCs differentiation. Conclusion As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for tracking DPSCs in vivo.
文摘Adipose tissue(AT)is recognized as a complex organ involved in major homeostatic body functions,such as food intake,energy balance,immunomodulation,development and growth,and functioning of the reproductive organs.The role of AT in tissue and organ homeostasis,repair and regeneration is increasingly recognized.Different AT compartments(white AT,brown AT and bone marrow AT)and their interrelation with bone metabolism will be presented.AT-derived stem cell populations-adipose-derived mesenchymal stem cells and pluripotentlike stem cells.Multilineage differentiating stress-enduring and dedifferentiated fat cells can be obtained in relatively high quantities compared to other sources.Their role in different strategies of bone and fracture healing tissue engineering and cell therapy will be described.The current use of AT-or AT-derived stem cell populations for fracture healing and bone regenerative strategies will be presented,as well as major challenges in furthering bone regenerative strategies to clinical settings.
基金Data and materials availability:Processed and raw data can be downloaded from NCBI GEO(#GSE122682,and#GSE153421).
文摘Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC shortage.Whether circulating HSPCs(cHSPCs)in steady-state blood could be used as an alternative source remains largely elusive.Here we develop a three-dimensional culture system(3DCS)including arginine,glycine,aspartate,and a series of factors.Fourteen-day culture of peripheral blood mononuclear cells(PBMNCs)in 3DCS led to 125-and 70-fold increase of the frequency and number of CD34+cells.Further,3DCS-expanded cHSPCs exhibited the similar reconstitution rate com-pared to CD34+HSPCs in bone marrow.Mechanistically,3DCS fabricated an immunomodulatory niche,secreting cytokines as TNF to support cHSPC survival and proliferation.Finally,3DCS could also promote the expansion of cHSPCs in patients who failed in HSPC mobilization.Our 3DCS successfully expands rare cHSPCs,providing an alternative source for the HSPC therapy,particularly for the patients/donors who have failed in HSPC mobilization.