[Objectives] To establish Qu ECh ERS based extraction method for determining multiple pesticide residues in okra combined with programmed temperature vaporizer-gas chromatography-triple quadrupole mass spectrometry( P...[Objectives] To establish Qu ECh ERS based extraction method for determining multiple pesticide residues in okra combined with programmed temperature vaporizer-gas chromatography-triple quadrupole mass spectrometry( PTV-GC-MS/MS) and ultra high performance liquid chromatography-mass spectrometry( UPLC-MS/MS). [Methods] In this experiment,124 pesticide residues in okra were determined by an optimized Qu ECh ERS method combined with PTV-GC-MS/MS and UPLC-MS/MS. The pesticides in okra were extracted and purified by the optimized Qu ECh ERS pretreatment method and determined by PTV-GC-MS/MS and UPLC-MS/MS. [Results] In this experiment,124 kinds of pesticides had a good linearity with a limit of detection( LOD) of 0. 000 5-0. 008 0 mg/kg; the average recovery rate at three levels was 65. 5%-128. 1% with relative standard deviation of 2. 2%-9. 7%. [Conclusions] This method is simple,rapid,accurate and sensitive,and can provide reliable and valid data support for the determination of multiple pesticide residues in okra by GC-MS/MS and UPLC-MS/MS.展开更多
基金Supported by Science and Technology Planning Project of Guangdong Province(2014A040401008)
文摘[Objectives] To establish Qu ECh ERS based extraction method for determining multiple pesticide residues in okra combined with programmed temperature vaporizer-gas chromatography-triple quadrupole mass spectrometry( PTV-GC-MS/MS) and ultra high performance liquid chromatography-mass spectrometry( UPLC-MS/MS). [Methods] In this experiment,124 pesticide residues in okra were determined by an optimized Qu ECh ERS method combined with PTV-GC-MS/MS and UPLC-MS/MS. The pesticides in okra were extracted and purified by the optimized Qu ECh ERS pretreatment method and determined by PTV-GC-MS/MS and UPLC-MS/MS. [Results] In this experiment,124 kinds of pesticides had a good linearity with a limit of detection( LOD) of 0. 000 5-0. 008 0 mg/kg; the average recovery rate at three levels was 65. 5%-128. 1% with relative standard deviation of 2. 2%-9. 7%. [Conclusions] This method is simple,rapid,accurate and sensitive,and can provide reliable and valid data support for the determination of multiple pesticide residues in okra by GC-MS/MS and UPLC-MS/MS.
