Application of Zinc, Iron and Boron Enhances Productivity and Grain Biofortification of Mungbean

Deficiencies of essential vitamins,iron(Fe),and zinc(Zn)affect over one-half of the world’s population.A significant progress has been made to control micronutrient deficiencies through supplementation,but new approaches are needed,especially to reach the rural poor.Agronomic biofortification of pulses with Zn,Fe,and boron(B)offers a pragmatic solution to combat hidden hunger instead of food fortification and supplementation.Moreover,it also has positive effects on crop production as well.Therefore,we conducted three separate field experiments for two consecutive years to evaluate the impact of soil and foliar application of the aforementioned nutrients on the yield and seed biofortification of mungbean.Soil application of Zn at 0,4.125,8.25,Fe at 0,2.5,5.0 and B at 0,0.55,1.1 kg ha−1 was done in the first,second and third experiment,respectively.Foliar application in these experiments was done at 0.3%Zn,0.2%Fe and 0.1%B respectively one week after flowering initiation.Data revealed that soil-applied Zn,Fe and B at 8.25,5.0 and 1.1 kg ha−1,respectively,enhanced the grain yield of mungbean;however,this increase in yield was statistically similar to that recorded with Zn,Fe and B at 4.125,2.5 and 0.55 kg ha−1,respectively.Foliar application of these nutrients at flower initiation significantly enhanced the Zn contents by 28%and 31%,Fe contents by 80%and 78%,while B contents by 98%and 116%over control during 2019 and 2020,respectively.It was concluded from the results that soil application of Zn,Fe,and B enhanced the yield performance of mungbean;while significant improvements in seed Zn,Fe,and B contents were recorded with foliar application of these nutrients.

Graft-induced Inheritable Variation in Mungbean and Its Application in Mungbean Breeding

Graft can induce inheritable variations in the progenies of the scion plants. Seedling of mungbean ( Vigna radiata (L.) Wilczek) was grafted onto the stem of sweet potato ( Ipomoea batatas (L.) Lam.). The growth of the scion was maintained until the scion produced selfed seeds. We sowed the seeds for several generations under normal conditions. Distinct genetic variations appeared in the progenies. Similar variations did not appear in the generations of the scion sowed normally without graft. The variations seemed to be induced by the graft and they inherited steadily. For understanding the possible mechanism of the phenomenon (graft_induced inheritable variation), we analyzed the cytoplasmic and genomic DNA of the variations. The results showed that there was no restriction fragment length polymorphism (RFLP) in the cytoplasmic DNA between the original scion and the variation. However, significant difference between the scion and variation was recognized by random amplified polymorphic DNA (RAPD) analysis. In addition, there was no evidence that indicated the gene transformation from stock to scion. Our results suggest that the non_specific grafting has a pragmatic potential for plant breeding and crop improvement and, the genetic variation seems not to be caused simply by DNA transformation but most likely the stress induced mutation.

Effect of Inoculation with Arbuscular Mycorrhizal Fungus on Nitrogen and Phosphorus Utilization in Upland Rice-Mungbean Intercropping System

The effect of arbuscular mycorrhiza fungi （AMF） on plant growth and nutrition utilization in upland rice and mungbean intercropping system was studied. A pot experiment was conducted in the greenhouse and AMF colonization rates of rice and mungbean roots, plant nutrient contents, the ability of nitrogen fixation, and nutrient contents changed in the soil were analyzed. The results were obtained as follows： the rates of AMF colonization of rice and mungbean roots were reached to 14.47 and 92.2% in intercopping system, and increased by 4.11 and 11.95% compared with that of in monocropping; the nirtrogen contents of mungbean and rice were increased by 83.72 and 64.83% in shoots, and 53.76 and 41.29% in roots, respectively, while the contents of iron in shoot and root of mungbean were increased by 223.08 and 60.19%, respectively. In the intercropping system with inoculation of AMF, the biomass of mungbean increased by 288.8%. However, the biomass of rice was not significantly changed among all treatments with or without inoculation of AMF recorded. The number and dry weight of nodules were significantly increased either when mungbean was intercropped with rice or inoculated with AMF. When compared with that of monocropping without AMF inoculation, the contents of nitrogen, phosphorus and iron in nodules of intercropping mungbean with inoculation increased by 80.14, 69.54 and 39.62%, respectively. Additionally, intercropping with AMF inoculation significantly increased soil nitrogen content, but reduced soil phosphorus content. We concluded that upland rice-mungbean intercropping system and inoculation with AMF improved the nutrient uptake, the ability of nitrogen fixation and the growth of mungbean.

Evaluation of Humic Acid Application Methods for Yield and Yield Components of Mungbean

A triplicate field experiment laid out in randomized complete block design was conducted to evaluate different humic acid (HA) application methods at Agricultural Research Farm, of KPK Agricultural University, Peshawar. Three methods of HA application: seed priming, foliar spray and soil application were included in the experiment. Humic acid application methods significantly affected pods plant-1, grains pod-1, 1000 grain weights, and grain yield whereas biological yield was not significantly affected by HA application methods. Humic acid application at the rate of 3 kg&middotha-1 resulted in higher number of pods plant-1, thousand grain weights and grain yield, however it was statistically similar to the treatments where HA was soil applied at rate of 1 and 2 kg&middotha-1, seed priming with 0% (water soaked), 1%, 2% HA solution and foliar spray with 0.01%, 0.05% and 0.1% of HA solution. It is concluded that HA application in all the three methods significantly enhances grain yield and yield components of mungbean.

Tagging and Utilization Bruchid Resistance Gene Using PCR Markers in Mungbean

Sixteen mungbean lines were analyzed using 56 random primers. Different DNA bands were detected between Bruchid resistant lines and susceptible lines. According to the cluster results, the 16 lines can be divided into four groups, including brucid resistant wild types, resistant cultivated lines, resistant progenies and a mixed group. BSA method was used to identify DNA markers that related with bruchid resistant gene by using resistant line and susceptible line and their F2 progeny. One codominant marker was identified, which generated a fragment of 1.79 kb in resistant lines and 1.03 kb in susceptible lines. Finally, this codominant marker was considered to be tightly linked with bruchid resistant gene and could be useful in resistant germplasm identification and marker-assisted selection.

Genetic variation for phytic acid content in mungbean(Vigna radiata L. Wilczek)

Mungbean(Vigna radiata L. Wilczek) is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid(PA), an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid(lpa) in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus(IP), and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen's modified method,respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in102 genotypes ranged from 5.74 to 18.98 mg g-1and 5.85 to 20.02 mg g-1in summer 2011 and rabi 2012, respectively. High heritability was found for PA(0.87 and 0.86) and seed size(0.82 and0.83) but low heritability for IP(0.61 and 0.60). A negative correlation was found between PA and seed size(r =-0.183 and-0.267). Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM(6.001 mg g-1) and JL-781(6.179 mg g-1) showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

Hybridization between salt resistant and salt susceptible genotypes of mungbean(Vigna radiata L.Wilczek)and purity testing of the hybrids using SSRs markers

Six cultivated and two wild genotypes of mungbean（Vigna radiata L. Wilczek） possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12%（average 3.08%）. Molecular analysis confirmed the hybrids purity. All F_1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F_2 seeds ranged from 5–8 per cluster. The F_2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.

Efficiency of seed bio-priming technique for healthy mungbean productivity under terminal drought stress

Recently, drought-induced damaging impact in reducing the crop growth and development is drastically ranked at the top under various abiotic stresses. And especially water stress at the reproductive growth stages termed as terminal drought has become a severe threat for mungbean productivity. To mitigate the drought stress condition, "bio-priming" has emerged as a newly agronomic and sustainable technique in improving the mungbean production. A 2-year field study during Kharif season 2017–2018 was conducted to investigate the efficacy of rhizobacteria seed priming in mungbean(AZRI mung-06), at Agronomic Research Area, Department of Agronomy, Bahauddin Zakariya University, Multan, Pakistan. The experiment comprised two factors containing FA(seed treatments, control(dry seeds), hydro-priming, silicon(Si)-priming, and bio-priming(mixture strains of Pseudomonas fluorescens+Rhizobium phaseoli)) and FB(irrigation water-regimes at various growth stages including leaf formation(L), stem elongation(S)+flowering(F)+pod formation(P) containing treatments are normal irrigation(I(L+S+F+P)) and terminal drought stress(I(F+P))). All the treatments were arranged in randomized complete block design under factorial design and were replicated thrice. Results indicated that the exposure of drought stress at flowering and pod formation stages hampered the morpho-physiological growth and yield of mungbean. Nevertheless, seed priming treatments particularly bio-priming were effective in alleviating the detrimental effects of drought stress. Bio-priming significantly increased the yield and yield components(seeds/plant, 1 000-grain weight and harvest index) of mungbean and regulated the activities/levels of antioxidants(superoxide dismutase, catalase, peroxidase, ascorbic acid, and total phenolics) under drought stress. Compared with the control, bio-priming increased the seed yield of mungbean by 8–12% under normal as well as drought stress conditions during both years of study. Bio-priming also improved the nutrient uptake behavior followed by Si-and hydro-priming treatments under terminal drought stress. The study emphasized the effectiveness of bio-priming as dual seed treatment method may be helpful for vigorous germination of mungbean production along with plant tolerance against terminal drought stress. Among the various treatments, plants treated with bio-priming technique compensated the grain yield due to having strong antioxidant defense system and better nutrient uptake behaviour under terminal drought stress. Economic analysis also concluded that bio-priming is the easiest, cost-effective, friendly, and sustainable approach for the maximization of the mungbean production.

Genetic Diversity in Selected Indian Mungbean [<i>Vigna radiata</i>(L.) Wilczek] Cultivars Using RAPD Markers

Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.

Inheritance of Seed Color and Luster in Mungbean(Vigna radiata)

The inheritance of seed color and seed luster of mungbean was studied by using accessions/varieties with different seed color,black (Bkck),green (KPS1) and green (KPS2).In KPS2×Bkck combination,the p2 seed color was shiny black.The F3 data indicated the following genotype:3B-for black seed color,and 1 bb for green seed color.Plant purple petiole gene and black seed color gene were very close linkage.There was no segregation between them.Perhaps the same gene B controlled the color of purple petiole.Moreover in KPS1X Bkck combination F3 seed color popuktion showed a wide rang of phenotypic variability.Perhaps seed luster was controlled by more than two genes.

Heredity Analysis and Gene Mapping of Bruchid Resistance of a MungbeanCultivar V2709

Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Vegetable Center, Asian Vegetable Research and Development Center （AVRDC）. Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus （named as Br2）. Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.

Inheritance of Resistance to Mungbean Yellow Mosaic Virus (MYMV) in Inter and Intra Specific Crosses of Mungbean (<i>Vigna radiata</i>)

The objective of this research was to study the mode of inheritance of resistance to mungbean yellow mosaic virus (MYMV) in inter TNAU RED × VRM (Gg) 1 and intra KMG 189 × VBN (Gg) 2 specific crosses of mungbean. An infector row technique was used for evaluating parents, F1, F2 and F3 plants for MYMV resistance. No insecticide was sprayed in order to maintain the natural whitefly population in experimental field. In the field condition, only after 80% of plants showed MYMV incidence, and the scoring of the test materials was done by MYMV disease rating scale. According to the mean disease score, the mungbean genotypes were categorized into five groups resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) and highly susceptible (HS). 3 (Susceptible): 1 (Resistance) was observed in all two crosses of all F2 population and it showed that the dominance of susceptibility over the resistance and the results of the F3 segregation (1:2:1) confirm the segregation pattern of the F2 segregation. Collectively all the two crosses F2 and F3 generations results suggested that a single recessive gene is involved in resistance against the MYMV disease.

Efficacy of Less Toxic Phytochemicals to Prevent the Insect Vector of <i>Mungbean Yellow Mosaic Virus</i>(<i>MYMV</i>)

Planting material BARI mungbean-5 and two phytochemicals viz. Imitaf, active ingredient imidacloprid and ACmix, active ingredient chloropyriphos (50%) + Cypermethrin (5%) were used in this experiment. In case of Imitaf, the lowest percent disease incidence (10.12% per plant and 3.51% per plot) and severity (7.21%) were recorded in four times spray (T4) at 70 DAS. The highest percent disease incidence (36.50% per plant and 11.37% per plot) and severity (23.33%) were recorded in no spray (T0) at 70 DAS. In case of ACmix, the lowest percent disease incidence (12.21% per plant and 4.21% per plot) and severity (7.53%) were recorded in four times spray (T4) at 70 DAS. The highest percent disease incidence (36.49% per plant and 11.37% per plot) and severity (36.67%) was recorded in T0 at 70 DAS. The highest number of flower, no of pod and yield was found in four times spray (T4) that was higher in Imitaf than ACmix. The highest chlorophyll content was recorded in four times spray (T4) for Imitaf (49.62 μmol·m-2·s-1) and ACmix (56.80 μmol·m-2·s-1), and the lowest chlorophyll content was recorded in T0 for Imitaf (42.01 μmol·m-2·s-1) and ACmix (48.34 μmol·m-2·s-1). However from the results of this experiment it was revealed that Imitaf gave the better performance to control the insect vector (Whitefly) of MYMV with four times spray.

Identification of Leaf Based Physiological Markers for Drought Susceptibility during Early Seedling Development of Mungbean

Drought is a recurrent phenomenon in many of the countries. Moisture stress during seedling stage is very critical in determining the establishment of the crop and its further development and yield. Identifying drought tolerance mechanism and physiological markers of drought susceptibility is this crop during seedling stress would be useful tool in future genetic manipulation programme to establish drought tolerance in this crop. Thus the present study aimed for quickly identifying reliable physiological markers for drought susceptibility through evaluation of physiological and biochemical changes in leaves of two contrasting mungbean (Vigna radiata L. Wilczek) cultivars i.e. K 851 (drought tolerant) and PDM 84-139 (drought susceptible) during seedling development. A range of four external water potentials (i.e. -1.0, -2.0, -3.0 and -4.0 bars), besides glass distilled water as control (0.0 bar), was used. Parameters like leaf area, relative leaf water content, chlorophyll content, chlorophyll stability indices in both the cultivars were decreased with the increasing magnitude of stress. By and large phenols and ascorbic acid content were increased with the stress level but the trend was not consistent. A steady rise in proline, hydrogen peroxide content and lipid peroxidation was found with water stress. Out of two cultivars tested, drought tolerant cultivar K 851 was better in leaf water balance and higher accumulation of phenols, proline and ascorbic acid than PDM 84-139. The correlation study indicated lipid peroxidation and H2O2 content as valuable physiological markers for screening of drought susceptibility.

Effect of Cooking on Amylose Contents of Mungbean （Phaseolus aureus） and Chickpea （Cicer arietinum L.） Varieties

Keeping in view the importance of amylose for various food industries as well as its importance on domestic level, the current study was conducted to analyze the amylose content of pulses of common use e.g. mungbean （NM-92, NM-98 and Ramazan） and kabuli type chickpea （CMNKI0-99, CMNK452-2 and Hassan 2k） varieties and quantifying the influence of cooking （boiling） on the amylose contents of pulses. The data revealed that moisture and arnylose content of uncooked mungbean varieties ranged 9.01-9.47% and 20.06-22.26% respectively. The moisture and amylose content of Kabuli type chickpea varieties varied 8.52-8.79% and 20.25-22.83% respectively. Moisture content of mungbean （64.16-66.08%） as well as chickpea （55.56-61.52%） varieties increased after cooking. For all the three varieties of mungbean the observed cooking time was 11 minutes. The maximum value of amylose content was assayed for NM-98 （20.74%） followed by Ramzan （20.24%）. The cooking time of Kabuli type chickpea varieties CMNK-452-2 and CMNK-10-99 was 60 min. Hassan 2K observed more cooking time （90 min） and highest value of amylose content in cooked （19.01%） samples. The effect of cooking/varietals on sensory evaluation （appearance, flavor, texture and overall acceptability） of mungbean varieties was statistically non significant attributes. Among chickpea varieties, Hassan 2K was ranked lowest due to its small size and hard texture and the sensory attributes were statistically significant （P 〈 0.05） as compared to the other two varieties.

Productivity, quality and soil health as influenced by lime in ricebean cultivars in foothills of northeastern India

To evaluate the response to lime on cultivars of ricebean(Vigna umbellata), a field experiment was conducted during the two consecutive rabi seasons of 2010–2011 and 2011–2012 in the Nagaland foothills, India. The experiment used a split-plot design with four levels of lime(control, 0.2, 0.4and 0.6 t ha-1) in main plots and four ricebean cultivars(RBS-16, RBS-53, PRR-2, and RCRB-4) in sub-plots with three replicates. The results revealed that increasing levels of lime(in the furrow)from 0 to 0.6 t ha-1significantly increased growth, yield attributes and yield. The quality parameters of ricebean were also influenced significantly by the application of lime. Maximum gross return(INR 39,098 ha-1), net return(INR 27,281 ha-1), benefit:cost(B:C) ratio(2.29),production efficiency, and economic efficiency were also realized with the application of lime at 0.6 t ha-1. Among the ricebean cultivars, RBS-53 produced significantly higher growth, yield attributes, grain yield, straw yield, biological yield, and harvest index. Similarly, yield and protein content were higher in RBS-53. Maximum gross return, net return, B:C ratio, production efficiency, and economic efficiency were observed for RBS-53.

一种绿豆柱头外露突变体的转录组分析

柱头外露作为提高作物异交率、制种纯度和降低制种成本的优良性状,在杂交制种中得到了广泛的利用。绿豆是一种闭花授粉的作物,被报道的柱头外露突变体很少。通过对冀绿7号的化学诱变,发现了1个柱头外露突变体se2,为明确该突变体柱头外露的分子机制,对该突变体及其野生型冀绿7号即将开放的花蕾进行了转录组测序(RNA-seq)分析。根据差异倍数|log2(Fold Change)|≥1,P≤0.05的标准筛选,在se2中共得到572个差异表达基因(differentially expressed genes,DEGs),其中262个DEGs上调,310个DEGs下调。在基因本体(gene ontology,GO)数据库中,差异表达基因显著富集到代谢和生物合成等生物过程,定位在质外体和细胞壁、细胞膜等区域,与结合、氧化还原等分子功能有关。在京都基因与基因组百科全书(kyoto encyclopedia of genes and genome,KEGG)数据库中,差异表达基因显著富集在植物激素信号传导、次生代谢物生物合成等通路。功能注释发现许多有关细胞壁合成和代谢、细胞分裂和细胞扩张、植物激素相关的基因,因此推测se2突变体中龙骨瓣的细胞分裂、细胞扩张以及植物激素信号传导过程受到影响,从而导致了柱头外露。本研究为今后探究绿豆柱头外露的分子机制以及该性状在绿豆杂种优势中的利用奠定了基础。

绿豆窄叶突变体vrnl11的精细定位

绿豆叶形突变体和叶形调控基因的鉴定,可为品种叶形的遗传改良提供种质资源,也有利于解析叶片发育的遗传调控机理。从皖科绿3号EMS诱变突变体库中筛选到窄叶突变体vrnl11,利用vrnl11/皖科绿3号和vrnl11/中绿1号的杂交后代进行遗传分析,用χ^(2)检验确定F_(2)群体中不同表型植株的分离模式。以vrnl11和中绿1号及中绿5号构建的2个F_(2)群体为定位群体,利用BSA测序技术和图位克隆的方法完成vrnl11的精细定位。表型鉴定结果表明,vrnl11叶片宽和叶面积较野生型皖科绿3号分别减少25.7%和21.7%。遗传分析表明,vrnl11的窄叶表型受单隐性核基因调控。BSA测序分析将突变位点定位在第11染色体上15.0 Mb至末端4.7 Mb的区间内。利用新开发的多态性分子标记将vrnl11定位在标记nl-61和nl-46之间186.5 kb的区间内,包含9个预测基因。这些研究结果为克隆vrnl11和解析绿豆叶片生长发育的分子调控机理提供理论依据。

绿豆窄叶突变体vrnl9基因的精细定位与转录组分析

叶片是绿豆最重要的光合作用场所,其形态结构影响光合作用、群体结构和产量。筛选和鉴定绿豆叶形突变体材料,为探究叶片发育的分子调控机理和叶形遗传改良奠定基础。在皖科绿3号EMS(Ethyl methyl sulphonate)诱变突变体库中鉴定出1个窄叶突变体vrnl9,并对该突变体进行表型鉴定、基因定位和转录组分析。表型分析发现,窄叶突变体vrnl9叶片宽较野生型皖科绿3号显著减小,叶面积和叶柄长也显著缩减;突变体主茎退化,生育期延长10 d。在突变位点的定位中,本研究利用苏绿16-10与突变体vrnl9杂交构建的F2群体进行遗传分析和基因精细定位。结果表明,窄叶突变体vrnl9的窄叶表型受单个隐性核基因控制(χ^(2)=1.40)。BSA测序分析将突变位点定位在第9染色体上0~3.1 Mb的区间内,结合图位克隆的方法,本研究将窄叶基因vrnl9定位在标记NL-15和NL-28之间354.6 kb的区间内。转录组学分析发现,与野生型皖科绿3号相比,突变体vrnl9中有182个基因的表达量发生显著改变,其中86个上调、96个下调。KEGG分析结果表明,差异表达基因显著富集在植物激素信号传导、萜类骨架的生物合成、玉米素的生物合成等代谢通路。这些研究结果为克隆窄叶基因vrnl9和解析绿豆叶片生长发育的分子调控机理提供理论依据。

Sequence Information on Simple Sequence Repeats and Single Nucleotide Polymorphisms through Transcriptome Analysis of Mungbean

Mungbean （Vigna radiata （L.） Wilczek） is a unique species in its ability to fix atmospheric nitrogen, with early maturity, and relatively good drought resistance. We used 454 sequencing technology for transcriptome sequencing. A total of 150 159 and 142 993 reads produced 5 254 and 6 374 large contigs （〉_500 bp） with an average length of 833 and 853 for Sunhwa and Jangan, respectively. Functional annotation to known sequences yielded 41.34% and 41.74% unigenes for Jangan and Sunhwa. A higher number of simple sequence repeat （SSR） motifs was identified in Jangan （1 630） compared with that of Sunhwa （1 334）. A similar SSR distribution pattern was observed in both varieties. A total of 8 249 single nucleotide polymorphisms （SNPs） and indels with 2 098 high-confidence candidates were identified in the two mungbean varieties. The average distance between individual SNPs was -860 bp. Our report demonstrates the utility of transcriptomic data for implementing a functional annotation and development of genetic markers. We also provide large resource sequence data for mungbean improvement programs.