Direct observation was made by using the patch-clamp technique with a specially designed microperfusion system to investigate the effect of acetylcholine (Ach 10^(-6) mol/L) elicited endothelium-derived relaxing facto...Direct observation was made by using the patch-clamp technique with a specially designed microperfusion system to investigate the effect of acetylcholine (Ach 10^(-6) mol/L) elicited endothelium-derived relaxing factor (EDRF) on the calcium-activated potassium channel (IK(Ca))in the smooth muscle cells of mesenteric resistance vessels in Wistar rats. Activation of IK(Ca) was firstly observed by inducing the elicited EDRF or sodium nitroprusside (SNP 10^(-8) mol/L) under various clamping voltages in cell-attached configuration. While the pipette solution contained KCl 126 mmol/L and the bath solution contained KCl 5.9 mmol/L, two types of conductances of calcium-activated potassium current being 76.4±2.3 pS(mean±S.E. n = 7) and 160.3±7.5 pS (mean±S.E. n= 7) were recorded during the EDRF activation, one type of conductance being 100.5±2.8 pS (mean±S.E. n = 6) was activated by nitric oxide (NO) which is an effective component from SNP. Differences in kinetic characteristics of these channels between EDRF and NO activation were found, particularly the probability of the channel being open in EDRF activation was obviously greater than that in NO stimulation. It has been shown that the potassium channel mechanisms involved in the EDRF and NO actions might be different.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Direct observation was made by using the patch-clamp technique with a specially designed microperfusion system to investigate the effect of acetylcholine (Ach 10^(-6) mol/L) elicited endothelium-derived relaxing factor (EDRF) on the calcium-activated potassium channel (IK(Ca))in the smooth muscle cells of mesenteric resistance vessels in Wistar rats. Activation of IK(Ca) was firstly observed by inducing the elicited EDRF or sodium nitroprusside (SNP 10^(-8) mol/L) under various clamping voltages in cell-attached configuration. While the pipette solution contained KCl 126 mmol/L and the bath solution contained KCl 5.9 mmol/L, two types of conductances of calcium-activated potassium current being 76.4±2.3 pS(mean±S.E. n = 7) and 160.3±7.5 pS (mean±S.E. n= 7) were recorded during the EDRF activation, one type of conductance being 100.5±2.8 pS (mean±S.E. n = 6) was activated by nitric oxide (NO) which is an effective component from SNP. Differences in kinetic characteristics of these channels between EDRF and NO activation were found, particularly the probability of the channel being open in EDRF activation was obviously greater than that in NO stimulation. It has been shown that the potassium channel mechanisms involved in the EDRF and NO actions might be different.
文摘目的:观察松解理筋手法联合微波理疗治疗非特异性下腰痛(寒湿瘀阻证)的临床疗效及对血液流变学的影响。方法:将124例患者随机分为对照组和观察组各62例。对照组采用理疗+牵引方法治疗;观察组采用松解理筋手法+微波理治疗。两组均以6 d为1个疗程,连续治疗3个疗程。统计两组患者治疗前后日本骨科学会腰痛疗效评定量表(Japanese orthopaedic association scores,JOA)积分、疼痛程度、身体损害指数评定量表(PⅡ)和寒湿瘀阻证评分,并检测治疗前后血液流变学指标。结果:观察组JOA评分改善优良率为72.59%,对照组为54.84%,两组比较差异有统计学意义(χ2=4.226,P<0.05);观察组JOA量表主观症状、运动障碍、日常功能活动受限评分和总分均显著低于对照组(P<0.01);观察组腰前屈、腰后伸、腰侧屈、单腿直腿抬高、脊柱触痛、双侧主动直腿抬高、仰卧起坐评分及总分均显著低于对照组(P<0.01);观察组疼痛程度评分和寒湿瘀阻证评分均显著低于对照组(P<0.01);观察组全血黏度、血浆黏度、纤维蛋白原和血小板聚集率均显著低于对照组(P<0.01)。结论:松解理筋手法联合微波理疗治疗寒湿瘀阻型非特异性下腰痛,能促进患者血液循环,减轻疼痛,促进功能的恢复,从而提高患者的日常生活能力。
