Objective：To investigate the production of nitric oxide（NO） and the expression of inducible nitric oxide synthase （iNOS）, and their possible role in abdominal aortic aneurysm （AAA）. Methods： A total of 28 pati...Objective：To investigate the production of nitric oxide（NO） and the expression of inducible nitric oxide synthase （iNOS）, and their possible role in abdominal aortic aneurysm （AAA）. Methods： A total of 28 patients with AAA, 10 healthy controls, and 8 patients with arterial occlusive disease were enrolled into this study. Standard colorimetric assay was used to examine NO concentration in plasma from patients with AAA and normal controls, and in cultured smooth muscle cells （SMCs）. Expression of iNOS in aortas and cultured SMCs were detected by immunochemistry. The correlation of iNOS expression with age of the patient, size of aneurysm, and degree of inflammation was also investigated by Cochran-Mantel-Haenszel χ^2 test and Kendall correlation. Results ： Expression of iNOS increased significantly in the wall of aneurism in the patients with AAA compared to the healthy controls （P〈0.05） and the patients with occlusive arteries （P〈0.05）. iNOS protein and media NOx （nitrite＋nitrate） also increased in cultured SMCs from human AAA （n=4, P〈0.05）, while plasma NOx decreased in patients with AAA （n= 25） compared to the healthy controls （n=20）. There was a positive correlation between iNOS protein and the degree of inflammation in aneurismal wall （Kendall coefficient = 0. 5032, P= 0. 0029）. Conclusion： SMCs and inflammatory cells are main cellular sources of increased iNOS in AAA, and NO may play a part in pathogenesis in AAA through inflammation, SMCs and oxidative stress.展开更多
The homocysteine （Hcy）-induced tissue factor （TF） expression in human vascular smooth muscle cells （VSMCs） and the effect of Hcy on the activity of nuclear factor-kappaB （NF-кB） and the expression of inducibl...The homocysteine （Hcy）-induced tissue factor （TF） expression in human vascular smooth muscle cells （VSMCs） and the effect of Hcy on the activity of nuclear factor-kappaB （NF-кB） and the expression of inducible nitric oxide synthase （iNOS） were investigated. Human umbilical artery VSMCs were cultured by tissue explanting method, identified by α-actin immunohistochemistry, and incubated with different concentrations of Hcy/PTDC （NF-кB inhibitor）. Semi-quantitative RT-PCR was performed to detect the expression of TF mRNA in VSMCs. Flow cytometry was used to assay the expression of TF protein on the surface of VSMCs and the expression of iNOS in VSMCs. Western blot was carried out to detect the expression of NF-кB protein in nuclei. The results showed that Hcy could induce VSMCs expressing TF mRNA significantly after the VSMCs were incubated with Hcy at concentrations of 10, 100, 500 μmol/L respectively. There was low expression level of TF protein on the surface of the resting VSMCs and Hcy could also induce VSMCs expressing TF pro- tein on the cell surface in different concentrations. Additionally, Hcy could rapidly induce the activation of NF-кB and this effect could be significantly inhibited by PDTC. Hcy alone could not induce the expression of iNOS in VSMCs. It was concluded that Hcy could significantly induce the expression of TF in VSMCs and enhance the activation of NF-ΚB, subsequently mediate TF gene expression and protein synthesis. NF-кB-mediated expression of TF in VSMCs might be the important mechanism of atherosclerosis and thrombosis induced by Hcy.展开更多
Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebr...Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebrae (L3) transverse process syndrome model rat and to observe the effect of needle-knife therapy. Methods: One hundred and sixty male SD rats were randomly divided into normal group, model group, aminoguanidine (AG) group, needle-knife group, 40 rats in each group. The L3 transverse process syndrome rat model was established, and after treatment of needle-knife and AG, iNOS activities and NO contents and histomorpholocal changes in the soft tissues around L3 transverse process on 1, 3, 7 and 14 days were observed in the groups. Results: Compared with the normal group, iNOS activity and NO content in the model group were significantly increased (P<0.01); Compared with the model group, iNOS activities and NO contents were significantly decreased in both the needle-knife group and the AG group (both P<0.01); And both iNOS activities and NO contents were identical with both local inflammation response and injured degrees of the injured tissue in the groups. Conclusion: Needle-knife therapy can significantly inhibit generation of NO, alleviate inflammatory response and injured degree of the injured soft tissue, improve microcirculation, prevent formation of pathological scar tissue, and promote repair of the chronic soft tissue injury.展开更多
Objective: To investigate the effect of LPS on the proliferation and viability of rat vascular smoothmuscle cell in vitro. Methods: In cultured vascular smooth muscle cells(VSMCs) of SD rat, it was found that 1× ...Objective: To investigate the effect of LPS on the proliferation and viability of rat vascular smoothmuscle cell in vitro. Methods: In cultured vascular smooth muscle cells(VSMCs) of SD rat, it was found that 1× 10 -1~1 × 10-5 g/ml LPS markedly stimulated the proliferation and DNA synthesis of VSMC in a dose--dependentmanner (P CO. 05). 5 × 10- 4-- 10 -3 g/ml LPS markedly inhibited the proliferation, DNA synthesis and viabilityof VSMC in a time--dependent manner (P <0. 01). N (--Nitro--L--Arginine (L--NNA )which could decrease theproduction of NO by inhibiting nitric oxide synthase (NOS) and antagonize the inhibitory effect of LPS. Thecontents of NO3, NO2 in medium were markedly increased in LPS group (P <0. 01), 48 h group vs 24 h groupincreased by 9. 1%, 72 h group vs 48 h group increased by 4. 5%. In high dose LPS group, positiveimmunohistochemical staining for inducible nitric oxide synthase was observed. Results:It was demonstrated thatlow dose LPS stimulated the proliferation and DNA synthesis of VSMC, while LPS at high dose markedly inhibitedthe proliferation, DNA synthesis and viability of VSMC. Conclusion: The inhibition of VSMC proliferationinduced by LPS is probably due to the increased release of NO by VSMC.展开更多
目的:研究按摩对大鼠骨骼肌急性损伤修复过程中肌卫星细胞激活关键因子神经型一氧化氮合成酶(n NOS)、肝细胞生长因子(HGF)m RNA表达的影响,探讨按摩对于骨骼肌急性损伤修复的作用。方法:将44只SPF级成年雄性SD大鼠按随机抽样法分为3组...目的:研究按摩对大鼠骨骼肌急性损伤修复过程中肌卫星细胞激活关键因子神经型一氧化氮合成酶(n NOS)、肝细胞生长因子(HGF)m RNA表达的影响,探讨按摩对于骨骼肌急性损伤修复的作用。方法:将44只SPF级成年雄性SD大鼠按随机抽样法分为3组,正常对照组(A组,n=4),自然恢复组(C组,n=24),按摩组(M组,n=16)。A组不做任何处理,C、M组用改良打击器制备大鼠腓肠肌急性损伤模型。C组不给予按摩,M组于造模后第3天介入按摩治疗。C、M组于造模后第7天、第14天、第21天及第28天取实验动物腓肠肌样本,HE染色观察组织病理改变,实时荧光PCR检测肌卫星细胞中n NOS、HGF m RNA表达量。结果:按摩干预治疗后,M、C组与A组比较,各时间点n NOS、HGF m RNA表达量均高于A组,且具有显著差异(P<0.01);M组与C组比较,在7天、第14天、第21天及第28天n NOS、HGF m RNA表达量M组均高于C组(P<0.01)。M组与C组比较,损伤区域成肌细胞更多,肌丝稠密,肌卫星细胞增殖明显,血供更丰富,坏死组织恢复更快。结论:按摩可有效提高肌卫星细胞中n NOS、HGF m RNA表达水平,激活更多的肌卫星细胞,促进受损骨骼肌的修复再生。展开更多
基金Supported by the National Natural Science Foundation of China (No. 39800177)
文摘Objective：To investigate the production of nitric oxide（NO） and the expression of inducible nitric oxide synthase （iNOS）, and their possible role in abdominal aortic aneurysm （AAA）. Methods： A total of 28 patients with AAA, 10 healthy controls, and 8 patients with arterial occlusive disease were enrolled into this study. Standard colorimetric assay was used to examine NO concentration in plasma from patients with AAA and normal controls, and in cultured smooth muscle cells （SMCs）. Expression of iNOS in aortas and cultured SMCs were detected by immunochemistry. The correlation of iNOS expression with age of the patient, size of aneurysm, and degree of inflammation was also investigated by Cochran-Mantel-Haenszel χ^2 test and Kendall correlation. Results ： Expression of iNOS increased significantly in the wall of aneurism in the patients with AAA compared to the healthy controls （P〈0.05） and the patients with occlusive arteries （P〈0.05）. iNOS protein and media NOx （nitrite＋nitrate） also increased in cultured SMCs from human AAA （n=4, P〈0.05）, while plasma NOx decreased in patients with AAA （n= 25） compared to the healthy controls （n=20）. There was a positive correlation between iNOS protein and the degree of inflammation in aneurismal wall （Kendall coefficient = 0. 5032, P= 0. 0029）. Conclusion： SMCs and inflammatory cells are main cellular sources of increased iNOS in AAA, and NO may play a part in pathogenesis in AAA through inflammation, SMCs and oxidative stress.
文摘The homocysteine （Hcy）-induced tissue factor （TF） expression in human vascular smooth muscle cells （VSMCs） and the effect of Hcy on the activity of nuclear factor-kappaB （NF-кB） and the expression of inducible nitric oxide synthase （iNOS） were investigated. Human umbilical artery VSMCs were cultured by tissue explanting method, identified by α-actin immunohistochemistry, and incubated with different concentrations of Hcy/PTDC （NF-кB inhibitor）. Semi-quantitative RT-PCR was performed to detect the expression of TF mRNA in VSMCs. Flow cytometry was used to assay the expression of TF protein on the surface of VSMCs and the expression of iNOS in VSMCs. Western blot was carried out to detect the expression of NF-кB protein in nuclei. The results showed that Hcy could induce VSMCs expressing TF mRNA significantly after the VSMCs were incubated with Hcy at concentrations of 10, 100, 500 μmol/L respectively. There was low expression level of TF protein on the surface of the resting VSMCs and Hcy could also induce VSMCs expressing TF pro- tein on the cell surface in different concentrations. Additionally, Hcy could rapidly induce the activation of NF-кB and this effect could be significantly inhibited by PDTC. Hcy alone could not induce the expression of iNOS in VSMCs. It was concluded that Hcy could significantly induce the expression of TF in VSMCs and enhance the activation of NF-ΚB, subsequently mediate TF gene expression and protein synthesis. NF-кB-mediated expression of TF in VSMCs might be the important mechanism of atherosclerosis and thrombosis induced by Hcy.
基金supported by a grant from National "973" Project (No: 2006CB504508)
文摘Objective: To study on relationship of inducible nitric oxide synthase (iNOS) activity and nitric oxide (NO) content in the injured local soft tissue with injured degrees of the soft tissue in the third lumbar vertebrae (L3) transverse process syndrome model rat and to observe the effect of needle-knife therapy. Methods: One hundred and sixty male SD rats were randomly divided into normal group, model group, aminoguanidine (AG) group, needle-knife group, 40 rats in each group. The L3 transverse process syndrome rat model was established, and after treatment of needle-knife and AG, iNOS activities and NO contents and histomorpholocal changes in the soft tissues around L3 transverse process on 1, 3, 7 and 14 days were observed in the groups. Results: Compared with the normal group, iNOS activity and NO content in the model group were significantly increased (P<0.01); Compared with the model group, iNOS activities and NO contents were significantly decreased in both the needle-knife group and the AG group (both P<0.01); And both iNOS activities and NO contents were identical with both local inflammation response and injured degrees of the injured tissue in the groups. Conclusion: Needle-knife therapy can significantly inhibit generation of NO, alleviate inflammatory response and injured degree of the injured soft tissue, improve microcirculation, prevent formation of pathological scar tissue, and promote repair of the chronic soft tissue injury.
文摘Objective: To investigate the effect of LPS on the proliferation and viability of rat vascular smoothmuscle cell in vitro. Methods: In cultured vascular smooth muscle cells(VSMCs) of SD rat, it was found that 1× 10 -1~1 × 10-5 g/ml LPS markedly stimulated the proliferation and DNA synthesis of VSMC in a dose--dependentmanner (P CO. 05). 5 × 10- 4-- 10 -3 g/ml LPS markedly inhibited the proliferation, DNA synthesis and viabilityof VSMC in a time--dependent manner (P <0. 01). N (--Nitro--L--Arginine (L--NNA )which could decrease theproduction of NO by inhibiting nitric oxide synthase (NOS) and antagonize the inhibitory effect of LPS. Thecontents of NO3, NO2 in medium were markedly increased in LPS group (P <0. 01), 48 h group vs 24 h groupincreased by 9. 1%, 72 h group vs 48 h group increased by 4. 5%. In high dose LPS group, positiveimmunohistochemical staining for inducible nitric oxide synthase was observed. Results:It was demonstrated thatlow dose LPS stimulated the proliferation and DNA synthesis of VSMC, while LPS at high dose markedly inhibitedthe proliferation, DNA synthesis and viability of VSMC. Conclusion: The inhibition of VSMC proliferationinduced by LPS is probably due to the increased release of NO by VSMC.
基金This work was supported by the Science and Technology Committee of Guangdong Province ( No. 99M04808G C31203) National Science Foundation of Guangdong Province ( No. 013141)
文摘目的:研究按摩对大鼠骨骼肌急性损伤修复过程中肌卫星细胞激活关键因子神经型一氧化氮合成酶(n NOS)、肝细胞生长因子(HGF)m RNA表达的影响,探讨按摩对于骨骼肌急性损伤修复的作用。方法:将44只SPF级成年雄性SD大鼠按随机抽样法分为3组,正常对照组(A组,n=4),自然恢复组(C组,n=24),按摩组(M组,n=16)。A组不做任何处理,C、M组用改良打击器制备大鼠腓肠肌急性损伤模型。C组不给予按摩,M组于造模后第3天介入按摩治疗。C、M组于造模后第7天、第14天、第21天及第28天取实验动物腓肠肌样本,HE染色观察组织病理改变,实时荧光PCR检测肌卫星细胞中n NOS、HGF m RNA表达量。结果:按摩干预治疗后,M、C组与A组比较,各时间点n NOS、HGF m RNA表达量均高于A组,且具有显著差异(P<0.01);M组与C组比较,在7天、第14天、第21天及第28天n NOS、HGF m RNA表达量M组均高于C组(P<0.01)。M组与C组比较,损伤区域成肌细胞更多,肌丝稠密,肌卫星细胞增殖明显,血供更丰富,坏死组织恢复更快。结论:按摩可有效提高肌卫星细胞中n NOS、HGF m RNA表达水平,激活更多的肌卫星细胞,促进受损骨骼肌的修复再生。
