Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agr...Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.展开更多
Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, ...Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, 1-1-200 and 150-1-5）, wheat （Triticum aestivum L.） and garden cress （Lipidium sativum L.）. This experiment was conducted in laboratory at room temperature. Oil extracted from crude oil contaminated soil was added to sand used in different concentrations （0%, 2%, 4%, 6%, 8% and 10%）. 10 seeds of each crop were sown in ptri-dishes with different treatments separately. For Each treatment four replicates were used in completely randomized design, germination percent was calculated, after one week, shoot and root length were measured. Data were statistically analyzed using MINTAB version 12. The results show that barley mutant line （150-2-3） was significantly different from other entries in germination percent, shoot and root length except mutant line 150-1-5 in germination percent. Results demonstrated that the mutant line 150-2-3 was promising to remediate crude oil contaminated soil.展开更多
Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generation...Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generations..Theleaves of these lines started to become greenafter the fourth leaf extension,and except展开更多
W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the lea...W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the leaves ofW25 shows complete albino, but they exhibitsnormal green when temperature is higher than30℃. Meanwhile, at 25℃, it can be greenable展开更多
Background and Objective Lung cancer is not only the most dangerous threating tumor to humans health and life, but also a malignant tumor with poor prognosis. It has been
基金funded by the Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2020R10310011,2019R1031-12)the National Natural Science Foundation of China(32001577)+4 种基金the Foreign Cooperation Projects for FAAS(DWHZ2021-20)the Opening Foundation of Fujian Key Laboratory for Vegetable Breeding(FJVRC2020-02)the Free Explore Program for FAAS(ZYTS2019007)the Guangxi Natural Science Foundation Program(2018GXNSFDA281027)the Science and Technology Innovative Team in Fujian Academy of Agricultural Sciences(CXTD2021008-3).
文摘Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.
文摘Phytoremediation shows promise as an alternative low-cost to most remediation methods. This study evaluated the germination of six crops, including four mutant lines of barley （Hordium vulgate L.） （1-9-3, 150-2-3, 1-1-200 and 150-1-5）, wheat （Triticum aestivum L.） and garden cress （Lipidium sativum L.）. This experiment was conducted in laboratory at room temperature. Oil extracted from crude oil contaminated soil was added to sand used in different concentrations （0%, 2%, 4%, 6%, 8% and 10%）. 10 seeds of each crop were sown in ptri-dishes with different treatments separately. For Each treatment four replicates were used in completely randomized design, germination percent was calculated, after one week, shoot and root length were measured. Data were statistically analyzed using MINTAB version 12. The results show that barley mutant line （150-2-3） was significantly different from other entries in germination percent, shoot and root length except mutant line 150-1-5 in germination percent. Results demonstrated that the mutant line 150-2-3 was promising to remediate crude oil contaminated soil.
文摘Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generations..Theleaves of these lines started to become greenafter the fourth leaf extension,and except
文摘W25 is a low-temperature-sensitive albino mu-tant line. Temperature not only controls thealbino phenotype expression of W25, but alsodetermines whether it could survive. When thetemperature is lower than 25℃, the leaves ofW25 shows complete albino, but they exhibitsnormal green when temperature is higher than30℃. Meanwhile, at 25℃, it can be greenable
文摘Background and Objective Lung cancer is not only the most dangerous threating tumor to humans health and life, but also a malignant tumor with poor prognosis. It has been
