One of the largest and most important pesticides groups are Demthylation inhibitors, a class of single-side fungicides, used for the control of fungal diseases on fruit. The aim of this study was to investigate the fa...One of the largest and most important pesticides groups are Demthylation inhibitors, a class of single-side fungicides, used for the control of fungal diseases on fruit. The aim of this study was to investigate the fate of myclobutanil after its application in Starking and Golden Delicious cultivars. The apple trees were treated with maximum and minimum levels of recommended doses. The samples were collected randomly in different interval days after application. For identification and quantification of myclobutanil residues in apple fruit liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was performed. The highest values of myclobutanil residues were found at the 1^st day after treatment and by the time the residue levels were decreased. Thus, the level of myclobutanil residues ranges from 0.928 mg kg^-1 to 0.019 mg kg^-1 and from 2.085 mg kg^-1 to 0.086 mg kg^-1 in Starking treated with the minimum and maximum doses respectively. Also, in the Golden Delicious cultivar the level of myclobutanil residues ranges from 0.878 mg kg^-1 to 0.026 mg kg^-1 and from 1.760 mg kg^-1 to 0.092 mg kg^-1 with the minimum and maximum applied doses at 1^st and 40^th day after application, respectively. Therefore, the samples treated with maximum recommended dose did not reach the MRL (0.6 mg kg^-1) before the 14th day after application, whereas the samples treated with minimum recommended dose reached MRL before 7th day after application. Therefore, the application of myclobutanil guarantees food safety for the consumer not only in minimum doses but even in maximal doses as well.展开更多
采用改良的QuEChERS-高效液相色谱-串联质谱(HPLC-MS/MS)技术,建立了荔枝花粉和花蜜中2种主要杀菌剂腈菌唑和苯醚甲环唑的测定方法。花粉和花蜜样品均由乙腈提取,花粉样品经0.9 g无水硫酸镁、0.15 g N-丙基乙二胺(PSA)和0.15 g十八烷基...采用改良的QuEChERS-高效液相色谱-串联质谱(HPLC-MS/MS)技术,建立了荔枝花粉和花蜜中2种主要杀菌剂腈菌唑和苯醚甲环唑的测定方法。花粉和花蜜样品均由乙腈提取,花粉样品经0.9 g无水硫酸镁、0.15 g N-丙基乙二胺(PSA)和0.15 g十八烷基键合硅胶(C_(18))吸附剂净化,花蜜样品由0.9 g无水硫酸镁和0.15 g PSA净化。采用Poroshell-120 EC-C_(18)色谱柱分离,以0.1%(v/v)甲酸水溶液-乙腈(25∶75,v/v)为流动相等度洗脱,在电喷雾离子(ESI)源、正离子扫描和选择离子监测模式下进行检测,基质匹配标准溶液法定量。结果显示:腈菌唑和苯醚甲环唑在1~100μg/L范围内线性关系良好,相关系数(r2)均大于0.999 0;腈菌唑和苯醚甲环唑的检出限(LOD)分别为0.25μg/kg和0.50μg/kg,定量限(LOQ)分别为0.83μg/kg和1.7μg/kg;腈菌唑和苯醚甲环唑在荔枝花粉和花蜜样品中的平均加标回收率分别为87.0%~95.2%和90.1%~96.4%,相对标准偏差(RSD)分别为1.2%~3.6%和0.7%~4.1%。该法快速、简便、灵敏,可用于荔枝花粉和花蜜样品中腈菌唑和苯醚甲环唑的痕量测定,可为蜜蜂等授粉昆虫的暴露性风险评估提供技术支持。展开更多
文摘One of the largest and most important pesticides groups are Demthylation inhibitors, a class of single-side fungicides, used for the control of fungal diseases on fruit. The aim of this study was to investigate the fate of myclobutanil after its application in Starking and Golden Delicious cultivars. The apple trees were treated with maximum and minimum levels of recommended doses. The samples were collected randomly in different interval days after application. For identification and quantification of myclobutanil residues in apple fruit liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was performed. The highest values of myclobutanil residues were found at the 1^st day after treatment and by the time the residue levels were decreased. Thus, the level of myclobutanil residues ranges from 0.928 mg kg^-1 to 0.019 mg kg^-1 and from 2.085 mg kg^-1 to 0.086 mg kg^-1 in Starking treated with the minimum and maximum doses respectively. Also, in the Golden Delicious cultivar the level of myclobutanil residues ranges from 0.878 mg kg^-1 to 0.026 mg kg^-1 and from 1.760 mg kg^-1 to 0.092 mg kg^-1 with the minimum and maximum applied doses at 1^st and 40^th day after application, respectively. Therefore, the samples treated with maximum recommended dose did not reach the MRL (0.6 mg kg^-1) before the 14th day after application, whereas the samples treated with minimum recommended dose reached MRL before 7th day after application. Therefore, the application of myclobutanil guarantees food safety for the consumer not only in minimum doses but even in maximal doses as well.
文摘采用改良的QuEChERS-高效液相色谱-串联质谱(HPLC-MS/MS)技术,建立了荔枝花粉和花蜜中2种主要杀菌剂腈菌唑和苯醚甲环唑的测定方法。花粉和花蜜样品均由乙腈提取,花粉样品经0.9 g无水硫酸镁、0.15 g N-丙基乙二胺(PSA)和0.15 g十八烷基键合硅胶(C_(18))吸附剂净化,花蜜样品由0.9 g无水硫酸镁和0.15 g PSA净化。采用Poroshell-120 EC-C_(18)色谱柱分离,以0.1%(v/v)甲酸水溶液-乙腈(25∶75,v/v)为流动相等度洗脱,在电喷雾离子(ESI)源、正离子扫描和选择离子监测模式下进行检测,基质匹配标准溶液法定量。结果显示:腈菌唑和苯醚甲环唑在1~100μg/L范围内线性关系良好,相关系数(r2)均大于0.999 0;腈菌唑和苯醚甲环唑的检出限(LOD)分别为0.25μg/kg和0.50μg/kg,定量限(LOQ)分别为0.83μg/kg和1.7μg/kg;腈菌唑和苯醚甲环唑在荔枝花粉和花蜜样品中的平均加标回收率分别为87.0%~95.2%和90.1%~96.4%,相对标准偏差(RSD)分别为1.2%~3.6%和0.7%~4.1%。该法快速、简便、灵敏,可用于荔枝花粉和花蜜样品中腈菌唑和苯醚甲环唑的痕量测定,可为蜜蜂等授粉昆虫的暴露性风险评估提供技术支持。
