Expression and functional identification of the hypothetical adhesin P32 from Mycoplasma genitalium

Mycoplasma genitalium is the main causative agent for non-gonococcal and non-chlamydial urethritis. P32 is the putative surface-exposed membrane protein of M. genitalium and it has substaintial identity in amino acid sequence with adhesin protein P30 from M. pneumoniae. Since M. pneumoniae mutants lacking P30 protein is defective in cytadherence, P32 protein has been proposed to be an essential adhesin implicated in the adherence of M. genitaliurn to host cells. The prokaryotic expression vector pET-30 （ ＋ ）/p32 was constructed in the present study, and the recombinant protein was expressed in E. coli and purified under denaturing condition. As demonstrated by the immuno- blotting analysis, the recombinant protein could react with rabbit antisera against M. genitalium, and adherence inhibition assays were performed with antisera against this recombinant protein. It was demonstrated that P32 protein apperared to be an adhesion protein of M. genitalium, thus providing the experimental basis for better understanding of the pathogenesis of M. genitalium infection and for the development of the related vaccines against the infection.

生殖支原体感染的研究进展

生殖支原体(Mg)是一种越来越受到重视的性传播感染病原体。Mg可以引起男性无症状和/或有症状尿道炎,并与女性宫颈炎和盆腔炎密切相关。Mg对HIV感染的获得和传播有重要作用。常用DNA扩增方法检测Mg。Mg感染的治疗可选用阿奇霉素和多西环素。

生殖支原体感染慢性非细菌性前列腺炎患者的病原学的研究

目的用病原学方法探讨慢性非细菌性前列腺炎患者是否存在生殖支原体感染,为临床治疗慢性非细菌性前列腺炎提供有力依据。方法对慢性非细菌性前列腺炎患者,用无菌藻酸钙拭子取前列腺液接种到Mg培养基中,置37℃CO2培养箱中培养,对符合Mg生物学特征的培养物用PCR和电镜加以鉴定。结果在240例慢性非细菌性前列腺炎患者中培养出19株Mg,分离率为7.91%。对照组72例,培养出1株Mg,分离率为1.39%。两组比较有显著性差异(χ2=3.93,P<0.05)。结论从病原学证实,慢性非细菌性前列腺炎患者中存在Mg感染,为阐明慢性非细菌性前列腺炎的病原学提供科学依据。