清热生肌膏介导NLRP3对放射性皮炎大鼠MPO、Hyp表达的影响

目的 清热生肌膏介导NLRP3炎性小体对放射性皮炎大鼠皮肤损伤修复及MPO、Hyp表达水平的影响。方法 选取SD健康大鼠90只,80只随机分为正常组、模型组(放射性皮炎大鼠模型)、治疗组(模型+清热生肌膏)、对照组(模型+复方茶多酚软膏),每组各20只。另设抑制剂组(NLRP3抑制剂)10只。通过创面愈合率衡量创面愈合情况。透射电镜观察创面细胞超微结构。HE染色观察病理学变化。分光光度比色法测定MPO活性。免疫印迹检测NLRP3、Caspase-1蛋白表达。结果 各组在治疗后第1 d时的创面愈合无差异(P>0.05)。模型组大鼠在治疗3~21 d时的创面愈合均低于治疗组及对照组(P<0.05)。治疗组与对照组大鼠治疗治疗3~21 d各时间点创面愈合比较无差异(P>0.05)。与正常组相比,模型组大鼠MPO表达升高、Hyp表达降低(P<0.05)。与模型组相比,治疗组大鼠MPO表达降低、Hyp表达升高(P<0.05)。治疗组与对照组MPO及Hyp表达对比无差异(P>0.05)。与正常组相比,模型组大鼠NLRP3、Caspase-1表达升高(P<0.05)。与模型组相比,治疗组大鼠NLRP3、Caspase-1蛋白表达降低(P<0.05)。治疗组与抑制剂组大鼠NLRP3、Caspase-1蛋白表达对比无差异(P>0.05)。结论 清热生肌膏可抑制MPO水平并促进Hyp表达,对放射性皮炎大鼠皮肤损伤修复有积极促进作用,其作用机制可能与介导NLRP3炎性小体有关。

柴杏颗粒的解热止咳作用及其对肺内MPO、NO、iNOS表达的影响

本研究旨在观察柴杏颗粒的解热止咳作用及其对肺内髓过氧化物酶(MPO)、一氧化氮(NO)水平、诱导型一氧化氮合酶(iNOS)蛋白表达的影响。采用小鼠氨水引咳法,取筛选过的ICR小鼠50只,随机分为正常组,小儿柴桂退热颗粒组(5.2g/kg)、柴杏颗粒高、中、低剂量组(3 g/kg、1.5 g/kg、0.75 g/kg),正常组给予蒸馏水,其他组给予相应药液,连续给药3 d,每天1次,末次药后将小鼠放入浓氨水的烧杯内引咳,记录3min内小鼠的咳嗽潜伏期和咳嗽次数;取合格SD大鼠60只,随机分为正常组、模型组、阿司匹林肠溶片组(0.1 g/kg)、柴杏颗粒高、中、低剂量组(2.1 g/kg、1.05 g/kg、0.525 g/kg),正常组和模型组给予蒸馏水,其他组给予相应药液,灌胃给药,每天1次,连续3 d。末次给药后除正常组皮下注射生理盐水外,其他各组皮下注射干酵母混悬液,检测各组温度变化,处死大鼠,测定肺内MPO活性和NO水平,免疫组织化学法检测iNOS蛋白表达。结果表明,与正常组比较,柴杏颗粒各剂量组均有较好的止咳作用(P<0.01),低剂量组对于干酵母致热大鼠具有很好的解热作用(P<0.01或P <0.001);与正常组比较,模型组MPO活性显著增强(P <0.05),NO水平、iNOS表达显著增加(P<0.001),柴杏颗粒高、低剂量组MPO和NO活性下降(P<0.05或P<0.01);柴杏颗粒各剂量组可显著降低肺泡上皮细胞iNOS的表达量(P<0.001)。柴杏颗粒具有良好的止咳作用,其止咳作用机制与降低肺中MPO和NO活性及iNOS的表达有关。

MPO和Lp-PLA2与急性缺血性脑卒中严重程度的相关性及对早期预后的评估价值

目的探讨髓过氧化物酶(MPO)和脂蛋白相关磷脂酶A2(Lp-PLA2)是否可作为评估急性缺血性脑卒中(AIS)严重程度和早期预后血清标志物。方法选取2020-06—2021-12长治医学院附属和平医院诊治的AIS患者145例,根据出院后3个月改良mRS评分将患者分为预后良好组(mRS≤2分,80例)和预后不良组(mRS>2分,65例)。采用酶联免疫吸附试验(ELISA)检测MPO和Lp-PLA2水平,Pearson相关性分析MPO和Lp-PLA2与NIHSS评分的相关性。Logistic多因素回归分析影响AIS早期预后不良的独立危险因素,ROC曲线分析美国国立卫生研究院卒中量表(NIHSS)评分、脑梗死体积、MPO和Lp-PLA2对AIS早期预后不良的预测价值。结果预后不良组NIHSS评分、肌酸激酶同工酶(CK-MB)、肌钙蛋白T(TnT)、肌钙蛋白I(TnI)、C反应蛋白(CRP)、MPO和Lp-PLA2水平高于预后良好组。MPO、Lp-PLA2水平与NIHSS评分呈正相关(r=0.835、0.796,均P<0.001)。NIHSS评分(OR=2.521,95%CI:1.414~3.950,P=0.015)、脑梗死体积(OR=1.965,95%CI:1.042~3.014,P=0.009)、MPO(OR=3.695,95%CI:1.362~5.263,P<0.001)和Lp-PLA2(OR=2.154,95%CI:1.063~5.217,P<0.001)为影响AIS早期预后不良的独立危险因素。MPO对AIS早期预后不良的预测价值高于NIHSS评分、脑梗死体积(Z=3.514,P=0.020;Z=3.865,P=0.008),Lp-PLA2对AIS早期预后不良的预测价值高于NIHSS评分、脑梗死体积(Z=3.625,P=0.018;Z=4.014,P=0.005),MPO和Lp-PLA2对AIS早期预后不良的预测价值差异无统计学意义(Z=1.025,P=0.109)。结论MPO和Lp-PLA2在AIS早期预后不良患者中表达水平较高,与神经损伤程度呈正相关,与患者早期预后不良有关,有望成为评估AIS严重程度和早期预后不良的血清标志物。

心搏骤停心肺复苏成功患者血清氧化应激因子、MPO、Cys C水平与近期临床预后的关系

目的研究心搏骤停心肺复苏(CPR)成功患者血清氧化应激因子、髓过氧化物酶(MPO)、胱抑素C(Cys C)水平与近期临床预后关系。方法选取80例心搏骤停CPR成功患者,根据6个月的预后情况分为预后良好组(n=38)和预后不良组(n=42),比较两组血清氧化应激因子[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)]、MPO、Cys C水平,采用logistic回归分析血清MDA、SOD、GSH-Px、MPO、Cys C水平与近期临床预后的相关性,并分析各指标对近期临床预后的预测价值。结果预后不良组患者CPR成功后平均动脉压(MAP)低于预后良好组(t=2.54,P<0.05),预后不良组患者CPR成功后30 min、24 h、48 h的血清MDA、MPO、Cys C水平均高于预后良好组(t分别=5.20、4.46、6.23、6.66、3.47、8.37、9.47、14.39、27.59,P均<0.05),SOD、GSH-Px水平均低于预后良好组(t分别=-11.37、-6.16、-5.60、-12.39、-5.65、-5.19,P均<0.05)。logistic回归分析显示,CPR后24 h血清MDA、SOD、GSH-Px、MPO、Cys C水平为心搏骤停CPR成功患者近期临床预后不良的危险因素(OR分别=1.55、1.53、1.54、1.59、1.61,P均<0.05)。ROC曲线分析显示,CPR成功后24 h血清MDA、SOD、GSH-Px、MPO、Cys C水平联合预测心搏骤停CPR成功患者近期临床预后不良的灵敏度为96.00%,AUC为0.91,均高于单独检测(Z分别=8.99、18.98、16.13、7.04、12.12,P均<0.05)。结论血清MDA、SOD、GSH-Px、MPO、Cys C水平是心搏骤停CPR成功患者近期临床预后不良的危险因素,且其水平联合预测心搏骤停CPR成功患者近期临床预后不良的价值较高。

SOD、GSH-Px、MDA、MPO与脑血管病后癫痫发生的预测价值

目的探讨超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、髓过氧化物酶(MPO)与脑血管病后癫痫发生的预测价值。方法选取2018年6月—2023年6月贺兰县人民医院收治的180例脑血管疾病患者为研究对象,依照脑血管疾病发生之后是否出现癫痫进行分组,分为癫痫组(n=28)和非癫痫组(n=152),另选取同期来贺兰县人民医院体检的30名健康志愿者作为对照组。对比三组受检者SOD、GSH-Px、MDA、MPO表达水平,应用Logistic回归分析法分析SOD、GSH-Px、MDA、MPO对脑血管病后癫痫发生的预测价值。随后将28例脑血管病后癫痫患者依照发作类型分为两个亚组,即部分性发作组(n=15)和全身强直痉挛发作组(n=13),对比两组患者SOD、GSH-Px、MDA、MPO表达水平,并采用Spearman相关分析方法分析SOD、GSH-Px、MDA、MPO与脑血管病后癫痫严重程度的相关性。结果癫痫组SOD、GSH-Px水平明显低于非癫痫组和对照组,MDA、MPO水平明显高于非癫痫组和对照组,差异有统计学意义(P<0.05);Logistic回归分析结果显示:SOD、GSH-Px降低和MDA、MPO升高为脑血管病后癫痫发作的独立影响因素(P<0.05);全身强直痉挛发作组患者SOD、GSH-Px水平低于部分性发作组,MDA、MPO水平高于部分性发作组,差异有统计学意义(P<0.05);Spearman相关分析结果显示:SOD、GSH-Px与脑血管病后癫痫严重程度呈负相关,MDA、MPO与脑血管病后癫痫严重程度呈正相关(P<0.05)。结论通过观察血清SOD、GSH-Px、MDA、MPO水平可预测脑血管病后癫痫的发生,且SOD、GSH-Px水平越低,MDA、MPO水平越高,患者癫痫发作情况越严重,因此临床上针对此类脑血管病患者需及时采取相关措施进行干预,预防癫痫发生,并减轻脑血管病后癫痫发作的严重程度。

MPOS联邦实时组合算法

微小型位置姿态测量系统(micro position and orientation system,MPOS)是为成像载荷提供实时高精度运动补偿信息的关键装置,其测量精度严重制约成像精度的提升。针对MPOS集中滤波实时性差的问题,在基于双ARM(advanced reduced instruction set computing machines)硬件平台的基础上,采用联邦滤波实时组合算法对多组传感器数据进行最优信息融合。以微惯性测量单元为公共参考系统,双天线全球导航卫星系统(global navigation satellite system,GNSS)和磁强计作为子系统提供量测信息,将各个子系统得到的局部误差协方差及状态估计值在主滤波器中进行信息融合得到最优估计值。通过动态实验验证了基于联邦滤波实时组合方法的MPOS,其位置、速度、航向角及水平姿态角精度可达0.6 m,0.03 m/s,0.15°,0.04°。

Pathogenic role of myeloperoxidase in acute pancreatitis

BACKGROUND:Myeloperoxidase(MPO)has been implicated in promoting tissue damage in various inflammatory diseases.However,MPO blood levels in relation to the severity of acute pancreatitis(AP)and its time-course have not been studied.The present study aimed to determine the role of MPO in AP.METHODS:We studied 86 patients with AP(48 patients with mild and 38 with severe pancreatitis)and 18 controls(volunteers).The relations of serum MPO levels to cytokine level,severity,and time-course of pancreatitis were studied.The serum level of MPO and cytokines were measured by MPO-EIA and cytokines ELISA,respectively.RESULTS:The highest level of MPO was noted at the first day in patients with severe AP.A decrease of MPO blood level occurred during the first three days in all patients with necrotizing pancreatitis.The development of pancreatitis-associated lung injury and purulent complications was accompanied by increased MPO levels.Administration of pentoxifylline significantly reduced the MPO blood level,which was clearly correlated with the levels of proinflammatory cytokines in the two groups of patients.CONCLUSIONS:The results of the present study showed the MPO blood level is dependent on the severity of AP and on cytokine blood levels.Pentoxifylline in the complex management of severe AP may improve the results of treatment.

Myeloperoxidase and High-Sensitivity C-Reactive Protein for Predicting Major Adverse Cardiovascular Events in Patients with Coronary Heart Disease

Background: Research has shown that high-sensitivity C-reactive protein (hs-CRP) is a major inflammatory marker for prediction of acute coronary syndrome (ACS). Myeloperoxidase (MPO) also plays an important role in atherosclerosis initiation and development. In present study, the major adverse cardiovascular events (MACEs) of patients with coronary heart disease (CHD) were investigated. Methods: MPO, hs-CRP and ACS-related risk factors from 201 ACS (78 AMI and 123 UAP) and 210 non-ACS (84 SAP and 126 non-CHD) patients confirmed by coronary angiography were detected, and the data were analyzed with receiver operating characteristic (ROC) curve and Spearman’s correlation coefficients. MACEs of 285 CHD patients were investigated during the 4-year period follow-up from March 2010 to May 2014. Results: The areas under ROC curve for diagnosing ACS were 0.888 (95% CI 0.843 - 0.933) for MPO, and 0.862 (95% CI 0.815-0.910) for hs-CRP, respectively. There were significantly correlations between MPO and hs-CRP in both ACS and non-ACS groups. Regarding to ACS patients, both MPO and hs-CRP were positively correlated with BMI, TC, TG, LDL-C and Hcy. Prospective study demonstrated that the incidences of MACEs associated significantly with elevated MPO baseline level (yes vs no, OR 7.383, 95% CI 4.095 - 13.309) and high hs-CRP baseline level (yes vs no, OR 4.186, 95% CI 2.469 - 7.097) in CHD patients. Conclusions: The present study provides the epidemiological evidence that elevated baseline MPO and hs-CRP levels are both valuable predictors of MACEs in CHD patients. MPO and hs-CRP would prompt the progression of atherosclerosis and development from SAP to ACS.

Clinical Significance of a Myeloperoxidase Gene Polymorphism and Inducible Nitric Oxide Synthase Expression in Cirrhotic Patients with Hepatopulmonary Syndrome

The clinical significance of a myeloperoxidase (MPO) gene polymorphism and inducible nitric oxide synthase (iNOS) expression in cirrhotic patients with hepatopulmonary syndrome (HPS) was explored. Enrolled subjects were divided into three groups according to their disease/health conditions: the HPS group (cirrhotic patients with HPS; n=63), the non-HPS group (cirrhotic patients without HPS; n=182), and the control group (healthy subjects without liver disease; n=35). The distribution of the MPO–463 G/A genotype and its relationship with iNOS expression in a typical cell block from ascitic fluid were detected by immunohistochemistry and polymerase chain reaction-restricted fragment length polymorphism analysis (PCR-RFLP). In the HPS group, the partial pressure of oxygen in blood and ascitic fluid was significantly decreased (8.95±1.58 kPa and 6.81±0.95 kPa, respectively; both P<0.01), while the partial pressure of carbon dioxide significantly increased (4.62±0.20 kPa and 5.92±0.45 kPa, respectively; P<0.01). MPO and iNOS levels were significantly increased in the HPS group as compared with the non-HPS group. These increases were even more remarkable in ascitic fluid (41.36±11.62 and 13.23±4.81 μg/L; 10.27± 3.20 and 4.95±1.12 μg/L) than in blood (16.66±5.24 and 4.87±1.73 μg/L; 5.79±2.31 and 2.35±0.84 μg/L). The distribution of the MPO genotypes GG, GA, and AA were 76.2%, 22.2% and 1.6% in the HPS group, and 57.7%, 37.9% and 4.4% in the non-HPS group (P<0.05). The expression of iNOS was significantly higher in patients with the G alleles (G/G and G/A) (61.54%, 48/78) than in patients with A alleles (G/A and A/A) (38.46%, 30/78) (P<0.01). It was suggested that the expression levels of iNOS and MPO were correlated with HPS-induced hypoxemia. The MPO-463 G/A mutation might be a protective factor that prevents the development of HPS. The MPO might be involved in the regulation of iNOS expression. In humans, MPO pathways, the iNOS/NO system, and their interaction might have an impact on the occurrence and development of HPS.

Early cardiopulmonary resuscitation on serum levels of myeloperoxidase,soluble ST2,and hypersensitive C-reactive protein in acute myocardial infarction patients

BACKGROUND Prompt and effective cardiopulmonary resuscitation(CPR)can promote the recovery of spontaneous circulation to some extent and can save patients’lives.The minimum target of cardiac resuscitation is the restoration of spontaneous circulation(ROSC).However,owing to prolonged sudden cardiac arrest,there is relatively high mortality within 24 h after cardiac resuscitation.Moreover,severe cerebral anoxia can deteriorate the prognosis of patients.Therefore,it is important to adopt an effective clinical evaluation of acute myocardial infarct(AMI)patients’prognosis after cardiac resuscitation for the purpose of prevention and management.AIM To investigate early CPR effects on human myeloperoxidase(MPO),soluble ST2(sST2),and hypersensitive C-reactive protein(hs-CRP)levels in AMI patients.METHODS In total,54 patients with cardiac arrest caused by AMI in our hospital were selected as the observation group,and 50 other patients with AMI were selected as the control group.The differences in serum levels of MPO,sST2,and hs-CRP between the observation group and the control group were tested,and the differences in the serum levels of MPO,sST2,and hs-CRP in ROSC and non-ROSC patients,and in patients who died and in those who survived,were analyzed.RESULTS Serum levels of MPO,sST2,hs-CRP,lactic acid,creatine kinase isoenzyme(CKMB),and cardiac troponin I(cTnI)were significantly higher in the observation group than in the control group(P<0.05).Serum levels of MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI in the observation group were lower after CPR than before CPR(P<0.05).In the observation group,MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI serum levels were lower in ROSC patients than in non-ROSC patients(P<0.05).MPO,sST2,hs-CRP,and lactic acid serum levels of patients who died in the observation group were higher than those of patients who survived(P<0.05).The areas under receiver operating characteristic curve predicted by MPO,sST2,hs-CRP,lactic acid,CK-MB,and cTnI were 0.616,0.681,0.705,0.704,0.702,and 0.656,respectively(P<0.05).The areas under receiver operating characteristic curve for MPO,SST2,hs-CRP,and lactic acid to predict death were 0.724,0.800,0.689,and 0.691,respectively(P<0.05).Logistic regression analysis showed that MPO,sST2,and hs-CRP were the influencing factors of ROSC[odds ratios=1.667,1.589,and 1.409,P<0.05],while MPO,sST2,hs-CRP,and lactic acid were the influencing factors of death(odds ratios=1.624,1.525,1.451,and 1.365,P<0.05).CONCLUSION Serum levels of MPO,sST2,hs-CRP,and lactic acid have a certain value in predicting recovery and prognosis of patients with ROSC.

CLINICAL AND PATHOLOGICAL MANIFESTATI-ONS OF PATIENTS WITH ANTINEUTROPHIL CYTO-PLASMIC AUTOANTIBODIES DIRECTED AGA INST PROTEINASE 3 OR MYELOPEROXIDASE

To compare the clinical and pathological manifestations of patients with antineutrophil cytoplasmic autoantibodies (ANCA) directed against proteinas e 3 (anti PR3) or myeloperoxidase (anti MPO). Methods. One hundred and forty patients with ANCA were detected for anti PR3 a nd anti MPO by ELISA. The clinical features at presentation, histopathological characteristics and outcome of all patients who were tested positive for anti P R3 or anti MPO were analysed.Results. In anti PR3 group (n=21), 16 cases (76.2%) had systemic vasculitis , in which Wegener’s granulomatosis prevailed (13 cases, 61.9%). In anti MPO g roup (n=31), 19 cases (61.3%) were diagnosed as systemic vasculitis and 12 case s (38.7%) as microscopic angiitis. For vasculitic patients with anti PR3 and a nti MPO, the disease duration at diagnosis was 9.6±2.0m and 4.4±0.9m respecti vely, P< 0.05;vasculitis activity index (BVAS) and mean number of affected organ were 22.5±2.1, 5.0±0.4 and 25.1±1.7, 4.8±0.4 respectively, P >0.05;upper r espiratory tract, eye and joint involvements were 11(68.8%), 7(43.8%), 11(68.8 %) and 7(36.8%), 2(10.5%), 5(26.3%) respectively, P< 0.05.Although there was no statistical difference in renal involvement between these two groups, patien ts with serum creatine >500 μmol/L were more commonly seen in anti MPO group t han in anti PR3 group, which were 8(42.1%) and 2(12.5%) respectively, P< 0.05 . Ten relapses were seen in anti PR3 group and only 2 in anti MPO group, but t he acute mortality rate in anti MPO group (5/19, 27.4%) was much higher than t hat in anti PR3 group (1/16, 6.3%). Conclusions. Anti PR3 and anti MPO occurred mainly in systemic vasculitis. A large divergence was seen in the disease spectrum between patients with anti PR 3 and those with anti MPO. In particular, upper respiratory tract, eye and join t involvements, granuloma formation and relapse were more prominent in anti PR3 patients. By contrast, the anti MPO patients had a more acute disease onset, m ore rapid progressive renal involvement and a higher acute mortality rate.

中性粒细胞哮喘可溶性MPO、IL-8水平及肺功能特征分析

目的:观察中性粒细胞哮喘(NA)患者诱导痰、外周血中性粒细胞(NEU)髓过氧化物酶(MPO)、白介素8(IL-8)水平、肺功能的特征及其规范治疗后的变化。方法:选取2018年1月至2022年12月本院收治的47例未经规范治疗的慢性持续期哮喘患者,分为NA组(18例)和非NA组(NNA组,29例)。分别于治疗前和治疗1个月后行哮喘控制测试(ACT)评分、肺功能检测,肺功能指标包括第1秒用力呼气容积占预计值百分比(FEV1%)、第1秒用力呼气容积占用力肺活量百分比(FEV1/FVC%)、75%和50%的最大呼气中期流速(MMEF75%/25%)。采用酶联免疫吸附试验(ELISA)法检测外周血和诱导痰MPO、IL-8水平。采用Pearson相关分析法分析各指标的相关性。结果:治疗前,NA组FEV1%、FEV1/FVC%、MMEF75%/25%均低于NNA组(P<0.05),ACT无明显差异(P>0.05),治疗后两组上述指标均较治疗前升高(P<0.05)。治疗前、后NA组诱导痰MPO、IL-8水平和NE%均高于NNA组(P<0.05),治疗后NA组诱导痰IL-8水平、NE%下降(P<0.05),MPO无变化(P>0.05)。治疗前NA组外周血IL-8水平高于NNA组(P<0.05),治疗后外周血IL-8水平显著降低(P<0.05),而MPO、NE%无变化(P>0.05)。两组治疗前痰NE%与FEV1%、MMEF75%/25%呈正相关关系,外周血MPO水平与IL-8呈正相关关系,与ACT评分呈负相关关系(均P<0.05);治疗后,外周血MPO水平与IL-8呈正相关关系(P<0.05)。结论:NA患者痰和外周血MPO、IL-8水平高于NNA患者,肺功能较差;MPO、IL-8与NA肺功能、哮喘控制水平相关,监测MPO、IL-8可指导NA治疗。

Effects of the myeloperoxidase 463 gene polymorphisms on development of atrophy in H pylori infected or noninfected gastroduodenal disease

AIM: To investigate the relationship between myelo- peroxidase polymorphisms as a host-related factor and atrophy caused by H pylori. METHODS: Our study enrolled 77 patients. Biopsy materials obtained during gastrointestinal endoscopies were evaluated for the presence of H pylori. Polymerase chain reaction-restriction fragment length polymorphism assay was used to characterize myeloperoxidase genotypes. RESULTS: Forty four patients (57.1%) were Hp (+) and 33 (42.9%) were Hp (-). Sixty six (85.7%) had GG genotype, 10 (12.9%) had GA genotype and 1 (1.29%) had AA genotype. The change in atrophy in relation to neutrophil infiltration was significant in Hp (+) patients (P = 0.0001). The change in atrophy in relation to neutrophil infiltration in patients with GG genotype was significant (P = 0.002). However, the change in atrophy in relation to neutrophil infiltration was not significiant in patients with Hp (+) GG genotype (r = 0.066, P = 0.63). CONCLUSION: Myeloperoxidase genotype is critical for development of atrophy in relation to the severity of inflammation. However, it is interesting to note that, H pylori does not show any additive effect on development of atrophy.

Cardiac Myeloperoxidase Activity Is Elevated in Hypertensive Pregnant Rats

Myeloperoxidase（MPO） is released from activated neutrophils. The inflammation in preeclampsia was found to be associated with endothelial dysfunction. We hypothesized that cardiac and circulating MPO levels are elevated in hypertensive pregnancy. Systolic and diastolic blood pressure and heart rate were measured on pregnancy days 14, 16, 18 and 20 in normal pregnant and hypertensive pregnant rats. Left and right ventricle weights, the number of viable fetuses, litter size, fetal and placenta weights were recorded on gestational day 21. Circulating and cardiac MPO activities, soluble fms-like tyrosine kinase-1（sFlt-1） and vascular endothelial growth factor（VEGF） and nitric oxide（NO） were detected. The results showed increases in cardiac（left, but not right ventricle） and circulating MPO activities, and concomitantly lower number of viable fetuses, litter size, and fetal and placenta weights, and decreases in NO in hypertensive pregnant rats. Also, the increases in circulating sFlt-1 and VEGF were found in hypertensive pregnant group. In conclusion, maternal and fetal detrimental changes along with increases in circulating sFlt-1 and VEGF in hypertensive pregnancy may be associated with increases in cardiac and circulating MPO activities, confirming the causative role of inflammatory response in preeclampsia.

Myeloperoxidase: a new target for the treatment of stroke?

Myeloperoxidase is an important inflammatory factor in the myeloid system,primarily expressed in neutrophils and microglia.Myeloperoxidase and its active products participate in the occurrence and development of hemorrhagic and ischemic stroke,including damage to the blood-brain barrier and brain.As a specific inflammatory marker,myeloperoxidase can be used in the evaluation of vascular disease occurrence and development in stroke,and a large amount of experimental and clinical data has indicated that the inhibition or lack of myeloperoxidase has positive impacts on stroke prognosis.Many studies have also shown that there is a correlation between the overexpression of myeloperoxidase and the risk of stroke.The occurrence of stroke not only refers to the first occurrence but also includes recurrence.Therefore,myeloperoxidase is significant for the clinical evaluation and prognosis of stroke.This paper reviews the potential role played by myeloperoxidase in the development of vascular injury and secondary brain injury after stroke and explores the effects of inhibiting myeloperoxidase on stroke prognosis.This paper also analyzes the significance of myeloperoxidase etiology in the occurrence and development of stroke and discusses whether myeloperoxidase can be used as a target for the treatment and prediction of stroke.

Association of the myeloperoxidase ^(468)G→K polymorphism with gastric inflammation and duodenal ulcer risk

AIM: To eluddate the relations between the myeloperoxidase -468G→A polymorphism and the development of duodenal ulcer (DU), and to investigate the impacts of this host genetic polymorphism on the histopathological featuresof Helicobacter pylori ( H pylori)-related gastritis. METHODS: In a case-control study of 115 consecutive DU patients and 182 controls, the myeloperoxidase-468G→A polymorphism was genotyped. Additionally, gastric mucosal changes were examined according to the updated Sydney System.RESULTS: The two study groups differed in the distributionsof myelperoxidase genotypes (P= 0.008). All six individuals carrying myeloperoxidase A/A genotypes were in the DU group. The carriage of myeloperoxidase allele A and H pylori infection were associated with an increased risk of DU with odds ratios (OR) of 2.3 and 5.8, respectively. Thecombined risk of the carriage of myeloperoxidase allele A and H pylori infection for DU was 8.7 (95% CI, 3.5-21.8). In the H pylori-infected individuals, allele A carriers displayed higher bacterial density scores (P = 0.04) inthe antrum than did non-carriers.CONCLUSION: This work verifies for the first time the association of myeloperoxidase-468G→A polymorphism with antral H pyloridensity and DU disease. The mechanisms underlying this genetic polymorphism in developing DU disease merit further investigations.

FT Ⅰ, a novel positive myeloid-lineage-specific transcription regulatory element within the mouse myeloperoxidase gene enhancer, En 1

FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+ cells have higher binding activity to FT Ⅰ and express the proteins which could form the unique DNA-protein com-plex(es) of FT Ⅰ;. 3, The essential sequence for the specific DNA-protein interactions of FT Ⅰ is AAAAGGGGAAGC; 4, South-western analysis in conjunction with the compe-tition assay of the proteins binding to FT Ⅰ, has revealed a 28 kd protein in WEHI 3BD+ cells that displays the properties of the putative transcription factor which acts through FT Ⅰ. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT Ⅰ.

PLGF HMGB1及MPO水平对妊娠期高血压疾病的诊断价值研究

目的:探讨胎盘生长因子(PLGF)、高迁移率族蛋白B1(HMGB1)及髓过氧化物(MPO)水平在妊娠期高血压疾病中的诊断价值。方法:选取妊娠期高血压疾病患者112例作为观察组,同时选取健康孕妇112例作为对照组,比较各血清PLGF、HMGB1及MPO水平差异,同时分析观察组不同严重程度、妊娠结局患者血清PLGF、HMGB1及MPO水平差异,以及PLGF、HMGB1及MPO诊断重度子痫前期、不良妊娠结局的价值。结果:观察组血清PLGF为(46.63±9.97)pg/mL,低于对照组(P<0.05),而HMGB1和MPO分别为(60.43±11.41)mmoL/L和(14.46±1.38)ng/L,高于对照组(P<0.05)。与妊娠期高血压和轻度子痫前期患者比较,重度子痫前期患者血清PLGF为(37.12±8.85)pg/mL,水平较低(P<0.05),而HMGB1和MPO分别为(72.78±9.83)mmoL/L和(16.09±1.40)ng/L,水平较高(P<0.05)。观察组有不良妊娠结局患者血清PLGF为(40.41±8.21)pg/mL,低于无不良妊娠结局患者(P<0.05),而HMGB1和MPO分别为(69.56±8.97)mmoL/L和(16.12±1.97)ng/L,高于无不良妊娠结局患者(P<0.05)。血清PLGF、HMGB1、MPO诊断重度子痫前期的ROC曲线下面积分别为0.723、0.805、0.869,P<0.05。血清PLGF、HMGB1、MPO预测妊娠高血压患者不良妊娠结局的ROC曲线下面积分别为0.797、0.829、0.751,P<0.05。结论:妊娠期高血压疾病患者血清PLGF水平降低,而HMGB1及MPO水平升高,与疾病严重程度及不良妊娠结局有一定关系,同时在诊断重度子痫前期、预测不良妊娠结局方面有一定价值。

Urinary Myeloperoxidase to Creatinine Ratio as a New Marker for Diagnosis of Urinary Tract Infection

Objective To determine whether urinary myeloperoxidase to creatinine ratio(MCR)can serve as a marker for diagnosis of urinary tract infection(UTI).Methods Patients suspected of UTI were consecutively enrolled and further divided into the culture positive and the sterile groups according to urine culture results.Subsequently,MCR,white blood cell(WBC)and bacteria in the urinary samples from patients were detected and compared between the two groups.Results Finally,253 patients were enrolled including 157 urine culture positive patients and 96 urine culture negative patients(sterile group).After logarithmic transformation in 2 as the base,the MCR,WBC,and bacteria were separately presented as log2 MCR,log2 WBC(quantitative),and log2 bacteria.The values of log2 MCR(8.6±2.5 vs.5.4±1.5,t=-12.453,P=0.001),log2 WBC(quantitative)(8.0±2.5 vs.5.2±1.8,t=-10.332,P=0.001),log2 bacteria(11.4±2.5 vs.8.2±2.8,t=-9.297,P=0.001)and WBC(semi-quantitative)[2(interquartile range 1,3)vs.1(interquartile range 0.5,1),Z=-7.580,P=0.001]showed significant difference between the urine culture positive group and the sterile group.Among the urine culture positive group,the values of log2 MCR of the gram positive and gram negative subgroups were 7.2±2.5 and 9.0±2.4(t=4.016,P=0.001),respectively.The correlation between log2 MCR and log2 WBC(quantitative),log2 bacteria,WBC(semi-quantitative)was 0.708(Pearson correlation,P=0.001),0.381(Pearson correlation,P=0.001),and 0.606(Spearman correlation,P=0.001),respectively.Conclusions MCR is positively correlated with WBC counts and could be served as a promising biomarker for diagnosis of UTI.MCR could be even used for initial inference of infectious bacteria types of UTI.

Serum Myeloperoxidase Level in Systemic Lupus Erythematosus

SYSTEMIC lupus erythematosus （SLE） is a systemicautoimmune disease. Several mechanismshave been put forward as underlying the loss ofself-tolerance and development of organdysfunction, such as genetic, environmental, hormonal andimmunoregulatory factors.