The proliferation of vascular smooth muscle cells (VSMC) plays a role in the maintenance of hypertension and atherosclerosis. Diadenosine polyphosphates have been identified as modulators of the cardiovascular system....The proliferation of vascular smooth muscle cells (VSMC) plays a role in the maintenance of hypertension and atherosclerosis. Diadenosine polyphosphates have been identified as modulators of the cardiovascular system. We detected the existence of diadenosine polyphosphates in the heart and studied their growth stimulating effect on VSMC. Porcine cardiac tissue was deproteinated with perchloric acid and concentrated with a preparative reversed phase C18 column. Then the extract was separated with a size exclusion column, an anion exchange column, and an affinity column. It was then chromatographed with an anion exchange HPLC and a reversed phase HPLC and a purified substance was obtained. Med. Klinik I, Nephrol. Labor, Univ. Klinik Marien hospital, University of Bochum, Herne, Germany (Luo JK, Wang H, Schlüter H, JankowskifJ, Potthoff W, Tepel M and Zidek W) Using the BrdU method, this substance was shown to have a growth stimulating effect that is dose-dependent on VSMC. Matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis showed that the molecular mass of the substance was 757.0, the same as diadenosine triphosphate (AP 3A). UV spectrum indicated the maximum absorption at 259 nm, similar to that of adenosine. Postsource decay (PSD) MALDI MS showed that the substance contained adenine, adenosine, AMP, ADP, and ATP, respectively. Enzymatic cleavage revealed that phosphates in this molecule connected adenosine in 5' position. It was determined from the above data that the substance was Ap 3A. In this study the existence of Ap 3A was demonstrated in the heart. It has a growth stimulating effect on VSMC.展开更多
文摘The proliferation of vascular smooth muscle cells (VSMC) plays a role in the maintenance of hypertension and atherosclerosis. Diadenosine polyphosphates have been identified as modulators of the cardiovascular system. We detected the existence of diadenosine polyphosphates in the heart and studied their growth stimulating effect on VSMC. Porcine cardiac tissue was deproteinated with perchloric acid and concentrated with a preparative reversed phase C18 column. Then the extract was separated with a size exclusion column, an anion exchange column, and an affinity column. It was then chromatographed with an anion exchange HPLC and a reversed phase HPLC and a purified substance was obtained. Med. Klinik I, Nephrol. Labor, Univ. Klinik Marien hospital, University of Bochum, Herne, Germany (Luo JK, Wang H, Schlüter H, JankowskifJ, Potthoff W, Tepel M and Zidek W) Using the BrdU method, this substance was shown to have a growth stimulating effect that is dose-dependent on VSMC. Matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) analysis showed that the molecular mass of the substance was 757.0, the same as diadenosine triphosphate (AP 3A). UV spectrum indicated the maximum absorption at 259 nm, similar to that of adenosine. Postsource decay (PSD) MALDI MS showed that the substance contained adenine, adenosine, AMP, ADP, and ATP, respectively. Enzymatic cleavage revealed that phosphates in this molecule connected adenosine in 5' position. It was determined from the above data that the substance was Ap 3A. In this study the existence of Ap 3A was demonstrated in the heart. It has a growth stimulating effect on VSMC.
