Nanotechnology has emerged as a boon to the society with immense potential in varied area of research and our day-to-day life. The application of nanotechnology for the advancement of biosensor leads to an efficient n...Nanotechnology has emerged as a boon to the society with immense potential in varied area of research and our day-to-day life. The application of nanotechnology for the advancement of biosensor leads to an efficient nanobiosensor with miniature structure as compared to conventional biosensors. Nanobiosensors can be effectively used for sensing a wide variety of fertilizers, herbicide, pesticide, insecticide, pathogens, moisture, and soil pH. Taken together, proper and controlled use of nanobiosensor can support sustainable agriculture for enhancing crop productivity.展开更多
A wavelength-dependent three-dimensional(3D)superlocalization imaging method on gold nanoislands(GNIs)chip was developed as a supersensitive single-molecule thyroid-stimulating hormone(TSH)nanobiosensor.Scattered and ...A wavelength-dependent three-dimensional(3D)superlocalization imaging method on gold nanoislands(GNIs)chip was developed as a supersensitive single-molecule thyroid-stimulating hormone(TSH)nanobiosensor.Scattered and fluorescent signals from gold nanoislands on the substrate and quantum dots(QDs)nanoprobes were simultaneously isolated and acquired within an evanescent field layer generated by total internal reflection(TIR)of incident light using a dual-view device.The 3D TIR fluorescence images of TSH-bound QDs on the GNIs were obtained using z-axis optical sectioning at 10nm intervals before/after immunoreaction to identify the optimal conditions for detection.The localized centroid position of QD nanoprobes and GNI were distinguished at a subdiffraction limit resolution using 3D Gaussian fitting to the point spread function.The QD TSH nanobiosensor using wavelength-dependent 3D TIR fluorescence-based single-molecule localization microscopy(3D TIRF-SLM)imaging technique showed an excellent detection limit of 90 yoctomoles(~54 molecules)and a wide linear dynamic range of 1.14 zmol/L-100 pmol/L for TSH.The detection sensitivity was about 4.4×10^(9)times higher than conventional enzyme-linked immunosorbent assay and could successfully quantify TSH in human serum.The wavelength-dependent 3D TIRF-SLM technique may emerge as a reliable platform for ultrahigh-sensitive nanobiosensors at the single-molecule level and early diagnosis with quantification of disease-related ultra-tracebiomolecules.展开更多
Various nanobiosensors composed of biomaterials and nanomaterials have been developed,due to their demonstrated advantage of showing high performance.Among various biomaterials for biological recognition elements of t...Various nanobiosensors composed of biomaterials and nanomaterials have been developed,due to their demonstrated advantage of showing high performance.Among various biomaterials for biological recognition elements of the nanobiosensor,sensory receptors,such as olfactory and taste receptors,are promising biomaterials for developing nanobiosensors,because of their high selectivity to target molecules.Field-effect transistors(FET)with nanomaterials such as carbon nanotube(CNT),graphene,and conducting polymer nanotube(CPNT),can be combined with the biomaterials to enhance the sensitivity of nanobiosensors.Recently,many efforts have been made to develop nanobiosensors using biomaterials,such as olfactory receptors and taste receptors for detecting various smells and tastes.This review focuses on the biomaterials and nanomaterials used in nanobiosensor systems and studies of various types of nanobiosensor platforms that utilize olfactory receptors and taste receptors which could be applied to a wide range of industrial fields,including the food and beverage industry,environmental monitoring,the biomedical field,and anti-terrorism.展开更多
Nano-sized bacterial pores were inserted into a lipid membrane as a nanobiosensor for the detection of single peptide molecules. Due to the intrinsic properties of single-channel conductance, the transit of individual...Nano-sized bacterial pores were inserted into a lipid membrane as a nanobiosensor for the detection of single peptide molecules. Due to the intrinsic properties of single-channel conductance, the transit of individual molecules through the pore can be studied. The analysis of both the blockage current and duration is able to provide specific structural information and allows the detection of specific peptides in bulk mixtures.展开更多
The emerging nanotechnology has opened novel opportunities to explore analytical applications of the fabricated nano-sized materials. Recent advances in nano-biotechnology have made it possible to realize a variety of...The emerging nanotechnology has opened novel opportunities to explore analytical applications of the fabricated nano-sized materials. Recent advances in nano-biotechnology have made it possible to realize a variety of enzyme electrodes suitable for sensing application. In coating miniaturized electrodes with biocatalysts, undoubtedly the most of the potential deposition processes suffer from the difficulty in depositing process and reproducible coatings of the active enzyme on the miniature transducer element. The promising prospects can concern to the obtaining of thin protein layers by using, i.e. electrochemical deposition, electrophoretic deposition as well as monolayer methods (Langmuir-Blodgett procedure, Layer-by-Layer—LbL). Many aspects dealing with deposition of enzyme by techniques employing electric field are considered, including surface charge of enzyme, and its migration under applied electric filed. The using of nanoscale materials (i.e. nanoparticles, nanowires, nanorods) for electrochemical biosensing has seen also explosive increase in recent years following the discovery of nanotubes. These structures offer a promise in the development of biosensing, facilitating the great improvement of the selectivity and sensitivity of the current methods. Finally, the perspectives in the further exploration of nanoscaled sensors are discussed.展开更多
Since the last decades,nanotechnology has gained the attention of researchers in the field of plant sciences.The characteristics of nanoparticles are reliant on their shape and size.Formulations involving noble metall...Since the last decades,nanotechnology has gained the attention of researchers in the field of plant sciences.The characteristics of nanoparticles are reliant on their shape and size.Formulations involving noble metallic nanoparticles(NMNPs)offer novel technologies to boost agricultural productivity and minimize conventional pesticide use.The application of nanoparticles as nanofertilizer improves plant growth and enhances nutrient uptake efficiency.Noble metallic nanoparticles act as antimicrobial agents against multidrug-resistant pathogens and provide protection to plants.Nanobiosensors and nanodevices are utilized in diverse agro-based areas such as plant disease diagnosis and nanoparticle-based material delivery.Several studies have focused on negative impacts such as toxicity and environmental threats posed by NMNPs.Understanding the effects of NMNPs on agriculture is critical for the assessment of their safety and the impending environmental risks to biodiversity and human health.Here,we summarize the advances of NMNPs towards revolutionizing modern agricultural applications and the recent trends in sustainable precision agriculture.展开更多
文摘Nanotechnology has emerged as a boon to the society with immense potential in varied area of research and our day-to-day life. The application of nanotechnology for the advancement of biosensor leads to an efficient nanobiosensor with miniature structure as compared to conventional biosensors. Nanobiosensors can be effectively used for sensing a wide variety of fertilizers, herbicide, pesticide, insecticide, pathogens, moisture, and soil pH. Taken together, proper and controlled use of nanobiosensor can support sustainable agriculture for enhancing crop productivity.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(Nos.2019R1A2C2002556 and 2020R1C1C1009668)supported by Nano-Material Technology Development Program through the NRF funded by the Ministry of Science,ICT and Future Planning(No.2009-0082580).
文摘A wavelength-dependent three-dimensional(3D)superlocalization imaging method on gold nanoislands(GNIs)chip was developed as a supersensitive single-molecule thyroid-stimulating hormone(TSH)nanobiosensor.Scattered and fluorescent signals from gold nanoislands on the substrate and quantum dots(QDs)nanoprobes were simultaneously isolated and acquired within an evanescent field layer generated by total internal reflection(TIR)of incident light using a dual-view device.The 3D TIR fluorescence images of TSH-bound QDs on the GNIs were obtained using z-axis optical sectioning at 10nm intervals before/after immunoreaction to identify the optimal conditions for detection.The localized centroid position of QD nanoprobes and GNI were distinguished at a subdiffraction limit resolution using 3D Gaussian fitting to the point spread function.The QD TSH nanobiosensor using wavelength-dependent 3D TIR fluorescence-based single-molecule localization microscopy(3D TIRF-SLM)imaging technique showed an excellent detection limit of 90 yoctomoles(~54 molecules)and a wide linear dynamic range of 1.14 zmol/L-100 pmol/L for TSH.The detection sensitivity was about 4.4×10^(9)times higher than conventional enzyme-linked immunosorbent assay and could successfully quantify TSH in human serum.The wavelength-dependent 3D TIRF-SLM technique may emerge as a reliable platform for ultrahigh-sensitive nanobiosensors at the single-molecule level and early diagnosis with quantification of disease-related ultra-tracebiomolecules.
文摘Various nanobiosensors composed of biomaterials and nanomaterials have been developed,due to their demonstrated advantage of showing high performance.Among various biomaterials for biological recognition elements of the nanobiosensor,sensory receptors,such as olfactory and taste receptors,are promising biomaterials for developing nanobiosensors,because of their high selectivity to target molecules.Field-effect transistors(FET)with nanomaterials such as carbon nanotube(CNT),graphene,and conducting polymer nanotube(CPNT),can be combined with the biomaterials to enhance the sensitivity of nanobiosensors.Recently,many efforts have been made to develop nanobiosensors using biomaterials,such as olfactory receptors and taste receptors for detecting various smells and tastes.This review focuses on the biomaterials and nanomaterials used in nanobiosensor systems and studies of various types of nanobiosensor platforms that utilize olfactory receptors and taste receptors which could be applied to a wide range of industrial fields,including the food and beverage industry,environmental monitoring,the biomedical field,and anti-terrorism.
基金Supported by the National Natural Science Foundation of China (Grant No. 20875030) the Shuguang Project of Shanghai (Grant No. 07SG36)
文摘Nano-sized bacterial pores were inserted into a lipid membrane as a nanobiosensor for the detection of single peptide molecules. Due to the intrinsic properties of single-channel conductance, the transit of individual molecules through the pore can be studied. The analysis of both the blockage current and duration is able to provide specific structural information and allows the detection of specific peptides in bulk mixtures.
基金Authors are gratefully acknowledged for financial support of NCN-Grant no.2012/05/B/ST5/00749Wrocław University of Technology.
文摘The emerging nanotechnology has opened novel opportunities to explore analytical applications of the fabricated nano-sized materials. Recent advances in nano-biotechnology have made it possible to realize a variety of enzyme electrodes suitable for sensing application. In coating miniaturized electrodes with biocatalysts, undoubtedly the most of the potential deposition processes suffer from the difficulty in depositing process and reproducible coatings of the active enzyme on the miniature transducer element. The promising prospects can concern to the obtaining of thin protein layers by using, i.e. electrochemical deposition, electrophoretic deposition as well as monolayer methods (Langmuir-Blodgett procedure, Layer-by-Layer—LbL). Many aspects dealing with deposition of enzyme by techniques employing electric field are considered, including surface charge of enzyme, and its migration under applied electric filed. The using of nanoscale materials (i.e. nanoparticles, nanowires, nanorods) for electrochemical biosensing has seen also explosive increase in recent years following the discovery of nanotubes. These structures offer a promise in the development of biosensing, facilitating the great improvement of the selectivity and sensitivity of the current methods. Finally, the perspectives in the further exploration of nanoscaled sensors are discussed.
基金financial assistance (University Grants Commission-Junior Research Fellow,National Testing Agency Reference No.201610181190)Department of Science and Technology,Government of India,for Department of Science and Technology-Innovation in Science Pursuit for Inspired Research (INSPIRE) Ph.D.Fellowship (Junior Research Fellow,INSPIRE Code:IF190457)Science and Engineering Research Board (No.EEQ/2021/000058),Department of Science and Technology,Government of India,financially assisted Amit Kumar Mandal through Empowerment and Equity Opportunities for Excellence in Science scheme
文摘Since the last decades,nanotechnology has gained the attention of researchers in the field of plant sciences.The characteristics of nanoparticles are reliant on their shape and size.Formulations involving noble metallic nanoparticles(NMNPs)offer novel technologies to boost agricultural productivity and minimize conventional pesticide use.The application of nanoparticles as nanofertilizer improves plant growth and enhances nutrient uptake efficiency.Noble metallic nanoparticles act as antimicrobial agents against multidrug-resistant pathogens and provide protection to plants.Nanobiosensors and nanodevices are utilized in diverse agro-based areas such as plant disease diagnosis and nanoparticle-based material delivery.Several studies have focused on negative impacts such as toxicity and environmental threats posed by NMNPs.Understanding the effects of NMNPs on agriculture is critical for the assessment of their safety and the impending environmental risks to biodiversity and human health.Here,we summarize the advances of NMNPs towards revolutionizing modern agricultural applications and the recent trends in sustainable precision agriculture.