Terbium orthophosphate nanoparticles were synthesized using 1-hydroxy ethylidene-l,l-diphosphonic acid（HEDP） as a capping ligand under hydrothermal conditions at 80 ℃. These HEDP-capped TbPO4 nanoparticles owned a ...Terbium orthophosphate nanoparticles were synthesized using 1-hydroxy ethylidene-l,l-diphosphonic acid（HEDP） as a capping ligand under hydrothermal conditions at 80 ℃. These HEDP-capped TbPO4 nanoparticles owned a hexagonal phase structure according to the powder X-ray diffraction（XRD） results and were spherical monodispersed particles with a diameter of about 10 nm confirmed by transimission electron microscope（TEM） images. Interestingly, the luminescent intensities of the HEDP-capped TbPO4 nanoparticles decreased obviously in the presence of Pb2＋ ions and such a quenching behavior of luminescence was well fitted by the Stern-Volmer equation.展开更多
Novel PEI-modified NaBiF4:Yb3+/Er3+upconversion nanoparticles(UCNPs)with hollow structure were prepared by a surface modification free one-step solvothermal method and applied as a luminescent probe to determinate wat...Novel PEI-modified NaBiF4:Yb3+/Er3+upconversion nanoparticles(UCNPs)with hollow structure were prepared by a surface modification free one-step solvothermal method and applied as a luminescent probe to determinate water content in organic solvents.XRD,SEM and HRTEM results demonstrate that the obtained PEI-NaBiF4:Yb3+/Er3+UCNPs are pure hexagonal phase with uniform size.The successful modification of PEI on the UCNPs surface was evidenced byζ-potential test,XPS and TG analysis.These synthesized UCNPs are disintegrated into smaller nanoparticles in the presence of water and thus result in a surface quenching effect,which show the features of 0-100%wide range water response in various organic solvents.The sensing performance towards real samples was validated by the water content determination in beer,rum and white spirit.Furthermore,the luminescence intensity variation of the PEI-modified NaBiF4:Yb3+/Er3+enable the test visualization and ease of portability.展开更多
Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that posses...Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously.Herein,we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles(UCNP)loaded with two dyes for sensitization and quenching of the upconversion luminescence(UCL),respectively.Based on the two independent energy transfer processes of near-infrared(NIR)dye IR845 to UCNP and UCNP to visible dye PAPS-Zn,CIO-and Zn2+were simultaneously detected with a limit of detection(LOD)of41.4 and 10.5 nM,respectively.By tilizing a purpose built 830/980 nm dual-laser confocal microscope,both intrinsic and exogenous CIO and Zn2+in live MCF-7 cells have been accurately quantified.Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells.Through modulation of NIR dye and visible dye with other reactive groups,the nanoprobes can be extended to analyze various intraellular species,which provides a promising tool to study the biological activities in live cells and diagnose diseases.展开更多
基金Supported by the Natural Science Foundation of Shandong Province, China（Nos.ZR2015PB003, ZR2014BM016） and the China Postdoctoral Science Foundation（N o.2015 M572012）.
文摘Terbium orthophosphate nanoparticles were synthesized using 1-hydroxy ethylidene-l,l-diphosphonic acid（HEDP） as a capping ligand under hydrothermal conditions at 80 ℃. These HEDP-capped TbPO4 nanoparticles owned a hexagonal phase structure according to the powder X-ray diffraction（XRD） results and were spherical monodispersed particles with a diameter of about 10 nm confirmed by transimission electron microscope（TEM） images. Interestingly, the luminescent intensities of the HEDP-capped TbPO4 nanoparticles decreased obviously in the presence of Pb2＋ ions and such a quenching behavior of luminescence was well fitted by the Stern-Volmer equation.
基金Project supported by the National Natural Science Foundation of China(21804119,10804099)Key projects of Natural Science Foundation of Zhejiang Province(LZ18B050002).
文摘Novel PEI-modified NaBiF4:Yb3+/Er3+upconversion nanoparticles(UCNPs)with hollow structure were prepared by a surface modification free one-step solvothermal method and applied as a luminescent probe to determinate water content in organic solvents.XRD,SEM and HRTEM results demonstrate that the obtained PEI-NaBiF4:Yb3+/Er3+UCNPs are pure hexagonal phase with uniform size.The successful modification of PEI on the UCNPs surface was evidenced byζ-potential test,XPS and TG analysis.These synthesized UCNPs are disintegrated into smaller nanoparticles in the presence of water and thus result in a surface quenching effect,which show the features of 0-100%wide range water response in various organic solvents.The sensing performance towards real samples was validated by the water content determination in beer,rum and white spirit.Furthermore,the luminescence intensity variation of the PEI-modified NaBiF4:Yb3+/Er3+enable the test visualization and ease of portability.
基金the Science and Technology Cooperation Fund between Chinese and Australian Governments(No.2017YFE0132300)the Strategic Priority Research Program of the CAS(No.XDB20000000)+2 种基金the National Natural Science Foundation of China(Nos.51672272,21771185,21771178,and 21975257)Youth Innovation Promotion Association of CAS(No.2017347)the CAS/SAFEA International Partnership Program for Creative Research Teams.
文摘Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously.Herein,we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles(UCNP)loaded with two dyes for sensitization and quenching of the upconversion luminescence(UCL),respectively.Based on the two independent energy transfer processes of near-infrared(NIR)dye IR845 to UCNP and UCNP to visible dye PAPS-Zn,CIO-and Zn2+were simultaneously detected with a limit of detection(LOD)of41.4 and 10.5 nM,respectively.By tilizing a purpose built 830/980 nm dual-laser confocal microscope,both intrinsic and exogenous CIO and Zn2+in live MCF-7 cells have been accurately quantified.Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells.Through modulation of NIR dye and visible dye with other reactive groups,the nanoprobes can be extended to analyze various intraellular species,which provides a promising tool to study the biological activities in live cells and diagnose diseases.
