A cobalt nanoparticles-attached indium tin oxide（CoNPs/ITO） electrode was applied to the immobilization of hemoglobin（Hb） and an Hb modified CoNPs/ITO electrode（Hb/CoNPs/ITO） was prepared. The direct electron tr...A cobalt nanoparticles-attached indium tin oxide（CoNPs/ITO） electrode was applied to the immobilization of hemoglobin（Hb） and an Hb modified CoNPs/ITO electrode（Hb/CoNPs/ITO） was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H2O2, a mediator-free H2O2 sensor was developed. A linear relationship existed between the catalytic current and the H2O2 concentration in a range of 1.0--100.0 μmol/L with a detection limit （S/N=3） of 0.2μmol/L.展开更多
基金Supported by the National Natural Science Foundation of China（No.20211130505） and the Fundamental Research Funds for the Central Universities of China.
文摘A cobalt nanoparticles-attached indium tin oxide（CoNPs/ITO） electrode was applied to the immobilization of hemoglobin（Hb） and an Hb modified CoNPs/ITO electrode（Hb/CoNPs/ITO） was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H2O2, a mediator-free H2O2 sensor was developed. A linear relationship existed between the catalytic current and the H2O2 concentration in a range of 1.0--100.0 μmol/L with a detection limit （S/N=3） of 0.2μmol/L.
