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2%超分子水杨酸对UVB所致小鼠光老化损伤的修复研究
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作者 孙晓娟 杜晓婕 +2 位作者 周红梅 王瞾 曾维惠 《中国实用医药》 2024年第8期177-180,共4页
目的 探究2%超分子水杨酸对中波紫外线(UVB)所致小鼠光老化损伤的修复作用。方法 选择30只6~8周龄昆明雌鼠,随机分为空白对照组、UVB组、UVB+2%超分子水杨酸组,各10只。空白对照组小鼠正常日光照射饲养, UVB组、UVB+2%超分子水杨酸组隔... 目的 探究2%超分子水杨酸对中波紫外线(UVB)所致小鼠光老化损伤的修复作用。方法 选择30只6~8周龄昆明雌鼠,随机分为空白对照组、UVB组、UVB+2%超分子水杨酸组,各10只。空白对照组小鼠正常日光照射饲养, UVB组、UVB+2%超分子水杨酸组隔日用UVB照射1次,辐照12周,其中UVB+2%超分子水杨酸组小鼠在UVB照射后涂抹1次2%超分子水杨酸,非照射日每日涂抹1次;最后1次辐照结束后48 h观察三组小鼠皮肤组织形态变化情况,应用苏木精-伊红(HE)染色观察小鼠背部皮肤显微结构改变情况,测定表皮、真皮厚度,应用Masson染色观察真皮胶原纤维排列情况,测定胶原纤维含量,用免疫组化染色观察皮肤组织p21、p53表达情况。比较三组小鼠背部皮肤外形情况、皮肤显微结构改变情况及皮肤组织p21、p53阳性表达情况。结果 与空白对照组相比,UVB组小鼠皮肤粗糙,有深皱纹毛孔扩大,缺乏弹性,皮肤呈褐黄色斑,皮革样外观,镜下见毛细血管扩张,结痂,为典型光老化特征;UVB+2%超分子水杨酸组皮肤外观有隐约褐色斑,镜下见皮肤有散在、不规则褐色斑片,光老化症状较轻。三组背部皮肤损伤程度比较有明显差异性(P<0.05);UVB组背部皮肤损伤程度明显大于空白对照组和UVB+2%超分子水杨酸组,有明显差异性(P<0.05);空白对照组与UVB+2%超分子水杨酸组皮损程度比较也有明显差异性(P<0.05)。空白对照组表皮厚度、真皮厚度、真皮胶原纤维含量分别为(18.55±1.80)μm、(350.08±35.16)μm、(0.46±0.05)OD, UVB组分别为(79.68±21.37)μm、(611.62±16.37)μm、(0.15±0.30)OD, UVB+2%超分子水杨酸组分别为(60.99±11.49)μm、(467.94±20.71)μm、(0.32±0.03)OD。三组表皮厚度、真皮厚度及真皮胶原纤维含量比较有明显差异性(P<0.05);UVB+2%超分子水杨酸组和UVB组表皮厚度、真皮厚度明显多于空白对照组,真皮胶原纤维含量显著少于空白对照组,有明显差异性(P<0.05);UVB+2%超分子水杨酸组表皮厚度、真皮厚度明显少于UVB组,真皮胶原纤维含量多于UVB组,有明显差异性(P<0.05)。免疫组化显示空白对照组未见有p21、p53阳性表达。UVB+2%超分子水杨酸组皮肤组织p21、p53阳性表达率分别为30.00%、10.00%,低于UVB组的80.00%、60.00%,有明显差异性(P<0.05)。结论 2%超分子水杨酸对UVB所致小鼠光老化皮肤有修护保护作用,其机制与抑制凋亡相关基因p21、p53表达有关。 展开更多
关键词 皮肤光老化 2%超分子水杨酸 中波紫外线
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真空紫外协同Co^(2+)催化过硫酸氢钾降解罗丹明B 被引量:1
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作者 陈徐庆 唐玉朝 +4 位作者 伍昌年 黄显怀 王坤 朱先胜 陈彩虹 《环境科学研究》 CAS CSCD 北大核心 2024年第4期822-834,共13页
有机染料具有毒性强、色度高、不易降解等特性,为了高效去除有机染料,实验以真空紫外(VUV)为光源,研究了VUV协同Co^(2+)催化过硫酸氢钾(PMS)降解典型有机染料罗丹明B(RhB)的反应机制和转化途径.结果表明:①在RhB初始浓度为80 mg/L,Co^(... 有机染料具有毒性强、色度高、不易降解等特性,为了高效去除有机染料,实验以真空紫外(VUV)为光源,研究了VUV协同Co^(2+)催化过硫酸氢钾(PMS)降解典型有机染料罗丹明B(RhB)的反应机制和转化途径.结果表明:①在RhB初始浓度为80 mg/L,Co^(2+)和PMS投加量分别为15μmol/L、0.5 mmol/L的条件下,VUV/Co^(2+)/PMS体系反应10 min,RhB去除率可达99.1%.VUV/Co^(2+)/PMS体系对RhB降解遵循一级动力学规律,反应速率常数(k)随初始质量浓度的增加而减小.②溶液初始pH对反应速率有较大的影响,随着pH减小,反应速率也同时减小.投加量为30 mmol/L的HCO_(3)^(−)、Cl^(−)均表现出显著的抑制作用,相较于对照组,RhB去除率由99.1%分别降至66.0%、84.2%,而NO_(3)^(−)和SO_(4)^(2−)抑制作用不显著;印染助剂柠檬酸钠也会显著抑制RhB降解.③自由基捕获实验和电子顺磁共振(EPR)测试结果表明,VUV/Co^(2+)/PMS体系中存在的氧化物种包括硫酸根自由基(SO_(4)^(−)·)、羟基自由基(·OH)、单线态氧(1O2).④根据紫外可见吸收光谱和质谱结果,初步推断RhB分子降解主要通过活性氧(ROS)攻击造成共轭结构破坏和N-位脱乙基等作用.另外,对总有机碳(TOC)进行测试,30 min时RhB矿化度可达到43.8%.研究显示,VUV/Co^(2+)/PMS体系能够有效去除RhB. 展开更多
关键词 真空紫外 Co^(2+) 过硫酸氢钾 罗丹明b 活性氧
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丹参素对UVB诱导的皮肤光老化小鼠的保护作用和抗氧化机制研究 被引量:2
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作者 王安娜 方梦婕 +1 位作者 唐超 岳天翔 《日用化学工业(中英文)》 北大核心 2024年第1期65-72,共8页
研究丹参素(DSS)对紫外线B(UVB)诱导的皮肤光老化(SP)小鼠的保护作用和抗氧化机制。建立了UVB诱导的SP小鼠模型,使用3个剂量的DSS(20,40和80 mg/(kg·d))治疗小鼠8周,结束后分别测定了各组小鼠的表皮含水量。通过HE染色评价皮肤组... 研究丹参素(DSS)对紫外线B(UVB)诱导的皮肤光老化(SP)小鼠的保护作用和抗氧化机制。建立了UVB诱导的SP小鼠模型,使用3个剂量的DSS(20,40和80 mg/(kg·d))治疗小鼠8周,结束后分别测定了各组小鼠的表皮含水量。通过HE染色评价皮肤组织形态,Masson三色染色评价胶原蛋白沉积。按照试剂盒说明测定皮肤组织中氧化应激指标(SOD、CAT、GSH-Px和MDA)和炎症指标(TNF-α和IL-6)水平。通过RT-qPCT和Western blotting检测了皮肤组织中MMP-1、Collagen I、Nrf2、Keap1、HO-1、NF-κB p65和p-NF-κB p65的mRNA或蛋白水平。结果显示,DSS剂量依赖性地提高了UVB诱导的SP小鼠表皮含水量,减轻皮肤损伤,促进胶原形成(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中MMP-1的转录和表达,促进了Collagen的转录和表达(P<0.05)。DSS升高了UVB诱导的SP小鼠皮肤组织中SOD、CAT和GSH-Px的水平,降低了MDA的水平(P<0.05)。DSS降低了UVB诱导的SP小鼠皮肤组织中TNF-α和IL-6的水平(P<0.05)。DSS促进了UVB诱导的SP小鼠皮肤组织中Nrf2和HO-1的转录和表达,抑制了Keap1的转录和表达(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中p-NF-κB p65的表达(P<0.05)。本研究表明DSS可有效改善UVB诱导的小鼠SP,其机制与Nrf2和NF-κB信号通路有关。 展开更多
关键词 丹参素 紫外线b 皮肤光老化 小鼠 抗氧化
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6-巯基-5-三唑并[4,3-b]-s-四嗪(MTT)的密度泛函理论研究
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作者 赵宁 陈慧 +2 位作者 任黎英 邵长斌 陈玉锋 《原子与分子物理学报》 CAS 北大核心 2024年第1期37-42,共6页
采用密度泛函理论(Density functional theory,DFT),在B3LYP/6-31g(d)(C,H,N,S),Ag原子采用LanL2d赝势基组水平上对甲醛(HCHO)与4-氨基-5肼基-3-巯基-1,2,4-三唑(4-amino-5-hydrazino-3-mercapto-1,2,4-triazole,AHMT)衍生化反应生的成... 采用密度泛函理论(Density functional theory,DFT),在B3LYP/6-31g(d)(C,H,N,S),Ag原子采用LanL2d赝势基组水平上对甲醛(HCHO)与4-氨基-5肼基-3-巯基-1,2,4-三唑(4-amino-5-hydrazino-3-mercapto-1,2,4-triazole,AHMT)衍生化反应生的成产物6-巯基-5-三唑并[4,3-b]-s-四嗪(6-mercapto-5-triazolo[4,3-b]-s-tetrazine,MTT)及其银配合物进行结构优化,优化结果表明MTT的结构是一个近平面结构.通过对频率计算,获得MTT分子及其银配合物的拉曼光谱,对400-1800 cm^(-1)波段内的拉曼光谱特征峰进行了指认.同时讨论了MTT分子的表面静电势,分析可能发生化学反应的位点.并采用含时密度泛函理论(Time Dependent density functional theory,TDDFT)对MTT分子与Ag3配合物的激发态进行了计算分析,并使用电荷转移光谱对Ag配合物与MTT之间电荷转移关系进行了研究.该研究对MTT分子的光谱测定和电子性质提供了理论基础. 展开更多
关键词 密度泛函理论 拉曼光谱 紫外光谱 6-巯基-5-三唑并[4 3-b]-s-四嗪
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CO_(2)点阵激光联合NB-UVB治疗非节段型白癜风的效果及对心身状态的影响
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作者 张凯 《中国医药科学》 2024年第3期195-198,共4页
目的 探讨CO_(2)点阵激光联合窄谱中波紫外线(NB-UVB)治疗非节段型白癜风(NSV)的效果及对心身状态的影响。方法 选取2022年1月至2023年1月徐州市矿务集团总医院皮肤科门诊治疗的70例NSV患者,采用随机数表分为NB-UVB组(n=35,NB-UVB治疗)... 目的 探讨CO_(2)点阵激光联合窄谱中波紫外线(NB-UVB)治疗非节段型白癜风(NSV)的效果及对心身状态的影响。方法 选取2022年1月至2023年1月徐州市矿务集团总医院皮肤科门诊治疗的70例NSV患者,采用随机数表分为NB-UVB组(n=35,NB-UVB治疗)和联合组(n=35,CO_(2)点阵激光联合NB-UVB治疗)。比较两组临床疗效,白癜风面积及严重程度(VASI)评分,肿瘤坏死因子(TNF-α)、γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)水平,T淋巴细胞亚群(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))水平,以及临床症状自评量表(SCL-90)评分。结果 联合组总有效率高于NB-UVB组,差异有统计学意义(P<0.05)。治疗后,联合组VASI、SCL-90评分以及TNF-α、IFN-γ、CD8^(+)水平低于NB-UVB组,差异有统计学意义(P<0.05);且联合组IL-4、CD4^(+)、CD4^(+)/CD8^(+)水平高于NB-UVB组,差异有统计学意义(P<0.05)。结论 CO_(2)点阵激光联合NB-UVB治疗NSV的效果显著,能抑制炎症反应,调节免疫平衡,改善心身状态。 展开更多
关键词 CO_(2)点阵激光 窄谱中波紫外线 非节段型白癜风 心身状态
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Effect of Ultraviolet Radiation on Hsp70 Protein Expression in HaCaT Cells
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作者 Sergio Hugo Sánchez Rodríguez Jesús Rodríguez Vergil +6 位作者 Manuel Venancio Muñoz Juárez Kevin Said Ramírez Dávila Luis Martín García Ortiz Germán Flores Cortés Luz Elena Vidales Rodríguez Jesús Adrián López David Alejandro García López 《World Journal of Nuclear Science and Technology》 CAS 2024年第1期59-71,共13页
Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with human... Ultraviolet radiation by its wavelength is divided into: UVA, UVB and UVC. Only UVA and UVB manage to penetrate the ozone layer, but due to anthropological activities, all of them are capable of interacting with humans to a greater or lesser extent, and can generate adverse effects such as cellular stress when interacting with intra-and extracellular biomolecules. The skin is the first organ in contact with UV radiation, and the stress it generates can be analyzed by the expression of a bioindicator of cellular damage such as Hsp70. Therefore, the objective of the project was: to determine the effect of UVA, UVB and UVC radiation on HaCaT epithelial cells, by analyzing the expression of Hsp70. Materials and methods: HaCaT cells were cultured in vitro, which were irradiated with UVA, UVB and UVC light at different doses, to subsequently determine the degree of Hsp70 expression by Immunodetection by PAGE-SDS and Western Blot. Results: Basal expression of Hsp70 was observed in no irradiated HaCaT cells. When HaCaT cells were irradiated with UVA, UVB, UVC, an increase in this Hsp70 protein was observed. With UVA, a higher degree of expression was observed at a time of 30 minutes of irradiation. With UVB the highest expression shifted to a time of 20 minutes. With UVC, overexpression was observed after 10 minutes. Conclusion: UV radiation generates cellular stress on HaCaT cells, evaluated by the stress bioindicator Hsp70. According to the wavelength of UV radiation, those that have a shorter wavelength have a greater potential for cellular damage, such as UVC. 展开更多
关键词 ultraviolet A Light (UVA) ultraviolet b Light (UVb) ultraviolet C Light (UVC) Heat Shock Protein 70 (Hsp70) HACAT
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大黄酸通过抑制p38 MAPK磷酸化减轻UVB诱导的皮肤光老化损伤
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作者 邵冠儒 张坤阳 《日用化学工业(中英文)》 CAS 北大核心 2024年第7期836-843,共8页
揭示大黄酸(rhein)对紫外线B(UVB)诱导的皮肤光老化损伤的影响及机制。将大鼠分为6组,分别为正常对照组(NC)、UVB组、低剂量Rhein组(L-Rhein)、中剂量Rhein组(M-Rhein)、高剂量Rhein组(H-Rhein)和高剂量Rhein+p38 MAPK激动剂Anisomycin... 揭示大黄酸(rhein)对紫外线B(UVB)诱导的皮肤光老化损伤的影响及机制。将大鼠分为6组,分别为正常对照组(NC)、UVB组、低剂量Rhein组(L-Rhein)、中剂量Rhein组(M-Rhein)、高剂量Rhein组(H-Rhein)和高剂量Rhein+p38 MAPK激动剂Anisomycin组(H-Rhein+Anisomycin)。NC组大鼠仅剃毛,不照射,其他组进行UVB照射。大黄酸的给药途径为口服。大鼠共治疗8周。治疗结束后,分别测定各组大鼠的体重、表皮含水量、皮肤组织中氧化应激指标水平,并对皮肤组织进行HE染色和Masson三色染色,计算胶原体积分数(CVF)。通过RT-qPCR检测皮肤组织中TNF-α、IL-1β、IL-6、MMP-1、MMP-3和CollagenⅠmRNA水平。通过Western blotting检测皮肤组织中p-p38 MAPK和p38MAPK蛋白水平。结果显示,与UVB组比较,L-Rhein组、M-Rhein组和H-Rhein组大鼠皮肤p38 MAPK磷酸化水平降低,表皮含水量升高,皮肤损伤减轻,CVF升高,皮肤MMP-1和MMP-3 mRNA相对表达量降低,CollagenⅠmRNA相对表达量升高,皮肤SOD、CAT和GSH-Px水平升高,MDA水平降低,皮肤TNF-α、IL-1β和IL-6 mRNA相对表达量降低(P<0.05)。Anisomycin减弱了大黄酸的皮肤保护作用(P<0.05)。说明大黄酸通过抑制p38 MAPK磷酸化减轻UVB诱导的皮肤光老化损伤。因此,大黄酸可能是一种防治皮肤光老化的候选天然药物。 展开更多
关键词 大黄酸 紫外线b 皮肤光老化 p38 MAPK 抗氧化 抗炎
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增强UV-B胁迫对小麦幼苗CaM含量的影响
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作者 张俊红 《园艺与种苗》 CAS 2024年第5期91-93,共3页
[目的]对增强UV-B胁迫下小麦幼苗中钙调素(CaM)含量进行研究,进而研究增强UV-B胁迫下小麦幼苗体内Ca^(2+)-CaM通路的情况。[方法]采用二次盐析、Sephadex G-50凝胶过滤层析等方法,从小麦幼苗中分离纯化的CaM,并采用PDE法测定CaM含量。[... [目的]对增强UV-B胁迫下小麦幼苗中钙调素(CaM)含量进行研究,进而研究增强UV-B胁迫下小麦幼苗体内Ca^(2+)-CaM通路的情况。[方法]采用二次盐析、Sephadex G-50凝胶过滤层析等方法,从小麦幼苗中分离纯化的CaM,并采用PDE法测定CaM含量。[结果]增强UV-B胁迫导致小麦幼苗CaM含量增加了23%,这可能为增强UV-B胁迫导致钙离子增多,进而调控CaM所致。[结论]结果为小麦抗辐射机制的研究奠定了一定的基础。 展开更多
关键词 增强UV-b辐射 CAM 小麦幼苗 PDE法
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The Action of Aloe Anthraquinones in Protecting the Growth of Soybean from Ultraviolet Radiation Stress 被引量:1
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作者 贵梦园 张中田 +3 位作者 张佩佩 韩琼 王庭杰 李景原 《Agricultural Science & Technology》 CAS 2014年第12期2078-2080,共3页
In order to investigate the protective effect of aloe anthraquinones on growth and development of soybean against ultraviolet B radiation stress from the morphological structure and physio-chemical indices. The result... In order to investigate the protective effect of aloe anthraquinones on growth and development of soybean against ultraviolet B radiation stress from the morphological structure and physio-chemical indices. The results showed that, stressed by the enhanced ultraviolet b radiation, the soybean gave a dwarfed plant, shrunken leaf area and decreased photosynthetic pigment, while an ascended MDA content. Spraying aloe anthraquinones effectively relieved the reductions of chloro- phyll content and biomass and decreased the production of MDA under the radia- tion of UV-B. Moreover, under the UV-B radiation, waxy substances on epidermal cells increased remarkably and the stomas showed obvious subsidence, while spraying aloe anthraquinones could maintain the structure and shape of cells similar to that under natural light, and the stomas subsidence as well. 展开更多
关键词 ultraviolet b radiation stress ALOE ANTHRAQUINONES SOYbEAN Protection
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心理疏导联合NB-UVB、火针治疗面部白癜风的疗效观察 被引量:1
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作者 薛新国 《中国实用医药》 2023年第17期129-132,共4页
目的探讨心理疏导联合窄谱中波紫外线(NB-UVB)、火针治疗面部白癜风的疗效。方法70例面部白癜风患者,根据随机数字表法分为观察组与对照组,每组35例。对照组应用NBUVB、火针治疗,观察组在对照组基础上加用心理疏导。比较两组临床疗效、... 目的探讨心理疏导联合窄谱中波紫外线(NB-UVB)、火针治疗面部白癜风的疗效。方法70例面部白癜风患者,根据随机数字表法分为观察组与对照组,每组35例。对照组应用NBUVB、火针治疗,观察组在对照组基础上加用心理疏导。比较两组临床疗效、不良事件发生情况及治疗前后的生活质量[皮肤病生活质量指数量表(DLQI)]、心理健康状况[抑郁自评量表(SDS)、焦虑自评量表(SAS)评分]。结果治疗12周后,观察组总有效率为97.1%,高于对照组的77.1%,差异具有统计学意义(P<0.05)。治疗12周后,对照组SDS、SAS评分与本组治疗前比较差异无统计学意义(P>0.05);观察组SDS、SAS评分低于本组治疗前,差异具有统计学意义(P<0.05)。治疗12周后,观察组SDS、SAS评分分别为(27.92±3.68)、(26.33±3.85)分,低于对照组的(37.92±5.62)、(35.82±4.03)分,差异具有统计学意义(P<0.05)。治疗12周后,两组DLQI评分均低于本组治疗前,且观察组DLQI评分(3.81±0.99)分低于对照组的(6.06±1.21)分,差异具有统计学意义(P<0.05)。两组不良事件发生率比较,差异无统计学意义(P>0.05)。结论采用心理疏导联合NB-UVB、火针治疗面部白癜风效果较好,能够提高患者的生活质量,改善患者的心理健康状况,且治疗方案安全性较高,具有较高的应用及推广价值。 展开更多
关键词 心理疏导 窄普中波紫外线 火针 面部白癜风
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Hydrogen-rich saline protects against ultraviolet B radiation injury in rats 被引量:8
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作者 Ze Guo Bingrong Zhou +2 位作者 Wei Li Xuejun Sun Dan Luo 《The Journal of Biomedical Research》 CAS 2012年第5期365-371,共7页
Exposure of skin to solar ultraviolet(UV) radiation induces photo-damage.Ultraviolet B(UVB) is the major component of UV radiation which induces the production of reactive oxygen species(ROS) and plays an impor-... Exposure of skin to solar ultraviolet(UV) radiation induces photo-damage.Ultraviolet B(UVB) is the major component of UV radiation which induces the production of reactive oxygen species(ROS) and plays an impor-tant role in photo-damage.Hydrogen gas reduces ROS and alleviates inflammation.In this study,we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused by UVB radiation.UVB radiation was irradiated on female C57BL/6 rats to induce skin injury.Hydrogen-rich saline and nitrogen-rich saline were ad-ministered to rats by intraperitoneal injection.Skin damage was detected by microscope after injury.UVB radia-tion had a significant affection in tumor necrosis factor alpha,interleukin(IL)-1β and IL-6 levels,tissue superox-ide dismutase,malondialdehyde and nitric oxide activity.Hydrogen-rich saline had a protective effect by altering the levels of these markers and relieved morphological skin injury.Hydrogen-rich saline protected against UVB radiation injury,possibly by reducing inflammation and oxidative stress. 展开更多
关键词 ultraviolet b photo-damage oxidative stress hydrogen-rich saline SKIN
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Effect of Rare Earths on Plant under Supplementary Ultraviolet-B Radiation: Ⅰ Effect of Cerium on Growth and Photosynthesis in Rape Seedlings Exposed to Supplementary Ultraviolet-B Radiation 被引量:11
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作者 梁婵娟 黄晓华 周青 《Journal of Rare Earths》 SCIE EI CAS CSCD 2005年第5期569-575,共7页
Effect of cerium (Ce^3+) on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation (UV-B, 280 - 320 nm) was studied with hydroponics under laboratory conditions. The growth o... Effect of cerium (Ce^3+) on growth and photosynthesis in rape seedlings exposed to two levels of ultraviolet-B radiation (UV-B, 280 - 320 nm) was studied with hydroponics under laboratory conditions. The growth of rape seedlings exposed to two levels of UV-B irradiation (0.15 and 0.35 W· m^-2/T2) was both heavily restrained. The aboveground growth indices including stem (plant) height, leaf number, leaf area, leaf fresh/dry weight and stem fresh/dry weight were obviously decreased by 13.2% - 44.1% (T1) and 21 .4% - 49.3% (T2). Compared to CK, and except active absorption area of roots, the belowground indices main root length, root volume and fresh/dry weight by 14.1% -35.6% (T1) and 20.3% - 42.6% (T2), respectively. For Ce + UV-B treatments, the aboveground and belowground growth indices were decreased by 4.1% - 23.6%, 5.2% -23.3% (Ce+T1) and 10.8% -28.4%, 7.0% -27.8% (Ce +T2), lower than those of UV-B treatments mentioned above. These results show that Ce has protective effect on plant against injury of UV-B radiation. Furthermore, the protective effect of Ce on seedlings exposed to T1 level of UV-B radiation is superior to T2 level. Chlorophyll content, net photosynthesis rate, transpiration rate, stomatal conductance and water use efficiency in UV-B treatments decrease dramatically, whereas intercellular CO2 concentration increases. Although these indices in Ce + UV-B treatments decrease compared with those of CK, the decrease in Ce + UV-B treatments are lower than those in UV-B treatment. This phenomenon indicates that the ecophysiological protective effect of Ce is based on improving photosynthesis in plants. The dynamic curves of photosynthesis indices show that the course of light-repair is shortened and the injury to rape seedlings by UV-B radiation stress is alleviated by Ce. 展开更多
关键词 CERIUM ultraviolet-b radiation rape seedlings PHOTOSYNTHESIS rare earths
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Effect of Lanthanum on Plants under Supplementary Ultraviolet-B Radiation: Effect of Lanthanum on Flavonoid Contents in Soybean Seedlings Exposed to Supplementary Ultraviolet-B Radiation 被引量:8
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作者 梁滨 黄晓华 +2 位作者 张光生 张峰 周青 《Journal of Rare Earths》 SCIE EI CAS CSCD 2006年第5期613-616,共4页
The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase (PAL) activity in soybean seedlings under supplementary ultraviolet-B radiation (UV-B, 280 - 320 nm) was studied. The results s... The effect of La on flavonoids, chlorophyll contents, and phenylalanine ammonia-lyase (PAL) activity in soybean seedlings under supplementary ultraviolet-B radiation (UV-B, 280 - 320 nm) was studied. The results show that PAL activity, contents of flavonoids and chlorophyll in the plants pretreated with La (20 mg·L^- 1 ) are higher than those in CK. UV-B radiation could result in an increase in flavonoid content and PAL activity, associated with a decrease in chlorophyll content. However, the increase in the range of PAL activity and flavonoid content in UV-B treatment are lesser than those in the La treatment. The changes of flavonoid contents and PAL activity in La + UV-B treatment are similar to those in UV-B treatment, and the increase in their range is higher than those in UV-B treatment. This shows that La can enhance the resistance of soybean seedling to UV-B radiation and alleviate the damage of UV-B radiation by increasing flavonoid content, chlorophyll content, and PAL activity. 展开更多
关键词 LANTHANUM ultraviolet-b radiation soybean seedling flavoniod rare earths
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Influence of lanthanum on chloroplast ultrastructure of soybean leaves under ultraviolet-B stress 被引量:8
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作者 彭倩 周青 《Journal of Rare Earths》 SCIE EI CAS CSCD 2009年第2期304-307,共4页
In order to investigate the effects of lanthanum(Ⅲ) on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation (UV-B, 280-320 nm), the chloroplast ultrastructure of soybean seedlings was st... In order to investigate the effects of lanthanum(Ⅲ) on cell ultrastructure of soybean leaves under elevated ultraviolet-B irradiation (UV-B, 280-320 nm), the chloroplast ultrastructure of soybean seedlings was studied by hydroponics under laboratory conditions. The resuits showed that the thylakoid in chloroplast was orderly and clearly as soybean leaves were pretreated by La(Ⅲ). The thylakoid was indis- tinctly disordered, expanded and even indiscoverable in the chloroplast under UV-B stress. The impact on the thylakoid by the high intensity UV-B irradiation (T2) was bigger than that by the low intensity UV-B irradiation (T1). However, the destruction of the chloroplast structure caused by UV-B stress was alleviated by La(Ⅲ), and the arrangement of the thylakoid in the chloroplast became orderly and clearly. The effect of the alleviation by La(Ⅲ) under the low intensity UV-B irradiation (T1) was better than that under the high intensity UV-B irradiation (T2). 展开更多
关键词 LANTHANUM ultraviolet-b irradiation soybean leaves CHLOROPLAST ULTRASTRUCTURE rare earths
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低温治疗通过p38 MAPK信号通路抑制UVB诱导的小鼠皮肤炎症 被引量:1
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作者 骆青燕 梁丽娜 +1 位作者 吴实 吉苏云 《免疫学杂志》 CAS CSCD 北大核心 2023年第6期500-506,共7页
目的探讨局部低温治疗对中波紫外线辐射(UVB)诱导的皮肤炎症的作用机制。方法角质形成细胞体外培养后接受如下3种处理:仅低温治疗、仅UVB照射、UVB照射后低温治疗,检测低温治疗对UVB照射后角质形成细胞的作用。将野生型雌性C57BL/6小鼠... 目的探讨局部低温治疗对中波紫外线辐射(UVB)诱导的皮肤炎症的作用机制。方法角质形成细胞体外培养后接受如下3种处理:仅低温治疗、仅UVB照射、UVB照射后低温治疗,检测低温治疗对UVB照射后角质形成细胞的作用。将野生型雌性C57BL/6小鼠分为3组:低温治疗组、UVB组、UVB+低温治疗组。通过数字测微计测量、组织病理学、免疫组织化学、免疫荧光、定量RT-PCR和Western blot检测低温治疗对UVB诱导炎症的影响。结果低温治疗可显著减少UVB诱导的巨噬细胞和T细胞浸润(P<0.05),并显著抑制UVB诱导的p38磷酸化(P<0.05),减少相关的炎症反应,包括减轻耳肿胀反应、表皮增生(P<0.05)和皮肤炎症(P<0.05),减少小鼠模型中相关细胞因子表达和细胞凋亡(P<0.05)。结论低温治疗是治疗UVB诱导的皮肤炎症的一种有效的方法。 展开更多
关键词 低温治疗 炎症 p38 MAPK 中波紫外线
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Protective Effects of Jin Bai Mei Yan Prescription on Oxidative Damage and Photoaging Induced by Ultraviolet B in HaCaT Cells 被引量:2
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作者 NORIKO Minamisawa MIAO Ming-San +5 位作者 KANG Le LIU Hui-Juan CUI Lin-Lin HIROKI Kanemisu TERUMI Naito HIROAKI Shinotsuka 《Digital Chinese Medicine》 2020年第2期57-66,共10页
Objective Ultraviolet B(UVB)mainly acts on the skin epidermis,causing oxidative damage and apoptosis of keratinocytes.Jin Bai Mei Yan Prescription(JBMYP)comprises a variety of antioxidant traditional Chinese medicines... Objective Ultraviolet B(UVB)mainly acts on the skin epidermis,causing oxidative damage and apoptosis of keratinocytes.Jin Bai Mei Yan Prescription(JBMYP)comprises a variety of antioxidant traditional Chinese medicines(TCM).In this study,we aimed to evaluate the effects of JBMYP on the oxidative damage induced by UVB in human immortalized epidermal keratinocytes(HaCaT)cells.Methods HaCaT cells were divided into six groups:control group,model(UVB)group,positive(UVB+vitamin E)group,UVB+JBMYP low dose group(160μg/mL),UVB+JBMYP moderate dose group(800μg/mL),and UVB+JBMYP high dose group(1600μg/mL).HaCaT cells were irradiated with UVB and treated with JBMYP for 24 h.Methyl thiazolyl tetrazolium(MTT)assay and real-time unlabeled cell function analyzer were used to assess the cell survival and proliferation rates,respectively.At the same time,the levels of intracellular reactive oxygen species(ROS),lipid peroxide malondialdehyde(MDA),glutathione(GSH),and hydroxyproline(HYP),as well as the activities of antioxidant enzyme superoxide dismutase(SOD)and catalase(CAT)were evaluated using enzyme linked immunosorbent assay(ELISA).Results Compared with the model group,the survival rate of HaCaT cells in each dosage group of JBMYP was significantly improved(P<0.05).Further,JBMYP could promote the proliferation of HaCaT cells,leading to a reduction in the contents of MDA and ROS,and increase in the contents of SOD,CAT,GSH and HYP in HaCaT cells.Conclusions JBMYP has enhanced protective effect on oxidative damage induced by UVB in HaCaT cells. 展开更多
关键词 Jin bai Mei Yan Prescription(JbMYP) HaCaT cell Oxidative damage ultraviolet b(UVb) Chinese medicine ANTI-AGING
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Effects of Natural Radiation, PAR and Artificial Ultraviolet Radiation-B on the Ultrastructure and Histochemistry of Leaf of Oryza sativa L. 被引量:1
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作者 Sérgio L. de Almeida éder C. Schmidt +1 位作者 Ana C. Rodrigues Zenilda L. Bouzon 《American Journal of Plant Sciences》 2012年第10期1361-1368,共8页
Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivat... Ultraviolet radiation-B (UVBR) affects plants in many important ways, including reduced growth rate, reduction of primary productivity and changes in ultrastructure. The rice (Oryza sativa) is one of the most cultivated cereal in the world along with corn and wheat, representing over 50% of agricultural production. In this study, we examined O. sativa exposed to natural radiation denominated which “ambient samples”, plants cultivated which photosynthetically active radiation (PAR), denominated with PAR-only and plants cultivated with PAR + UVBR for 2 h per day during 30 days of cultivation in vitro. The samples were processed for electron microscopy and histochemistry analysis. PAR + UVBR caused changes in the ultrastructure of leaf of O. sativa, mesophyll cells, which included increased thickness of the cell wall and plastoglobuli, reduced intracellular spaces, changes in the cell contour, and destruction of chloroplast and mitochondria internal organization. The exposure to PAR + UVBR led to changes in guard and subsidiary cells, and the stomata and papillae were with irregular shape. The reduction of epicuticular wax that covered the leaf, was observed. Taken together, these ?ndings strongly suggested that PAR + UVBR negatively affects the ultrastructure and morphology and growth rates, of leaf of O. sativa and, in the long term, their economic viability. 展开更多
关键词 ORYZA SATIVA ultraviolet Radiation-b HISTOCHEMISTRY ULTRASTRUCTURE
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Review of narrowband ultraviolet B radiation in vitiligo
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作者 Enayat Attwa 《World Journal of Dermatology》 2016年第2期93-108,共16页
Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy u... Vitiligo is a common, acquired pigmentary disorder of unknown etiology with great impact on patient's appearance and quality of life. It presents a therapeutic challenge to many dermatologists. Photochemotherapy using psoralen and ultraviolet A(UVA) therapy, topical and oral immunosuppresants, as well as cosmetic camouflage are also commonly employed with varying clinical efficacy. Phototherapy is a popular treatment option, which includes both of the generalized ultraviolet B(UVB) therapies, broadband UVB and narrowband UVB(NB-UVB). It has been used favorably, both alone as well as in combination with other agents like topical calcineurin inhibitors, vitamin-D analogs. Combination therapies are useful and may provide quicker regimentation and treat vitiligo with an additive mechanism of action than UVB phototherapy. Advances in technology may lead to the continuing use of UVB phototherapy as a treatment for vitiligo through the development of sophisticated devices and delivery systems as well as innovative application methods. These will provide increased therapeutic options for all vitiligo patients, particularly those with refractory disease. In this article, I have reviewed the available data pertaining to efficacy and safety issues for NB-UVB as monotherapy, its comparison with psoralen plus UVA and other modes of phototherapy, combination regimens that have been tried and future prospects of NB-UVB in vitiligo. 展开更多
关键词 narrow-band ultraviolet b PHOTOTHERAPY VITILIGO
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Measurement and analysis of ozone, ultraviolet B and aerosol light scattering coefficients in the Arctic
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作者 LAI Xin BIAN Lingen +1 位作者 LU Changgui TANG Jie 《Advances in Polar Science》 2011年第4期260-265,共6页
Tropospheric ozone (O3), ultraviolet B (UVB) radiation and aerosol light scattering coefficients (SC) were investigated on a cruise ship during the fourth Chinese National Arctic Research Expedition from July 1 ... Tropospheric ozone (O3), ultraviolet B (UVB) radiation and aerosol light scattering coefficients (SC) were investigated on a cruise ship during the fourth Chinese National Arctic Research Expedition from July 1 September 20, 2010. The results showed that O3, UVB and SC decreased with increasing latitude, with minimum values recorded in the central Arctic Ocean. Average O3 concentrations were 15.9 ppbv and 15.1 ppbv in the Bering Sea and Arctic Ocean, respectively. Ozone concentrations increased to 17.5 ppbv in the high Arctic region. Average UVB values were 0.26 W.m-2 and 0.14 W.m-2 in the Bering Sea and Arctic Ocean, respectively. The average SC in the Bering Sea was 4.3 M.m-1, more than twice the value measured in the Arctic Ocean, which had an average value of 1.7 M.m-1. Overall, UVB and SC values were stable in the central Arctic Ocean. 展开更多
关键词 Arctic Ocean tropospheric ozone ultraviolet b radiation aerosol light scattering coefficient
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吡咯喹啉醌对UVB诱导的人皮肤成纤维细胞早衰的抑制作用
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作者 王静 施智男 +6 位作者 谷丽 翟小玉 徐智怡 赵静婷 顾丽群 花卉 周舒 《南通大学学报(医学版)》 2023年第3期224-229,共6页
目的:探究吡咯喹啉醌(pyrroloquinoline quinine,PQQ)对中波紫外线(ultraviolet B,UVB)诱导的人皮肤成纤维细胞早衰的影响。方法:采用不同浓度(10、30、50、60、100μmol/L)PQQ处理成纤维细胞24 h,使用CCK-8法检测细胞活力,筛选最适PQQ... 目的:探究吡咯喹啉醌(pyrroloquinoline quinine,PQQ)对中波紫外线(ultraviolet B,UVB)诱导的人皮肤成纤维细胞早衰的影响。方法:采用不同浓度(10、30、50、60、100μmol/L)PQQ处理成纤维细胞24 h,使用CCK-8法检测细胞活力,筛选最适PQQ浓度。将成纤维细胞分为对照组、PQQ组、光老化+PQQ组和光老化组。用UVB照射诱导人成纤维细胞提前衰老模型,后予50μmol/L PQQ处理24 h。采用流式细胞仪检测细胞周期;β-半乳糖苷酶(β-galacsidase,SA-β-Gal)染色观察细胞衰老;Western Blot法检测p16、p21、p53蛋白质在细胞内的表达情况;实时定量PCR(quantitative real-time PCR,qRT-PCR)法检测Ⅰ型胶原(typeⅠcollagen,COL-Ⅰ)、Ⅲ型胶原(typeⅢcollagen,COL-Ⅲ)、基质金属蛋白酶-1(matrix metalloproteinases-1,MMP-1)和基质金属蛋白酶-3(matrix metalloproteinases-3,MMP-3)mRNA表达水平。结果:与光老化组比较,光老化+PQQ组细胞形态改善,细胞间隙减小,SA-β-Gal阳性表达细胞减少,细胞增殖活性提高,细胞周期阻滞改善,p16、p21和p53蛋白表达减少,COL-Ⅰ、COL-ⅢmRNA表达量增加,MMP-1和MMP-3 mRNA表达降低。结论:PQQ对UVB诱导的人成纤维细胞提前衰老有抑制作用,其可能通过下调衰老相关蛋白p16、p21、p53的水平,调控MMP-1、MMP-3、COL-Ⅰ和COL-Ⅲ的基因表达而发挥光保护作用。 展开更多
关键词 成纤维细胞 皮肤 细胞早衰 吡咯喹啉醌 中波紫外线
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