Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determine...Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determined by immunohistochemical(IHC) staining using tissue microarrays with the H-score system and immunofluorescence(IF) staining of tumor cell lines.Imaging probes with the NIR fluorescent dye cyanine 7(Cy7) conjugated to COC183 B2 Mab were chemically engineered. OC183 B2-positive human OC cells(SKOV3-Luc) were injected subcutaneously into BALB/c nude mice. Bioluminescent imaging(BLI) was performed to detect tumor location and growth. COC183 B2-Cy7 at 1.1,3.3, 10, or 30 μg were used for in vivo fluorescence imaging, and phosphate-buffered saline(PBS), free Cy7 dye and mouse isotype immunoglobulin G(IgG)-Cy7(delivered at the same doses as COC183 B2-Cy7) were used as controls.Results: The expression of OC183 B2 with a high H-score was more prevalent in OC tissue than fallopian tube(FT) tissue. Among 417 OC patients, the expression of OC183 B2 was significantly correlated with the histological subtype, histological grade, residual tumor size, relapse state and survival status. IF staining demonstrated that COC183 B2 specifically expressed in SKOV3 cells but not HeLa cells. In vivo NIR fluorescence imaging indicated that COC183 B2-Cy7 was mainly distributed in the xenograft and liver with optimal tumor-to-background(T/B)ratios in the xenograft at 30 μg dose. The highest fluorescent signals in the tumor were observed at 96 h postinjection(hpi). Ex vivo fluorescence imaging revealed the fluorescent signals mainly from the tumor and liver. IHC analysis confirmed that xenografts were OC183 B2 positive.Conclusions: COC183 B2 is a good candidate for NIR fluorescence imaging and imaging-guided surgery in OC.展开更多
AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(...AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer.展开更多
BACKGROUND After an esophagectomy, the stomach is most commonly used to restore continuity of the upper gastrointestinal tract. These esophago-gastric anastomoses are prone to serious complications such as leakage ass...BACKGROUND After an esophagectomy, the stomach is most commonly used to restore continuity of the upper gastrointestinal tract. These esophago-gastric anastomoses are prone to serious complications such as leakage associated with high morbidity and mortality. Graft perfusion is considered to be an important predictor for anastomotic integrity. Based on the current literature we believe Indocyanine green fluorescence angiography(ICGA) is an easy assessment tool for gastric tube(GT) perfusion, and it might predict anastomotic leakage(AL).AIM To evaluate feasibility and effectiveness of ICGA in GT perfusion assessment and as a predictor of AL.METHODS This study was designed according to the PRISMA guidelines and registered in the PROSPERO database. PubMed and EMBASE were independently searched by 2 reviewers for studies presenting data on intraoperative ICGA GT perfusion assessment during esophago-gastric reconstruction after esophagectomy.Relevant outcomes such as feasibility, complications, intraoperative surgical changes based on ICGA findings, quantification attempts, anatomical data and the impact of ICGA on postoperative anastomotic complications, were collected by 2 independent researchers. The quality of the included articles was assessed based on the Methodological Index for Non-Randomized Studies. The 19 included studies presented data on 1192 esophagectomy patients, in 758 patients ICGA was used perioperative to guide esophageal reconstruction.RESULTS The 19 included studies for qualitative analyses all described ICGA as a safe and easy method to evaluate gastric graft perfusion. AL occurred in 13.8% of the entire cohort, 10% in the ICG guided group and 20.6% in the control group(P <0.001). When poorly perfused cases are excluded from the analyses, the difference in AL was even larger(AL well-perfused group 6.3% vs control group 20.5%, P <0.001). The AL rate in the group with an altered surgical plan based on the ICG image was 6.5%, similar to the well perfused group(6.3%) and significantly less than the poorly perfused group(47.8%)(P < 0.001), suggesting that the technique is able to identify and alter a potential bad outcome.CONCLUSION ICGA is a safe, feasible and promising method for perfusion assessment. The lower AL rate in the well perfused group suggest that a good fluorescent signal predicts a good outcome.展开更多
Objective: To develop a gold nanoparticles complex conjugated with interferon-gamma(IFN-g) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer cells.Method...Objective: To develop a gold nanoparticles complex conjugated with interferon-gamma(IFN-g) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer cells.Methods: Gold nanorods(10 nm) were conjugated with IFN-g and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT assy.Results: Zetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-g. The median percentage of cell viability in 0.30 mg/m L concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.60 mg/m L concentration of gold nanoparticles complex was toxic on tumor cells(P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to77% in 0.30 mg/m L concentration of gold nanorods complex.Conclusions: The size and concentration of gold nanorods was not cytotoxic. However,their presence during irradiation near-infrared laser increased the number of dead cells during the treatment of cells.展开更多
Sunlight that reaches the human skin contains solar energy composed of 6.8%ultraviolet(UV),38.9%visible light and 54.3%infrared radiation.In addition to natural near-infrared(NIR),human skin is increasingly exposed to...Sunlight that reaches the human skin contains solar energy composed of 6.8%ultraviolet(UV),38.9%visible light and 54.3%infrared radiation.In addition to natural near-infrared(NIR),human skin is increasingly exposed to artificial NIR from medical devices and electrical appliances.Thus,we are exposed to tremendous amounts of NIR.Many studies have proven the effects of UV exposure on human skin and skin cancers but have not investigated well the effects of NIR exposure.Furthermore,many of the previous NIR studies have used NIR resources without a water filter or a contact cooling.With these resources,a substantial amount of NIR energy is absorbed in the superficial layers and only limited NIR energy can be delivered to deeper tissues.Thus,they could not sufficiently evaluate the effects of incident solar NIR.In order to simulate solar NIR that reaches the skin,a water filter is essential because solar NIR is filtered by atmospheric water.In reality,NIR increases the surface temperature and induces thermal effects so a contact cooling is needed to pursue the properties of NIR.I clarify that NIR can penetrate the skin and non-thermally affect the subcutaneous tissues,including muscle and bone marrow,using a NIR resource with a water filter and a coolingsystem.I would like to emphasize the biological effects of NIR which have both merits and demerits.Appropriate NIR irradiation induces dermal heating thermally and non-thermally induces collagen and elastin stimulation,which results in skin tightening.NIR also induces non-thermal DNA damage of mitotic cells,which may have the potential application for treating cancer.However,as continuous NIR exposure may induce photoaging and potentially photocarcinogenesis,we should consider the effect of,not only UV,but also NIR and the necessity for protection against solar NIR.Here,this paper introduces the new aspects of the biological effects of NIR radiation.展开更多
目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例...目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例;年龄39~51岁,平均年龄45.23岁;病理类型,乳头状癌105例,滤泡性腺瘤17例;单侧切除术46例,双侧切除术76例。按照随机数表法将患者分为对照组和研究组,对照组患者行常规甲状腺癌手术,研究组患者在NIRAF技术辅助下行甲状腺切除术。比较两组患者甲状旁腺识别率、围术期指标、氧化应激指标、甲状腺功能、血钙、甲状旁腺激素(PTH)、免疫功能和并发症等参数。结果 研究组患者伤口长度、术中出血量、手术时间、术后引流量和住院时间与对照组比较,差异无统计学意义(P> 0.05)。两组患者均出现感染、发音困难和声音嘶哑等并发症,但差异无统计学意义(P> 0.05),而研究组患者术后低血钙和PTH减退比例明显低于对照组(22.95%vs 44.26%,6.56%vs 19.67%。P=0.013、0.032)。术后7 d两组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显提高,两组患者CD3^(+)CD8^(+)水平明显降低,且研究组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显高于对照组(43.83±4.59 vs 40.23±3.78,1.93±0.21 vs 1.73±0.13。P=0.000、0.000),研究组患者CD3^(+)CD8^(+)水平明显低于对照组(22.61±2.31 vs 23.81±2.62。P=0.008)。术后7 d两组患者血钙和PTH明显降低,且研究组患者血钙和PTH明显高于对照组(2.26±0.18 vs 2.15±0.25,36.98±5.16 vs 27.28±4.99。P=0.000、0.000)。研究组患者甲状腺被膜解剖前检出率为56.19%,明显高于对照组的31.19%(P=0.000);研究组患者甲状旁腺总检出率和总体准确度与对照组比较,差异无统计学意义(P> 0.05)。结论 NIRAF技术辅助识别甲状旁腺组织,可有效提高甲状腺被膜解剖前检出率,减小血钙和PTH减退幅度,增强术后甲状旁腺保护,改善免疫机能,值得临床进一步研究并推广。展开更多
基金supported by the National Key Research and Development Program of China (No.2016YFA0201400)National Natural Science Foundation of China (No. 81671431)
文摘Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determined by immunohistochemical(IHC) staining using tissue microarrays with the H-score system and immunofluorescence(IF) staining of tumor cell lines.Imaging probes with the NIR fluorescent dye cyanine 7(Cy7) conjugated to COC183 B2 Mab were chemically engineered. OC183 B2-positive human OC cells(SKOV3-Luc) were injected subcutaneously into BALB/c nude mice. Bioluminescent imaging(BLI) was performed to detect tumor location and growth. COC183 B2-Cy7 at 1.1,3.3, 10, or 30 μg were used for in vivo fluorescence imaging, and phosphate-buffered saline(PBS), free Cy7 dye and mouse isotype immunoglobulin G(IgG)-Cy7(delivered at the same doses as COC183 B2-Cy7) were used as controls.Results: The expression of OC183 B2 with a high H-score was more prevalent in OC tissue than fallopian tube(FT) tissue. Among 417 OC patients, the expression of OC183 B2 was significantly correlated with the histological subtype, histological grade, residual tumor size, relapse state and survival status. IF staining demonstrated that COC183 B2 specifically expressed in SKOV3 cells but not HeLa cells. In vivo NIR fluorescence imaging indicated that COC183 B2-Cy7 was mainly distributed in the xenograft and liver with optimal tumor-to-background(T/B)ratios in the xenograft at 30 μg dose. The highest fluorescent signals in the tumor were observed at 96 h postinjection(hpi). Ex vivo fluorescence imaging revealed the fluorescent signals mainly from the tumor and liver. IHC analysis confirmed that xenografts were OC183 B2 positive.Conclusions: COC183 B2 is a good candidate for NIR fluorescence imaging and imaging-guided surgery in OC.
基金Supported by a Grant-in-Aid for Scientific Research(C)from the Ministry of Education,Culture,Sports,Science,and Technology,Japan,No.17K10460(to Aung W)
文摘AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer.
基金"Kom op tegen Kanker"(Stand up to Cancer),the Flemish cancer society
文摘BACKGROUND After an esophagectomy, the stomach is most commonly used to restore continuity of the upper gastrointestinal tract. These esophago-gastric anastomoses are prone to serious complications such as leakage associated with high morbidity and mortality. Graft perfusion is considered to be an important predictor for anastomotic integrity. Based on the current literature we believe Indocyanine green fluorescence angiography(ICGA) is an easy assessment tool for gastric tube(GT) perfusion, and it might predict anastomotic leakage(AL).AIM To evaluate feasibility and effectiveness of ICGA in GT perfusion assessment and as a predictor of AL.METHODS This study was designed according to the PRISMA guidelines and registered in the PROSPERO database. PubMed and EMBASE were independently searched by 2 reviewers for studies presenting data on intraoperative ICGA GT perfusion assessment during esophago-gastric reconstruction after esophagectomy.Relevant outcomes such as feasibility, complications, intraoperative surgical changes based on ICGA findings, quantification attempts, anatomical data and the impact of ICGA on postoperative anastomotic complications, were collected by 2 independent researchers. The quality of the included articles was assessed based on the Methodological Index for Non-Randomized Studies. The 19 included studies presented data on 1192 esophagectomy patients, in 758 patients ICGA was used perioperative to guide esophageal reconstruction.RESULTS The 19 included studies for qualitative analyses all described ICGA as a safe and easy method to evaluate gastric graft perfusion. AL occurred in 13.8% of the entire cohort, 10% in the ICG guided group and 20.6% in the control group(P <0.001). When poorly perfused cases are excluded from the analyses, the difference in AL was even larger(AL well-perfused group 6.3% vs control group 20.5%, P <0.001). The AL rate in the group with an altered surgical plan based on the ICG image was 6.5%, similar to the well perfused group(6.3%) and significantly less than the poorly perfused group(47.8%)(P < 0.001), suggesting that the technique is able to identify and alter a potential bad outcome.CONCLUSION ICGA is a safe, feasible and promising method for perfusion assessment. The lower AL rate in the well perfused group suggest that a good fluorescent signal predicts a good outcome.
基金Supported by a grant from Iran National Science Foundation under the grant number 91002993
文摘Objective: To develop a gold nanoparticles complex conjugated with interferon-gamma(IFN-g) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer cells.Methods: Gold nanorods(10 nm) were conjugated with IFN-g and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT assy.Results: Zetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-g. The median percentage of cell viability in 0.30 mg/m L concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.60 mg/m L concentration of gold nanoparticles complex was toxic on tumor cells(P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to77% in 0.30 mg/m L concentration of gold nanorods complex.Conclusions: The size and concentration of gold nanorods was not cytotoxic. However,their presence during irradiation near-infrared laser increased the number of dead cells during the treatment of cells.
文摘Sunlight that reaches the human skin contains solar energy composed of 6.8%ultraviolet(UV),38.9%visible light and 54.3%infrared radiation.In addition to natural near-infrared(NIR),human skin is increasingly exposed to artificial NIR from medical devices and electrical appliances.Thus,we are exposed to tremendous amounts of NIR.Many studies have proven the effects of UV exposure on human skin and skin cancers but have not investigated well the effects of NIR exposure.Furthermore,many of the previous NIR studies have used NIR resources without a water filter or a contact cooling.With these resources,a substantial amount of NIR energy is absorbed in the superficial layers and only limited NIR energy can be delivered to deeper tissues.Thus,they could not sufficiently evaluate the effects of incident solar NIR.In order to simulate solar NIR that reaches the skin,a water filter is essential because solar NIR is filtered by atmospheric water.In reality,NIR increases the surface temperature and induces thermal effects so a contact cooling is needed to pursue the properties of NIR.I clarify that NIR can penetrate the skin and non-thermally affect the subcutaneous tissues,including muscle and bone marrow,using a NIR resource with a water filter and a coolingsystem.I would like to emphasize the biological effects of NIR which have both merits and demerits.Appropriate NIR irradiation induces dermal heating thermally and non-thermally induces collagen and elastin stimulation,which results in skin tightening.NIR also induces non-thermal DNA damage of mitotic cells,which may have the potential application for treating cancer.However,as continuous NIR exposure may induce photoaging and potentially photocarcinogenesis,we should consider the effect of,not only UV,but also NIR and the necessity for protection against solar NIR.Here,this paper introduces the new aspects of the biological effects of NIR radiation.
文摘目的 探讨近红外自体荧光显像(NIRAF)技术辅助识别甲状旁腺的有效性及对术后甲状旁腺保护和甲状旁腺激素表达的影响。方法 选择2020年1月至2022年9月在邢台医学高等专科学校第二附属医院收治的122例甲状腺癌患者,其中男性95例,女性27例;年龄39~51岁,平均年龄45.23岁;病理类型,乳头状癌105例,滤泡性腺瘤17例;单侧切除术46例,双侧切除术76例。按照随机数表法将患者分为对照组和研究组,对照组患者行常规甲状腺癌手术,研究组患者在NIRAF技术辅助下行甲状腺切除术。比较两组患者甲状旁腺识别率、围术期指标、氧化应激指标、甲状腺功能、血钙、甲状旁腺激素(PTH)、免疫功能和并发症等参数。结果 研究组患者伤口长度、术中出血量、手术时间、术后引流量和住院时间与对照组比较,差异无统计学意义(P> 0.05)。两组患者均出现感染、发音困难和声音嘶哑等并发症,但差异无统计学意义(P> 0.05),而研究组患者术后低血钙和PTH减退比例明显低于对照组(22.95%vs 44.26%,6.56%vs 19.67%。P=0.013、0.032)。术后7 d两组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显提高,两组患者CD3^(+)CD8^(+)水平明显降低,且研究组患者CD3^(+)CD4^(+)和CD4^(+)/CD8^(+)水平明显高于对照组(43.83±4.59 vs 40.23±3.78,1.93±0.21 vs 1.73±0.13。P=0.000、0.000),研究组患者CD3^(+)CD8^(+)水平明显低于对照组(22.61±2.31 vs 23.81±2.62。P=0.008)。术后7 d两组患者血钙和PTH明显降低,且研究组患者血钙和PTH明显高于对照组(2.26±0.18 vs 2.15±0.25,36.98±5.16 vs 27.28±4.99。P=0.000、0.000)。研究组患者甲状腺被膜解剖前检出率为56.19%,明显高于对照组的31.19%(P=0.000);研究组患者甲状旁腺总检出率和总体准确度与对照组比较,差异无统计学意义(P> 0.05)。结论 NIRAF技术辅助识别甲状旁腺组织,可有效提高甲状腺被膜解剖前检出率,减小血钙和PTH减退幅度,增强术后甲状旁腺保护,改善免疫机能,值得临床进一步研究并推广。
