To estimate the near-fault inelastic response spectra, the accuracy of six existing strength reduction factors (R) proposed by different investigators were evaluated by using a suite of near-fault earthquake records...To estimate the near-fault inelastic response spectra, the accuracy of six existing strength reduction factors (R) proposed by different investigators were evaluated by using a suite of near-fault earthquake records with directivity-induced pulses. In the evaluation, the force-deformation relationship is modelled by elastic-perfectly plastic, bilinear and stiffness degrading models, and two site conditions, rock and soil, are considered. The R-value ratio (ratio of the R value obtained from the existing R-expressions (or the R-p-T relationships) to that from inelastic analyses) is used as a measurement parameter. Results show that the R-expressions proposed by Ordaz & Perez-Rocha are the most suitable for near-fault ground motions, followed by the Newmark & Hall and the Berrill et al. relationships. Based on an analysis using the near-fault ground motion dataset, new expressions of R that consider the effects of site conditions are presented and verified.展开更多
AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(...AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer.展开更多
The relationship between the factor of temperature difference of the near-surface layer(T_(1000 hPa)-T_(2m))and sea fog is analyzed using the NCEP reanalysis with a horizontal resolution of l°xl°(2000 to 201...The relationship between the factor of temperature difference of the near-surface layer(T_(1000 hPa)-T_(2m))and sea fog is analyzed using the NCEP reanalysis with a horizontal resolution of l°xl°(2000 to 2011) and the station observations(2010 to 2011).The element is treated as the prediction variable factor in the GRAPES model and used to improve the regional prediction of sea fog on Guangdong coastland.(1) The relationship between this factor and the occurrence of sea fog is explicit:When the sea fog happens,the value of this factor is always large in some specific periods,and the negative value of this factor decreases significantly or turns positive,suggesting the enhancement of warm and moist advection of air flow near the surface,which favors the development of sea fog.(2) The transportation of warm and moist advection over Guangdong coastland is featured by some stages and the jumping among these states.It also gets stronger over time.Meanwhile,the northward propagation of warm and moist advection is quite consistent with the northward advancing of sea fog from south to north along the coastland of China.(3) The GRAPES model can well simulate and realize the factor of near-surface temperature difference.Besides,the accuracy of regional prediction of marine fog,the relevant threat score and Heidke skill score are all improved when the factor is involved.展开更多
Nonlinear response history analyses and use of strong ground motion data including near-field effects has become a common practice in both performance based design of tall buildings and design of base-isolated buildin...Nonlinear response history analyses and use of strong ground motion data including near-field effects has become a common practice in both performance based design of tall buildings and design of base-isolated buildings. On the other hand, ordinary buildings are commonly analysed via response spectrum analysis following the rules of conventional seismic codes, most of which do not take near-field effects into account. This study evaluates the necessity and the adequacy of near-source factors for ordinary fixed-base buildings that are not specifically classified as tall, by comparing dynamic responses of 3, 8, and 15-story benchmark buildings obtained via (1) linear time history analyses using 220 record components from 13 historical earthquakes and 45 synthetic earthquake records of different magnitudes and fault distances and (2) response spectrum analyses in accordance with the Turkish Earthquake Code 2007 -representing seismic codes not taking near-field effects into account- and the Uniform Building Code 1997 which takes near-field effects into account via near-source factors that amplify design response spectrum. It is shown that near-source factors are crucial for the safe design of not-so-tall ordinary fixed-base buildings but those defined in UBC97 may still not be adequate for those located in the vicinity of the fault.展开更多
Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground ...Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground motion is adopted to study the characteristics of near-fault ground motion. For both slip models, near-fault strong ground motion with high amplitude is located in a narrow belt area along the projection of the fault on the ground and mainly controlled by the sub-faults nearby. Directivity of strike-slip fault is more dominant in long period for components perpendicular to the fault, and more dominant in long period for components parallel to the fault for dip-slip fault. The deeper the location of the source is, the more slowly the amplitude of ground motion attenuates. There is obvious hanging wall effect in ground motion of inclined fault, and the spatial distribution of ground motion is asymmetric which coincides with observational data. Finally, a fitting function of spatial distribution for near-fault ground motion is proposed and compared with near source factors of the 1997 Uniform Building Code of USA.展开更多
Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground ...Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground motion is adopted to study the characteristics of near-fault ground motion. For both slip models, near-fault strong ground motion with high amplitude is located in a narrow belt area along the projection of the fault on the ground and mainly controlled by the sub-faults nearby. Directivity of strike-slip fault is more dominant in long period for components perpendicular to the fault, and more dominant in long period for components parallel to the fault for dip-slip fault. The deeper the location of the source is, the more slowly the amplitude of ground motion attenuates. There is obvious hanging wall effect in ground motion of inclined fault, and the spatial distribution of ground motion is asymmetric which coincides with observational data. Finally, a fitting function of spatial distribution for near-fault ground motion is proposed and compared with near source factors of the 1997 Uniform Building Code of USA.展开更多
Soil organic matter(SOM) is an important parameter related to soil nutrient and miscellaneous ecosystem services. This paper attempts to improve the performance of traditional partial least square regression(PLSR) mod...Soil organic matter(SOM) is an important parameter related to soil nutrient and miscellaneous ecosystem services. This paper attempts to improve the performance of traditional partial least square regression(PLSR) model by considering the spatial autocorrelation and soil forming factors. Surface soil samples(n = 180) were collected from Honghu City located in the middle of Jianghan Plain, China. The visible and near infrared(VNIR) spectra and six environmental factors(elevation, land use types, roughness, relief amplitude, enhanced vegetation index, and land surface water index) were used as the auxiliary variables to construct the multiple linear regression(MLR), PLSR and geographically weighted regression(GWR) models. Results showed that: 1) the VNIR spectra can increase about 39.62% prediction accuracy than the environmental factors in predicting SOM; 2) the comprehensive variables of VNIR spectra and the environmental factors can improve about 5.78% and 44.90% relative to soil spectral models and soil environmental models, respectively; 3) the spatial model(GWR) can improve about 3.28% accuracy than MLR and PLSR. Our results suggest that the combination of spectral reflectance and the environmental variables can be used as the suitable auxiliary variables in predicting SOM, and GWR is a promising model for predicting soil properties.展开更多
It was shown from the study on the recently near-fault earthquake ground motions that the near-fault effects were seldom considered in the existing Chinese seismic code. Referring to the UBC97 design concept for near-...It was shown from the study on the recently near-fault earthquake ground motions that the near-fault effects were seldom considered in the existing Chinese seismic code. Referring to the UBC97 design concept for near-fault factors, based on the collected world-widely free-site records of near-fault earthquakes ground motions classified by earthquake magnitude and site condition, the attenuation relationship expressions of the acceleration spectrum demand at the key points within the long period and moderate period were established in term of the earthquake magnitude and the site condition. Furthermore, the near-fault factors' expressions about the earthquake magnitude and the fault distance were deduced for the area lack of near-fault strong earthquake records. Based on the current Chinese Building Seismic Design Code, the near-fault effect factors and the modified design spectral curves, which were valuable for the seismic design, were proposed to analyze the seismic response of structures.展开更多
The purpose of this study was to determine the effects of skin pigmentation regarding Near Infrared Spectroscopy (NIRS) tissue oxygen saturation values (StO2). The study examined NIRS values in individuals with varyin...The purpose of this study was to determine the effects of skin pigmentation regarding Near Infrared Spectroscopy (NIRS) tissue oxygen saturation values (StO2). The study examined NIRS values in individuals with varying skin pigmentation on the anterior compartment of the lower leg and volar forearm to determine if correlation exists among three NIRS devices, the EQUANOX, Casmed, and INVOS. Skin pigmentation was measured on the anterior lower leg (AL) and volar forearm (VF) of participants using a noninvasive colorimeter that employed reflective spectroscopy to produce a quantitative value for erythema (skin “redness”) and melanin (skin pigment). Muscle oxygenation was measured using three oximetry devices with sensors placed in the same areas. The EQUANOX device showed no significant correlation with skin pigmentation, while the Casmed and INVOS devices showed moderate and significant correlation with skin pigmentation, respectively. Different devices have different abilities to remove confounding variables, such as skin pigmentation and erythema, which may affect clinical decision-making, and affect the use of NIRS technology.展开更多
Objective: To investigate the effect of fluorescent dye labeling on the targeting capabilities of 111 In- (DTPA).-trastuzumab-(IRDye 800)m. Methods: Trastuzumab-based conjugates were synthesized and conjugated w...Objective: To investigate the effect of fluorescent dye labeling on the targeting capabilities of 111 In- (DTPA).-trastuzumab-(IRDye 800)m. Methods: Trastuzumab-based conjugates were synthesized and conjugated with diethylenetriaminepentaacetic acid (DTPA) at molar ratios of I, 2, 3 and 5 and with a fluorescent dye (IRDye 800CW) at molar ratios of 1, 3 and 5. Immunoreactivity and internalization were assessed on SKBR-3 cells, overexpressing human epidermal growth factor receptor 2. The stability in human serum and phosphate-buffered saline (PBS) was evaluated. The biodistribution of dual-labeled conjugates was compared with that of 111In-(DTPA)2-trastuzumab in a SKBR-3 xenograft model to evaluate the effect of dye-to-protein ratio. Results: All trastuzumab-based conjugates exhibited a high level of chemical and optical purity. Flow cytometry results showed that increasing dye-to-protein ratios were associated with decreased immunoreactivity. Stability studies revealed that the conjugate was stable in PBS, while in human serum, increased degradation and protein precipitation were observed with increasing dye-to-protein ratios. At 4 h, the percentages of internalization of dual-labeled conjugates normalized by dye-to-protein ratio (m) were 24.88%±2.10%, 19.99%±0.59%, and 17.47%±1.26% for "m" equal to 1, 3, and 5, respectively. A biodistribution study revealed a progressive decrease in tumor uptake with an increase in the dye-to-protein ratios. The liver, spleen and kidney showed a marked uptake with increased dye-to-protein ratios, particularly in the latter. Conclusions: With non-specific-site conjugation of the fluorescent dye with a protein based on imaging agent, the increase in dye-to-protein ratios negatively impacted the immunoreactivity and stability, indicating a reduced tumor uptake.展开更多
Depression has been known to reduce the prefrontal activity associated with the execution of certain cognitive tasks, although whether a temporarily depressed or anxious mood in healthy individuals affects the prefron...Depression has been known to reduce the prefrontal activity associated with the execution of certain cognitive tasks, although whether a temporarily depressed or anxious mood in healthy individuals affects the prefrontal blood oxygen level during cognitive tasks is unknown. Combining the measurement of prefrontal activity with near-infrared spectroscopy (NIRS) and the two cognitive tasks, namely the letter version of the verbal fluency test (VFT-l) and the Stroop test, we measured the effect of a depressed or anxious mood and gender on the changes in the prefrontal oxygenated hemoglobin (Oxy-Hb) levels during those cognitive tests in healthy individuals. Depressed mood or anxious mood was assessed by the Hospital Anxiety and Depression Scale (HADS). Thereby we aimed to explore the possibility of NIRS measurement for detecting the early subclinical manifestation of major depression. Moreover, we examined the possible relationships between prefrontal activation and the functional Val66Met polymorphisms of the brain derived neurotropic factor (BDNF) gene and serum BDNF level. As a result, the increased prefrontal Oxy-Hb levels during cognitive tasks were significantly correlated with the severity of depressed mood in males. The course of the prefrontal Oxy-Hb increase was different depending on the cognitive tasks, i.e., the VFT-l or the Stroop test, in both genders. Correlations of BDNF genotype and serum BDNF level with the prefrontal Oxy-Hb levels during those cognitive tasks were negative. Our results suggest that the early subclinical manifestation of depressed mood in males might be detected by the NIRS measurement, which is not correlated with the individual properties of BDNF.展开更多
基金Foundation for Research and Science and Technology of New Zealand, Contract Number: C05X0208 and C05X0301the Foundation for Western Transportation Science and Technology Research, Contract No. 200831800098
文摘To estimate the near-fault inelastic response spectra, the accuracy of six existing strength reduction factors (R) proposed by different investigators were evaluated by using a suite of near-fault earthquake records with directivity-induced pulses. In the evaluation, the force-deformation relationship is modelled by elastic-perfectly plastic, bilinear and stiffness degrading models, and two site conditions, rock and soil, are considered. The R-value ratio (ratio of the R value obtained from the existing R-expressions (or the R-p-T relationships) to that from inelastic analyses) is used as a measurement parameter. Results show that the R-expressions proposed by Ordaz & Perez-Rocha are the most suitable for near-fault ground motions, followed by the Newmark & Hall and the Berrill et al. relationships. Based on an analysis using the near-fault ground motion dataset, new expressions of R that consider the effects of site conditions are presented and verified.
基金Supported by a Grant-in-Aid for Scientific Research(C)from the Ministry of Education,Culture,Sports,Science,and Technology,Japan,No.17K10460(to Aung W)
文摘AIM To investigate near-infrared photoimmunotherapeutic effect mediated by an anti-tissue factor(TF) antibody conjugated to indocyanine green(ICG) in a pancreatic cancer model.METHODS Near-infrared photoimmunotherapy(NIR-PIT) is a highly selective tumor treatment that utilizes an antibody-photosensitizer conjugate administration, followed by NIR light exposure. Anti-TF antibody 1849-ICG conjugate was synthesized by labeling of rat IgG2 b anti-TF monoclonal antibody 1849(anti-TF 1849) to a NIR photosensitizer,ICG. The expression levels of TF in two human pancreatic cancer cell lines were examined by western blotting. Specific binding of the 1849-ICG to TF-expressing BxPC-3 cells was examined by fluorescence microscopy. NIR-PITinduced cell death was determined by cell viability imaging assay. In vivo longitudinal fluorescence imaging was used to explore the accumulation of 1849-ICG conjugate in xenograft tumors. To examine the effect of NIRPIT, tumor-bearing mice were separated into 5 groups:(1) 100 μg of 1849-ICG i.v. administration followed by NIR light exposure(50 J/cm2) on two consecutive days(Days 1 and 2);(2) NIR light exposure(50 J/cm2) only on two consecutive days(Days 1 and 2);(3) 100 μg of 1849-ICG i.v. administration;(4) 100 μg of unlabeled antiTF 1849 i.v. administration; and(5) the untreated control. Semiweekly tumor volume measurements, accompanied with histological and immunohistochemical(IHC) analyses of tumors, were performed 3 d after the 2nd irradiation with NIR light to monitor the effect of treatments. RESULTS High TF expression in BxPC-3 cells was observed via western blot analysis, concordant with the observed preferential binding with intracellular localization of 1849-ICG via fluorescence microscopy. NIR-PIT-induced cell death was observed by performing cell viability imaging assay. In contrast to the other test groups, tumor growth was significantly inhibited by NIR-PIT with a statistically significant difference in relative tumor volumes for 27 d after the treatment start date [2.83 ± 0.38(NIR-PIT) vs 5.42 ± 1.61(Untreated), vs 4.90 ± 0.87(NIR), vs 4.28 ±1.87(1849-ICG), vs 4.35 ± 1.42(anti-TF 1849), at Day 27, P < 0.05]. Tumors that received NIR-PIT showed evidence of necrotic cell death-associated features upon hematoxylin-eosin staining accompanied by a decrease in Ki-67-positive cells(a cell proliferation marker) by IHC examination.CONCLUSION The TF-targeted NIR-PIT with the 1849-ICG conjugate can potentially open a new platform for treatment of TF-expressing pancreatic cancer.
基金Chinese Special Scientific Research Project for Public Interest(GYHY200906008)Natural Science Foundation of China(41275025)+2 种基金Guangdong Science and Technology Plan Project(2012A061400012)Meteorological Project from Guangdong Meteorological Bureau(201003)Research on Pre-warning and Forecasting Techniques for Marine Meteorology from Guangdong Meteorological Bureau
文摘The relationship between the factor of temperature difference of the near-surface layer(T_(1000 hPa)-T_(2m))and sea fog is analyzed using the NCEP reanalysis with a horizontal resolution of l°xl°(2000 to 2011) and the station observations(2010 to 2011).The element is treated as the prediction variable factor in the GRAPES model and used to improve the regional prediction of sea fog on Guangdong coastland.(1) The relationship between this factor and the occurrence of sea fog is explicit:When the sea fog happens,the value of this factor is always large in some specific periods,and the negative value of this factor decreases significantly or turns positive,suggesting the enhancement of warm and moist advection of air flow near the surface,which favors the development of sea fog.(2) The transportation of warm and moist advection over Guangdong coastland is featured by some stages and the jumping among these states.It also gets stronger over time.Meanwhile,the northward propagation of warm and moist advection is quite consistent with the northward advancing of sea fog from south to north along the coastland of China.(3) The GRAPES model can well simulate and realize the factor of near-surface temperature difference.Besides,the accuracy of regional prediction of marine fog,the relevant threat score and Heidke skill score are all improved when the factor is involved.
文摘Nonlinear response history analyses and use of strong ground motion data including near-field effects has become a common practice in both performance based design of tall buildings and design of base-isolated buildings. On the other hand, ordinary buildings are commonly analysed via response spectrum analysis following the rules of conventional seismic codes, most of which do not take near-field effects into account. This study evaluates the necessity and the adequacy of near-source factors for ordinary fixed-base buildings that are not specifically classified as tall, by comparing dynamic responses of 3, 8, and 15-story benchmark buildings obtained via (1) linear time history analyses using 220 record components from 13 historical earthquakes and 45 synthetic earthquake records of different magnitudes and fault distances and (2) response spectrum analyses in accordance with the Turkish Earthquake Code 2007 -representing seismic codes not taking near-field effects into account- and the Uniform Building Code 1997 which takes near-field effects into account via near-source factors that amplify design response spectrum. It is shown that near-source factors are crucial for the safe design of not-so-tall ordinary fixed-base buildings but those defined in UBC97 may still not be adequate for those located in the vicinity of the fault.
基金National Natural Science Foundation of China (59895410) Commonweal Foundation of the Ministry of Science and Technology of China (2001DIB20098).
文摘Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground motion is adopted to study the characteristics of near-fault ground motion. For both slip models, near-fault strong ground motion with high amplitude is located in a narrow belt area along the projection of the fault on the ground and mainly controlled by the sub-faults nearby. Directivity of strike-slip fault is more dominant in long period for components perpendicular to the fault, and more dominant in long period for components parallel to the fault for dip-slip fault. The deeper the location of the source is, the more slowly the amplitude of ground motion attenuates. There is obvious hanging wall effect in ground motion of inclined fault, and the spatial distribution of ground motion is asymmetric which coincides with observational data. Finally, a fitting function of spatial distribution for near-fault ground motion is proposed and compared with near source factors of the 1997 Uniform Building Code of USA.
基金National Natural Science Foundation of China (59895410), Commonweal Foundation of the Ministry of Science and Technology of China (2001DIB20098).
文摘Near-fault strong ground motion of strike-slip and dip-slip of vertical and inclined rectangular fault in half-space and layered half-space is analyzed by dislocation source model. The Fourier spectra ratio of ground motion is adopted to study the characteristics of near-fault ground motion. For both slip models, near-fault strong ground motion with high amplitude is located in a narrow belt area along the projection of the fault on the ground and mainly controlled by the sub-faults nearby. Directivity of strike-slip fault is more dominant in long period for components perpendicular to the fault, and more dominant in long period for components parallel to the fault for dip-slip fault. The deeper the location of the source is, the more slowly the amplitude of ground motion attenuates. There is obvious hanging wall effect in ground motion of inclined fault, and the spatial distribution of ground motion is asymmetric which coincides with observational data. Finally, a fitting function of spatial distribution for near-fault ground motion is proposed and compared with near source factors of the 1997 Uniform Building Code of USA.
基金Under the auspices of the Natural Science Foundation of Hubei(No.2018CFB372)the Fundamental Research Funds for the Central Universities(No.2662016QD032)+2 种基金the Key Laboratory of Aquatic Plants and Watershed Ecology of Chinese Academy of Sciences(No.Y852721s04)the Chinese National Natural Science Foundation(No.41371227)the National Undergraduate Innovation and Entrepreneurship Training Program(No.201810504023,201810504030)
文摘Soil organic matter(SOM) is an important parameter related to soil nutrient and miscellaneous ecosystem services. This paper attempts to improve the performance of traditional partial least square regression(PLSR) model by considering the spatial autocorrelation and soil forming factors. Surface soil samples(n = 180) were collected from Honghu City located in the middle of Jianghan Plain, China. The visible and near infrared(VNIR) spectra and six environmental factors(elevation, land use types, roughness, relief amplitude, enhanced vegetation index, and land surface water index) were used as the auxiliary variables to construct the multiple linear regression(MLR), PLSR and geographically weighted regression(GWR) models. Results showed that: 1) the VNIR spectra can increase about 39.62% prediction accuracy than the environmental factors in predicting SOM; 2) the comprehensive variables of VNIR spectra and the environmental factors can improve about 5.78% and 44.90% relative to soil spectral models and soil environmental models, respectively; 3) the spatial model(GWR) can improve about 3.28% accuracy than MLR and PLSR. Our results suggest that the combination of spectral reflectance and the environmental variables can be used as the suitable auxiliary variables in predicting SOM, and GWR is a promising model for predicting soil properties.
基金National Natural Science Foundation of China (50278002)Dalian Nationalities University Ph D Foundation (20066104)
文摘It was shown from the study on the recently near-fault earthquake ground motions that the near-fault effects were seldom considered in the existing Chinese seismic code. Referring to the UBC97 design concept for near-fault factors, based on the collected world-widely free-site records of near-fault earthquakes ground motions classified by earthquake magnitude and site condition, the attenuation relationship expressions of the acceleration spectrum demand at the key points within the long period and moderate period were established in term of the earthquake magnitude and the site condition. Furthermore, the near-fault factors' expressions about the earthquake magnitude and the fault distance were deduced for the area lack of near-fault strong earthquake records. Based on the current Chinese Building Seismic Design Code, the near-fault effect factors and the modified design spectral curves, which were valuable for the seismic design, were proposed to analyze the seismic response of structures.
文摘The purpose of this study was to determine the effects of skin pigmentation regarding Near Infrared Spectroscopy (NIRS) tissue oxygen saturation values (StO2). The study examined NIRS values in individuals with varying skin pigmentation on the anterior compartment of the lower leg and volar forearm to determine if correlation exists among three NIRS devices, the EQUANOX, Casmed, and INVOS. Skin pigmentation was measured on the anterior lower leg (AL) and volar forearm (VF) of participants using a noninvasive colorimeter that employed reflective spectroscopy to produce a quantitative value for erythema (skin “redness”) and melanin (skin pigment). Muscle oxygenation was measured using three oximetry devices with sensors placed in the same areas. The EQUANOX device showed no significant correlation with skin pigmentation, while the Casmed and INVOS devices showed moderate and significant correlation with skin pigmentation, respectively. Different devices have different abilities to remove confounding variables, such as skin pigmentation and erythema, which may affect clinical decision-making, and affect the use of NIRS technology.
基金supported by Beijing Natural Science Foundation(No.7132037)the National Cancer Institute Network for Translational Research U54 CA136404-01
文摘Objective: To investigate the effect of fluorescent dye labeling on the targeting capabilities of 111 In- (DTPA).-trastuzumab-(IRDye 800)m. Methods: Trastuzumab-based conjugates were synthesized and conjugated with diethylenetriaminepentaacetic acid (DTPA) at molar ratios of I, 2, 3 and 5 and with a fluorescent dye (IRDye 800CW) at molar ratios of 1, 3 and 5. Immunoreactivity and internalization were assessed on SKBR-3 cells, overexpressing human epidermal growth factor receptor 2. The stability in human serum and phosphate-buffered saline (PBS) was evaluated. The biodistribution of dual-labeled conjugates was compared with that of 111In-(DTPA)2-trastuzumab in a SKBR-3 xenograft model to evaluate the effect of dye-to-protein ratio. Results: All trastuzumab-based conjugates exhibited a high level of chemical and optical purity. Flow cytometry results showed that increasing dye-to-protein ratios were associated with decreased immunoreactivity. Stability studies revealed that the conjugate was stable in PBS, while in human serum, increased degradation and protein precipitation were observed with increasing dye-to-protein ratios. At 4 h, the percentages of internalization of dual-labeled conjugates normalized by dye-to-protein ratio (m) were 24.88%±2.10%, 19.99%±0.59%, and 17.47%±1.26% for "m" equal to 1, 3, and 5, respectively. A biodistribution study revealed a progressive decrease in tumor uptake with an increase in the dye-to-protein ratios. The liver, spleen and kidney showed a marked uptake with increased dye-to-protein ratios, particularly in the latter. Conclusions: With non-specific-site conjugation of the fluorescent dye with a protein based on imaging agent, the increase in dye-to-protein ratios negatively impacted the immunoreactivity and stability, indicating a reduced tumor uptake.
文摘Depression has been known to reduce the prefrontal activity associated with the execution of certain cognitive tasks, although whether a temporarily depressed or anxious mood in healthy individuals affects the prefrontal blood oxygen level during cognitive tasks is unknown. Combining the measurement of prefrontal activity with near-infrared spectroscopy (NIRS) and the two cognitive tasks, namely the letter version of the verbal fluency test (VFT-l) and the Stroop test, we measured the effect of a depressed or anxious mood and gender on the changes in the prefrontal oxygenated hemoglobin (Oxy-Hb) levels during those cognitive tests in healthy individuals. Depressed mood or anxious mood was assessed by the Hospital Anxiety and Depression Scale (HADS). Thereby we aimed to explore the possibility of NIRS measurement for detecting the early subclinical manifestation of major depression. Moreover, we examined the possible relationships between prefrontal activation and the functional Val66Met polymorphisms of the brain derived neurotropic factor (BDNF) gene and serum BDNF level. As a result, the increased prefrontal Oxy-Hb levels during cognitive tasks were significantly correlated with the severity of depressed mood in males. The course of the prefrontal Oxy-Hb increase was different depending on the cognitive tasks, i.e., the VFT-l or the Stroop test, in both genders. Correlations of BDNF genotype and serum BDNF level with the prefrontal Oxy-Hb levels during those cognitive tasks were negative. Our results suggest that the early subclinical manifestation of depressed mood in males might be detected by the NIRS measurement, which is not correlated with the individual properties of BDNF.