Cloning of sft-4 and its influence on vitality and virulence of pine wood nematode,Bursaphelenchus xylophilus

In our previous screening of the transcriptome of the causal agent of the devastating pine wilt disease,pine wood nematode(PWN,Bursaphelenchus xylophilus),after treatment with the nematicide fomepizole,Surfeit locus gene sft-4,which encodes a regulatory factor,was found to be downregulated.In situ hybridization results showed that the sft-4 was continuously expressed from egg to adult and was especially high in the reproductive system.Here in a study of the effect of RNA interference(RNAi)of sft-4 and recombinant SFT-4 on PWN activity,treatment with sft-4 dsRNA inhibited feeding,reproduction,oviposition and egg hatching of PWN with the greatest inhibition on reproduction and oviposition,whereas recombinant SFT-4 had the opposite effect.In addition,RNAi of sft-4 changed the female–male ratio and lifespan of PWN.In bioassays of PWNs,with RNAi of sft-4 on seedlings and 2-year-old Pinus thunbergii trees,none of the treated plants developed symp-toms during the monitoring period,indicating that virulence of PWNs was either significantly weakened.These results indicate that the influence of sft-4 on PWN pathogenicity may be mainly through regulating reproductive function of PWN and its lifespan.

Modeling the effect of stand and site characteristics on the probability of mistletoe infestation in Scots pine stands using remote sensing data

Over the past decade,the presence of mistletoe(Viscum album ssp.austriacum)in Scots pine stands has increased in many European countries.Understanding the factors that influence the occurrence of mistletoe in stands is key to making appropriate forest management decisions to limit damage and prevent the spread of mistletoe in the future.Therefore,the main objective of this study was to determine the probability of mistletoe occurrence in Scots pine stands in relation to stand-related endogenous factors such as age,top height,and stand density,as well as topographic and edaphic factors.We used unmanned aerial vehicle(UAV)imagery from 2,247 stands to detect mistletoe in Scots pine stands,while majority stand and site characteristics were calculated from airborne laser scanning(ALS)data.Information on stand age and site type from the State Forest database were also used.We found that mistletoe infestation in Scots pine stands is influenced by stand and site characteristics.We documented that the densest,tallest,and oldest stands were more susceptible to mistletoe infestation.Site type and specific microsite conditions associated with topography were also important factors driving mistletoe occurrence.In addition,climatic water balance was a significant factor in increasing the probability of mistletoe occurrence,which is important in the context of predicted temperature increases associated with climate change.Our results are important for better understanding patterns of mistletoe infestation and ecosystem functioning under climate change.In an era of climate change and technological development,the use of remote sensing methods to determine the risk of mistletoe infestation can be a very useful tool for managing forest ecosystems to maintain forest sustainability and prevent forest disturbance.

Aging Mongolian pine plantations face high risks of drought-induced growth decline:evidence from both individual tree and forest stand measurements

Discerning vulnerability differences among different aged trees to drought-driven growth decline or to mortality is critical to implement age-specific countermeasures for forest management in water-limited areas.An important species for afforestation in dry environments of northern China,Mongolian pine(Pinus sylvestris var.mongolica Litv.)has recently exhibited growth decline and dieback on many sites,particularly pronounced in old-growth plantations.However,changes in response to drought stress by this species with age as well as the underlying mechanisms are poorly understood.In this study,tree-ring data and remotely sensed vegetation data were combined to investigate variations in growth at individual tree and stand scales for young(9-13 years)and aging(35-52 years)plantations of Mongolian pine in a water-limited area of northern China.A recent decline in tree-ring width in the older plantation also had lower values in satellited-derived normalized difference vegetation indices and normalized difference water indices relative to the younger plantations.In addition,all measured growth-related metrics were strongly correlated with the self-calibrating Palmer drought severity index during the growing season in the older plantation.Sensitivity of growth to drought of the older plantation might be attributed to more severe hydraulic limitations,as reflected by their lower sapwood-and leaf-specific hydraulic conductivities.Our study presents a comprehensive view on changes of growth with age by integrating multiple methods and provides an explanation from the perspective of plant hydraulics for growth decline with age.The results indicate that old-growth Mongolian pine plantations in water-limited environments may face increased growth declines under the context of climate warming and drying.

Long-term changes in radial growth of seven tree species in the mixed broadleaf-Korean pine forest in Northeast China:Are deciduous trees favored by climate change?

The role of the temperate mixed broadleaf-Korean pine forest(BKF)in global biogeochemical cycles will depend on how the tree species community responds to climate;however,species-specific responses and vulner-abilities of common trees in BKF to extreme climates are poorly understood.Here we used dendrochronological meth-ods to assess radial growth of seven main tree species(Pinus koraiensis,Picea jezoensis,Abies nephrolepis,Fraxinus mandshurica,Phellodendron amurense,Quercus mongolica,and Ulmus davidiana)in an old-growth BKF in response to climate changes in the Xiaoxing’an Mountains and to improve predictions of changes in the tree species compo-sition.Temperature in most months and winter precipita-tion significantly negatively affected growth of P.jezoensis and A.nephrolepis,but positively impacted growth of P.koraiensis and the broadleaf species,especially F.mandshu-rica and U.davidiana.Precipitation and relative humidity in June significantly positively impacted the growth of most tree species.The positive effect of the temperature during the previous non-growing season(PNG)on growth of F.mandshurica and Q.mongolica strengthened significantly with rapid warming around 1981,while the impact of PNG temperature on the growth of P.jezoensis and A.nephrolepis changed from significantly negative to weakly negative or positive at this time.The negative response of radial growth of P.jezoensis and A.nephrolepis to precipitation during the growing season gradually weakened,and the negative response to PNG precipitation was enhanced.Among the studied species,P.koraiensis was the most resistant to drought,and U.davidiana recovered the best after extreme drought.Ulmus davidiana,P.jezoensis and A.nephrolepis were more resistant to extreme cold than the other species.Climate warming generally exacerbated the opposite growth patterns of conifer(decline)and broadleaf(increase)spe-cies.Deciduous broadleaf tree species in the old-growth BKF probably will gradually become dominant as warming continues.Species-specific growth-climate relationships should be considered in future models of biogeochemical cycles and in forestry management practices.

The variation in basal channels and basal melt rates of Pine Island Ice Shelf

In recent years,there has been a significant acceleration in the thinning,calving and retreat of the Pine Island Ice Shelf(PIIS).The basal channels,results of enhanced basal melting,have the potential to significantly impact the stability of the PIIS.In this study,we used a variety of remote sensing data,including Landsat,REMA DEM,ICESat-1 and ICESat-2 satellite altimetry observations,and Ice Bridge airborne measurements,to study the spatiotemporal changes in the basal channels from 2003 to 2020 and basal melt rate from 2010 to 2017 of the PIIS under the Eulerian framework.We found that the basal channels are highly developed in the PIIS,with a total length exceeding 450 km.Most of the basal channels are ocean-sourced or groundingline-sourced basal channels,caused by the rapid melting under the ice shelf or near the groundingline.A raised seabed prevented warm water intrusion into the eastern branch of the PIIS,resulting in a lower basal melt rate in that area.In contrast,a deepsea trough facilitates warm seawater into the mainstream and the western branch of the PIIS,resulting in a higher basal melt rate in the main-stream,and the surface elevation changes above the basal channels of the mainstream and western branch are more significant.The El Ni?o event in 2015–2016 possibly slowed down the basal melting of the PIIS by modulating wind field,surface sea temperature and depth seawater temperature.Ocean and atmospheric changes were driven by El Ni?o,which can further explain and confirm the changes in the basal melting of the PIIS.

Pteropine Orthoreovirus(PRV7S)does not Establish Persistent Infection in NP460 Cells but with Inflammatory Response Triggered

Pteropine orthoreovirus(PRV)is a nonenveloped double-stranded RNA(dsRNA)virus of the genus Orthoreovirus under the family Reoviridae.The PRV genome is composed of 10 dsRNA segments,including three large(L)segments,three medium(M)segments,and four small(S)segments surrounded by an icosahedral capsid[1].

Response of soil bacterial community to biochar application in a boreal pine forest

Boreal forests commonly suff er from nitrogen defi ciency due to low rate of nitrogen mineralization.Biochar may promote soil organic matter decomposition and accelerate nitrogen mineralization.In this study,Illumina NovaSeq sequencing combined with functional annotation of prokaryotic taxa(FAPROTAX)analysis was used to investigate the eff ect of biochar pyrolysis temperatures,the amount of applied biochar,and the period since the biochar application(2-and 3-year)on soil bacterial communities.The results show that biochar pyrolysis temperatures(500℃ and 650℃)and the amount of applied biochar(0.5 kg m^(−2)and 1.0 kg m^(−2))did not change soil properties.Nevertheless,the interaction of biochar pyrolysis temperature and the amount had signifi cant eff ects on bacterial species richness and evenness(P<0.05).The application of biochar produced at 500℃ had a lower abundance of Actinobacteria and Verrucomicrobia,while that produced at 650℃ had a higher abundance of Conexibacter and Phenylobacterium.When biochar produced at 650℃ was applied,applying 0.5 kg m^(−2)had a higher abundance of Cyanobacteria,Conexibacter,and Phenylobacterium than that of 1.0 kg m^(−2)(P<0.05).Functionally,the abundance of the aromatic compound degradation group increased with the extension of application time and increase of pyrolysis temperature.The time since application played an important role in the formation of soil bacterial communities and their functional structure.Long-term studies are necessary to understand the consequence of biochar on bacterial communities in boreal forests.

Changes in the growth of Scots pine(Pinus sylvestris L.)stands in an urban environment in European Russia since 1862

A century and a half ago,in the European part of Russia(Moscow),forest scientists established the first long-term observations of forests,many of which are under observation to the present day.In the twentieth century,climate changes and,due to industrial development,forest areas were under air pollution.Based on observations from 157 sites,this study shows that the growth and density of Scots pine(Pinus sylvestris L.)have decreased by the end of the twentieth century.The environment has changed,and the growth and development of plantations has slowed.Pine yields(-34%stand volume)and their life expectancy(on average 50 years)have decreased.These changes are critical for forestry and for the management of forests in urban centers.Due to the decline in growth,ecosystem functions by urban forests has decreased.In order to increase the sustainability of urban forests and the provision of ecosystem services,it is preferable to create multi-species stands with a complex structure.

Effect of acid-associated mechanical pretreatment on the hydrolysis behavior of pine sawdust in subcritical water

The effects of sulfuric acid-associated mechanical pretreatment on the hydrolysis behavior of pine sawdust were investigated in this study.Sulfuric acid could act as an acidic catalyst to depolymerize holocellulose through cleavage of the glycosidic bonds,the dissociation energies of which were supplied by the impact of a ball on pine sawdust,during milling.The destruction of glycosidic and hydrogen bonds in pine sawdust resulted in a decrease of crystallinity and an increase of water solubility.The sulfuric acid could promote the hydrolysis of holocellulose and its hydrolysis products.It also destroyed the chemical linkages between holocellulose and lignin during ball milling.The cleavage of chemical linkages with holocellulose made lignin more difficult to hydrolyze in subcritical water,and higher activation energy was needed to hydrolyze pretreated pine sawdust at higher reaction temperatures.It also led to the formation of glucose char and aromatic-linked polymer char from the hydrolysis products of holocellulose.

Nail Holding Performance of Self-Tapping Screws on Masson Pine and Chinese Fir Dimension Lumbers

Screw connection is a type most commonly applied to timber structures.As important commercial tree species in China,Masson pine and Chinese fir have the potential to prepare wood structures.In this study,the effects of the diameter of the self-tapping screw and the guiding bores on the nail holding performance on different sections of Masson pine and Chinese fir dimension lumbers were mainly explored.The results showed that:(1)The nail holding strength of the tangential section was the maximum,followed by that of the radial section,and that of the cross section was the minimum.(2)The nail holding strength of Masson pine was higher than that of Chinese fir.(3)The nail holding strength grew with the increase in the diameter of self-tapping screws,but a large diameter would lead to plastic cracking of the timber,thus further affecting the nail holding strength.Masson pine and Chinese fir reached the maximum nail holding strength when the diameter of self-tapping screws was 3.5 mm.(4)Under a large diameter of screws,prefabricated guiding bores could mitigate timber cracking and improve its nail holding strength.(5)Prefabricated guiding bores were more necessary for the screw connection of Masson pine.The results obtained could provide a scientific basis for the screw connection design of Masson pine and Chinese fir timber structures.

Dynamics of mediterranean pine forests reforested after fires

Forest fires are frequent under a Mediterranean climate and have shaped the landscape of the region but are currently altered by human action and climate change.Fires have historically conditioned the presence of pine forests,depending on severity and forest regeneration.Regeneration of Mediterranean pine forests is not always successful,and a transition to shrublands or stands of resprouting species can occur,even after reforestation.This study analyses vegetation changes in two Mediterranean pine forests after severe fires and both reforested.The pines had difficulty to regenerate,even despite post-fire reforestation.The problem is the difficulty of young seedlings to survive,possibly due to increased summer drought.Problems are greater in pine species at the limit of their ecological tolerance:Pinus pinea had a much better recovery success while P.sylvestris and P.nigra virtually disappeared.Pinus pinaster had intermediate results but recovery was generally poor.A transition has taken place in many burnt areas to scrubland or to thickets of the resprouting Quercus rotundifolia,although it is not possible to know whether they will evolve into forests or remain in a sub climatic state.Resprouting species may increase fire severity but facilitates post-fire colonisation.Post-fire recovery difficulties are closely linked to issues of natural regeneration.Fire could initiate the disappearance of pine forests,but even in the absence of fire they may disappear in the long-term due to the lack of regeneration.Action is needed to increase the resilience of these forests,ensuring natural regeneration,and incorporating resprouting species in the understorey.

Microwave Drying of Scots Pine Lumber:Structure Changes,Its Effect on Liquid Permeability

Microwave(MW)drying method was adopted to enhance the liquid permeability of Scots pine lumber.Structure changes were characterized by stereoscope microscope,scanned electron microscope(SEM)and transmission electron microscope(TEM)examination.Pore parameters before and after MW treatment were detected by mercury intrusion porosimetry(MIP)method,and the effect of structure changes on liquid permeability were analyzed.As stereoscope microscope,SEM and TEM examination results showed,macro and micro checks mainly developed at intercellcular of tracheids,intercellular of ray parenchyma and tracheid,while these checks extend main along the radial-longitudinal plane.Pit border destruction,aspirated pits’orus translocation and micro-checks in tracheid cell wall were also observed.MIP test shown that pore volume and pore area increased as macro and micro checks generated to form new cavities.Microstructure changes would increase the quantity of pores or enlarge the pore diameter.Liquid flow pathways increased as macro and micro checks generated,aspirated pits reopened to form new pathways;liquid flow efficiency improved as porosity,pore volume and pore diameter increased which facilitated the liquid flow.

The morphological changes of basal channels based on multisource remote sensing data at the Pine Island Ice Shelf

The basal channel is a detailed morphological feature of the ice shelf caused by uneven basal melting.This kind of specifically morphology is widely distributed in polar ice shelves.It is an important research object of sea-ice interaction and plays a vital role in studying the relationship between the ice sheet/ice shelf and global warming.In this paper,high-resolution remote sensing image and ice penetration data were combined to extract the basal channel of the Pine Island Ice Shelf.The depth variation of Pine Island Ice Shelf in the recent 20 years was analyzed and discussed by using ICESat-1,ICESat-2,and IceBridge data.Combined with relevant marine meteorological elements(sea surface temperature,surface melting days,circumpolar deep water and wind)to analyze the basal channel changes,the redistribution of ocean heat is considered to be the most important factor affecting the evolution and development of the basal channel.

In Situ Generation of Copper Nanoparticles in Heat-Treated Copper-Containing Masson’s Pine as a Preservative Process for Sawn Timber

Heat-treated wood has good dimensional stability,durability,and color,but its susceptibility to fungal growth affects its commercial value.In this study,lumber harvested from mature Masson’s pine(Pinus massoniana Lamb.)was vacuum impregnated with a basic copper salt solution(copper hydroxide,diethanolamine,and polyethylene glycol 200)prior to heat-treatment at 220℃ for 3 h.Antifungal properties,surface chemistry,crystal structure and sugar contents were tested,compared with heat treatment alone.The results showed that the samples treated by heating without copper salt treatment showed poor suppression of fungal growth,the copperimpregnated heat-treated wood suppressed(100%)the growth of Botryodiplodia theobromae Pat.,Aspergillus niger V.Tiegh.,Penicillium citrinum Thom,and Trichoderma viride Pers.The combined results of X-ray photoelectron spectroscopy,X-ray diffraction and sugars analysis suggested that fungal inhibition by the heat-treated copper-bearing Masson’s pine was mainly due to the reduction of the metal salt by PEG200 at high temperature to generate copper nanoparticles.In addition,the reduced sugar content of the treated timber,and hence the nutrient substrate for spoilage microbes,reduced in the presence of the metal salts at high-temperature.This study has demonstrated an effective method of increasing low-grade wood’s utility and commercial value.

Analysis of Influencing Factors of Pine Wood Nematode Disease in Lu an City Based on GIS

[Objectives]The paper was to figure out the distribution of Monochamus alternatus in the stumps of pine wood nematode infected wood in Lu'an City and to provide a theoretical and practical basis for differentiated prevention and control of pine wood nematode disease in the future.[Methods]The factors influencing the number of epidemic small classes and infected plants in all counties and districts of Lu'an City were analyzed by GIS method,and the number of worms and wormholes of M.alternatus inside and outside the stumps of pine wood nematode infected wood in Lu'an City were investigated on the spot.[Results]There were 98,40,54,781,193,268 and 34 epidemic small classes of pine wood nematode disease,and 7241,6099,9532,39161,11079,49876 and 4853 infected plants in Yu'an District,Jin'an District,Yeji District,Shucheng County,Huoshan County,Jinzhai County and Huoqiu County,respectively.Pine wood nematode disease in Lu'an City mostly occurred in Huoshan County,Shucheng County and Jinzhai County,with relatively high altitude,paddy soil,the annual precipitation higher than 2062 mm,and the annual average temperature lower than 16.2℃.The number of diseased plants of pine wood nematode disease was positively correlated with altitude and annual precipitation,and was negatively correlated with annual average temperature.The number of worms and wormholes in pine wood nematode infected wood was very small at different altitudes,stump heights and stump diameters.[Conclusions]The pine wood nematode infected wood can be differentially processed after stump treatment,and stumps can be peeled.The results will provide a theoretical and practical basis for differentiated prevention and control of pine wood nematode disease in the future.

松籽油水酶法提取及酶法破乳工艺研究

以压榨松籽饼粕为原料,利用水酶法提取松籽油,通过单因素及响应曲面试验研究松籽油提取工艺,并对提油过程中产生的乳状液进行破乳研究分析。结果表明,碱性蛋白酶酶解效果最佳,最优工艺为酶解时间2.5 h,温度56℃,pH值11.3,加酶量5045 U·g^(-1),此条件松籽油提油率为71.73%。经比较碱性蛋白酶添加量为3000 U·g^(-1)时破乳效果最好,破乳率为87.68%,在酶法破乳后进行酸化处理,通过调节pH值至5时破乳率提高至92.18%,此条件下松籽油得油率为70.36%。

甘肃产不同品种松针的抗氧化活性研究

以甘肃产白皮松、雪松、油松、落叶松、樟子松和华山松6个树种的松针为研究对象,采用DPPH法、ABTS法和铜离子还原能力测定其不同浓度乙醇提取物的体外抗氧化活性,并采用熵值法综合评价遴选出抗氧化活性强的松针资源,为松针的进一步开发利用提供参考。研究表明:采用不同浓度乙醇提取时,40%乙醇提取物的抗氧化活性最好;不同品种松针中,雪松松针的抗氧化活性最好,其中天水产雪松松针40%乙醇提取物的评分最高达99.52%,表明其可以作为进一步研究松针抗衰老研究的资源。

HPLC-DPPH法评价雪松松针抗氧化活性成分

目的评价雪松松针抗氧化活性成分。方法以维生素C(VC)和没食子酸为阳性对照,采用高效液相色谱(HPLC)法测定1,1-二苯基-2-苦肼基(DPPH)溶液加入雪松松针抗氧化活性成分后DPPH峰面积的衰减情况,并通过计算DPPH自由基的清除率和半数抑制浓度(IC_(50)),来评价雪松松针3种有效部位及8个单体成分的抗氧化活性。采用Agilent Eclipse Plus-C_(18)(250 mm×4.6 mm,5μm)为色谱柱,乙腈-水(62∶38,V/V)为流动相;检测波长为517 nm;流速为1.0 mL/min;柱温为25℃;进样量为20μL。结果雪松松针各有效部位清除DPPH自由基活性的能力大小依次为总黄酮>总多酚>总木脂素,IC_(50)分别为76.10、100.50、115.40μg/mL。与阳性对照比较,除山柰酚-3-O-β-D-葡萄糖苷外,7个单体化合物清除DPPH自由基活性的能力大小依次为二氢杨梅素>金丝桃苷>原儿茶酸>没食子酸>阿魏酸>杨梅素>山柰酚-3-O-(6″-O-E-阿魏酰基)-β-D-吡喃葡萄糖苷>VC>异鼠李素,IC50分别为16.50、19.40、23.73、27.24、32.10、32.20、47.60、93.20、297.20μg/mL。结论建立了HPLC-DPPH法用于评价雪松松针抗氧化活性;雪松松针具有较强的抗氧化活性,其中有效部位总黄酮、总多酚及单体成分二氢杨梅素、金丝桃苷等是具有开发前景的纯天然抗氧化剂。

灰葡萄孢寄生菌Clpse01的杀线活性研究

在用灰葡萄孢菌Botrytis cinerea培养松材线虫的过程中,发掘了一株具有杀线活性的灰葡萄孢寄生菌Clpse01。本文在光学显微镜下对菌株Clpse01的形态和其对灰葡萄孢菌的生长影响进行了观察;采用ITS4/ITS5、LR5/LR0R、Bt2a/Bt2b和EF1-688F/EF1-1251R引物进行了分子生物学鉴定;同时测试了菌株Clpse01对松材线虫的杀线活性及对松材线虫卵孵化能力和繁殖能力的影响;最后测试了菌株Clpse01的胞外蛋白浓度,胞外β-葡萄糖苷酶活性和胞外几丁质酶活性。根据形态学和分子生物学结果,将菌株Clpse01鉴定为假卡鲁卡枝惠霉Clonostachyspseudochroleuca。菌株Clpse01可以缠绕、侵入灰葡萄孢菌的菌丝以获得营养来源,使灰葡萄孢菌的菌丝生长受到抑制。将2000条松材线虫接种到长满菌株Clpse01的培养基上,8 d后仅分离出6条/皿活线虫,而对照灰葡萄孢菌培养基上分离的线虫数为33066条/皿活线虫;菌株Clpse01发酵液处理松材线虫48 h后的校正死亡率为88%,浓度为1×108孢子/mL的孢子悬液处理松材线虫24 h后的校正死亡率为88.33%;菌株Clpse01 2×稀释发酵液24 h对卵的孵化抑制率为100%。发酵培养6 h后,菌株Clpse01胞外蛋白浓度为1782.917μg/mL,胞外β-葡萄糖苷酶为9.99 U/mL,胞外几丁质酶含量为0.8792U/mL,并随发酵时间推移而升高。菌株Clpse01通过寄生松材线虫的食物来源灰葡萄孢菌和影响松材线虫的繁殖、存活和卵的孵化起作用,胞外β-葡萄糖苷酶、几丁质酶是毒杀松材线虫的两个重要生理因子。

2%甲维盐乳油对松材线虫病的防效测定

[目的]为测试2%甲维盐乳油(商品名:松高飞)对松材线虫病的防治效果。[方法]首先通过室内浸渍法比较了2%甲维盐乳油和2%甲维盐微乳剂(商品名:林源松韵)的杀线活性,然后在温室和林间通过树干注射测试了2%甲维盐乳油对松材线虫病的防治效果,最后通过高效液相色谱法测定了不同时间、不同松树体内甲维盐的含量变化。[结果]室内浸渍法结果显示,2%甲维盐乳油具有更强的杀线活性,LC_(50)为41.808 mg·L^(-1),显著低于2%甲维盐微乳剂的75.959 mg·L^(-1)(p<0.05)。温室预防组结果显示,2%甲维盐乳油的预防效果显著,2 a后松树无死亡。温室治疗组结果显示,2%甲维盐乳油治疗时间越早,治疗效果越好;2 a后,接种线虫后7 d注药治疗组,松树无死亡;而30 d后注药治疗组,白皮松、无死亡,油松死亡率为20%,华山松死亡率为40%。林间的试验显示2%甲维盐乳油在两块林地的防治效果显著,其试验组马尾松林2 a累计死亡率分别为2%和5%,远低于对照组马尾松死亡率20%和25%。随着时间变化,不同松树体内甲维盐变化有所差异,华山松体内平均甲维盐含量最高(36.59 mg·kg^(-1)),油松最低(27.11 mg·kg^(-1))。注射后15 d内,所有松树体内甲维盐含量均上升;15 d后,华山松树干中部甲维盐含量逐渐升高,60 d的含量最高;而油松、白皮松树干内甲维盐含量逐渐降低并趋平。[结论]2%甲维盐乳油对松材线虫具有显著的杀线活性,温室和林间防治效果显著,在不同松树体内传输和存留效果良好,无明显药害,可以进一步推广使用。