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Selected physical and chemical properties of mechanically extracted neem seed oil sourced as a preservative for ligno-cellulose in south-western Nigeria
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作者 Andrew A. ERAKHRUMEN 《Forestry Studies in China》 CAS 2011年第4期263-269,共7页
Most of the seeds produced by neem (Azadirachta indica A. Juss) trees in Nigeria are currently underutilized. Hence, relevant literature provides only limited information conceming many of the seed oils from this co... Most of the seeds produced by neem (Azadirachta indica A. Juss) trees in Nigeria are currently underutilized. Hence, relevant literature provides only limited information conceming many of the seed oils from this country, especially where it concems the potential applications of these oils as preservatives for ligno-cellulose against bio-deterioration. Using standard procedures therefore, this study was carried out to evaluate and document selected physical and chemical properties of neem seed oil (NSO), mechanically extracted using a cold press at 31.03 N-mm^-2 pressure and a room temperature of 25 ± 2℃. The results show that oil yield was 38.42% with a specific gravity of 0.91 ± 0.01. The amount of acid was 18.24 ± 1.31 mg KOH.g^-1 and that of iodine 93.12 ± 2.01 g-100 g^- 1, while saponification and peroxide values were 172.88 ± 2.06 and 1.42 ± 0.04 mg·g^-1 respectively. The implication of the values obtained, particularly those for the chemical properties, as they concern the potential application of NSO as a preservative for ligno-eellulose, is likely that it may be useful in this regard since the values may support some of the documented anti-microbial properties of the oil, although other physical and chemical properties that may affect this potential are recommended for investigations. Conclusions and other recommendations follow in line with the results of the study. 展开更多
关键词 neem seed oil physical and chemical properties mechanical extraction cold press
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印楝种子提取物对荔枝蝽的毒性及与其等位酶基因型之间的关系(英文) 被引量:7
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作者 卢芙萍 赵冬香 +2 位作者 刘业平 王爱萍 陈青 《昆虫学报》 CAS CSCD 北大核心 2006年第2期241-246,共6页
用5.2mg/mL(LC50)的印楝种子提取物对荔枝蝽1龄若虫进行急性毒性处理,24h死亡率为51.8%。通过等位酶分析检测了死亡与存活试虫两种酶(PGI和MDH),两个基因座(Pgi和Mdh)上各基因型及等位基因与印楝种子提取物毒性之间的关系,进行致死性差... 用5.2mg/mL(LC50)的印楝种子提取物对荔枝蝽1龄若虫进行急性毒性处理,24h死亡率为51.8%。通过等位酶分析检测了死亡与存活试虫两种酶(PGI和MDH),两个基因座(Pgi和Mdh)上各基因型及等位基因与印楝种子提取物毒性之间的关系,进行致死性差异比较研究。结果表明,印楝种子提取物对具有不同基因型及等位基因个体的致死性存在差异。在Pgi基因座上,Pgi-bb基因型死亡率最高,为84%,Pgi-aa和Pgi-cc基因型死亡率较低,分别为0和7%,且与死亡率最高的Pgi-bb基因型存在显著差异(P<0.05)。在基因座Mdh上,Mdh-aa基因型个体死亡率最高(93%),而具有Mdh-cc基因型的个体全部存活了下来,另外3个基因型Mdh-ab、Mdh-bb与Mdh-bc死亡率居中,都与Mdh-aa、Mdh-cc基因型死亡率之间存在显著差异。在等位基因上,Pgi-a和Mdh-c个体的死亡率都最低,与各自其他两个等位基因的死亡率之间存在显著差异。结果说明不同基因型个体对印楝提取物具有不同的反应,印楝种子提取物对荔枝蝽等位酶基因型及等位基因存在选择性致死作用。这种荔枝蝽对印楝种子提取物的敏感性与其等位酶基因型及等位基因之间显明的相关关系提示我们,可将荔枝蝽种群中对印楝种子提取物敏感性低的基因型及等位基因作为遗传标记去监测荔枝蝽对印楝种子提取物的抗性状况。 展开更多
关键词 荔枝蝽 印楝种子提取物 等位酶 基因型 等位基因 选择性致死作用
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楝树籽提取物对土壤供氮和氮肥利用率的影响 被引量:1
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作者 张晓晓 沈其荣 +1 位作者 谈建康 茆泽圣 《应用生态学报》 CAS CSCD 2002年第11期1417-1420,共4页
研究了楝树籽不同方式的提取物 (NⅠ ,NⅡ )对普通铁质淋溶土壤、简育水耕人为土壤供N和N肥利用率的影响 .NⅠ可在一定程度上抑制这两种土壤产生NO3 N ,NⅡ在两种土壤上前期均能显著固定NH4+ N ,并在后期释放出NH4+ N .如果NⅠ和NⅡ ... 研究了楝树籽不同方式的提取物 (NⅠ ,NⅡ )对普通铁质淋溶土壤、简育水耕人为土壤供N和N肥利用率的影响 .NⅠ可在一定程度上抑制这两种土壤产生NO3 N ,NⅡ在两种土壤上前期均能显著固定NH4+ N ,并在后期释放出NH4+ N .如果NⅠ和NⅡ 2种提取物同时与化肥N施入土壤 ,则可明显改善土壤的供N状况 ,使之与作物需N过程更吻合 .盆栽实验证明 ,NⅡ可显著提高肥熟旱耕人为土壤上作物对N肥的利用率 . 展开更多
关键词 楝树籽提取物 土壤供氮 氮肥利用率 影响 固定 矿化 氮肥增效作用 硝化抑制
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印楝种子粗提物诱导烟草炭疽病抗性的生理效应 被引量:1
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作者 胡亚杰 韦建玉 +3 位作者 王丽晶 陈吉荣 张纪利 江定心 《南方农业学报》 CAS CSCD 北大核心 2019年第3期524-532,共9页
【目的】研究印楝诱抗烟草炭疽病的机理,为烟草炭疽病的生物防治提供理论依据。【方法】测定根施印楝种子粉末对烟草炭疽病的防效,采用实时荧光定量PCR(qRT-PCR)检测印楝种子粉末处理后烟草的NTF6、NtLox、NtSGT1、NtPAL、NtPDF、NPR1、... 【目的】研究印楝诱抗烟草炭疽病的机理,为烟草炭疽病的生物防治提供理论依据。【方法】测定根施印楝种子粉末对烟草炭疽病的防效,采用实时荧光定量PCR(qRT-PCR)检测印楝种子粉末处理后烟草的NTF6、NtLox、NtSGT1、NtPAL、NtPDF、NPR1、NtRar1和PR1a等8个相关抗病基因的表达变化;对比叶面喷施不同印楝种子提取物(甲醇、乙酸乙酯和石油醚)溶液对烟草抗氧化酶活性的影响,并测定叶面喷施印楝种子甲醇提取物不同分离流分处理的烟叶总酚含量及抗炭疽病效果。【结果】根施印楝种子粉末可使烟草炭疽病的病情指数(26.67%)较对照(74.17%)显著降低(P<0.05,下同)。qRT-PCR检测结果表明,NPR1、NtRar1、NtSGT1和PR1a等4个抗病基因的相对表达量与印楝诱导烟草抗炭疽病相关。印楝种子甲醇提取物对烟草抗氧化酶活性的影响最大,其丙酮流分段在处理后6和96 h诱导烟草的总酚含量较对照明显提高,分别为对照的2.52和3.34倍,且其诱导烟草抗炭疽病的能力最强,病情指数(25.00%)显著低于对照(69.17%)。【结论】印楝种子提取物通过诱导提高PPO和POD抗病相关酶活性及诱导NPR1、NtRar1、NtSGT1和PR1a等抗病相关基因的表达产生抗炭疽病作用,且诱导活性成分主要存在于甲醇提取物的丙酮流分,其可作为烟草炭疽病的诱抗剂推广应用于烟草生产。 展开更多
关键词 烟草 炭疽病 印楝种子粗提物 诱导抗性 抗性机理
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印楝种子中杀虫活性成分的分离提取研究 被引量:8
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作者 彭黎旭 冯信平 +1 位作者 吴莉宇 符悦冠 《热带作物学报》 CSCD 2001年第4期55-60,共6页
将印楝种子中杀虫活性成分分离成油溶性和水溶性2个混合物后,再用萃取分离法分别将各混合物分离为3个小组,含活性物质个数较多的小组用快速低压液相色谱法分离为124个极性不同的产物,分离产物中的活性物质个数用薄层色谱法鉴定,对有代... 将印楝种子中杀虫活性成分分离成油溶性和水溶性2个混合物后,再用萃取分离法分别将各混合物分离为3个小组,含活性物质个数较多的小组用快速低压液相色谱法分离为124个极性不同的产物,分离产物中的活性物质个数用薄层色谱法鉴定,对有代表性的6个分离产物进行杀虫活性初步试验。结果表明,本试验中,用于提取和萃取印楝种仁中的杀虫活性成分的有机溶剂的搭配是目前较好的一种,能达到油溶性活性成分与油脂基本分离;用快速低压液相色谱法分离的印楝杀虫活性物质具有很高的杀虫选择性和广谱杀虫性。 展开更多
关键词 印楝种子 杀虫活性成分 分离 提取 杀虫效果
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均匀设计在印楝粗提物提取工艺研究中的应用
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作者 韩丙军 何书海 彭黎旭 《热带农业科学》 2006年第2期29-32,共4页
用均匀设计试验方法研究印楝粗提物的提取。选择石油醚Ⅰ萃取的用量和时间、甲醇萃取的用量和时间、乙酸乙酯萃取的用量和时间作为主要影响因子,以乙酸乙酯粗提物质量作为试验检测指标,通过国际通用统计软件SAS对试验结果进行分析,得出... 用均匀设计试验方法研究印楝粗提物的提取。选择石油醚Ⅰ萃取的用量和时间、甲醇萃取的用量和时间、乙酸乙酯萃取的用量和时间作为主要影响因子,以乙酸乙酯粗提物质量作为试验检测指标,通过国际通用统计软件SAS对试验结果进行分析,得出最佳提取工艺:石油醚Ⅰ萃取的用量80mL、时间348min,甲醇萃取的用量360mL、时间720min,乙酸乙酯萃取的用量110mL、时间20min。在此工艺条件下,20g印楝种仁得到印楝粗提物产品1.6041g,产品提取率为8.02%。 展开更多
关键词 均匀设计 印楝粗提物 提取工艺
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Effects of Manual, Cultural, Botanical and Chemical Treatments of Termite Control in Hamelmalo Agricultural College Area
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作者 Biniam Efriem Habteab Goitom +2 位作者 Rayet Idris Yosief Girmay Adungna Haile 《Open Journal of Ecology》 2021年第1期64-74,共11页
Termites are the most serious pests of field and horticultural crops, forests, and wooden household furniture. In Hamelmalo Agricultural College (HAC) the infestation of termite is very high resulting in great destruc... Termites are the most serious pests of field and horticultural crops, forests, and wooden household furniture. In Hamelmalo Agricultural College (HAC) the infestation of termite is very high resulting in great destruction of crop plants and wooden office and dormitory furniture. The devastating attack of termites should be managed by using best and ecofriendly management method. The purpose of the study was to compare the effectiveness of manual destruction of mounds and killing of termite queen and king, chemical chlorpyrifos, seed and leaves extract of neem and Lantana (as separate experiment) and smoke on termite control. Termite mounds were selected randomly inside HAC compound. The materials used were hand hoe, spade, fork, water, 20 L jar and protective clothes. The treatments were replicated three times. The botanical treatments were prepared at 2 L of highly concentrated extracts per 20 L of water each. Chlorpyrifos was applied at 20 ml per 20 L of water. Dried woody plants were used for smoke treatment. Careful digging was done to avoid king escape and queen rupture and they were killed by burning. Among all, the mechanical destruction and killing of king and queen and chlorpyrifos resulted in a complete control of the termite population. Except in the mounds treated by chlorpyrifos, the activity of termite population was very active and they closed the opened galleries immediately after treatment even though there were dead termite castes in all treatments. Living termite castes were counted by taking a medium size spade of broken mound pieces. The highest count was recorded from mounds treated by smoke. After two weeks the queen and king in every treatment mound were cheeked and killed for those who were alive. Except by the chlorpyrifos and manual destruction of mound (king and queen were killed before) all the royal families were alive and killed. Controlling of termite population in the field (outside their mound) is not possible due to the hidden foraging activity of termites, environmental safety from chemicals and the high egg laying potential of the queen. Finding the best alternative to control from their source mound for the mound building termites resulted in effective control of the population by manual destruction of mounds and killing of queen and king and chlorpyrifos. By the side effect of chlorpyrifos to untargeted organisms and the time consuming and laborious method of manual destruction of mounds, selection to the best from these two control measure is almost the same. 展开更多
关键词 Termite Castes MOUNDS CULTURAL Mechanical Chlorpyrifos Lantana seed and Leaf extract neem Leaf and seed Kernel extract
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印楝种仁粗提物体外抗鸭瘟病毒活性的研究 被引量:2
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作者 徐娇 宋旭 +5 位作者 殷中琼 程安春 贾仁勇 刘颖彘 叶奎川 邓云夏 《中国兽医科学》 CAS CSCD 北大核心 2011年第12期1302-1307,共6页
采用索氏提取法及溶剂萃取法,用950mL/L乙醇提取印楝种仁并萃取,分别得石油醚萃取物、乙酸乙酯萃取物及水层。采用细胞病变(CPE)法及四甲基偶氮唑盐(MTT)比色法研究三种萃取物对鸭瘟病毒(DPV)的抑制作用。结果显示,石油醚萃取物、乙酸... 采用索氏提取法及溶剂萃取法,用950mL/L乙醇提取印楝种仁并萃取,分别得石油醚萃取物、乙酸乙酯萃取物及水层。采用细胞病变(CPE)法及四甲基偶氮唑盐(MTT)比色法研究三种萃取物对鸭瘟病毒(DPV)的抑制作用。结果显示,石油醚萃取物、乙酸乙酯萃取物及水层对细胞的最大无毒浓度分别为0.007 8、0.003 9和0.125 0mg/mL;当其浓度为0.000 5、0.000 3及0.156 0mg/mL时,对DPV的抑制率各为13.23%、29.24%和11.72%。结果表明,三种萃取物对DPV均有不同程度的抑制作用,其中乙酸乙酯萃取物效果较好。 展开更多
关键词 印楝种仁提取物 鸭瘟病毒 抗病毒活性
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