对采自新疆库车县胡杨林地的野生木耳蘑菇进行菌丝体分离,获得一株白色菌丝.应用引物ITSlf和ITS4扩增其ITS区间,测序且结果经BLAST比对和构建NJ进化树来确定其分类地位.结果显示该菌丝(XJURML-3)为灰白翅孢壳Emericellopsis pallida(L.A...对采自新疆库车县胡杨林地的野生木耳蘑菇进行菌丝体分离,获得一株白色菌丝.应用引物ITSlf和ITS4扩增其ITS区间,测序且结果经BLAST比对和构建NJ进化树来确定其分类地位.结果显示该菌丝(XJURML-3)为灰白翅孢壳Emericellopsis pallida(L.A.Beljakova),GenBank检索号EU045572.XJURML-3形态学特征与国际已有Emericellopsis pallida strain CBS 624.73较为相似,菌株在中国典型培养物保藏中心保藏号为:CCTCC AF 207025.展开更多
目的:分析不同物种FADD的蛋白序列进化规律及FADD在不同组织中的转录和表达分布特征。方法:使用Mega 5.0软件对主要物种中FADD的蛋白序列进行比对分析,根据蛋白序列相似性使用邻近法绘制进化树。取FADD+/-小鼠脑、肝、肾、心、肺、肌肉...目的:分析不同物种FADD的蛋白序列进化规律及FADD在不同组织中的转录和表达分布特征。方法:使用Mega 5.0软件对主要物种中FADD的蛋白序列进行比对分析,根据蛋白序列相似性使用邻近法绘制进化树。取FADD+/-小鼠脑、肝、肾、心、肺、肌肉、脾脏、胃、肠,分别提RNA并反转录成cDNA和同时制备相应的蛋白样品,将FADD+/-小鼠在核酸水平和蛋白水平各主要组织中FADD的表达结果与小鼠FADD表达的UniGene EST Profile结果进行综合比较分析。结果:获得不同物种FADD的蛋白序列比对结果及进化规律,确定FADD在小鼠肺、心脏、胃中相对高表达的可靠结果,其他组织中FADD的表达量还有待进一步验证。结论:FADD的蛋白序列在进化中相对比较保守,其在小鼠肺、心脏、胃组织中高表达,这为进一步研究FADD在不同组织中生物功能奠定了良好的基础。展开更多
Traditionally, Chinese indigenous cattle is geographically widespread. The present study analyzed based on genome-wide variants to evaluate the genetic background among 157 individuals from four representative indigen...Traditionally, Chinese indigenous cattle is geographically widespread. The present study analyzed based on genome-wide variants to evaluate the genetic background among 157 individuals from four representative indigenous cattle breeds of Hubei Province of China： Yiling yellow cattle （YL）, Bashan cattle （BS）, Wuling cattle （WL）, Zaobei cattle （ZB）, and 21 indi- viduals of Qinchuan cattle （QC） from the nearby Shanxi Province of China. Linkage disequilibrium （LD） analysis showed the LD of YL was the lowest （~=0.32） when the distance between markers was approximately 2 kb. Principle component analysis （PCA）, and neighbor-joining （NJ）-tree revealed a separation of Yiling yellow cattle from other geographic nearby local cattle breeds. In PCA plot, the YL and QC groups were segregated as expected; moreover, YL individuals clustered together more obviously. In the N J-tree, the YL group formed an independent branch and BS, WL, ZB groups were mixed. We then used the FST statistic approach to reveal long-term selection sweep of YL and other 4 cattle breeds. According to the selective sweep, we identified the unique pathways of YL, associated with production traits. Based on the results, it can be proposed that YL has its unique genetic characteristics of excellence resource, and it is an indispensable cattle breed in China.展开更多
基金国家自然科学基金项目(6074100160871092)+3 种基金黑龙江省杰出青年科学基金项目(JC200611)黑龙江省自然科学基金重点项目(ZJG0705)哈尔滨工业大学校基金项目(HIT.2003.53)the High-Tech Research and Development Program of China(2007AA01Z171)~~
文摘对采自新疆库车县胡杨林地的野生木耳蘑菇进行菌丝体分离,获得一株白色菌丝.应用引物ITSlf和ITS4扩增其ITS区间,测序且结果经BLAST比对和构建NJ进化树来确定其分类地位.结果显示该菌丝(XJURML-3)为灰白翅孢壳Emericellopsis pallida(L.A.Beljakova),GenBank检索号EU045572.XJURML-3形态学特征与国际已有Emericellopsis pallida strain CBS 624.73较为相似,菌株在中国典型培养物保藏中心保藏号为:CCTCC AF 207025.
文摘目的:分析不同物种FADD的蛋白序列进化规律及FADD在不同组织中的转录和表达分布特征。方法:使用Mega 5.0软件对主要物种中FADD的蛋白序列进行比对分析,根据蛋白序列相似性使用邻近法绘制进化树。取FADD+/-小鼠脑、肝、肾、心、肺、肌肉、脾脏、胃、肠,分别提RNA并反转录成cDNA和同时制备相应的蛋白样品,将FADD+/-小鼠在核酸水平和蛋白水平各主要组织中FADD的表达结果与小鼠FADD表达的UniGene EST Profile结果进行综合比较分析。结果:获得不同物种FADD的蛋白序列比对结果及进化规律,确定FADD在小鼠肺、心脏、胃中相对高表达的可靠结果,其他组织中FADD的表达量还有待进一步验证。结论:FADD的蛋白序列在进化中相对比较保守,其在小鼠肺、心脏、胃组织中高表达,这为进一步研究FADD在不同组织中生物功能奠定了良好的基础。
基金funded in part by the National Natural Science Foundation of China (31402039,31472079,31372294)the Beijing Natural Science Foundation (6154032)+2 种基金the Species and Breed Resources Conservation of the Ministry of Agriculture of China (2017-2019)the Cattle Breeding Innovative Research Team of Chinese Academy of Agricultural Sciences (cxgc-ias-03)the National Beef Cattle Industrial Technology System (CARS-37)
文摘Traditionally, Chinese indigenous cattle is geographically widespread. The present study analyzed based on genome-wide variants to evaluate the genetic background among 157 individuals from four representative indigenous cattle breeds of Hubei Province of China： Yiling yellow cattle （YL）, Bashan cattle （BS）, Wuling cattle （WL）, Zaobei cattle （ZB）, and 21 indi- viduals of Qinchuan cattle （QC） from the nearby Shanxi Province of China. Linkage disequilibrium （LD） analysis showed the LD of YL was the lowest （~=0.32） when the distance between markers was approximately 2 kb. Principle component analysis （PCA）, and neighbor-joining （NJ）-tree revealed a separation of Yiling yellow cattle from other geographic nearby local cattle breeds. In PCA plot, the YL and QC groups were segregated as expected; moreover, YL individuals clustered together more obviously. In the N J-tree, the YL group formed an independent branch and BS, WL, ZB groups were mixed. We then used the FST statistic approach to reveal long-term selection sweep of YL and other 4 cattle breeds. According to the selective sweep, we identified the unique pathways of YL, associated with production traits. Based on the results, it can be proposed that YL has its unique genetic characteristics of excellence resource, and it is an indispensable cattle breed in China.
