Some of the characteristics of post-modernism, such as opposition centers, denial of holism, identity, differences and uncertainties, have had a great impact on the view of education. Because the purpose of education ...Some of the characteristics of post-modernism, such as opposition centers, denial of holism, identity, differences and uncertainties, have had a great impact on the view of education. Because the purpose of education is the starting point and end-result of education, which is related to the general orientation of education development, we have to re-examine the concept of modem educational purpose in the post-modernism thought. The author holds that compared with the modem education view, the educational purpose view of the post-modem period shows the following characteristics： Maintaining the diversification and difference of individuahty, paying attention to cultivating social consciousness and critical ability, learning to understand and respect ecological harmony.展开更多
卟啉类化合物是一类重要的光化学材料,其衍生物特殊的光电特性在各个领域中得到了广泛的应用。利用密度泛函理论(DFT)研究了(free base porphyrin,FBP)及其异构体(neo-confused porphyrin,NECP)和(n-confused porphyrin,NCP)三种卟啉环...卟啉类化合物是一类重要的光化学材料,其衍生物特殊的光电特性在各个领域中得到了广泛的应用。利用密度泛函理论(DFT)研究了(free base porphyrin,FBP)及其异构体(neo-confused porphyrin,NECP)和(n-confused porphyrin,NCP)三种卟啉环的几何结构和分子轨道能级。采用TDDFT方法计算真空和溶剂场极化连续模型下三者的吸收光谱。计算表明由于N原子位置变化,FBP,NECP和NCP在Soret带和Q带两个特征吸收峰也有不同。按FBP,NECP和NCP顺序,分子轨道能级LUMO依次降低,HOMO轨道依次升高,从而造成吸收光谱红移。HOMO和HOMO-1轨道能级的分裂造成了FBP和NECP的Soret带的多个吸收峰,而NCP的LUMO和LUMO+1的能级差与其HOMO和HOMO-1能级差几乎相等造成Soret带只有一个最高吸收峰。计算结果表明不同溶剂(苯、氯仿、乙腈和水)条件下三者的Soret带和Q带特征吸收峰均有显著变化。为此重点讨论了N原子位置的变化及在不同性质溶剂下FBP,NCP和NECP三类化合物Soret带/Q带吸收光谱性质的变化规律和机理。展开更多
Our country enters a new era of post-modernity, highconcept and large data technology, which includes threemain features. Firstly, it’s vital to combine science withhumanities. Medical humanities gradually fade away ...Our country enters a new era of post-modernity, highconcept and large data technology, which includes threemain features. Firstly, it’s vital to combine science withhumanities. Medical humanities gradually fade away andreductionism in science field need to reform, concept ofholism also should be reacquainted. Secondly, peopleshould pay more attention to the evolution ofanthropological ontology, focus emphasis on the relevantstudies, and make their persistent efforts toward the realworld. Finally, based on the excellent and characteristicculture of the Chinese nation, absorbing other culture indifferent nationalities and regions will be moremeaningful, this kind of integrated approach also presentsmassive advantages for us.展开更多
Based on interpretation of post-modernism, basic design techniques of landscape architecture in China under the influence of post-modernism were summarized through analyzing its influence and comparing domestic and ov...Based on interpretation of post-modernism, basic design techniques of landscape architecture in China under the influence of post-modernism were summarized through analyzing its influence and comparing domestic and overseas scholars' understanding of post-modern landscape architecture. The study aims at providing scientific references for the future theoretical and practical researches on the design of post-modern landscape architecture in China.展开更多
There thought to be a great weakness in the construction of Angel in Tess for Angel's illogically changeable character.The author holds,however,that Angel's character is reasonable as he is a man in the Victor...There thought to be a great weakness in the construction of Angel in Tess for Angel's illogically changeable character.The author holds,however,that Angel's character is reasonable as he is a man in the Victorian Age.This article tries to interpret the male subject deconstruction with post-modern theory to demonstrate the concern to the female in Hardy's work.展开更多
Chicago Millennium Park is a place where "post-modern architecture" concentrates, Cloud Gate, Crown Fountain and Jay Pritzker Music Pavilion are three representative post-modern architectures in the park. Mi...Chicago Millennium Park is a place where "post-modern architecture" concentrates, Cloud Gate, Crown Fountain and Jay Pritzker Music Pavilion are three representative post-modern architectures in the park. Millennium Park is an urban public space of Chicago City, its design concepts such as resource sharing and adaptation to local conditions are worth learning.展开更多
Central European musicians, as a consequence of the efforts of several important figures, have contributed to the development of jazz in the second half of the 20th century. Jazz pianists in Europe were a distinctive ...Central European musicians, as a consequence of the efforts of several important figures, have contributed to the development of jazz in the second half of the 20th century. Jazz pianists in Europe were a distinctive part of the world jazz scene and made significant achievements during the communist era. They were able to master the European classical piano school, becoming virtuoso pianists themselves, as well as acquiring self-taught jazz skills. Semi-isolated from the contemporary music trends abroad, Central European artists nonetheless made remarkable musical accomplishments that deserve to be called the Central European Jazz School. Between 1948 and 1989, the communist ideology controlled every country of Central Europe including East Germany. The careers of jazz pianists in these countries would have developed differently if they had lived under democratic conditions. This is evident in the lives of those who emigrated to Western countries and subsequently found a place in the world jazz scene. Using analysis of the Central European jazz musicians＇ compositions and their professional careers, this paper aims to demonstrate the extent to which the Central European Jazz School has contributed to jazz music in Europe and the wider world.展开更多
Starting from the current situation of contemporm-y media advertising, this paper summarizes negative thoughts and emotions such as doubt, boredom and negation in contemporary media advertising. It also deconstructs t...Starting from the current situation of contemporm-y media advertising, this paper summarizes negative thoughts and emotions such as doubt, boredom and negation in contemporary media advertising. It also deconstructs these negative thoughts and emotions from the perspective of social culture and concludes that our country＇s social and cultural ideas have advantages in solving them.展开更多
Chinese literary anthropology, growing out of comparative literature and cross-cultural studies at the end of the 20th century, is a notably innovative research paradigm in contemporary Chinese humanities and enters a...Chinese literary anthropology, growing out of comparative literature and cross-cultural studies at the end of the 20th century, is a notably innovative research paradigm in contemporary Chinese humanities and enters a new stage at the beginning of the 21st century. Firstly, this essay attempts to interpret how Chinese literary anthropology responds accordingly to the new development of international anthropology and mythology, updates the postmodern concept of myth knowledge of Confucian myth and the pattern of the mythological knowledge, thereby constructing the core in modern Chinese context. Afterward, it discusses the differences between the great tradition and the little tradition, and the significance of returning to the sacred context. Last, the focus of archetypal criticism in the context of Chinese anthropology has moved from literary concerns to cultural concerns and employing the quadruple evidences and five kinds of narratives to comprehensively interpret Confucian classics.展开更多
Oncolytic virus(OV)is increasingly being recognized as a novel vector in cancer immunotherapy.Increasing evidence suggests that OV has the ability to change the immune status of tumor microenvironment,so called transf...Oncolytic virus(OV)is increasingly being recognized as a novel vector in cancer immunotherapy.Increasing evidence suggests that OV has the ability to change the immune status of tumor microenvironment,so called transformation of‘cold’tumors into‘hot’tumors.The improved anti-tumor immunity can be induced by OV and further enhanced through the combination of various immunomodulators.The Neo-2/15 is a newly de novo synthesized cytokine that functions as both IL-2 and IL-15.However,it specifically lacks the binding site of IL-2 receptorαsubunit(CD25),therefore unable to induce the Treg proliferation.In present study,a recombinant vesicular stomatitis virus expressing the Neo-2/15(VSVM51R-Neo-2/15)was generated.Intratumoral delivery of VSVM51R-Neo-2/15 efficiently inhibited tumor growth in mice without causing the IL-2-related toxicity previously observed in clinic.Moreover,treatment with VSVM51R-Neo-2/15 increased the number of activated CD8t T cells but not Treg cells in tumors.More tumor-bearing mice were survival with VSVM51R-Neo-2/15 treatment,and the surviving mice displayed enhanced protection against tumor cell rechallenge due to the induced anti-tumor immunity.In addition,combination therapy of OV and anti-PD-L1 immune checkpoint inhibitors further enhanced the anti-tumor immune response.These findings suggest that our novel VSVM51R-Neo-2/15 can effectively inhibit the tumor growth and enhance the sensitivity to immune checkpoint inhibitors,providing promising attempts for further clinical trials.展开更多
Corynebacterium glutamicum represents an emerging recombinant protein expression factory due to its ideal features for protein secretion,but its applicability is harmed by the lack of an autoinduction system with tigh...Corynebacterium glutamicum represents an emerging recombinant protein expression factory due to its ideal features for protein secretion,but its applicability is harmed by the lack of an autoinduction system with tight regulation and high yield.Here,we propose a new recombinant protein manufacturing platform that leverages ethanol as both a delayed carbon source and an inducer.First,we reanalysed the native inducible promoter PICL from the acetate uptake operon and found that its limited capacity is the result of the inadequate translation initial architecture.The two strategies of bicistronic design and ribozyme-based insulator can ensure the high activity of this promoter.Next,through transcriptional engineering that alters transcription factor binding sites(TFBSs)and the first transcribed sequence,the truncated promoter PA256 with a dramatically higher transcription level was generated.When producing the superfolder green fluorescent protein(sfGFP)under 1%ethanol conditions,PA256 exhibited substantially lower protein accumulation in prophase but an approximately 2.5-fold greater final yield than the strong promoter PH36.This superior expression mode was further validated using two secreted proteins,camelid antibody fragment(VHH)and endoxylanase(XynA).Furthermore,utilizing CRISPRi technology,ethanol utilization blocking strains were created,and PA256 was shown to be impaired in the phosphotransacetylase(PTA)knockdown strains,indicating that ethanol metabolism into the tricarboxylic acid cycle is required for PA256 upregulation.Finally,this platform was applied to produce the“de novo design”protein NEO-2/15,and by introducing the N-propeptide of CspB,NEO-2/15 was effectively secreted with the accumulation 281 mg/L obtained after 24 h of shake-flask fermentation.To the best of our knowledge,this is the first report of NEO-2/15 secretory overexpression.展开更多
Within the sphere of contemporary social sciences,the terms“modernity,”“post-modernity”and“globalization”have penetrated,as the core concepts,into various fields of social sciences in a logical way.In constituti...Within the sphere of contemporary social sciences,the terms“modernity,”“post-modernity”and“globalization”have penetrated,as the core concepts,into various fields of social sciences in a logical way.In constituting the concept of“modernity,”sociology of education develops the educational theory,as sociological theory does,into a“grand narrative”and“foundationalist”theory;the contribution of post-modernity is pluralism and self-examination in an attempt to transcend modernity.Globalization,a kind of expanded modernity,makes education sociologists broaden their perspective from single and traditional“nationality,”“society”and“nation”to an international society and even to the global society,which has broken through the research paradigm of modernity with ethnocentrism,anthropocentrism and Euro-centrism.These changes have required urgent constitution of the conceptions and theoretical frameworks of sociology of education to be applied on a global level.展开更多
In natural language, number-words can be used in two different syntactic ways: adjectivally, i.e., with the syntactic status of an adjective, as in (1) 4Mars has two moons/ and nominally, i.e., with the syntactic stat...In natural language, number-words can be used in two different syntactic ways: adjectivally, i.e., with the syntactic status of an adjective, as in (1) 4Mars has two moons/ and nominally, i.e., with the syntactic status of a noun phrase, as in (2)‘Two is even.’ This syntactic difference is often taken to correspond to a difference in semantic function: adjectival number-words function as predicables, whereas nominal number-words function as singular terms. The view that nominal number-words function as singular terms is typically supported by appeal to certain tests for singular-termhood, tests that stem from the (neo-)Fregean tradition. In this paper, I argue that these tests do not support the view of nominal number-words as singular terms.展开更多
文摘Some of the characteristics of post-modernism, such as opposition centers, denial of holism, identity, differences and uncertainties, have had a great impact on the view of education. Because the purpose of education is the starting point and end-result of education, which is related to the general orientation of education development, we have to re-examine the concept of modem educational purpose in the post-modernism thought. The author holds that compared with the modem education view, the educational purpose view of the post-modem period shows the following characteristics： Maintaining the diversification and difference of individuahty, paying attention to cultivating social consciousness and critical ability, learning to understand and respect ecological harmony.
文摘卟啉类化合物是一类重要的光化学材料,其衍生物特殊的光电特性在各个领域中得到了广泛的应用。利用密度泛函理论(DFT)研究了(free base porphyrin,FBP)及其异构体(neo-confused porphyrin,NECP)和(n-confused porphyrin,NCP)三种卟啉环的几何结构和分子轨道能级。采用TDDFT方法计算真空和溶剂场极化连续模型下三者的吸收光谱。计算表明由于N原子位置变化,FBP,NECP和NCP在Soret带和Q带两个特征吸收峰也有不同。按FBP,NECP和NCP顺序,分子轨道能级LUMO依次降低,HOMO轨道依次升高,从而造成吸收光谱红移。HOMO和HOMO-1轨道能级的分裂造成了FBP和NECP的Soret带的多个吸收峰,而NCP的LUMO和LUMO+1的能级差与其HOMO和HOMO-1能级差几乎相等造成Soret带只有一个最高吸收峰。计算结果表明不同溶剂(苯、氯仿、乙腈和水)条件下三者的Soret带和Q带特征吸收峰均有显著变化。为此重点讨论了N原子位置的变化及在不同性质溶剂下FBP,NCP和NECP三类化合物Soret带/Q带吸收光谱性质的变化规律和机理。
文摘Our country enters a new era of post-modernity, highconcept and large data technology, which includes threemain features. Firstly, it’s vital to combine science withhumanities. Medical humanities gradually fade away andreductionism in science field need to reform, concept ofholism also should be reacquainted. Secondly, peopleshould pay more attention to the evolution ofanthropological ontology, focus emphasis on the relevantstudies, and make their persistent efforts toward the realworld. Finally, based on the excellent and characteristicculture of the Chinese nation, absorbing other culture indifferent nationalities and regions will be moremeaningful, this kind of integrated approach also presentsmassive advantages for us.
文摘Based on interpretation of post-modernism, basic design techniques of landscape architecture in China under the influence of post-modernism were summarized through analyzing its influence and comparing domestic and overseas scholars' understanding of post-modern landscape architecture. The study aims at providing scientific references for the future theoretical and practical researches on the design of post-modern landscape architecture in China.
文摘There thought to be a great weakness in the construction of Angel in Tess for Angel's illogically changeable character.The author holds,however,that Angel's character is reasonable as he is a man in the Victorian Age.This article tries to interpret the male subject deconstruction with post-modern theory to demonstrate the concern to the female in Hardy's work.
文摘Chicago Millennium Park is a place where "post-modern architecture" concentrates, Cloud Gate, Crown Fountain and Jay Pritzker Music Pavilion are three representative post-modern architectures in the park. Millennium Park is an urban public space of Chicago City, its design concepts such as resource sharing and adaptation to local conditions are worth learning.
文摘Central European musicians, as a consequence of the efforts of several important figures, have contributed to the development of jazz in the second half of the 20th century. Jazz pianists in Europe were a distinctive part of the world jazz scene and made significant achievements during the communist era. They were able to master the European classical piano school, becoming virtuoso pianists themselves, as well as acquiring self-taught jazz skills. Semi-isolated from the contemporary music trends abroad, Central European artists nonetheless made remarkable musical accomplishments that deserve to be called the Central European Jazz School. Between 1948 and 1989, the communist ideology controlled every country of Central Europe including East Germany. The careers of jazz pianists in these countries would have developed differently if they had lived under democratic conditions. This is evident in the lives of those who emigrated to Western countries and subsequently found a place in the world jazz scene. Using analysis of the Central European jazz musicians＇ compositions and their professional careers, this paper aims to demonstrate the extent to which the Central European Jazz School has contributed to jazz music in Europe and the wider world.
文摘Starting from the current situation of contemporm-y media advertising, this paper summarizes negative thoughts and emotions such as doubt, boredom and negation in contemporary media advertising. It also deconstructs these negative thoughts and emotions from the perspective of social culture and concludes that our country＇s social and cultural ideas have advantages in solving them.
文摘Chinese literary anthropology, growing out of comparative literature and cross-cultural studies at the end of the 20th century, is a notably innovative research paradigm in contemporary Chinese humanities and enters a new stage at the beginning of the 21st century. Firstly, this essay attempts to interpret how Chinese literary anthropology responds accordingly to the new development of international anthropology and mythology, updates the postmodern concept of myth knowledge of Confucian myth and the pattern of the mythological knowledge, thereby constructing the core in modern Chinese context. Afterward, it discusses the differences between the great tradition and the little tradition, and the significance of returning to the sacred context. Last, the focus of archetypal criticism in the context of Chinese anthropology has moved from literary concerns to cultural concerns and employing the quadruple evidences and five kinds of narratives to comprehensively interpret Confucian classics.
文摘Oncolytic virus(OV)is increasingly being recognized as a novel vector in cancer immunotherapy.Increasing evidence suggests that OV has the ability to change the immune status of tumor microenvironment,so called transformation of‘cold’tumors into‘hot’tumors.The improved anti-tumor immunity can be induced by OV and further enhanced through the combination of various immunomodulators.The Neo-2/15 is a newly de novo synthesized cytokine that functions as both IL-2 and IL-15.However,it specifically lacks the binding site of IL-2 receptorαsubunit(CD25),therefore unable to induce the Treg proliferation.In present study,a recombinant vesicular stomatitis virus expressing the Neo-2/15(VSVM51R-Neo-2/15)was generated.Intratumoral delivery of VSVM51R-Neo-2/15 efficiently inhibited tumor growth in mice without causing the IL-2-related toxicity previously observed in clinic.Moreover,treatment with VSVM51R-Neo-2/15 increased the number of activated CD8t T cells but not Treg cells in tumors.More tumor-bearing mice were survival with VSVM51R-Neo-2/15 treatment,and the surviving mice displayed enhanced protection against tumor cell rechallenge due to the induced anti-tumor immunity.In addition,combination therapy of OV and anti-PD-L1 immune checkpoint inhibitors further enhanced the anti-tumor immune response.These findings suggest that our novel VSVM51R-Neo-2/15 can effectively inhibit the tumor growth and enhance the sensitivity to immune checkpoint inhibitors,providing promising attempts for further clinical trials.
基金This work received funding from the National Natural Science Foundation of China(No.21878124,22078128,and 21938004)the Fundamental Research Funds for the Central Universities(No.JUSRP221032)+1 种基金the 111 Project(No.111-2-06)the national first-class discipline program of Light Industry Technology and Engineering(LITE2018-24).
文摘Corynebacterium glutamicum represents an emerging recombinant protein expression factory due to its ideal features for protein secretion,but its applicability is harmed by the lack of an autoinduction system with tight regulation and high yield.Here,we propose a new recombinant protein manufacturing platform that leverages ethanol as both a delayed carbon source and an inducer.First,we reanalysed the native inducible promoter PICL from the acetate uptake operon and found that its limited capacity is the result of the inadequate translation initial architecture.The two strategies of bicistronic design and ribozyme-based insulator can ensure the high activity of this promoter.Next,through transcriptional engineering that alters transcription factor binding sites(TFBSs)and the first transcribed sequence,the truncated promoter PA256 with a dramatically higher transcription level was generated.When producing the superfolder green fluorescent protein(sfGFP)under 1%ethanol conditions,PA256 exhibited substantially lower protein accumulation in prophase but an approximately 2.5-fold greater final yield than the strong promoter PH36.This superior expression mode was further validated using two secreted proteins,camelid antibody fragment(VHH)and endoxylanase(XynA).Furthermore,utilizing CRISPRi technology,ethanol utilization blocking strains were created,and PA256 was shown to be impaired in the phosphotransacetylase(PTA)knockdown strains,indicating that ethanol metabolism into the tricarboxylic acid cycle is required for PA256 upregulation.Finally,this platform was applied to produce the“de novo design”protein NEO-2/15,and by introducing the N-propeptide of CspB,NEO-2/15 was effectively secreted with the accumulation 281 mg/L obtained after 24 h of shake-flask fermentation.To the best of our knowledge,this is the first report of NEO-2/15 secretory overexpression.
文摘Within the sphere of contemporary social sciences,the terms“modernity,”“post-modernity”and“globalization”have penetrated,as the core concepts,into various fields of social sciences in a logical way.In constituting the concept of“modernity,”sociology of education develops the educational theory,as sociological theory does,into a“grand narrative”and“foundationalist”theory;the contribution of post-modernity is pluralism and self-examination in an attempt to transcend modernity.Globalization,a kind of expanded modernity,makes education sociologists broaden their perspective from single and traditional“nationality,”“society”and“nation”to an international society and even to the global society,which has broken through the research paradigm of modernity with ethnocentrism,anthropocentrism and Euro-centrism.These changes have required urgent constitution of the conceptions and theoretical frameworks of sociology of education to be applied on a global level.
文摘In natural language, number-words can be used in two different syntactic ways: adjectivally, i.e., with the syntactic status of an adjective, as in (1) 4Mars has two moons/ and nominally, i.e., with the syntactic status of a noun phrase, as in (2)‘Two is even.’ This syntactic difference is often taken to correspond to a difference in semantic function: adjectival number-words function as predicables, whereas nominal number-words function as singular terms. The view that nominal number-words function as singular terms is typically supported by appeal to certain tests for singular-termhood, tests that stem from the (neo-)Fregean tradition. In this paper, I argue that these tests do not support the view of nominal number-words as singular terms.
