In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kid...In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kidney enlargement and white-spotted kidney. An isolate from the farm was identified as an avian infectious bronchitis virus （IBV） by chicken embryo inoculation, hemagglutination assay, virus interference assay, animal regression and tracheal rings culture. The complete ,S1 gene was amplified by RT-PCR, and its homology to that of the vaccine strains com- monly used in China was analyzed with DNAStar software. Therefore, the IBV isolate was initially classified into nephropathogenic IBV and named IBV JS09 strain.展开更多
文摘In November 2009, a respiratory disease with rapid transmission, rapid onset and mortality of about 8% appeared many times in a large chicken farm in Jiangsu Province of China. Necropsy revealed tracheal bleeding, kidney enlargement and white-spotted kidney. An isolate from the farm was identified as an avian infectious bronchitis virus （IBV） by chicken embryo inoculation, hemagglutination assay, virus interference assay, animal regression and tracheal rings culture. The complete ,S1 gene was amplified by RT-PCR, and its homology to that of the vaccine strains com- monly used in China was analyzed with DNAStar software. Therefore, the IBV isolate was initially classified into nephropathogenic IBV and named IBV JS09 strain.
